Leica FluoCombi III Bedienungsanleitung

Leica

FluoCombi III™

User manual

Leica FluoCombi III™ – User manual

Dear User,

Thank you for your confidence in our products. We trust you will enjoy them

and we wish you great success with the high-quality and high-performance

products from Leica Microsystems.

In developing our instruments, we place great value on simple, self-

explanatory operation. Nevertheless, please take the time to read the oper-

ating instructions so that you know the advantages and possibilities offered

by your stereomicroscope and can use them most efficiently. Should you

ever have any questions, please consult your local Leica representative.

We are gladly at your service. Customer service is a big thing with us. Not

only before the sale, but afterwards as well. You will find the address of

your nearest local representative, as well as valuable information about

Leica Microsystems products and services, from Leica Microsystems on

our web site at www.leica-microsystems.com.

Leica Microsystems (Switzerland) Ltd.

Stereo & Macroscope Systems

www.stereomicroscopy.com

3Leica FluoCombi III™ – Overviews

Overviews

Operating and function elements

1Fine focusing with mount thread for a 5×or 20×HR objective (requires an adapter)

2Clamping screw for locking the fine focusing

3Mechanism for parcentric swinging to the stereo or micro mode

4Slot for filter slide for dichroic mirror of your choice

5Mount for UV protection screen

Fig. 1 Leica FluoCombi IIITM, 5×planapo HR objective rotated into the beam path

6Clamping screw for fastening the UV protection screen

7Clamping screw for locking the UV protection screen in the desired tilt position

8Clamping screw for fastening the optics carrier in the microscope carrier of the FluoCombi III™

9Clamping screw for fastening the focusing stop

Leica FluoCombi III™ – Overviews

Fig. 2 Leica FluoCombi IIITM, 1×planapo objective rotated into the beam path

Leica FluoCombi III™ – User manual

Contents

Page

Overviews . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Operating and function elements . . . . . . . . . . . . 3, 4

1. General notes . . . . . . . . . . . . . . . . . . . . . . . . . . 6

1.1 The user manual . . . . . . . . . . . . . . . . . . . . . . . . 6

1.1.1 Diagrams . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

1.2 Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

2. Assembly

2.1 Expansion diagram . . . . . . . . . . . . . . . . . . . . . . 7

2.2 Standard delivery . . . . . . . . . . . . . . . . . . . . . . . 8

2.2.1 Additional components . . . . . . . . . . . . . . . . . . 8

2.3 Assembly sequence . . . . . . . . . . . . . . . . . . . . . 9

2.4 Column with focusing drive

and base of stand . . . . . . . . . . . . . . . . . . . . . . 10

2.5 Focusing stop . . . . . . . . . . . . . . . . . . . . . . . . . 10

2.6 Transport anchorage . . . . . . . . . . . . . . . . . . . 11

2.7 FluoCombi III™ and focusing drive . . . . . . . 11

2.8 Optics carrier . . . . . . . . . . . . . . . . . . . . . . . . . 12

2.9 Objectives . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

2.10 UV protection screen . . . . . . . . . . . . . . . . . . . 14

2.11 Dichroic mirrors . . . . . . . . . . . . . . . . . . . . . . . 14

2.12 Illumination . . . . . . . . . . . . . . . . . . . . . . . . . . . .14

3. Operation

3.1 Parfocality . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

3.2 Magnification . . . . . . . . . . . . . . . . . . . . . . . . . 17

4. Appendix

4.1 Weights . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

4.2 Dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . .19

4.3 Optical data . . . . . . . . . . . . . . . . . . . . . . . . . . .22

4.4 Technical data . . . . . . . . . . . . . . . . . . . . . . . . .24

Leica FluoCombi III™ – General notes

1. General notes

1.1 The user manual

Leica FluoCombi III™ is an accessory for the

Leica MZ16 F, MZ16 FA fluorescence stereomi-

croscopes. Together with your Leica FluoCombi III™,

you receive an interactive CD-ROM with the user

manual for all products from Leica Microsystems

(Switzerland) in the current EU languages. User

manuals and updates are also available for you

to download and print from our web site at

www.stereomicroscopy.com. The following

user manuals are important for your

Leica FluoCombi III™:

– M2-166-2 Leica FluoCombi III™: It describes the

specific functions of the FluoCombi III™

– M2-160-4 for MZ16 FA and M2-160-5 for MZ16 F

describe the functions of the motorized and

the manual version

– M2-216-1 describes the lamp housing and

its functions

– M2-105-0 Leica M Stereomicroscopes: It

contains all the detailed information about the

use of the stereomicroscope, the optical data

with stereo objectives, and the safety and care

instructions

– M1-267-1 Leica motor focus system: describes

the motorized focusing

Read the user manuals listed

above before the startup procedure.

In particular, please follow the safety

instructions.

To maintain the unit in its original condition and to

ensure safe operation, the user must follow the

instructions and warnings contained in these user

manuals.

1.1.1 Diagrams

Pages 3 and 4 contain figures showing the

Leica FluoCombi III™ with the function elements.

Numbers in parentheses within the descriptions

refer to the number of the figure and the items in

the figure.

Example (1.4): Figure 1is located on page 3, and

the item 4is the insert for dichroic mirrors.

1.2 Description

Leica FluoCombi III™ is an accessory for the Leica

MZ16 F, MZ16 FA fluorescence stereomicroscopes

and allows for quickly switching between a stereo

objective and an HR (High Resolution) micro

objective. In stereo mode, the 1×plan and planapo

objectives offer generous viewing fields, large

working distances and an excellent depth of field

for manipulating and dissecting.

Micro mode allows for exactly identifying the

finest fluorescent features at a microscopic reso-

lution of 1500 pairs of lines/mm. The zoom is also

effective in micro mode so that the magnification

measures 460×using the 5×HR objective. Using

16×eyepieces results in a maximum magnification

of 736×. The comfortable binocular observation is

ensured in stereo and micro mode.

MZ16 F and MZ16 FA are high-performance stereo-

microscopes for fluorescence applications. The

patented, separate TripleBeam™ light path for

fluorescence illumination and the patented

FLUOIII™ filter system provide highest-quality

fluorescence images. Every existing instrument

can be fitted with the Leica FluoCombi III™ in a

few simple steps.

2.1 Expansion diagram

Leica FluoCombi III™ – Assembly

2. Assembly

10 445 631

10 445 367 HL

10 446 352 HL RCTM

10 445 363

10 445 387

10 445 822

10 446 229

Filter

10 447 284

10 447 285

10 447 286

10 447 287

10 447 288

10 447 289

10 446 271

10 447 185 500mm

Motor focus

10 447 041 500mm

10 447 160

10 445 301

10 445 302

10 445 303

MZ16 F

10 447 064

106z

11 504 066

11 504 069

FluoCombi IIITM

10 447 324

MZ16 FA

10 447 063

10 447 243

5x HR

10 447 338

10 447 085 20x HR

Optem / Mitutoyo

10 447 290

10 447 291

10 447 292

10 447 293

10 447 294

10 447 295

10 445 819

10 447 157

Plan/Planapo

8Leica FluoCombi III™ – Assembly

2.2 Leica FluoCombi III™ standard delivery

– Leica FluoCombi III™ with microscope carrier

(Fig. 3) for the MZ16 F and MZ16 FA optics car-

rier, mechanism for swinging into the stereo or

micro mode, fine focusing with mount thread

for an HR objective and for a 1× plan or planapo

objective

– UV protection screen (7.11)

– Focusing stop with stops (7.3)

– Column adapter (7.2a)

2.2.1 Additional components

– HR planapo objective 5×/0.5 LWD (Fig. 4),

FAA 19mm or

– Adapter (5.1) for Mitutoyo/Optem objectives and

screw-in adapter (5.2) as well as the

corresponding objective

– Fluorescence stereomicroscope (7.5) with third

TripleBeam™ beam path, FLUOIII™ filter sys-

tem, rapid filter changer and Leica lamp hous-

ing for mercury high-pressure lamps as well as

binocular tube, eyepieces, stand and focusing

drive

–1×Plan or planapo objective (7.9)

– Dichroic mirror of your choice (Fig. 6)

Assembly and setup of Leica MZ16 F, MZ16 FA

fluorescence stereomicroscope and the fluores-

cence illumination are described in the respective

operating instructions (see p. 6).

Binocular tube, eyepieces, stands, mercury vapor

lamp, etc. are described in the operating instruc-

tions M2-105-0, M2-160-5, M2-160-4, M2-216-1.

Fig. 6 Dichroic mirror

Fig. 5 Adapter (1) for Mitutoyo/Optem objectives

and screw-in adapter (2)

Fig. 4 HR planapo objective 5×/0.5 LWD

Fig. 3 Leica FluoCombi IIITM

9Plan or planapo objective 1×

10 HR planapo objective 5×(or adapter and

Mitutoyo/Optem objective)

11 UV protection screen

12 Filter sets upon demand in the filter

changer and analogous with a dichroic filter

in the FluoCombi III™

13 Photo equipment (if available)

14 Gliding stage or thermal stage (if available)

2.3 Assembly sequence

1Base of stand

2Focusing drive with column (manual or

motorized) and column adapter (2a)

3Focusing stop

4Leica FluoCombi III™

5Optics carrier

6Binocular or video/photo tube and eyepieces

7Lamp housing with high-pressure lamp and

stray-light protection

8Supply unit

Leica FluoCombi III™ – Assembly

Fig. 7 Leica MZ16 F with Leica FluoCombi III™

Leica FluoCombi III™ – Assembly

2.5 Focusing stop

The focusing stop is used for safety

purposes and must always be correctly set with

respect to the micro objective.

Fasten the focusing stop (8.2) to the column

with the clamping screw (8.3).

Set the focusing stop at a height so that

the micro objective can never hit the stand or

table surface or the object during focusing and

swinging. Working distance 5×HR objective:

19mm, 20×HR objective 13mm.

With a correctly set focusing stop, the two

stops prevent focusing too low, either manually or

motorized.

If the focusing stop is set too low

– the objective could be damaged.

– the object could be crushed or damaged.

– if the lowest focus position is approached too

quickly using the motor focus, the fingers of the

operator could be pinched between objective

and object.

Fig. 9 Leica FluoCombi III™ from below

1Transport safety screws

Fig. 8

1Column adapter

2Focusing stop

3Clamping screw for fastening

the focusing stop

2.4 Column with focusing drive ➜base of

stand

First, attach the column adapter between stand

column and base. It is used to ensure that the

center of the objective lies exactly over the center

of the stage.

Fasten the column adapter (8.1) with the

3 hexagon-head screws on the column.

Fasten the column with the column adapter on

the stand base according to the operating

instructions M2-105-0.

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