Motic B1-220A Bedienungsanleitung

Instruction Manual

B1 Series

Motic Microscopes, European Division

1. E

ieces 13. Sliding

interpupilliar

adjustment.

2. Interpupillary

Scale

3. Revolving

Nosepiece

4.Ob

ectives

5. Moveable fin

6. Specimen holder

mechanism

7. Slide holder

8. Sta

10. Condenser

9. Diaphragm

15. Head

17. Arm

14. Eyepiece tube with

diopter correctors

18. Knurled locking

screws for securing

specimen holder

19. Coarse Focus

Knob

20. Fine Focus

Knob

21. Knobs

controlling

16. Knurled locking

screw

stage movemen

11. Filter holder

22. Illuminato

12. Base 23. Ligh

intensity control

B1-220A

-2-

Introduction

Thank you for your purchase of a Motic microscope. Motic microscopes are precision instruments, subjected

to meticulous examination in order to reach you in perfect condition. Their design combines easy

management and optimum functioning with minimum maintenance.

The information contained in this manual is likely to go beyond what the average user needs to know to use

the microscope, however, it is provided to answer any queries that may arise.

Your new microscope combines high performance features, with an excellent degree of optical resolution

and clarity of image. It incorporates a mechanical stage that provides a travel range of 70mm x 50mm in X

and Y directions with a graduation of up to 0.1 mm, thus permitting the perfect positioning of the specimen.

Also included are objectives located on a ball bearing nosepiece allowing movement in both directions; a

precision coarse and fine focusing system; a moveable Abbe condenser with a numerical aperture of 1.25

N.A. and a built-in 12V/ 20W halogen variable light source.

These instructions should be read carefully before operating the microscope. They will permit you to use

your new microscope to its fullest capabilities. Terminology used to describe components and controls can

be found in the diagram on page 2.

These instructions are based on the assembly and use of the B1-220 model (Binocular) with

additional notes applying specifically to other models in the series.

Unpacking

All components of the microscope have been carefully packed to make sure they reach you in perfect

condition. We recommend that you do not discard any packing containers in case you need to return the

microscope, store it for long periods of time; or should it become necessary to transport it to a technical

service for any repair, or maintenance procedure.

The box should contain the following components, depending on the model:

• B1-211 (Monocular): Stand, Power cable, monocular head, eyepiece, eyepiece protector, four

objectives, condenser, sample holder mechanism, blue, green and yellow filter, fuse, dust cover, a

bottle of immersion oil and a 2mm hexagonal key.

• B1-220 (Binocular): Stand, Power cable, binocular head, two eyepieces, two eyepiece protectors,

four objectives, condenser, sample holder mechanism, blue, green and yellow filter, fuse, dust cover,

a bottle of immersion oil and a 2mm hexagonal key.

• B1-223 (Trinocular): Stand, Power cable, trinocular head, two eyepieces, two eyepiece protectors,

four objectives, condenser, sample holder mechanism, blue, green and yellow filter, fuse, dust cover,

a bottle of immersion oil and a 2mm hexagonal key.

Remove, and handle the microscope and all its components with extreme care.

Avoid touching the lenses of the optical elements and keep clear of contact with dust, water or other

contaminating agents, as they could stain, or damage the lens surface and affect the quality of the

image.

A. Place the microscope in an upright position on a flat, stable and clean surface.

B. Unscrew the black plastic protectors from the revolving nosepiece (3).

C. Remove the rest of the components from the box.

-3-

Assembly

All the steps described for the assembly of the microscope must be undertaken with extreme care,

and without forcing the placement of the distinct parts and elements of the microscope.

A. Condenser (10): Turn the coarse

focus knob (19) until the stage (8)

reaches its highest position. Turn

the focussing knob on the

condenser (Fig.1) until the

condenser holder reaches its

lowest position. Unscrew the

knurled locking screw on the

condenser, and insert the

condenser in the mounting ring so

that the aperture of the diaphragm

(9) faces the front of the

microscope and so can be

adjusted easily. Once firmly

placed in the mounting ring, firmly

tighten the locking screw, and turn

the condenser focusing knob until

in its highest position.

Condense

set screw

Condenser

focusing

knob

Fig. 1

-4-

B. Specimen Holder Mechanism (6): Turn the Coarse Focus Knob (20) until the stage (8) reaches its

lowest position. Unscrew the two knurled locking screws (18) of the specimen holder mechanism.

Place the mechanism on the stage with the moveable finger lever facing upwards (5) so that the

holes of the mechanism line up with the locking screws. Firmly screw the screws back into place.

C. Objectives (4): Ensure that the revolving nosepiece (3) is well positioned: i.e. that it does not revolve

freely, but rotates until a “click” is heard, showing that it is secure in place. Place objectives, starting

with the lowest magnification 4X in any of the holes in the revolving nosepiece. Next, place that of

the second lowest magnification 10X in the hole alongside, and so forth: the 40X objective, and

finally the 100X objective can be placed.

D. Head (15): Loosen the knurled locking screw on the head and remove the protective cover on the

collar where the head is placed. Insert the head, until it is well supported on the stand (17). Although

the head can be placed in any position, we recommend for your comfort that the eyepiece tubes (14)

face forward on the microscope. Firmly re-tighten the knurled locking screw.

E. Eyepieces (1): Remove the protective covers on the eyepiece tubes (14). Gently place the eyepieces

in the tubes without touching the lens surface.

• B1-211 and B1-212: Remove the

protective cover on the eyepiece tube.

Using a jewellers screwdriver, loosen the

tiny screw situated on the lateral of the

tube. Gently, and without touching the

surface of the lens, place the eyepiece in

the tube. Re-tighten the locking screw on

the eyepiece.

F. Filter: Swing out the filter holder horizontally

and place the blue filter in the filter holder

(11) located under the condenser (10). Make

sure that the filter is well positioned.

G. Power cable: Insert the power cable in the

connector at the lower back of the

microscope. (Fig. 1) On switch Fuse

Fig. 2

Warning: Before connecting the microscope to a

power source, check that the voltage coincides with

that on the microscope.

Operation

A. Starting up.

1. Before using the microscope, adjust the light intensity control (23) to minimum position. This

should be repeated every time the microscope is switched on or off to prolong the use of the

bulb.

2. Press switch to ON position. (Fig. 2)

3. Turn illumination control until the image is illuminated.

4. Light intensity must be adjusted according to the objective used, or the type of preparation to

be observed.

-5-

B. Interpupillary adjustment of head. (Only for models B1-220 and B1-223)

1. Look through the eyepiece and adjust the distance between the eyepiece tubes (14) by

grasping the head, by its four gripped corners (13), and moving it horizontally.

2. When a full field of view is observed through both lenses, and images blend into one, the

interpupillary distance is correct for the user’s eyes. Take note of the index reading marked

in the interpupillary scale (2) for the following step.

3. Adjust the diopter scales (14) situated on both eyepiece tubes until the reading of both

scales coincides with the value of the interpupillary distance scale. This step is necessary to

maintain the parfocality of the objectives.

4. Readjust the diopter scales for variation in interpupillary distance.

C. Focusing the microscope.

1. Turn the revolving nosepiece (3) and place the 4X objective (4) in the optical path ensuring

that it is properly clicked into place.

2. Turn the coarse focus knob (19) until the stage (8) is in its lowest position.

3. Place a microscopic sample on the stage, make sure the cover slip faces upwards.

• Swing the moveable finger (5) on the mechanism (6), place specimen against fixed side

of slide holder (7) and gently release the moveable finger until the specimen is

supported in place.

4. Make sure that the specimen is in the optical path by adjusting the stage using the knobs

controlling the X/Y movements (21).

5. Looking through the eyepiece, turn the coarse focus knob until the preparation appears in

focus.

6. Readjust the focus with the fine focus knob (20) until the image appears sharply defined.

D. Adjust diopter for differences in eyesight. (Only for models B1-220 and B1-223)

1. With your right eye, look into the right eyepiece tube (1) and adjust the sharpness of the

image using the fine focus knob (20).

2. With your left eye, looking through the left eyepiece, adjust the focus by rotating the diopter

corrector (14) on the left hand eyepiece tube until the image is sharp. Do not use the fine

focus knob.

E. Adjusting the aperture of the diaphragm.

The aperture of the diaphragm (9) should not be used to control the brightness of the light.

Diaphragms are designed to obtain high resolution of the sample, and provide contrast in the

image. Smaller apertures will deliver higher contrast to image. However, closing the aperture

too much will reduce resolution. Experimentation is the best method of determining the

correct aperture of the diaphragm. Suggested apertures for each objective are as follows:

OBJECTIVE APERTURE OF IRIS

4X From fully closed to 1/8 open.

10X From 1/8 to 1/4

40X From 1/4 to 1/2

100X From 1/2 to 3/4

-6-

F. Changing magnification.

1. Position the 10X objective (4) in the optical path.

2. This microscope has been parfocalized, although it is possible that small differences exist

between objectives. If so, adjust focus slightly using the fine focus knob (20).

3. When changing to the 40X and 100X objectives, take care that objectives do not touch the

sample, to prevent possible damage to the front lens of the objectives.

4. In order to obtain maximum resolution of the 100X oil immersion lens, it is necessary to

apply immersion oil between the cover glass of the slide, and the front lens of the objective.

a. Use a very small amount of immersion oil, a tiny drop should be enough.

b. If air bubbles appear, they can be removed by slightly moving the revolving

nosepiece (3) and repositioning it.

c. After viewing all parts which have come into contact with the immersion oil must

be cleaned. For cleaning, use a soft cotton cloth, lightly dampened with Xylene.

Failure to clean the 100X objective could result in the oil drying on the lens

surface, thus blocking view when the objective is used again, and possibly

damaging the lens.

NB: Immersion oil must ONLY be used with the 100X objective, which is the

only objective prepared for it. If any other objective comes into contact with

immersion oil, it must be cleaned immediately.

G. Critical illumination.

The ideal level of illumination is when all illumination elements are brought into proportion,

basically, by the condenser (10). To achieve critical illumination an object over the illuminator

(22) must be in focus.

1. Focus on a sample with the 10X objective (4).

2. Place a flat object on the illuminator which permits light to pass through, a slide for

example.

3. Without letting go of the slide, focus, using the collar to move the condenser (Fig.1).

4. When critical illumination is achieved, the slide can be removed. If any irregularity

appears in the field of view, i.e. an optical element from the focussed illuminator,

move the condenser just enough to take it out of focus, thus obtaining the best level

of illumination possible, and closest to critical illumination.

How to adapt a photographic, or a video camera. (Only for model B1-223)

Model B1-223 comes equipped with a vertical port on the top of the head which permits the attachment of a

photographic, or reflex type camera through use of the corresponding adapters. (Fig. 3)

The three position sliding rod (Fig. 4) allows the microscope image to appear easily through this third path.

-7-

• When the rod is pushed completely into

head, 100% of the microscope image is

directed to the binocular eyepiece for

observation.

• In the mid-position, adjusted until a click is

heard, 100% of the image is directed to the

trinocular port for photographs or video

images.

• In the fully extended position, 30% of the

image is directed to the binocular eyepieces,

and 70 % to the trinocular port.

A. To adapt a photographic camera, an adapter tube is required. (Fig. 3). This adapter tube includes a

2.5X photographic lens which measures the parfocality correctly between the images of the binocular

and vertical ports. The adapter tube also requires a T mount adapted to fit the T threads of all reflex

photographic camera brands on the market.

1. To install the camera on the microscope, first remove the front lens of the camera and put the

thread in its place. Then screw in the adapter tube to the thread of the camera.

2. Loosen the knurled screw located on the side of the dual viewing head until the protective cap

that covers the port can be removed (Fig.4).

Adapter tube

Fig. 3

Photo adapter

Video adapter

Light

controlling

rod

Knurled

screw

3. Insert the adapter tube with the camera

mounted on the vertical port. If it does

not connect easily, unscrew the knurled

screw until the adapter tube drops into

the port and is firmly in place.

4. Tighten the knurled screw firmly to

support the camera well in place.

5. Place the sliding rod to mid, or

extended position to allow the image to

be directed to the camera.

6. Operate the camera according to the

manufacturers instructions.

. 4

B. To adapt a video camera, an adapter tube is required (Fig. 3). This adapter contains a 0,5X lens for

video that measures the correct parfocality between binocular, and the vertical port images. The

vertical port is responsible for the images appearing on the TV monitor. The adapter tube also

includes a “C” knurled ring and a “CS” type ring to accommodate different types of video cameras.

1. To install the camera on the microscope, screw the adapter tube on the video camera.

2. Loosen the knurled screw located on the side of the dual viewing head until the protective cap

that covers the port can be removed (Fig.4).

-8-

3. Insert the adapter tube with the camera mounted in the vertical port. If it does not fit easily

unscrew the knurled screw until the adapter tube slots into place firmly.

4. Tighten the knurled screw firmly so the camera is well supported.

5. Place the sliding rod to mid, or extended position, to allow the image to be directed to the

camera.

6. Operate the camera following the instructions of the manufacturer.

7. If the image on the TV monitor does not stay in focus when the objective is changed, it is

possible that the CS ring is responsible for it. It may be necessary to either replace or remove it

according to the procedure to obtain parfocality.

Maintenance

WARNING: FOR YOUR SECURITY, SWITCH OFF AND REMOVE PLUG FROM POWER SOURCE

OUTLET BEFORE MAINTAINING YOUR MICROSCOPE IN ORDER TO AVOID SHOCK OR FIRE

HAZARD.

CONSULT YOUR DISTRIBUTOR IF YOUR MICROSCOPE REQUIRES ANY MAINTENANCE OR REPAIR

PROCEDURE NOT COVERED IN THIS INSTRUCTION MANUAL.

A. Optical maintenance.

1. Do not attempt to disassemble any lens component.

2. Before cleaning any lens, remove surface dust with a fine brush especially for lenses.

Alternatively, you can use low pressure compressed air, available in any photography shop.

3. Cleaning Eyepiece

a. Do not remove eyepiece (1) from eyepiece tube (3).

b. Only clean the external surface, misting the lens with breath.

c. Afterwards, dry the lens with special lens paper using circular movements from the

centre of the lens to the outer part. Do not clean lenses when already dry, as they can

be scratched easily.

4. Cleaning the objectives.

a. Do not remove the objectives (4) from the microscope.

b. Only clean the surface area. Use a soft cotton cloth dampened with Xylene. Afterwards,

dry the lens with the same cloth.

5. Cleaning the condenser.

a. Only clean the top lens surface. (10) Use same procedure as used for eyepiece or

objective lens..

6. Cleaning the lens of the illuminator.

a. Only clean the top lens of the illuminator (22). Use the same procedure as used for the

eyepiece or objective lenses.

-9-

B. Electrical maintenance.

WARNING: FOR YOUR SECURITY, SWITCH OFF AND REMOVE PLUG FROM POWER

SOURCE OUTLET BEFORE MAINTAINING YOUR MICROSCOPE IN ORDER TO AVOID SHOCK

OR FIRE HAZARD.

1. Changing the bulb.

a. Lay the microscope on its side, taking

extreme care especially with the eyepieces

(1) and the specimen slide holder (6).

b. Unscrew the screw pointed out by the

arrow. (Fig. 5).

c. Open the cover to give access to the bulb.

d. With a cloth, carefully grasp the bulb and

gently pull, to disconnect it from its socket.

e. Do not touch the replacement bulb with

fingers. Use a clean cloth and insert the

pins of the bulb into socket.

f. If the bulb is touched accidentally, clean it ,

as finger contact may affect light

transmission, and the life span of the bulb.

Cap of

access Fig. 5

g. Close hinged door and tighten locking screw.

2. Changing the fuse.

a. Using a flat screwdriver, gently press on the slot located on the fuse cap (Fig. 1) and

make a ¼ turn in the direction indicated by the arrow.

b. Release pressure on the loosened fuse cap, and remove completely.

c. Remove the fuse located on the cap, by pulling it out, and insert new fuse. Ensure that a

0.5 Amp. fuse is used.

d. Replace the fuse cap.

e. Repeat step (a), this time making a ¼ turn in the opposite direction to that indicated by

the arrow. The cap must be closed securely.

C. Mechanical maintenance.

1. Adjusting the tension of the coarse focus control.

The tension adjustment control for the coarse focus knob (Fig.5) is located between the coarse

focus knob (19) and the arm (17). The coarse focus knob tension is pre-adjusted by the

manufacturer. The ideal tension point is that which permits coarse focus knob to move as loosely

as possible, without the stage (8) moving down on its own.

-10-

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