Labnet 222DS Bedienungsanleitung

222DS Benchtop Shaking Incubator

Instruction Manual

Catalog Numbers:

I-5222-DS

I-5222-DS-230V

Table of Contents

1.0 Introduction ...................................................................................1

2.0 Packing ........................................................................................1

3.0 Technical Description ...........................................................................1

3.1. Specifications ..............................................................................1

4.0 Safety Information .............................................................................2

5.0 Installation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2

5.1. Device Placement ..........................................................................2

5.2. Attaching the Power Cord...................................................................2

5.3. Attaching Shaking Platforms................................................................2

6.0 Operating Instructions..........................................................................3

6.1. Control Panel...............................................................................3

6.2. Basic Operation ............................................................................4

6.3 Changing Parameters During Operation......................................................6

6.4 Shaking Without Heating ...................................................................6

6.5 Heating Without Shaking ...................................................................6

7.0 Troubleshooting................................................................................7

8.0 Temperature Calibration ........................................................................7

9.0 Maintenance and Cleaning .....................................................................8

9.1 Cleaning of the Working Chamber (Painted Steel) ............................................8

9.2 Maintenance and Cleaning of the PMMA (Acrylic) Lid .........................................8

10.0 Positioning of Attachments on Shaking Platform.................................................9

11.0 Accessories. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9

12.0 Limited Warranty............................................................................. 10

13.0 Equipment Disposal .......................................................................... 10

Instruction Manual | 1

1.0 Introduction

The 222DS Benchtop Shaking Incubator is a combination product of a shaker and a temperature

chamber, designed for shaking and heat treatment of sensitive samples. It replaces two devices, reduces

time and space needed. It is very suitable for biochemistry, microbiology and medicine laboratories in

which thermal and shaking treatment of samples is a daily used routine.

2.0 Packing

The 222DS Benchtop Shaking Incubator includes the following components:

222DS Benchtop Shaking Incubator

Instruction Manual

3.0 Technical Description

The casing of Benchtop Shaking Incubator is made of steel plate, varnished with high resistant

polyurethane lacquer. Temperature chamber lid is made of PMMA, which is resistant to the temperatures

up to 90°C.

Basic equipment consists of independent shaking device and temperature chamber. Both sections are

driven and regulated via microcontroller driven electronics, which also controls all necessary sensors for

motor speed, temperature, lid, temperature safety fuse, time and self-diagnostics error levels in variety

of margin situations.

3.1. Specications

Heater Power 700W

Fan Power 17W

Shaking Motor Power 35W

Fuse 2 x 5A 250V

2 x 10A 115V

Environmental Temperature 5°C to 40°C

Relative Humidity Up to 85% non-condensing

Speed RPM Regulation Digital load independent, from 20 to 300 rpm in 1 rpm steps

Shaking Orbit 19 mm

Temperature Operating Range 5°C above room temperature to 70°C

Temperature Sensor PT100

Temperature Accuracy ±0.5°C

Temperature Stability ±0.5°C

Timer 1 min to 99 hours, timer HOLD function

Capacity 16 x 125 mL , 9 x 250 mL, 5 x 500 mL, 4 x 1,000 mL

Dimensions (W x D x H) 15 x 21 x 16 in. (37 x 53 x 40 cm)

Weight 45 lbs. (20.2 kg)

Overvoltage Category Installation category 1

2| 222DS BENCHTOP SHAKING INCUBATOR

4.0 Safety Information

Before cleaning disconnect the device from mains power supply. Device could be cleaned with a soft,

lightly wet cloth. Don’t use cleaners or liquids on aerosol basis.

Do not use the device nearby water sources. Take care, that water will not drop in the device, especially

by cleaning procedures.

Do not use device in aggressive atmosphere.

NOTE: In case that device is not functioning properly, even if you have exactly followed instructions

described in User’s Manual, you are allowed to use only those commands and procedures, which are

allowed by User’s Manual. Use of any other commands and procedure’s adjustments could result in

device destruction or longer service repair time. If the equipment is used in a manner not specified by

the manufacturer, the protection provided by the equipment may be impaired.

Do not shake flammable or explosive samples.

When closing the lid, do not put your hands between the lid and housing, because they can

get crushed.

When the lid is open, do not put your fingers in the area between the housing and

rotating platform. Danger of mechanical injury.

The lid may be hot when the device is working at higher temperatures. Take care not to burn

your hands.

The back side of the housing may be hot when the device is working at higher temperatures.

Take care not to burn your hands.

Power supply cord shall be with nominal cross section area of at least 18 AWG.

5.0 Installation

5.1. Device Placement

When selecting the right place for device, please consider following:

Put the device on smooth, horizontal and stable place.

Leave enough space beyond the device for normal air circulation, min. 15 cm.

Leave enough space around the device, min. 15 cm, for easy access and maintenance.

Avoid locations subject to shocks and vibrations.

Don’t use the device in surroundings, where there are fast temperature and humidity changes. Please

avoid also such places, which are under direct access of sunlight or places nearby heat producing devices.

Do not use the device in a flammable or explosive atmosphere.

NOTE: There has to be easy access to the shaker control panel and main plug in case of emergency.

5.2. Attaching the Power Cord

First connect the main power cord to the shaker then connect the plug end of the cord to a grounded

wall socket. Avoid lines on which powerful electric motors, refrigerators and similar devices are

connected.

Be careful when you plug the cord to a grounded wall socket.

Do not touch the plug with wet hands.

Do not pull the plug by the cord.

5.3. Attaching Shaking Platforms

The Shaking Incubator has a variety of platforms available, to meet most of the shaking needs. These

platforms mount to the shakers via four mounting platforms which easily plug into four rubber

mounting points on the top of the shaking incubator.

Instruction Manual | 3

6.0 Operating Instructions

6.1. Control Panel

88.8

STOP

Encoder

POWER

RUN

1 2 3

6 7

START

TEMP

SPEED

TIME

88.8

1. POWER key –switch ON (illuminates when on) or OFF.

2. RUN –green signal light illuminated when shaking incubator is running.

3. TEMPERATURE – yellow signal light illuminated when the shaking incubator is set on temperature.

4. SPEED – yellow signal light illuminated when the shaking incubator is set on speed.

5. TIME – yellow signal light illuminated when the shaking incubator is set on time.

6. ENCODER – by rotating the encoder clockwise (+) or counter clockwise (-) you can change the Time,

Speed and Temperature on the shaking incubator. Push encoder to change between Time, Speed and

Temperature. If you rotate encoder knob fast, the value on display goes up and down quickly.

7. START/STOP key – START or STOP operating.

8. TEMPERATURE display.

9. SPEED/TIME display.

4| 222DS BENCHTOP SHAKING INCUBATOR

6.2. Basic Operation

Press POWER switch on control panel. Shaking incubator automatically detects power supply frequency

F50 or F60 and shows it on display. Likewise the shaking incubator is then set to last saved parameters.

F50

STOP

Encoder

POWER

RUN

START

TEMP

SPEED

TIME

88.8

Press and hold encoder knob for more than 2 seconds. Temperature signal light blinks. With rotating

encoder clockwise (+) or counter-clockwise (-), set temperature to desired value:

37.5 = 37.5°C

99.5

STOP

Encoder

POWER

RUN

START

TEMP

SPEED

TIME

37.5

If you want to use the shaking incubator without temperature regulation set OFF on display. You get

OFF function on display, when you rotate encoder under 0.0°C or above 70.0°C.

OFF

STOP

Encoder

POWER

RUN

START

TEMP

SPEED

TIME

37.5

Press encoder knob.

Speed signal light blinks. With rotating encoder clockwise (+) or counter-clockwise (-), set rotating

speed to desired value:

34 = 34 rpm

120 = 120 rpm

STOP

Encoder START

120

POWER

RUN

TEMP

SPEED

TIME

37.5

Instruction Manual | 5

If you want to use the shaking incubator without shaking - set OFF on display. You get OFF function on

display, when you rotate encoder under 20 RPM or above 300 RPM.

Press encoder knob.

Time signal light blinks. With rotating encoder clockwise (+) or counter-clockwise (-), set time to

desired value from 1 min to 99 h:

70.3 = 70h 30 min

1.55 = 1h 55 min

0.01 = 1 min

STOP

Encoder START

0.01

POWER

RUN

TEMP

SPEED

TIME

37.5

If you want to set Time to hold (continued operation) rotate encoder under 0.01 min or above 99.0 h to

get “HLd” on display.

Press START/STOP key once to exit the changing of parameters. You are now in starting mode.

Only signal light of the last changed parameter shines.

NOTE: You always change the parameter, whose signal light blinks - temperature, speed or time.

You can always change one, two or three parameters. To exit the changing of parameters, always

press START/STOP key once.

Press START/STOP key to start the operation.

Run signal light shines. Shaking incubator counts down the time from set value.

You can move between the values of time and speed during the operation, by pressing the encoder.

These two values are shown alternately on bottom display.

STOP

Encoder START

0.01

POWER

RUN

TEMP

SPEED

TIME

37.5

6| 222DS BENCHTOP SHAKING INCUBATOR

6.3 Changing Parameters During Operation

NOTE: You cannot modify time during operation.

Temperature

Press and hold encoder knob for more than 2 sec. Temperature signal light blinks. Rotate encoder

clockwise (+) or counter-clockwise (-), to set the desired value. In the meantime, temperature signal

light pulses. When you set the new value, press START/STOP key only once. Shaking incubator goes

back to working parameters.

Speed

Press and hold encoder knob for more than 2 sec. If shaking incubator is not set on speed (speed signal

light is not blinking), press encoder – speed signal light must blink. Rotate encoder clockwise (+) or

counter- clockwise (-), to set the desired value. In the meantime speed signal light pulses. When you

set new value, press START/STOP key only once. Shaking incubator goes back to working parameters.

When time elapses or you press START/STOP key again, message End appears on display and Run signal

light pulses. When shaking incubator completely stops, it places itself to last used values for Time,

Speed and Temperature.

If you lift the lid during operation, shaking incubator stops immediately and message Opn

Lid appears on displays. When you close the lid again, shaking incubator starts operating

automatically.

If there is a power cut during operation, shaking incubator will restart automatically, as soon

as the electricity returns. Display will be flashing (warning you that the power was cut). You

can turn the flashing off, by pressing the encoder knob.

6.4 Shaking Without Heating

If you want to shake the samples without heating them, set Temperature to OFF.

6.5 Heating Without Shaking

If you want to heat the samples without shaking them, set Speed to OFF.

Instruction Manual | 7

7.0 Troubleshooting

The 222DS Shaking Incubator features built in self-diagnostic procedures which are constantly checking

the operating parameters and performance, as well as functions that are necessary for safe and reliable

operation. An error code is shown on the LCD display if an error occurs.

Sample of Error display:

E11

STOP

Encoder

POWER

RUN

START

TEMP

SPEED

TIME

Err

E11 This error message appears when something is wrong with motor regulation (PWM regulator,

pulse generator, motor). The shaking incubator is automatically stopped. Call for service.

E12 This error appears when the motor does not reach set RPM in 30 sec. The shaking incubator

is automatically stopped. Call for service.

E13 This error appears when set speed oscillates more than 100 rpm in 2 seconds.

Shaking incubator is automatically stopped. Call for service.

E21 The temperature sensor is not working properly with regards to the heater.

Shaking incubator is automatically stopped. Call for service.

E22 Temperature sensor notices a 5°C higher temperature than set temperature.

Shaking incubator is automatically stopped. Call for service.

E23 Set temperature is not reached in 2 hours. Shaking incubator is automatically stopped.

Call for service.

Should you have a question about the Labnet 222DS Shaking Incubator or required service for the unit,

contact Corning Customer service at 800.492.1110 or 978.442.2200. Do not send a unit for service

without first calling to obtain a repair authorization (RA) number. The unit should be properly packed to

avoid damage. Any damage resulting from improper packaging shall be the responsibility of the user.

8.0 Temperature Calibration

Shaking incubator has a software for temperature re-adjustment. First you have to measure the

temperature in the middle of the chamber, or even better in the sample, for minimum 2 hours. You

have to measure the temperature with a calibrated digital temperature thermometer, with precision

0.1°C or more. After 2 hours or more, read the temperature on thermometer and compare it with

the temperature on display of the shaking incubator. The difference between the temperatures on

thermometer and display is the value, which you enter in the software.

Example 1: Temperature on thermometer is 37.9°C, temperature on display is 37°C.

Difference is 37.9 – 37 = 0.9 This value 0.9, is the value (which you will enter into the software).

Example 2: Temperature on the thermometer is 36.2°C, temperature on display is 37°C.

Difference is 36.2 – 37 = -0.8. This value -0.8, is the value (which you will enter into the software).

Procedure for temperature calibration

1. Press and hold encoder knob for more than 5 seconds. Message “Cor” (Correction) appears on display.

2. Press encoder knob once to enter the value setting procedure and set the value with rotating encoder

counter-clockwise or clockwise (see Example 1 or Example 2). Pay attention to the sign of the value

(plus or minus). When you set the value, press encoder knob again.

3. Press the START/STOP key to finish the temperature re-adjustment.

NOTE: Temperature calibration should only be performed by qualified personnel.

8| 222DS BENCHTOP SHAKING INCUBATOR

9.0 Maintenance and Cleaning

9.1 Cleaning of the Working Chamber (Painted Steel)

The chamber should be cleaned regularly. Any samples which spill inside or outside the chamber must be

immediately wiped. You can only use mild cleaning solutions. Use only warm water. Usage of aggressive

or abrasive cleaners is not allowed.

For decontamination use cleaners with neutral Ph. Stainless steel platform can be decontaminated with

autoclave (120°C).

9.2 Maintenance and Cleaning of the PMMA (Acrylic) Lid

Maintenance:

Acrylic glass is sensitive to scratching. For cleaning, use only non-aggressive liquid detergents and

non-solids. Do not use the hard sponge, but only a soft cloth.

Grease and oil can be cleaned with hexane, petroleum or other chemicals with the aromatic base.

Cleaning:

For cleaning the lid, do not use the cleaners, which could damage the surface! Do not use: acetone,

benzene, paint thinner, carbon tetrachloride or other aggressive liquids, because they can damage

the surface of acrylic lid.

Do not use detergents containing ketones, esters and aromatics.

Do not use cleaners with solid and abrasive particles.

Do not use polishes.

Never clean the surface with detergent for cleaning nail polish (containing acetone or other solvents)

or with cleaning products for chemical cleaning, paint thinner, gasoline and similar. Avoid any contact

with these chemicals.

Do not use detergents with high levels of alcohol.

It is the best to use 2% aqueous solution of non-aggressive cleaning products (dishwashing detergent).

Wipe the surface with a lightly damp soft cloth.

Remove dust and dirt with dry soft cloth.

Take care when cleaning the device after operating, especially when you have used the device

at temperatures higher than 60°C. In such condition never touch the platform, because it can

be very hot.

Before you begin cleaning the device, unplug mains cord from power supply socket.

Before using any other cleaning or decontamination methods, except those recommended by the

manufacturer, you should check with the manufacturer, that the proposed method would not damage

the equipment.

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