Leica DMI3000B Bedienungsanleitung

Leica DMI3000B,

DMI4000B, DMI6000B

Instructions

Contents

6.10 Installation and Replacement of the

transmitted Light Lamps:

107 or 107/2 Lamp Housing........................ 45

6.11 Installing the Lamp Housing Mount

and Mirror Housing)................................... 46

6.12 Installation and Replacement of

Incident Light Lamps.................................. 48

6.13 Equipping the Incident Light

Turret Disk.................................................... 52

6.14 Inserting the Front Module Slider............ 55

6.15 Installation of the Polarizer

and Analyzer................................................ 55

6.16 Optional Accessories................................. 57

6.17 Connection to the Electronics Box.......... 58

6.18 Connection to the Computer..................... 59

6.19 Connection to the Power Supply ............. 59

7. Start-up ........................................................ 60

7.1 Functional Principle .................................. 60

7.2 Switching on the Microscope.................. 64

7.3 The LeicaDisplay ........................................ 65

7.4 The Function Buttons on the Stand......... 66

7.5 The SmartMove

Remote Control Module ............................ 69

7.6 Illumination.................................................. 69

7.6.1 Transmitted light .............................. 69

7.6.2 Incident Light - Fluorescence........ 73

7.7 Checking Phase Contrast Rings............... 74

7.8 Checking modulation contrast

slit diaphragms............................................ 77

7.9 Setting the Motorized Polarizer ............... 77

7.10 Adjusting the Light Sources ..................... 78

Contents

1. Important Notes about this Manual........ 7

2. Intended Purpose of the Microscope..... 8

3. Safety Notes................................................ 9

3.1 General Safety Notes ................................ 9

3.2 Electrical Safety.......................................... 10

3.3 Safety Instructions

for Handling the Light Sources ................ 12

3.4 Notes on handling laser devices ............. 12

3.5 Safety Instructions

for Handling Acids and Bases.................. 12

3.6 Disposal........................................................ 13

4. Overview of the Leica DMI Series.......... 14

5. Unpacking the Microscope ..................... 27

6. Assembling the Microscope.................... 30

6.1 Assembly Tools........................................... 30

6.2 Installation of the Transmitted Light

Illumination Carrier (TL)............................. 31

6.3 Installation of the DIC Module

and DIC Objective Prisms ......................... 32

6.4 Installation of Specimen Stages.............. 33

6.5 Installation of Condensers........................ 38

6.6 Installation of Eyepieces........................... 43

6.7 Installation of Objectives .......................... 43

6.8 Installation of Filters

in the Illumination Arm............................... 44

6.9 Installing the transmitted Light

Lamp Housing ............................................. 44

Contents

8. Operation..................................................... 81

8.1 Switching on................................................ 81

8.2 Contrast Methods....................................... 83

8.2.1 Bright Field (TL)................................ 83

8.2.2 Phase Contrast (TL) ....................... 85

8.2.3 Dark Field (TL) .................................. 86

8.2.4 Polarization (TL)............................... 87

8.2.5 Differential

Interference Contrast (TL) ............. 88

8.2.6 Integrated Phase Contrast (TL)..... 89

8.2.7 Integrated

Modulation Contrast (TL)................ 90

8.3 Fluorescence............................................... 91

8.4 Combination Methods ............................... 93

8.5 Focusing....................................................... 94

8.6 Tubes ............................................................ 96

8.7 Port selection ............................................. 96

8.8 Eyepieces..................................................... 97

8.9 Objectives.................................................... 98

8.10 Stages and Object Displacement ............ 101

8.11 Magniﬁcation Changer.............................. 102

8.12 Light sources............................................... 103

8.13 Aperture and Field Diaphragm ................ 104

9. Troubleshooting.......................................... 105

10. Care of the Microscope ............................ 109

10.1 Dust Cover ................................................... 109

10.2 Cleaning ....................................................... 109

10.3 Handling Acids and Bases........................ 110

11. Major Consumable

and Replacement Parts............................. 111

12. Dimensions.................................................. 112

13. Abbreviations and Pictograms................ 113

14. Index ............................................................ 115

15. EU Declaration of Conformity .................. 117

4. Overview of the Instruments

Fig. 1 Left side, Leica DMI4000 B and DMI6000 B

1Eyepiece

2Eyepiece tube

3Top port

4Intermediate pupil interface

5 LeicaScreen

6Light intensity

7Field diaphragm

8TL/IL switching

9Aperture diaphragm

10 Focus wheel (motorized Leica DMI6000 B,

manual (ﬁne and coarse) Leica DMI4000 B)

11 Variable function buttons

12 Left side port

13 Booster lens

(Leica DMI6000 B ﬂuorescence microscopes only)

14 Lamp mount (ﬂuorescence microscopes only)

15 Condenser head

16 Condenser base

17 Field diaphragm

18 Transmitted light lamp housing

19 DIC objective prism disk

678910111213

4. Overview of the Instruments

Fig. 2 R

ight side Leica DMI4000 B and DMI6000 B

1E-Focus buttons (Leica DMI6000 B only)

2Focus wheel (motorized Leica DMI6000 B,

manual (ﬁne) Leica DMI4000 B)

3Variable function buttons

4Opener for drawer (ﬂuorescence microscopes only)

5Drawer (ﬂuorescence microscopes only)

6Right side port

7Analyzer slot

8Centering window (ﬂuorescence microscopes only)

9Field diaphragm centering

(ﬂuorescence microscopes only)

Incident light lamp housing (ﬂuorescence microscopes

only)

11 Objective turret

12 Stage with attachable mechanical stage

12 3

6 78

9 10

4. Overview of the Instruments

Fig. 3 Front view Leica DMI4000 B and Leica DMI6000 B

1 LeicaScreen

2 Front control panel

3Port switching

4 Top port

5Manual transmitted light ﬁlters

6Bertrand lens centering

4. Overview of the Instruments

Fig. 4 General view Leica DMI4000 B and Leica DMI6000 B with SmartMove remote control module

Fig. 3b SmartMove remote control module

1Travel in x

2Travel in y

3 Focus

4Variable function buttons

(pre assigned at factory)

Fig. 3a Front control panel

1Fluorescence cube

2Shutter

3 100% light to all eyepieces

4 Port selection

5Magniﬁcation selection

61x tube lens

2543

7. Start-up

7.1 Functional Principle (Leica DMI4000 B and Leica DMI6000 B)

1. Intelligent Automation

• Switching between contrast methods at the touch of a button. Light rings, DIC prisms, etc.

are automatically positioned in the beam path.

• The microscope recognizes the selected objective and associated contrast method.

The intensity (INT), aperture diaphragm (AP) and ﬁeld diaphragm (FD) are always set to suit-

able values.

• The INT, AP and FD values are always based on the currently activated illumination axis

(transmitted light or incident light).

• The INT, AP, and FD values can be adjusted individually. Manual adjustments overwrite the

previous settings. The current setting is stored and is retained from one session to the next

when power is switched off.

2. Controls

• SmartMove knobs

for stage and focus control

• Fixed function buttons on stand

for INT, AP, and FD, as well as for switching between transmitted light and incident light axis

• Variable function buttons on stand, SmartMove, STP6000

These function buttons have functions suitable to the conﬁguration of your microscope as-

signed to them at the factory. The functions can be reprogrammed and/or adapted to your

speciﬁc requirements, however.

• Complete control of microscope and camera via software

(Leica Application Suite (LAS/LAS AF))

Thanks to its intelligent automation, the Leica DMI4000 B and DMI6000 B can be controlled using a

variety of control elements.

7. Start-up

Function Fixed Variable SmartMove Software

(DMI4000 B and DMI6000 B) Function Function Function Rotary and

buttons buttons buttons knobs STP6000

Stand Stand

4000 6000 4000 6000 4000 6000 4000 6000 4000/6000

Select contrast method - - + + + + - - +

Change transmitted light/incident light axis + + - - - - - - +

Change to objective - - - + - + - - +

Teach-in parfocality - - - - - - - - +

Change operating mode (dry/imm) - - + + + + - - +

Illumination Manager + + + + + + - - +

Magniﬁcation changer (motorized) + + - - - - - - +

Focusing - + - - - - - +1) +

Set stops - + - - - - - - +

Go to stop - + - - - - - - +

Change step increment (coarse/ﬁne) - - - + - + - - +

XY stage positioning - - - + +

Change speed - - - - - - - - +

Stage positions (store/go to) - - - - - - - - +

Change to ﬁlter/reﬂector cube + + (+) + + - - +

Side and bottom port (DMI6000 B only) + (+) + - +

DIC ﬁne adjustment + + - - - - - - +

Adapted Focus Control (AFC) - + - + - + - - +

+ always possible

(+) optional

- not possible

1) Focusing alternatively via wheels

7. Start-up

Possible Assignments for Variable Function Buttons on Stand and SmartMove

For Leica DMI4000 B and Leica DMI6000 B:

Function button Function

BF Bright ﬁeld transmitted light

PH Phase contrast transmitted light

ICT Interference contrast, transmitted light

DF Dark ﬁeld transmitted light

IMC Integrated modulation contrast

POL Polarization transmitted light

CHANGE TL Cycle through all contrast methods

INT ↑ Increase intensity (transmitted light)

INT ↓ Reduce intensity (transmitted light)

AP ↑Open aperture diaphragm (transmitted light)

AP ↓ Close aperture diaphragm (transmitted light)

FD ↑ Open ﬁeld diaphragm (transmitted light)

FD ↓ Close ﬁeld diaphragm (transmitted light)

SHUTTER TL Open/close TL shutter

TL FLT 1 Enable/disable transmitted light ﬁlter at position 1

TL FLT 2 Enable/disable transmitted light ﬁlter at position 2

FLUO Fluorescence (last ﬁlter cube)

CUBE 1-6 Select ﬁlter cube in position 1-6

CHANGE CUBE CW Change cube clockwise (1 → 4)

CHANGE CUBE CCW Change cube counterclockwise (4 → 1)

INT FLUO ↑ Increase intensity (ﬂuorescence)

INT FLUO ↓ Reduce intensity (ﬂuorescence)

FD FLUO ↑ Open ﬁeld diaphragm (ﬂuorescence)

FD FLUO ↓ Close ﬁeld diaphragm (ﬂuorescence)

CHG FW Toggle ﬁlter functions

IFW Activate external ﬁlter wheel

ExMan Activate Excitation Manager

SHUTTER FL Open/close ﬂuoshutter

COMBI Combination method (PH ﬂuorescence or ICT ﬂuorescence)

CHANGE COMBI Cycle through all combination methods

CHANGE OBJ CW Cycle through objectives clockwise

CHANGE OBJ CCW Cycle through objectives counterclockwise

Z FINE Activate ﬁne focusing (Leica DMI6000 B only)

Z COARSE Activate coarse focusing (Leica DMI6000 B only)

XY PRECISE Activate precise stage

XY FAST Activate fast stage

BTP ON/OFF Bottom port on/off (Leica DMI6000 B only)

DRY/IMM Switch dry/immersion

CHANGE FLT Switch TL ﬁlter

CHANGE CS Switch to confocal application

OBJ 1-6 Select objective at position 1-6

MEM 1-6 Memory activated stored functions

AFC ON/OFF Turns AFC on or off

AFC HOLD Holds current position

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