Nikon ECLIPSE Ni-E Bedienungsanleitung

Operation

Flowchart

Before

Microscopy

Components

Safety Precautions

Notes on Handling

the Product

Individual

Operations

Epi-fluorescence

Microscopy

IR-DIC

Microscopy

Upright Motorized Microscope

(Focusing Nosepiece System)

Instruction Manual

Operation

M572 E 11.8.NF.1 (1/2)

*M572EN01*

Introduction

Thank you for purchasing a Nikon product.

This instruction manual is written for users of the Nikon ECLIPSE Ni-E (Focusing Nosepiece System) microscope. To

ensure correct usage, read this manual carefully before operating this product.

●No part of this manual may be reproduced or transmitted in any form without prior written permission from Nikon.

●The contents of this manual are subject to change without notice.

●The equipment described in this manual may differ from the actual product in its appearance.

●Although every effort has been made to ensure the accuracy of this manual, errors or inconsistencies may remain. If

you note any points that are unclear or incorrect, please contact your nearest Nikon representative.

●Some of the equipment described in this manual may not be included in the set you have purchased.

●If you intend to use any other equipment with this product, read the manual for that equipment too.

●If this equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may

be impaired.

●Training: This product can be used without special training, provided that this manual is read thoroughly before use. Kindly

contact your nearest Nikon representative if you have any questions, find any errors, or wish to provide us with your

opinion.

Introduction

Contents of the Manual

The instruction manual for ECLIPSE Ni-E (Focusing Nosepiece System) is provided in two volumes.

Operation (This manual)

Safety Precautions

Components

Microscopy Operations

Before Microscopy

Operation Flowchart

IR-DIC (Infrared Ray Differential Interference

Contrast) Microscopy Procedure

Epi-fluorescence Microscopy Procedure

Individual Operations

Assembly/Maintenance

Assembly

Troubleshooting

Maintenance and Storage

Specifications

Before reading the “Assembly/Maintenance” manual,

read the “Safety Precautions” in the “Operation”

manual.

Symbols Used in This Manual

The following symbols are used in this manual.

Symbols for Safety

WARNING

CAUTION

Highlights important information that should be noted for safety. Read “Safety Precautions” for details.

Other Symbols

Indicates information you should note or comply with to prevent defects or malfunction of this product.

Indicates information you should be aware of in using this product, as well as other useful information.

Summary of Contents

iii

Operation

Flowchart

Before

Microscopy

Components

Safety Precautions

Notes on Handling

the Product

Individual

Operations

Epi-fluorescence

Microscopy

IR-DIC

Microscopy

Summary of Contents (See the next page for detailed contents.)

Introduction

Contents of the Manual

Symbols Used in This Manual

Safety Precautions

Notes on Handling the Product

Components

(System Configuration and Controls)

Before Microscopy

(Information Setting for Motorized Operation)

Operation Flowchart

IR-DIC (Infrared Ray Differential

Interference Contrast) Microscopy

Procedure

Epi-fluorescence Microscopy

Individual Operations

(Display Panel, Operation Buttons, Instructions for Each Part/

Device, Image Capture)

Contents

Introduction .........................................................................................................................i

Contents of the Manual ....................................................................................................ii

Symbols Used in This Manual ..........................................................................................ii

Summary of Contents ....................................................................................................... iii

Safety Precautions............................................................................................................ vi

WARNING and CAUTION Symbols ................................................................................vi

Meaning of Symbols Used on the Product......................................................................vi

WARNING ................................................................................................................vii

CAUTION ..................................................................................................................ix

Notes on Handling the Product......................................................................................... xi

Components............................................................................................. 1

1 Ni-E Focusing Nosepiece System Basic Configuration ............................................2

1.1 Components ....................................................................................................... 2

1.2 Controls .............................................................................................................. 3

1.3 Operation Buttons .............................................................................................. 4

1.4 Connectors ......................................................................................................... 5

Microscopy Operations........................................................................... 7

1 Before Microscopy ....................................................................................................8

2 Operation Flowchart................................................................................................10

3 IR-DIC (Infrared Ray Differential Interference Contrast) Microscopy Procedure ....12

4 Epi-fluorescence Microscopy Procedure.................................................................29

Individual Operations ............................................................................ 41

1 Display Panel Details ..............................................................................................42

2 Using Operation Buttons on Ni-E ............................................................................45

2.1 Front Operation Button..................................................................................... 45

2.2 Right Operation Button..................................................................................... 46

2.3 Left Operation Button ....................................................................................... 47

3 Adjusting the Brightness of a Diascopic Image.......................................................48

3.1 Adjustment by Lamp Voltage ........................................................................... 48

3.2 Adjustment with ND Filters............................................................................... 49

4 Focusing on the Sample (Vertical Objective Movement) ........................................50

4.1 Proper Focusing Procedure ............................................................................. 50

4.2 Focus Knobs on the Main Body ....................................................................... 51

4.3 Resetting the Z-axis Coordinate....................................................................... 52

4.4 Refocusing ....................................................................................................... 53

5 Bringing the Target into the Optical Path (Horizontal Stage Movement) .................54

5.1 Standard Stage Operation................................................................................ 54

5.2 Operating the Motorized XY Stage................................................................... 55

6 Adjusting the Diopter...............................................................................................56

7 Focusing and Centering the Condenser .................................................................57

8 Adjusting the Aperture Diaphragm ..........................................................................58

9 Using the Condenser ..............................................................................................59

Contents

10 Adjusting the Field Diaphragm ................................................................................61

11 Operating the Nosepiece (objective operation).......................................................62

11.1 Objective Vertical Movement Lever (objective switching, dipping) .................. 62

11.2 Parfocal Correction of Objective ...................................................................... 63

11.3 Centering the Objective.................................................................................... 64

11.4 Inserting/Removing the DIC Slider................................................................... 64

11.5 Single nosepiece .............................................................................................. 64

12 Using the Polarizer Turret .......................................................................................65

13 Switching the Optical Path of the Tube ...................................................................65

14 Adjusting the Binocular Section ..............................................................................66

15 Water Immersion .....................................................................................................66

16 Differential Interference Contrast Microscopy .........................................................67

16.1 Tips for Differential Interference Contrast Microscopy..................................... 67

16.2 Using Optical Elements .................................................................................... 68

17 Epi-fluorescence Microscopy ..................................................................................69

17.1 Epi-fluorescence Cube Turret Operation (Switching Excitation Methods)....... 69

17.2 Selecting Filters................................................................................................ 71

17.3 Protecting the Sample and Preventing It from Decoloration

(Using the Shutter) ........................................................................................... 73

17.4 Adjusting the Brightness of the Fluorescent Image

(Using ND Filters and the Aperture Diaphragm) .............................................. 75

17.5 Restricting the Illumination to the Area of the Sample Being Viewed

(Centering and Adjusting the Field Diaphragm)............................................... 76

17.6 Changing the Waveform Characteristics of the Excitation Light

(D-FB Excitation Balancer)............................................................................... 77

17.7 Other Notes on Epi-fluorescence Microscopy.................................................. 78

18 Useful Functions .....................................................................................................79

18.1 Mode Function.................................................................................................. 79

18.2 Interlocking Function ........................................................................................ 82

19 Capturing Images....................................................................................................83

20 Operation on DS-L3 ................................................................................................87

20.1 Setting Up the Microscope ............................................................................... 87

20.2 Microscope Control ........................................................................................ 100

Safety Precautions

Notes on Handling

the Product

Safety Precautions

To ensure correct and safe operation, read this manual before using this product.

WARNING and CAUTION Symbols

Although this product is designed and manufactured to be completely safe during use, incorrect usage or failure to follow

the safety instructions provided may cause personal injury or property damage. To ensure correct usage, read this manual

carefully before using this product. Do not discard this manual and keep it handy for easy reference.

Safety instructions in this manual are marked with the following symbols to indicate their importance. For your safety,

always follow the instructions marked with these symbols.

Symbol Description

WARNING Disregarding instructions marked with this symbol may lead to serious injury or death.

CAUTION Disregarding instructions marked with this symbol may lead to injury or property damage.

Meaning of Symbols Used on the Product

When appearing on this product, the symbols below indicate the need for caution at all times during use. Read the

relevant instructions in this manual before attempting to use or adjust any part to which the symbol has been affixed.

Biohazard

This symbol is affixed to the front of the stand of this product, to call your attention to the following:

●WARNING: Using this product may constitute a biohazard risk if a sample comes into contact with this

product.

●To avoid biohazard contamination, do not touch the contaminated portion with bare hands.

●Decontaminate the contaminated part in accordance with the standard procedure specified for your

laboratory.

Precautions against heat

This symbol is affixed to the lamphouse to call your attention to the following:

●During and immediately after a period of illumination, the lamp and surrounding areas (including the

lamphouse) are very hot.

●Risk of burns. Do not touch the lamp or surrounding areas during or immediately after a period of

illumination.

●Make sure the lamp and surrounding areas have cooled sufficiently (which may take approx. 30

minutes) before attempting to replace the lamp.

Precautions against heat

This symbol is affixed to the top of the arm (both standard and contact), but “Precautions against heat” is

not required when using in combination with Ni-E Microscope.

Safety Precautions

vii

Safety Precautions

Notes on Handling

the Product

WARNING

1 Do not disassemble.

Disassembling this product may result in electric

shock or malfunction. Malfunction and damage

due to disassembling or modification are

unwarranted.

Do not disassemble parts other than those

described in this manual. If you experience

problems with this product, contact your nearest

Nikon representative.

2 Read the instruction manuals carefully.

To ensure safety, thoroughly read this manual and

the manuals for other equipment to be used with

this product. Particularly, all warnings and

cautions given at the beginning of each manual

must be observed.

Safety is a top design priority for Nikon products.

Safety is ensured as long as the user observes all

of the warnings and cautions given in the

manuals, and uses the system only for its

intended purpose. However, failure to heed the

warnings and cautions given in the manuals,

subjecting the system to shock or impact, or

attempting to disassemble the system may result

in unexpected accidents and injury.

Product with NI-FLEI epi-fluorescence

attachment:

The light source used for Epi-fluorescence

microscopy (HG Precentered Fiber Illuminator)

requires special care during handling because of

its characteristics. Be sure to refer to the manual

for the light source being used.

3 Notes on the power cord

Be sure to use the specified power cord. Use of

other power cords may result in malfunction or

fire. This product is classified as having Class I

protection against electric shock. Make sure this

product is connected to an appropriate protective

earth terminal.

See Chapter 4 “2. Performance Properties” in the

“Assembly/Maintenance” instruction manual for

designated power cords.

●To prevent electric shock, always turn off the

power switch (press to the “O” position) for the

microscope before connecting or disconnecting

the power cord.

4 Heat from the illuminator

During and immediately after a period of

illumination, the lamp and surrounding areas

(including the lamphouse) are very hot.

●Do not touch the lamp or surrounding areas

during or immediately after a period of

illumination. There is a risk of burn if you touch

the hot area.

●Always attach the lamphouse cover when using

this product.

●Make sure the lamp and surrounding areas

have cooled sufficiently (which may take

approx. 30 minutes) before attempting to

replace the lamp.

●To avoid the risk of fire, do not place fabric,

paper or highly flammable volatile materials

such as gasoline, petroleum benzine, paint

thinner, or alcohol near the lamphouse while

the lamp is lit or for a period of approximately

thirty minutes after the lamp is turned off.

Safety Precautions

viii

Safety Precautions

Notes on Handling

the Product

WARNING

5 Hazards of mercury lamps

(when using the NI-FLEI Epi-fluorescence

attachment)

The light source used with the epi-fluorescence

attachment (HG Precentered Fiber Illuminator)

requires special care during handling because of

its characteristics. For safe and correct use of this

system, carefully read the warnings below. Keep

in mind all potential hazards. Additionally, carefully

read the manual for the illuminator and the manual

from the lamp manufacturer (if provided), then

follow the instructions given therein. Failure to

heed the warnings and cautions given in the

manuals, subjecting the system to shock or

impact, or attempting to disassemble the system

may result in unexpected accidents and injury.

●Ultraviolet light

When lit, mercury lamps radiate ultraviolet light

that can damage the eyes and skin. Direct

viewing of the light may result in blindness.

When changing filter cubes, always turn off the

light source of the Epi-fluorescence

attachment. Leaving the lamp turned on during

filter cube replacement may result in ultraviolet

exposure.

Leave the D/UV slider of the D-ES EPI ND

slider within the optical path. Removing the

D/UV slider from the optical path will cause

your eyes to become exposed to ultraviolet

light.

●High-pressure gas

The lamps contain sealed gas under very high

pressure. And the pressure increases when the

lamp is on. If the lamp is scratched,

contaminated, subjected to high external

pressure or physical impact, or used beyond its

service life, the sealed gas may leak or the

lamp may burst, resulting in gas inhalation,

injury from glass, or other accidents.

●Heat

When the lamp is lit, the lamp and surroundings

will become extremely hot. Do not touch the

lamp with bare hands or place flammable

materials near the lamp. Failure to comply may

result in burns or fire.

●Designated lamp

Be sure to use the designated lamp. Using

other types of lamps may result in accidents,

including bursting of the lamp.

6 Hazardous sample handling

This product is intended primarily for microscopic

observations and image capture of samples

(biological tissue, brain slice, etc.) in chambers.

Check to determine whether a sample is

hazardous before handling. If sample is

hazardous, handle it according to the standard

procedure specified for your laboratory. If the

sample is potentially infectious, wear rubber

gloves and avoid directly touching samples. If

such a sample is spilled onto this product, the

portion must be decontaminated in a safety

manner. Consult your safety supervisor or safety

standard of your facility.

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