Nikon LABOPHC-POL Bedienungsanleitung

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CAUTIONS

oAvoid sharp knocks!

Str

-free glasses

Handle

croscope gently,

taking

care

to avoid sharp knocks.

f)Wh

en carryi

the

microscope

When carry ing

micro

scop

hold its

arm

with

one

uppo

rting

the microscope bJse with the u

er.

The

ument weighs

about

8 kg,

8 Place for using

Avoid

the use

the m

icr

uscope in a dusty

ace, where it

subject

brat

ons

exposed to high t

emper

atur

es,

rY1

lstu

dire

ct s

light.

o Power source voltage

-For

European

trict

onl

Make s

ure

the

wer sour

voltage,

nov

240V

, bY mea

the i

nput

olt

age

change-over

switch

wh ich is

the

bottom

the microscope b

o Exchanging

the

lamp bulb and fuse

Befor

e replacing

,he

lamp

bulb

(6V-20WI

se,

turn

F the power

S'VIIit

ch and

disconnect

the pl

the

power

source

cord.

such cases as

replacement, do not

touch

the lamp

bulb

ith

bare hands, im-

mediately

after

putti

ng o

the lamp.

oDirt on

the

lens

Do n

ave

dust ,

dirt

finger mar

e lens surfaces.

They

will

prevent

from

clcar obser

va-

tion

of the

spe

cimen im

age

The o

pti

eleme

thi

s microscope

being cons

tru

cted of stra

-free

glass

particula

caution

handling

the

objectives and condenser len

ses

not

cause strain to them.

«;)

Focus

knobs

Never

attempt

adjust

e l ightn

ess

the ri

ght

- and

focus

obs by

rning the one,

while

holding

the other in

this mod

scope, bec

using

disorder.

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CARE AND MAINTENANCE

oCleaning

the

lenses

clean the lens surfaces, remove dust

using a soft brush or gauze.

Only

for

removing finger marks

grease, should

soft

cotton

cloth,

tissue or gauze

lightly

moistened

with

absolute alcohol

(metha

ethanol) be u

For

cleaning the objectives and immersion

oil

use

only

xylene.

Obser

icie

caution in handling

alcohol and

xylene

Cleaning

the

painted surfaces

Avoid

the

use

any organic solvent

(for

example, thinner, ether, al

cohol,

xylene

etc.)

r cleaning the painted surfaces and

plastic

parts

the

instrument.

@)Never

attempt

dismantle!

Never

attempt

dismantle

the

instrument

to avoid the

possibility

impairing

the

era

tional

efficiency

and accuracy.

O 'When

not

use

When not in

use

ver the instr

ument

with

the accessory

viny

l cover, and store

in a

placeJree

from

moisture and fungus.,

especially recommended

that

the

objectives and eyepi

eces

kept

airtight

container cont

desiccant.

oPeriodical checking'

To maintain the performance

the instru-

ment,

we recommend to check the ins

tru

ments periodically.

(For

details of

thi

check, contact

our

ency

CONTENTS

NOMENCLATURE

II.

ASSEMBLY

III.

PREPARATION

Interpupillary

Distance

Adju

tment.

Diopter

Adjustment.

3. Optical Path Change-over in the

Trinocular

Eyepiece

Tube

"TP"

4. Centering the Objectives

5. Centering the Condenser Lens.

Orientation

the Dia-polarizer

IV.

MICROSCOPY

1. Operating Procedure .

Manipulation

Each E

ment .

Focusi

ndenser aperture diaphragm

3) Field diaphragm.

4) Circular graduated stage

Objectives

Eyepieces

hro

mat strain-free condenser

8) Be

rtrand

lens

9) 1

tint

plate

10) Dia-polarizer and analyzer

...

11)

Filters.

Illumination

system

PHOTOMICROGRAPHY.

VI.

ACCESSORIES.

5enarmont

Compensator .

Quartz

Wedge

Monocular

Eyepiece

"AP"

4. Universal

epi-illuminat

or .

Attachable

Mechanic

Stage

Type

"E".

4f)

VII.

TROUBLE

SHOOTING

TABLE

REFERENCE

ELECTRIC

SPECIFICATIONS

(j)

\I)

4l;)

4ll

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NOMENCLATURE

Binocular eyepiece tube

"BP"

Interpupillary distance

scale

Eyepiece

tube

clamp

screw

---,

Diopter

ring

Centering nosepiece

---

Achro~m;a~t~P

o~~~~

(strain~free

)

Circular stage

Circular

graduated

stage

clamp screw

Vernier

Achromat

strain-

con

denser

Bertrand

lens I

Analyzer

out

knob

Intermediat

tube

clamp screw

Coarse k

nob

Fine focus

knob

Orient

tion

plate

Filter

rec

eptacle

Fig. 1

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Analvzer

rota

ring

ediate t

ube

"P"

Analyzer

clamp

screw

mpensator

slot

1/4 " &

tint

Condenser

focus

knob

Nosepi

ece

cen

tering

screw

clip

Torque

adjustment

ring

Lamp

cke

Field

diaphragm

control

ring

V-POL stand

rotation

clamp

screw

Condenser

centering

screw

Condenser

aperture

control

ring

Condenser

clam

p sere

.,."

Field lens

Brightness

control

dial

(including

power

switch)

Fig. 2

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II.

ASSEMBLY

• To assemble

the

microscope, follow

the

procedure

the

order

given:

Bottom

the

Base

eyepiece

~'=-n-se

~'~t

~t:':h-e-e':'y-

ie-c-e-C=-=

FWCC-

""-=O

-C::-M"""--

.....

into

right-hand slee

ve.

fitting

.......

sta

the

eyepiece

the

right-h

and

notch of

the

sleeve.

Int

the

left

-hand

sleeve, insert

IheCFW

1111

I •

.-.

~/4

~&~t_in.:.t.:.p

.:.

l~at

____

Remove

the

screw

the

side of

the

1/4

e of

the

1/4

A &

tint

plate

and

insert it

into

pensat

slot

the

ermediate

tube

"P",

facing

the

posit

ion

ing

Input

voltage c

han

ge-over gro

e to

ward

the

operator

side.

switch

Reatwch

the

emov

ed scr

ew.

(For

European districts only)

sure

power

source

~ ~

t~p

__________

A.:.c

h~r~o.:.m

~b~je

=-C~t

~iv~e

__________

vol(age,

220

0V.

means

----

the

input

wge

change-ov

Mount

the

objectives

the

nose-

itch

the

botto

the

piece in such posi

tions

that

eir

ro'icroscopp. bnse.

mvgnifying

powe

r increases

the

nosepiece

revo

lved

clockwise.

Bottom

the

Bas8 I

-=S

:.cp

-=-:::

:::

_ _

m:::e

li::.p

Place

the

clip

the

stage using

the

swg

c surfClce. !

Stage clamp scl'ew

---

--I1JIl

1i~;i~i;~

~~~

,~m~~~t~;;e~,~t~t~h;e~St

~g~e

~o~n

-1

the

circu

dovetail of t

sub-

stClg

Clamp

the

rew.

microscope.

mnn

ulJte

,,,

,rl;

"t-

jng screw

on~

foot

the

botto

m o f

the

mic

roscope

base.

chromat

condense,

nsen 10

con

denscr facing

Ihe

ape

rtu

num

ber

plate

towar

d the

operator.

Fasten the

clamp

scr

the l

eft

side

carrier.

Aperture

mber

pl;''',_ _

____

Dia

-p

,-,

a::.

-,--

taJ

----

-=-

,---o

iz--,-e

;..

--.,.------

-Gm

fl'2'3

After

centering

jec-

ves

condenser,

insert

the

dia-polar

i7..

inlO t

bottom

the

condenser.

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(J)

Eyepiece

tube

Attach

the

eyepiece

[Ube

intermediate

lUbe "

fitting

the

notch

the

circular

dovetai

l on

the

end

the

clamp

screw

sten

the

amp

screw.

Eyepiece

tube

clamp

screw

®Intermediate

tube

Atlach

the

int

mediat

tube

"P"

arm

of the

V-POL

stand,

lling

the

notch

e c

lar

ove

tail

the e

[he

clamp screw. Fasten the

clamp

screw.

ntermediate

tube

clamp screw

Power source

Halogen lamp

bulb

(6V-20W)

the

lamp

bulb

with

its

pins

int

the

accePting

holes in

the

socket.

te:

Don't

touch

the

sur-

direct

with

the

fin-

ger.

®Lamp

socket

-Insert

socket

into

the

recep-

tacle on

the

rear

the

baSE!.

-.:...:

lte

_ _

':O::

Place

the

filler

the field

ens.

Fig. 3

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III.

PREPARATION

1. Interpupillary Distance

ment

Place a spec

the stage, and f

us on the

specimen,

As shown in Fig. 4, adjust the interpupillary

distance, so that both the right and left v

fieldsbecome on

Fig. 4

2. Diopter Ad

just

ment

Rotate the diopter ring on the eyepiece CFW

l OX e M until the cro

lines are seen clea

(Fig 5)

Diopter

rin

--.

Fig. 5

(For

binocular ob

rvation)

1) Mo

unt

the

spe

cimen on the st

age

. Swing

the objec

ve l OX

int

o p

iti

on,

d bring

the

imen

age

int

o f

us l

king

into

the right-hand eyepiece.

Without

manipulating

the

arse-and-fine

focus

ob,

turn

the

er rtng on the

left

-ha

eye

focus on the speci·

men.

3. Optical Path Change-over

the

Trinocular Eyepiece Tube

''TP''

(Fig. 6)

ical

photo

ervation

Observati

~Jl

100

% tube :

OPti

<lIh

hang

e-o

ver

knob

Fig

Since Ihe CF eyepieces

high eye·

int

type. it

not n

55ar

y for the

use

putti

on h

specta

es to

move

em.

Only fold do

the eyeguard rubber.

(Fig 8)

Fig. 7 Fig. 8

4. Ce

nte

ll9

e Objectives

1) Place the specimen on the st

age

d rocus

on the specirnen. Bring

all

pro

pri

ate tar-

get 10 the ce

er of the

cro

lin

in the

eyepiec

Insert the cenlering

too

ls in the cente

rewson the n

l;!p

e. (Fig. 9)

n te

rin

screw

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Rotate

the

stage abo

180

0, and the target

displaced fr

the center

the cross

line

Move

the

obj

ective using the cente

tools

that

the center of the cross

lin

com

to one half pos

ition

the

displace

ment

the target. IF 1

10)

Rotate

180·

.-+--

-t

hal'

Target--.

------

posItIon

the

displacemen

Fig.

• Repeat the

ove procedure

two

three

times, and the rotati

nter

the s

coinci

des

with

thl;! cross lines center.

• Carry

out

ntering

for

eac:h

objective.

15.

ntaring

the

Condenser Lens

1) Close the field diaphragm

the

micro

scope

bas

its smallest site

mea

the field diaphragm

control

ring. Rotate

the condenser focus knob to move the

cond

ense

r vertically so t

t a sharp i

mage

the field diaphragm is for

rned

on t

specimen surface.

2) Bring the field d

phragm image to the

center of the field

view

mea

the condenser cen teri

screws.

(Fig

-l

fl )

Ctlange over to the objective

40x,

cmd

adjust the field di"phragm

that the

age

the

aphragm is about the

same

the

eyepie

viewf

ield stop,

shown in

Fig.

centered,

use

the con-

denser centering

rews

again.

Image

field

diaphragm

[

LEyepiece

----

I:l

Eyepiece

viewfield

StOP

viewfield

stOP

Fig. 11

Orientation

the Dia-polarizer

Plac t

specimen on the

stage

, focus on

the specim

using objective 40 X.

Set the

ana

lyzer

sca

le on the

int

ermediate

tub

e " P" to O.

Insert the dia-po

rizer

int

o the

bott

the conden

shown in Fig. 12.

Oia-polarizer-

Fig.

4) Remove the eyepiece from the sleeve.

Observing the e

xit

pupil

of obj

tive

ins

ide,

rotate

the dia-polarizm

form

a dark cross image on the exit

pupil

shown in Fig. 13.

Dark

cross image

Fig.13

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IV.

MICROSCOPY

r1. Operating Procedure •

Turn

the

brightness

control

dial

(including

power

switch) to

light

the

lamp.

Bring

the

analyzer and

the

Bertrand lens

out

the

optical

path. (Refer

P. 13 & 14)

3) Place

the

specimen on the

stage

and swing

the

10X

objective

into

position. Focus on specimen.

Adjust

the

interpupillary

distance and

diopter.

(Refer

Place

the

filter

the

field lens.

6) Carry

out

the centering procedure

for

the

objective. (Refer

Carry

out

the centering procedure

for

the con-

denser. (Refer

Bring

the

analyzer

into

the

optical

path.

9) Swing in

the

objective

used

and refocus on

specimen.

1'0) Brightness

voltage. IS adjusted

changing the lamp

Table 1

Ortho

sco

pic

microscopy Conoscopic

microscopy

Top lens

higher

ndenser

4 X

lower

OUT

Bert

and

OUT

Aperture

diaphragm

lOX

higher

80%

the

numerical aperture

the

objective

Circumscribed

the circumfer·

enee

the

conoscopic

field

view

(or fully

opened)

4x or

lower

Fully

opened

Field

diaphragm

lO X

high

Circumscribed

the

circumference

the eyepiece field

of view

Circumscribed

the circumfer·

enee of

orthoscopic

field of

ew4 x or

lower Fully

opened

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