Nikon SMZ25 Bedienungsanleitung

Research Stereo Microscope

SMZ25/SMZ18

Instruction Manual

M608EN11(1/2) 23.1.31.NW_EN

*M608EN11*

Introduction

Thank you for purchasing a Nikon product.

This instruction manual is written for users of the Nikon research stereo microscopes for SMZ25 Zooming Body/SMZ18

Zooming Body. To ensure correct usage, read this manual carefully before operating this product.

●No part of this manual may be reproduced or transmitted in any form without prior written permission from Nikon.

●The contents of this manual are subject to change without notice.

●The equipment described in this manual may differ from the actual product in its appearance.

●Although every effort has been made to ensure the accuracy of this manual, errors or inconsistencies may remain. If you

note any points that are unclear or incorrect, please contact your nearest Nikon representative.

●Some of the equipment described in this manual may not be included in the set you have purchased.

Contents

Introduction .........................................................................................................................i

Safety Precautions.............................................................................................................v

Label Attached to the Product.......................................................................................... v

WARNING and CAUTION Symbols............................................................................... vii

WARNING.................................................................................................................viii

CAUTION.................................................................................................................... x

Notes on Handling the Product ........................................................................................ xii

Components............................................................................................. 1

1 Microscopy System Using Episcopic Illumination .................................................... 2

1.1 Names of the Devices and Operation Sections..................................................2

2 Microscopy System Using Diascopic Illumination and Epi-fluorescence

Illumination ............................................................................................................... 3

2.1 Names of the Devices and Operation Sections..................................................3

2.2 Buttons on the P2-RC Remote Controller .......................................................... 4

3 Compact System ...................................................................................................... 5

3.1 Names of the Devices and Operation Sections..................................................5

Microscopy Procedures .......................................................................... 7

1 Operation Flowchart ................................................................................................. 8

2 Procedure for Microscopy under Episcopic Illumination........................................... 9

3 Procedure for Microscopy under Diascopic Illumination ........................................ 13

4 Procedure for Epi-fluorescence Microscopy........................................................... 17

Assembling ............................................................................................ 19

1 System Configuration ............................................................................................. 20

2 Device List.............................................................................................................. 22

3 Assembly Procedures ............................................................................................ 24

3.1 Assembling the EPI System or DIA + EPI-FL System......................................24

3.2 Assembly of the Compact/EPI System and Compact/DIA + EPI-FL System...42

3.3 Assembly of Other Devices (Optional) .............................................................48

Maintenance and Storage ..................................................................... 53

1 Cleaning ................................................................................................................. 54

1.1 Cleaning Lenses ...............................................................................................54

1.2 Cleaning Parts Other than the Lens .................................................................54

1.3 Disinfecting the Product....................................................................................55

2 Storage................................................................................................................... 55

3 Periodic Inspection (Charged) ................................................................................ 55

Specifications ........................................................................................ 57

1 Performance Properties ......................................................................................... 58

2 Physical Properties................................................................................................. 64

Contents

iii

Safety Principle...................................................................................... 65

1 Safety Principle ...................................................................................................... 66

1.1 Test Principle (Bright-field Observation) ...........................................................66

1.2 Intended Purpose .............................................................................................66

1.3 Intended User ...................................................................................................66

1.4 Applicable Standards........................................................................................66

Specific Operations ............................................................................... 67

1 Turning On the Power ............................................................................................ 68

2 Adjusting the Brightness......................................................................................... 70

3 Switching the Optical Path of the Tube .................................................................. 71

4 Adjusting the Angle of Depression ......................................................................... 72

5 Adjusting the Diopter .............................................................................................. 72

6 Adjusting the Interpupillary Distance ...................................................................... 73

7 Focusing on the Sample......................................................................................... 74

8 Changing the Magnification .................................................................................... 76

8.1 Image Zooming.................................................................................................76

8.2 Switching Objectives (Using the P2-RNI2 Intelligent Nosepiece) ....................77

9 Shifting a Sample ................................................................................................... 78

9.1 Sliding the Stage Plate .....................................................................................78

9.2 Shifting the Stage in the X and Y Directions (P-SXY64 XY Stage)..................78

9.3 Using the Sliding Stage ....................................................................................78

10 Using the Aperture Diaphragm ............................................................................... 79

11 Using Episcopic Illumination................................................................................... 79

12 Using Diascopic Illumination .................................................................................. 80

13 Using Epi-Fluorescence Illumination ...................................................................... 81

13.1 Switching Filter Cubes ...................................................................................... 81

13.2 Selecting a Filter Cube .....................................................................................82

13.3 Important Notes for Epi-Fluorescence Microscopy ..........................................82

14 Remote Operation .................................................................................................. 82

15 Capturing Images ................................................................................................... 83

15.1 Procedure for Capturing Images ......................................................................83

15.2 Tips on Microscope Settings for Photomicroscopy ..........................................84

16 Internally Saved Information................................................................................... 85

Functions and Operations of the Devices...........................................87

1 Zooming Bodies ..................................................................................................... 88

1.1 SMZ18 Zooming Body ...................................................................................... 88

1.2 SMZ25 Zooming Body ...................................................................................... 89

2 Focus Units, Focus Mount...................................................................................... 92

2.1 P2-FU Focus Unit .............................................................................................92

2.2 P2-MFU Motorized Focus Unit .........................................................................93

2.3 P2-FMDN Focus Mount....................................................................................96

3 Nosepiece, Focus Mount Adapter .......................................................................... 98

3.1 P2-RNI2 Intelligent Nosepiece .........................................................................98

3.2 P2-FM Focus Mount Adapter............................................................................98

4 Objectives............................................................................................................... 99

Contents

5 Bases, Stands, and Stages .................................................................................. 100

5.1 P2-PB Plain Base ...........................................................................................100

5.2 P2-DBL LED Diascopic Illumination Base ...................................................... 101

5.3 P2-DBF Fiber Diascopic Illumination Base ....................................................101

5.4 P-PS32 Plain Stand ........................................................................................ 102

5.5 P-DSL32 LED Diascopic Illumination Stand...................................................103

5.6 P-DSF32 Fiber Diascopic Illumination Stand .................................................103

5.7 P-SXY64 XY Stage .........................................................................................103

5.8 C-SSL DIA Sliding Stage ................................................................................104

5.9 C-TRS Tilting Stage ........................................................................................104

6 Tubes.................................................................................................................... 105

6.1 P2-TERG 100/ P2-TERG 50 Trinocular Tilting Tube......................................105

6.2 P2-TL100 Trinocular Tube L ...........................................................................105

7 Episcopic Illuminator ............................................................................................ 106

7.1 P2-CIA Coaxial Epi Illuminator .......................................................................106

7.2 C-FDF Flexible Double Arm Fiber Illumination Unit........................................107

7.3 P2-FIR Ring Fiber Illumination Unit ................................................................107

7.4 P2-FIRL2 LED Ring Illumination Unit 2 ..........................................................107

8 Diascopic Illuminator ............................................................................................ 108

8.1 P2-DBL LED Diascopic Illumination Base ...................................................... 108

8.2 P2-DBF Fiber Diascopic Illumination Base .................................................... 110

8.3 P-DSL32 LED Diascopic Illumination Stand................................................... 112

8.4 P-DSF32 Fiber Diascopic Illumination Stand ................................................. 115

8.5 P-DF2 LED Dark Field Unit 2 ......................................................................... 117

8.6 P2-POL Simple Polarizing Attachment ........................................................... 117

9 Epi-Fluorescence Attachment ...............................................................................118

9.1 P2-EFLI Epi Fluorescence Attachment .......................................................... 118

9.2 P2-EFLM Motorized Epi Fluorescence Attachment........................................120

10 Remote Control Devices ...................................................................................... 122

10.1 P2-RC Remote Controller............................................................................... 122

10.2 AZ-FSW Foot Switch ......................................................................................124

10.3 AZ-PCR Photo Release..................................................................................126

11 Control/Communication Devices and Relay Devices ........................................... 127

11.1 P2-CTLB Control Box .....................................................................................127

11.2 P2-CTLA Control Box .....................................................................................129

11.3 P2-RLY Relay Box ..........................................................................................130

11.4 P2-RLYC Relay Cable ....................................................................................130

Troubleshooting................................................................................... 131

1 Image Viewing...................................................................................................... 132

2 Operation.............................................................................................................. 132

3 Motorized Devices................................................................................................ 133

Safety Precautions

•To ensure correct usage, read this instruction manual carefully before using the equipment.

Read also the instruction manuals if separately supplied with the components of the equipment.

•Be sure to follow the “Safety Precautions” written at the beginning of the instruction manual.

•If the equipment is used in a manner not specified by the manufacturer, or if the instructions are not

followed, the protection provided by the equipment may be impaired, causing malfunction, personal injury,

or property damage.

•Do not discard the instruction manuals and keep them handy for easy reference.

Label Attached to the Product

The label attached to the products shown below indicates that attention should be paid to use of these products. Before

using a product with the label attached, be sure to carefully read the manual.

Photobiological safety

label

IEC62471 RISK GROUP2

Possibly hazardous optical radiation

emitted from this product.

Do not stare at operating lamp.

May be harmful to the eyes.

CAUTION

English label

Groupe de risque 2 ATTENTION: possibles émissions de

rayonnements optiques de cet appareil. Ne regardez

pas la source de lumière, peut endommager vos yeux.

French label

Caution: Do not look into the LED light.

Possibly hazardous optical radiation emitted from this product.

Do not stare at operating lamp.

May be harmful to the eyes.

RISK GROUP 2 IEC62471: 2006

Label Position

P2-CIA Coaxial Epi Illuminator P2-FIR Ring Fiber Illumination Unit

C-FDF Flexible Double Arm Fiber

Illumination Unit

C-FID2 Double Arm Fiber Illuminator

French label

English label

French label

English label

French label (opposite side)

English label

French label

Safety Precautions

Heat caution label

Caution: Heat of the empty cube

Immediately after diascopic observation using an epi-fluorescence attachment, the front face

of the empty cube may become hot, resulting in burns. Do not touch the front face of the

empty cube. Replace the empty cube several minutes after finishing the diascopic

observation.

Label Position

Empty cube of the epi fluorescence attachment

Light leakage

caution label

Caution: Light leakage

Do not use the epi-fluorescence attachment when the filter cube replacement cover is open.

Harmful light including ultraviolet light may leak and affect fluorescence observation.

Label Position

PHOT

P2-EFLM Motorized Epi Fluorescence P2-EFLI Epi Fluorescence

Attachment Attachment

Fall caution label

Caution: P2-FMDN Focus Mount attachment orientation

Attach the P2-FMDN Focus Mount to the stand so that the focus mount faces the front of the

stand as shown in the figure.

If the focus mount is attached in the incorrect orientation, the stand may fall over. This may

result in damage to the device or unexpected injury.

Label Position

P-PS32 Plain Stand, P-DSL32 LED Diascopic Illumination Stand,

P-DSF32 Fiber Diascopic Illumination Stand

Heat caution label

Light leakage

caution label

Light leakage

caution label

Fall caution label

Safety Precautions

vii

Biohazard label

Caution: Biohazard

This symbol calls your attention to the following:

•The product may become biohazardous if a sample contacts the product.

•To avoid exposure to biohazard, do not touch contaminated parts with your bare hands.

•Decontaminate the contaminated parts according to the standard procedures for your

facility.

Label Position

P2-PB Plain Base, P2-DBL LED Diascopic Illumination Base,

P2-DBF Fiber Diascopic Illumination Base

P-PS32 Plain Stand, P-DSL32 LED Diascopic Illumination Stand,

P-DSF32 Fiber Diascopic Illumination Stand

WARNING and CAUTION Symbols

Safety instructions in this manual are marked with the following symbols to indicate their importance. For your safety, always

follow the instructions marked with these symbols.

Symbol Description

WARNING Disregarding instructions marked with this symbol may lead to serious injury or death.

CAUTION Disregarding instructions marked with this symbol may lead to injury or property damage.

Other Symbols

Indicates information you should note or comply with to prevent defects or malfunction of this product.

Indicates information you should be aware of in using this product, as well as other useful information.

Biohazard label

Safety Precautions

viii

WARNING

1 Do not disassemble.

Disassembling this product may result in electric

shock or malfunction. Malfunction and damage due

to disassembling or modification are unwarranted.

Do not disassemble parts other than those

described in this manual. If you experience

problems with this product, contact your nearest

Nikon representative.

2 Read the instruction manuals carefully.

To ensure safety, carefully read this manual and the

manuals for other equipment to be used with this

product. Particularly, all warnings and cautions

given at the beginning of each manual must be

observed.

Safety is a top design priority for Nikon products.

Safety is ensured as long as the user observes all

of the warnings and cautions given in the manuals,

and uses the system only for its intended purpose.

However, failure to heed the warnings and cautions

given in the manuals, subjecting the system to

shock or impact, or attempting to disassemble the

system may result in unexpected accidents and

injury.

Product with an epi-fluorescence attachment:

The light source used for epi-fluorescence microscop

(HG Precentered Fiber Illuminator) requires special

care during handling because of its characteristics.

Be sure to refer to the manual for the light source

being used.

3 Check the input rating.

For devices that use a power supply, check that the

rating of the power supplied to the device matches

the power used by the device. If the input rating

does not match, do not use the power supply and

contact your local representative. Using an

incorrect power supply may damage the device.

4 Notes on the power cord and AC adapter

Be sure to use the specified power cord and AC

adapter. If not, it may result in malfunction or fire.

This product is classified as having Class I

protection against electric shock. Make sure this

product is connected to an appropriate protective

earth terminal.

See Chapter 5, “1 Performance Properties” for the

specified power cords and AC adapters.

●To prevent electric shock, always turn off the

power switch (Press to the “O” position.) for the

device before connecting or disconnecting the

power cord.

5 Heat from the illuminator

During and immediately after a period of

illumination, the lamp and the periphery of the lamp

(including the lamphouse) are very hot if the light

source of the illumination device is a mercury lamp.

●Do not touch the lamp or the periphery of the

lamp during or immediately after a period of

illumination. There is a risk of burn if you touch

the hot area.

●Always attach the lamphouse cover when using

this product.

●Make sure the lamp and the periphery of the

lamp have cooled sufficiently (which may take

approximately 30 minutes) before replacing the

lamp.

●Do not place fabric, paper, or highly flammable

volatile materials (i.e. gasoline, petroleum

benzine, paint thinner, and alcohol) near the

lamphouse while the lamp is on or for about

thirty minutes after it was turned off. Because

the lamp is very hot, ignition or fire may be

induced.

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