Nikon Eclipse e100 Bedienungsanleitung

Microscope

ECLIPSE E100

Instructions

M413 E 06.10.NF.1

Thank you for purchasing this Nikon product. This instruction manual is for the users of the

Nikon Microscope ECLIPSE E100 describing basic operation of the microscope. To ensure

correct usage, please read this manual thoroughly before using the microscope.

• It is prohibited to reproduce or transmit this manual in any form without the prior

consent of Nikon.

• The instructions and specifications in this manual are subject to change without

notice.

• Although every effort has been made to ensure the accuracy of this manual, if

you find that any part of this manual is unclear or incorrect, contact your nearest

Nikon representative.

• Some ECLIPSE E100 microscope sets may come with different components

and accessories from those shown in this manual.

• Also read the manuals for the products used with the microscope, for example,

the Nikon photomicrographic equipment.

CAUTION

WARNING

Warning and Caution Symbols Used in This Manual

Symbols Labeled on the Product

Though Nikon products are designed to provide you with utmost safety during use, incorrect usage or

disregard of the instructions may cause personal injury or property damage. For your own safety, read the

instruction manual carefully and thoroughly before using the product. Do not discard this manual. Always

keep it near the product for easy reference.

Inside this instruction manual, safety instructions are indicated with the symbols shown below. Be sure to

follow the instructions marked with these symbols for your safety.

Symbol Meaning

Disregarding instructions marked with this symbol may lead to death or

serious injury.

Disregarding instructions marked with this symbol may lead to injury or

property damage.

Symbol Meaning

Caution! Hot surface

This symbol label attached near the field lens unit (the lamp is set

underneath the field lens unit) and on the bottom surface of the base plate

(the lamp is set above this place) reminds you of the following.

WARNING: Risk of burn injury. The lamp and its surroundings (including

the field lens unit) become hot during use. Do not touch them

while the lamp is on and for thirty minutes after the lamp is

turned off.

CAUTION: The lamp and its surroundings remain hot even after the lamp

is turned off. Make sure that they are sufficiently cool before

replacing the lamp.

Caution!

This symbol label attached near the AC inlet reminds you of the following.

CAUTION: Make sure that the input voltage set by the voltage selector

matches the voltage provided in your region.

The voltage selector on the microscope sent to the United

States is set to “120V” at the factory.

WARNING

Common Sense Safety Instructions

1. Purpose

Use this microscope only for microscopic observation.

Do not use this microscope for any other purpose because the protection provided

by the equipment may be impaired.

2. Never Disassemble the Microscope

There are no user serviceable parts inside the microscope. Disassembly will void the warranty, and

could degrade the performance, cause electrical shock or personal injury, or damage the instrument.

If you have a service problem, contact your nearest Nikon representative.

4. Use the Specified Lamp, Fuse, and Power Cord

Use the specified lamp and fuse. For the microscope with the nameplate showing [100/110/120 V], use

the power cord provided. For the microscope with the nameplate showing [220/230/240 V], use the

specified power cord. Using an incorrect lamp, fuse, or power cord may damage the instrument or cause

a fire. (Also see p. 41-42 on power cord.)

●Specified Lamp

Halogen lamp 6V-20W (PHILIPS 7388 or OSRAM HLX64250)

●Specified Fuse

• For the microscope with the nameplate showing [100/110/120 V 〜

〜]:

250 V, 1 A, time-lag low-breaking type, 5X20 miniature fuses (two pieces)

• For the microscope with the nameplate showing [220/230/240 V 〜

〜]:

250 V, 0.5 A, time-lag low-breaking type, 5X20 miniature fuses (two pieces)

3. Check the Input Voltage

The input voltage is indicated in two places at the rear of the microscope: on the nameplate and above

the AC-IN socket. Confirm that these input voltage indications correspond to the voltage provided in your

region. If not, follow one of the instructions below. The use of microscopes with the different input

voltage indications will cause overcurrent and overheating, which may result in fire or severe damage to

the microscope.

●If the voltage indication on the nameplate differs:

Do not plug in the microscope. Contact your nearest Nikon

representative.

●If the voltage indication above the AC-IN socket differs:

Refer to p. 26 and change the input voltage setting before

turning on the power switch.

• For the microscope with the nameplate showing

[100/110/120 V 〜

〜]:

The voltage can be set to: AC 100 V, 110 V or 120 V.

• For the microscope with the nameplate showing

[220/230/240 V 〜

〜]:

The voltage can be set to: AC 220 V, 230 V or 240 V.

Input Voltage

Indications

5. Turn Off Power Switch before Assembling the

Microscope, Replacing the Lamp or Fuse, and

Plugging in or Unplugging the Power Cord

CAUTION

WARNING

CAUTION

Turn off the power switch before you plug or unplug the power cord to prevent electrical shock or fire.

Also turn off the power switch and then unplug the power cord before assembling the microscope, and

before changing the lamp or fuse. To turn off the power, turn the power switch to ○.

6. Keep the Microscope Free of Moisture and Foreign Matter

Keep the microscope free of moisture to prevent short circuiting that could result in overheating or other

malfunctions. If water splashes on the microscope, immediately turn off the power switch (turn the switch

to ○) and unplug the power cord. Then, wipe off the water with a dry cloth. Short circuiting can also

result when foreign matter is trapped inside the microscope. If foreign matter or water has entered the

microscope, do not use the microscope and contact your nearest Nikon representative.

7. Heat at the Light Source

The lamp becomes hot during use. Do not remove the field lens unit while the lamp is on, and be sure

the lamp has been off for 30 minutes before touching it.

• When changing the lamp bulb, make sure that the lamp is cool enough to touch (the light should be

off at least 30 minutes).

• Do not touch the lamp while it is on or until the lamp has been off for 30 minutes, as doing so could

result in burns.

• Never bring cloth, paper or flammable volatile substances such as gasoline, petroleum benzine,

acetone, thinner, or alcohol near a hot lamp, as a fire could result.

8. Stage Rack

The rack for the stage protrudes during use. Be careful not to hit the rack with your hand or other parts of

your body when handling the microscope as you may get hurt by an edge of the rack.

Rack

9. Installation

10. Carrying the Microscope

This microscope is a precision instrument. Using the microscope in an unfavorable environment could

result in malfunctions or degraded performance. Consider the following conditions when choosing the

installation location.

• Observation conditions are better if light from windows and bright room light can be avoided.

• Install the microscope in a location with a room temperature of 0˚ to 40˚ C and with a maximum

relative humidity of 85%. High temperature and humidity are to be avoided because they promote

mold growth and condensation, which may damage the microscope.

• Dirt and dust degrade optical performance and are to be avoided.

• Vibrations in the environment will degrade the image. Install the microscope in a location free of

vibrations.

• Install the microscope on a solid table and keep the microscope level.

• This microscope emits a feeble electromagnetic wave. Do not place a precision electronic device

near the microscope as precision could be degraded. Also, avoid placing a radio or TV near the

microscope as reception of sound and images may be hampered.

• Install the microscope in the location of 10 cm or more away from the surrounding walls.

• Install the microscope in the place that allows easy detachment of the power cord from the AC inlet

in the event of emergency.

This microscope is a precision instrument. Handle it gently.

Strong shocks and forcible operation will damage the

instrument. Shocks to the objectives, especially, could degrade

image precision.

• When carrying the microscope, hold it at its arm with both

hands.

• Do not hold the focus knobs, the eyepiece tube, or the

stage. These parts could easily come off and could result

in malfunctions.

11. Handling the Lamp

Do not touch the glass part of the lamp with bare hands. Wear gloves or use a cloth when handling the

lamp so as not to leave fingerprints on the surface. Wipe off any fingerprints or stains using a clean

cloth moistened with alcohol. Fingerprints will etch into the hot surface of the lamp and reduce the

brightness, damage the lamp or reduce its service life.

Handle the lamp gently. Shocks and vibrations will damage the lamp or reduce its service life.

When changing the lamp, be sure that the contact is not damaged. If the contact is damaged, the lamp

may not light up or may overheat. Insert the lamp’s contact pins fully into the socket holes. If the pins

are loose, the lamp could come off or result in a contact failure, which will cause overheating or smoke.

Also, make sure that the field lens unit is securely attached.

12. Focus Knobs

Do not turn the right and left focus knobs simultaneously in opposite directions. Do not turn the coarse

focus knob any further after the stage has been moved up or down to its limit. These operations will

damage the focusing mechanism.

14. Shipping Clamps

The microscope is held tightly by the clamps during shipment.

Be sure to remove the clamps before use. For details, see p. 27.

Do not turn the knobs in opposite directions.

WARNING

13. Oil-Immersion Observation

Use only a minimum quantity of oil. If too much oil is applied, surplus oil could flow out to the stage and

the condenser which could lead to degraded performance.

When using petroleum benzine or absolute alcohol to wipe off immersion oil or

to clean the lenses, follow the instructions provided by their manufacturers.

Absolute alcohol and petroleum benzine are inflammable. Take great care

when handling them.

Shipping Clamps

Contents

Warning and Caution Symbols Used in This Manual ････････････････････････････2

Symbols Labeled on the Product ･････････････････････････････････････････････2

Common Sense Safety Instructions･･･････････････････････････････････････････3

I. Nomenclature of Each Part ･･･････････････････････････････････････････8

II. Switches and Controls･･･････････････････････････････････････････････10

III. A Swift Microscopic Procedure ･････････････････････････････････････12

IV. Microscopy (Detailed Procedure) ･･･････････････････････････････････14

V. Miscellaneous Operations ･･･････････････････････････････････････････22

1. Oil-Immersion Observation ･･･････････････････････････････････････････････22

2. Adjusting the Torque of the Coarse Focus Knob ････････････････････････････24

3. Photomicrography and TV Microscopy･････････････････････････････････････24

4. Cord Hangers ･･････････････････････････････････････････････････････････24

VI. Assembly ･････････････････････････････････････････････････････････････25

1. Assembly of Standard Set････････････････････････････････････････････････26

2. Assembly of Additional Components･･･････････････････････････････････････28

1) Condenser･･･････････････････････････････････････････････････････････28

2) Objectives ･･･････････････････････････････････････････････････････････29

3) Specimen Holder ･････････････････････････････････････････････････････29

4) Eyepiece and Eyepiece Pointer･････････････････････････････････････････29

5) Monocular eyepiece tube ･･････････････････････････････････････････････29

6) Other Accessories ････････････････････････････････････････････････････29

3. Replacement of Consumable Materials ････････････････････････････････････30

1) Replacing the Lamp･･･････････････････････････････････････････････････30

2) Replacing the Fuse ･･･････････････････････････････････････････････････31

VII. Optical Characteristics ･･････････････････････････････････････････････33

VIII.Troubleshooting tables ･･････････････････････････････････････････････35

IX. Care and Maintenance ･･･････････････････････････････････････････････40

X. Specifications ････････････････････････････････････････････････････････41

The microscope is made up of the following components.

Basic unit

Eyepieces

Screwed on to the eyepiece tube.

Eyepiece tube

This is a binocular eyepiece tube. A trinocular eyepiece tube is available for

photomicrography and TV microscopy. Amonocular eyepiece tube can be used also.

Objectives

Objectives with various magnifying powers are available.

Condenser

Used for condensing light. The condenser should be positioned slightly lower

than its upper limit.

Adjust the aperture diaphragm lever according to the objective.

Field lens unit

Pull the field lens unit upward when changing lamp.

Lamp

Halogen lamp of 6 V-20 W is used.

Fuse

For the microscope with the nameplate showing [100/110/120 V], 250 V, 1 A, time-lag low-

breaking type, 5X20 miniature fuses (two pieces) are used.

For the microscope with the nameplate showing [220/230/240 V], 250 V, 0.5 A, time-lag low-

breaking type, 5X20 miniature fuses (two pieces) are used.

Power cord

Use the power cord provided.

Nomenclature of Each Part

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