Leica DM L Bedienungsanleitung

Leica DM L

Instructions

3rd edition, issued in 1998 by:

Leica Microsystems Wetzlar GmbH

Ernst-Leitz-Strasse

D-35578 Wetzlar, P.O. Box 2040

Responsible for contents:

Microscopy product management Phone +49(0)6441-292519

Fax +49(0)6441-292255

Important notes on this manual....................... 7

Assembly and description of components.... 8

Unpacking ............................................................ 8

Installation site ................................................... 8

Safety .................................................................... 8

Mains voltage, fuses .......................................... 9

Filters, polarizers................................................. 11

Condensers .......................................................... 11

Stages, tube system ........................................... 17

Incident light modules........................................ 18

Eyepieces, graticules......................................... 20

Objectives............................................................. 21

Light sources, lamp change.............................. 21

Performance parameters .................................. 27

Objectives............................................................. 27

Eyepieces ............................................................. 29

Tube system ......................................................... 31

Condensers .......................................................... 32

Total magnification ............................................. 33

Simple survey observation................................ 33

Operation.............................................................. 34

Basic setting for transmitted light ................... 34

Filters .................................................................... 36

Condensers, Koehler illumination.................... 37

Phase contrast .................................................... 42

Transmitted light darkfield................................. 44

Transmitted light polarization ........................... 46

Transmitted light interference contrast.......... 48

Incident light techniques................................... 52

Linear measurements ........................................ 58

Thickness measurements ................................. 59

TV microscopy..................................................... 60

Contents

Care and maintenance ...................................... 62

Wearing and spare parts, tools ....................... 63

Supplementary information.............................. 64

Tubes ..................................................................... 64

Intermediate modules ........................................ 64

Heating stages..................................................... 66

Viewing attachments/Tracing device ............. 67

Diapositive overlay ............................................. 68

Macro device....................................................... 70

Index...................................................................... 74

EU Conformity declaration ............................... 75

General specifications

The Leica DML microscope family

consists of a number of variants such as:

Leica DMLB (30 W lamp/with transformer supply/30 T)

020-519.500 to 020-519.503

Leica DMLB (30 W

lamp/with switched mode

power supply/

30 S)

020-519.504 to 020-519.507

Leica DMLB

(100Wlamp/with switched mode

power supply/10

0 S

)

020-519.507 to 020-519.511

Leica DMLP and DMLM

(100Wlamp/with switched mode

power supply/10

0 S

)

020-520.701 to 020-520.718

Distribution voltage (mains) 30 T 230/115/100 V ~ ±10%

(→p. 9): 30 S 90 – 250 V~

100 S 90 – 250 V~

Frequency: 50 – 60 Hz

Power consumption 30 T max. 145 VA

(→p. 9): 30 S max. 145 VA

100 S max. 160 W

Fuses: see page 63

Ambient temperature: 10 – 36°C

Relative humidity: 0 – 80 % up to 30°C

For inside use only

Overvoltage category: II

Pollution degree: 2

Transmitted light path

1Transmitted light source (30 or 100 W)

2Filter, permanently built-in

33 switchable filters*

4Field diaphragm

5Focusing

6Polarizer*

7Condenser, with disc*

8Aperture diaphragm

9Stage with specimen

10 Objectives

11 Tube slot (IC prisms*, compensator*)

12 Tube lens system in the tube

13 Tube prisms

14 Eyepiece (intermediate image,

14 field diaphragm)

* not part of all configurations

Incident light path*

16–20: Vertical illuminator*

15 Light source* (halogen, Hg or Xe)

16 Filter (BG 38)

17 Light stop

18 Aperture diaphragm

19 Field diaphragm

20 Filter system or incident light reflector

Cross section diagram of DAS Mikroskop LEICA DM LB

12b

12a

This manual is an integral part of the product

and must be read carefully before switching

on and using the microscope!

Text symbols and their meanings:

Numbers in brackets, e.g. (1.2),

refer to illustrations, in this

example Fig. 1, pos. 2.

Numbers with an arrow, e.g.

→p. 20, refer to a specific page

in this manual.

Special safety information is

printed on a grey background

and marked with the symbol

on the left.

Warning of hot surface.

n.b. Incorrect operation can

damage the microscope or

accessories.

Explanatory note.

Component is not part of every

configuration.

The Leica DML microscope series consists of

several basic stands and a range of modular

components allowing an almost unlimited

variety of individual outfits. Therefore this

manual has been given a modular layout as well

to show you other possible configurations

besides your own. It applies to the microscope

Leica DMLB(mainly for biological and medical

applications) and, together with a supple-

mentary manual, to the microscopes DMLP

(polarized light) and DMLM(material

examinations). The manual is divided into three

main chapters:

Assembly, Performance parameters, Operation

The manual is multilingual. Due to the spiral

binding you can turn the language you want

to the front. A foldable pocket-sized set of brief

instructions is also available in various

languages for the different basic stands, please

consult your supplier. A list of key data on

objectives, eyepieces, graticules and fluores-

cence filter cubes is also supplied with this

microscope. It is constantly being updated.

Special manuals are delivered with some addi-

tional equipment such as microscope cameras,

heating stages, and also in case of modi-

fications. We also print extensive brochures on

microscopy, which can be ordered, as can extra

copies of this manual, from our agencies for a

cover charge.

Important notes on this manual

(1.2)

→

p. 20

Attention:

ergonomically designed chair which can be

adjusted in several positions are the external

prerequisities for fatigue-free microscopy.

Safety

To guarantee that the microscope and

accessories are in a perfectly safe condition,

please note the following advice and

warnings: The mains plug must only be

inserted into a grounded outlet. If an

extension cord is used, it must be grounded

as well. Using the ground connection (1.7),

any accessories connected to the

microscope which have their own and/or a

different power supply can be given the same

ground conductor potential. Please consult

our servicing personnel if you intend to

connect units without a ground conductor.

The instruments and accessories described

in this manual have been safety-tested and

checked for possible hazards. Before

modifying the instrument in any way or

combining it with non-Leica products not

dealt with in this manual, it is essential to

consult the Leica agency for your area or the

main factory in Wetzlar!

Attention:

Unpacking, documents

Please compare the delivery carefully with the

packing note, delivery note or invoice. We

strongly recommend that you keep a copy of

these documents with the manual, so that you

have information on the time and scope of

delivery later when ordering more equipment or

when the microscope is serviced. Make sure

that no small parts are left in the packing

material. Some of our packing material has

symbols indicating environment-friendly

recycling.

When taking the microscope out of its packing

and putting it onto the desk, take care not to

damage the sensitive vibration-damping feet on

the bottom of the microscope.

On no account should you connect the

microscope and peripherals to the mains yet

→p. 9 (mains voltage) and p. 52 (incident

light only).

Installation site

Make sure that the workplace is free from oil

and chemical fumes. Vibrations, direct sunlight

and major temperature fluctuations have a

negative effect on measurements and

photomicrography. A stable desk of optimum

height (70 – 80 cm) is important. This and an

Attention:

Installation and safety

Attention:

Data, Setting the mains voltage

Some of the following data may vary according

to the model:

Built-in supply Type L 30 T (Fig. 1a)

Non-stabilized (major fluctuations in the mains

voltage may lead to visible falloff of image

brightness and colour shifts in photography).

Max. output 30 W (%12 V). Power consumption

45 VA.

Switchable voltage setting 230 V or 115 V (+10/

– 20 %) on the back of the microscope (1 and 2).

For users where the local mains voltage is

100 – 120 V: the microscope has been set at

230 V at the factory! Make sure to disconnect

from the mains before switching the voltage

(230 or 115 V)!

Attention:

4 7 9 10 8579108 6791083

Attention:

Transit protection

To avoid damage caused by vibration, take the

following precautions before transporting the

microscope: disassemble the objectives and

condenser (14.7). Remove the stage after

loosening the clamp screw (28.13). Block the

vertical movement of the stage bracket by fitting

hard foam rubber between the microscope

base, stage bracket and objective nosepiece.

Built-in power supply

The DML microscope series can be supplied

with 3 different built-in power supplies for 12 V

halogen lamps, depending on its intended

application.

Power supply

Fig. 1a–c Backs of DM L microscopes

aType L 30 T, b Type L 30 S, c Type L 100 S

1Type plate, 2115/230 V switch (type L 30 T only), 3Connection for 12 V 100 W lamp, type L 100 S only; some configurations also

have 2 100 W ports with switch, e.g. for alternate operation (transmitted/incident light), 412 V 30 W halogen lamp, 5Cover, only

for 30 W (removed in Fig. 3a), 6Adapter ring with clamp screw for 100 W lamphousing, 7Ground connection, 8Mains switch

with pilot lamp, 9Mains cable connection, 10 Fuse module with 2 fuses (type indicated on adhesive label)

abc

Built-in power supply Type L 30 S and

Type L 100 S (Figs. 1b and 1c)

Electronic stabilisation of output voltage %12 V.

Automatic adaptation to the local mains voltage

of 90 – 250 V, continuous. Max. output 30 or

100 W. Power consumption 45 or 160 W.

Fuses

All versions of the stand are equipped with

safety fuses on both phases. The fuse module

(1.10) can be removed with a sharp object.

Safety data →back of microscope (1.10); code

no. →p. 63.

The fuse ratings apply for all mains voltages.

Attention:

On no account should you use fuses of dif-

ferent ratings.

External power units

Assembly of lamphousings, lamp change

→

p. 21; 23.

Gas discharge lamps (Hg, Xe → p. 24) are

always fed by external power units. For incident

light, the 100 W halogen lamp can alternatively

also be run on an external power unit, e.g. for

simultaneous transmitted and incident light

illumination. A separate instruction manual is

supplied for external power units.

Fig. 2 Assembly tools

1Crosstip screwdriver*, 23 mm hexagonal screwdriver,

32 mm centering keys*, condenser UCL/UCLP, 41.5 mm

centering keys*, 53 mm Allen key*, 62.5 mm Allen key*

(short version)

Fig. 3 The main microscope components

* not part of all configurations

Eyepieces

Illuminator*/Intermediate system*

Objective

nosepiece

Specimen

stage

Condenser Transmitted

light

source

Tube

Stand

Microscope base

Reflected

light

source

2c 1b

Assembly tools

You only need a few ordinary screwdrivers to

assemble your microscope. These are supplied

with the delivery. Replacements for lost tools

can be obtained from us or from a tool shop

(Fig. 2, spare parts list, → p. 63).

Filter magazine* Transmitted light

Turn the microscope stand upside down (tube

and intermediate systems are not assembled!)

loosen the 3 screws (4.1) and lift off the base

plate.

Insert the filters (Ø 40 mm) into the semicircular

mounts (4.2). This does not have to be done in

any particular order. The name of the filter can

Filter holders for transmitted light

be seen on its edge, e.g. N 16. Stick corre-

sponding adhesive labels close to the operating

levers (4.5). Cf → 33.7, put filter magazine back

in position (4.1; 33.7).

Filter holder*

Attachment: 2 filters or Pol components with

32 mm diameter (32.5) can be inserted in the

filter holder (5.3; 32.1). The slit above the ICT/P

polarizer (6.3) is only for λ- or λ/4 compensator

(32.5).

Push the guide hole (5.3) into the guide pin at the

bottom (right) of the condenser holder and

attach holder with knurled screw (left) (5.1; 5.2).

Fig. 4 Fitting the filter magazine (trans-

mitted light)

1a, b, c Openings for fastening screws,

2Mounts for Ø 40 mm filters, 3Filter,

4Switch lever, 5Adhesive label

Fig. 5 Filter holder (mounting diameter

32 mm, →Fig. 32 and 33)

1Hole for attachment, 2Fastening screw

(above it; condenser centration

illustrated), 3Hole for guide pin, 4Filter

holder* with 2 filter mounts, 5Condenser

clamp screw

Fig. 6 Polarizer L ICT/P for transmitted

light

1Fastening screw, 2Polarizer, 3Mount

for λ- or λ/4 compensator, 4Polarizer

mount, rotatable

Inhaltsverzeichnis

Andere Leica Mikroskop Handbücher

Leica

Leica DMi1 Handbuch

Leica

Leica EZ4 Bedienungsanleitung

Leica

Leica DMPL Bedienungsanleitung

Leica

Leica SP5 confocal Bedienungsanleitung

Leica

Leica EZ5 Bedienungsanleitung

Leica

Leica DM IRB Bedienungsanleitung

Leica

Leica MC120 HD Bedienungsanleitung

Leica

Leica DM300 Bedienungsanleitung

Leica

Leica ATC 2000 Bedienungsanleitung

Leica

Leica DMIRE2 Bedienungsanleitung

Leica

Leica MATEO TL Bedienungsanleitung

Leica

Leica DMi8A Bedienungsanleitung

Leica

Leica S Series Bedienungsanleitung

Leica

Leica ES2 Bedienungsanleitung

Leica

Leica S7 E Bedienungsanleitung

Leica

Leica BF200 Bedienungsanleitung

Leica

Leica DM E Bedienungsanleitung

Leica

Leica PROVIDO Bedienungsanleitung

Leica

Leica DM750 M Bedienungsanleitung

Leica

Leica DM LSP Bedienungsanleitung

Leica

Leica DM1000 LED Bedienungsanleitung

Leica

Leica DMI6000B Bedienungsanleitung

Leica

Leica TCS SP5 X Bedienungsanleitung

Leica

Leica TCS SP8 Bedienungsanleitung

Beliebte Mikroskop Handbücher anderer Marken

Thermo Scientific

Thermo Scientific Nicolet iN10 Leitfaden

National

National DC3-163 Bedienungsanleitung

KERN Optics

KERN Optics OPE-1 Bedienungsanleitung

OPTIKA MICROSCOPES

OPTIKA MICROSCOPES B-150D Bedienungsanleitung

Prior

Prior Lux POL Bedienungsanleitung

Opitec

Opitec Smartscoop 115615 Bedienungsanleitung

KERN

KERN OPO-1 Bedienungsanleitung

Topcon

Topcon SP-1P Bedienungsanleitung

Motic

Motic PA43 Bedienungsanleitung

MshOt

MshOt MZ62 Bedienungsanleitung

KERN Optics

KERN Optics OZR-5 Bedienungsanleitung

VWR

VWR VisiScope 384 Series Bedienungsanleitung

Nikon

Nikon ECLIPSE E200 POL Bedienungsanleitung

ThermoFisher Scientific

ThermoFisher Scientific Continuµm Bedienungsanleitung

ThermoFisher Scientific

ThermoFisher Scientific Continuµm Bedienungsanleitung

Olympus

Olympus SZ61 Bedienungsanleitung

AO Cycloptic Bedienungsanleitung

Celestron

Celestron 44345 Bedienungsanleitung