Sartorius Vivaspin Turbo 15 RC Bedienungsanleitung

Instructions for Use

Vivaspin®Turbo 15 RC

Centrifugal Ultrafiltration Devices for Research Use

2563320-000-02

Contents

Instructions for Use Vivaspin®Turbo 15 RC 3

Contents

1 About these Instructions.................................................5

1.1 Scope .................................................................5

1.2 Target Groups.........................................................5

1.3 Symbols Used.........................................................6

1.3.1 Warnings in Operation Descriptions...........................6

1.3.2 Other Symbols.................................................6

2 Safety Instructions .......................................................7

2.1 General Functions ....................................................7

2.2 Personnel Qualification ...............................................7

2.3 Significance of these Instructions.....................................8

2.4 Functionality of the Product ..........................................8

3 Product Description .....................................................9

3.1 Product Overview.....................................................9

3.2 Product Symbols.....................................................10

4 Process Preparation......................................................11

4.1 Scope of Delivery .....................................................11

4.2 Unpacking ............................................................11

5 Operation...............................................................12

5.1 Pre-Rinsing the Product .............................................12

5.2 Sanitizing the Product ...............................................12

5.3 Performing Filtration.................................................13

5.3.1 Applying the sample..........................................13

5.3.2 Inserting the Product into the Centrifuge ....................14

5.3.3 Performing Filtration .........................................14

5.3.4 Removing the Sample ........................................15

5.4 Desalting or Buffer Exchange ........................................15

6 Storage .................................................................16

6.1 Storing the Product ..................................................16

7 Disposal.................................................................17

7.1 Decontaminating the Product .......................................17

7.2 Disposing of the Product.............................................17

Contents

4 Instructions for Use Vivaspin®Turbo 15 RC

8 Technical Specifications ................................................18

8.1 Dimensions ..........................................................18

8.2 Materials .............................................................18

8.3 Ambient Conditions..................................................19

8.4 Operating Conditions................................................19

8.5 Equipment Required................................................20

8.5.1 Pipettes......................................................20

8.5.2 Centrifuges ..................................................20

8.6 Sanitizing Methods .................................................20

8.7 Chemical Compatibility ..............................................21

8.8 Typical Performance Characteristics.................................22

8.8.1 Typical Concentration Conditions ............................22

8.8.2 Examples of Times to Concentrate 30x at 20 °C and Solute

Recoveries....................................................23

About these Instructions

Instructions for Use Vivaspin®Turbo 15 RC 5

1 About these Instructions

1.1 Scope

These instructions are part of the product. These instructions apply to the

following versions of the product:

Vivaspin®Turbo 15 RC Quantity Prod. no.

5 kDa 12 | 48 VS15TR11 | VS15TR12

10 kDa 12 | 48 VS15TR01 | VS15TR02

30 kDa 12 | 48 VS15TR21 | VS15TR22

50 kDa 12 | 48 VS15TR31 | VS15TR32

100 kDa 12 | 48 VS15TR41 | VS15TR42

1.2 Target Groups

The instructions are addressed to the following target groups. The target

groups must possess the knowledge listed below.

Target Group Knowledge and Qualifications

Operator The operator is familiarwith the device and the

associated work processes. The operator understands

the hazards which may arise when working with the

device, and knows how to prevent them.

About these Instructions

6 Instructions for Use Vivaspin®Turbo 15 RC

1.3 Symbols Used

1.3.1 Warnings in Operation Descriptions

NOTICE

Denotes a hazard that may result in property damage if it is not avoided.

1.3.2 Other Symbols

t  Required action: Describes actions that must be carried out.

The actions in the sequence must be carried out in succession.

y Result: Describes the result of the actions carried out.

Safety Instructions

Instructions for Use Vivaspin®Turbo 15 RC 7

2 Safety Instructions

2.1 General Functions

The product is intended for the ultrafiltration and | or diafiltration of biological

and aqueous solutions. The sample solutions and volumes used must be suit-

able for the product.

The filtration process must be carried out in a centrifuge. Macromolecules

that are sufficiently larger than the nominal pore size of the membrane are re-

tained above the membrane and progressively concentrated. The vertical

membrane inhibits membrane fouling while the built-in dead stop impedes

concentration to dryness and loss of sample.

The product is supplied non-sterile. It is intended for single use and must be

disposed of after one use.

The product is intended exclusively for use in accordance with these instruc-

tions. Any further use beyond this is considered improper.

Operating Conditions for the Product

The product may only be used for research purposes. Do not use the product

for in vitro diagnostic procedures or similar diagnostic procedures.

The product may only be used with the equipment and under the operating

conditions described in the Technical Data section of these instructions.

2.2 Personnel Qualification

Persons without sufficient knowledge in the safe use of the device can injure

themselves and others.

If a specific qualification is required for an activity:The target group is indicated.

If no qualification is specified: The activity can be carried out by the target

group “Operator”.

Safety Instructions

8 Instructions for Use Vivaspin®Turbo 15 RC

2.3 Significance of these Instructions

Failure to follow the instructions might have serious consequences, e.g. dan-

ger to individuals.

t Read the instructions carefully and completely. The instructions for action

build on each other.

t Ensure that the information contained in these instructions is available to

all individuals working with the product.

2.4 Functionality of the Product

A damaged product or worn parts can lead to malfunctions or cause hazards

which are difficult to identify.

t Only operate the product when it is safe and in perfect working order.

Product Description

Instructions for Use Vivaspin®Turbo 15 RC 9

3 Product Description

3.1 Product Overview

Fig.1: Product overview (example)

Pos. Description

1 Concentrator cap

2 Concentrator

3Twin vertical membranes

4 Filtrate container

5 Angular dead stop pocket

Product Description

10 Instructions for Use Vivaspin®Turbo 15 RC

3.2 Product Symbols

Symbol Description

Catalogue number

Batch code

Use by

Non-sterile product

Only suitable for research purposes

Do not re-use

Consult instructions for use

Temperature limits

Inhaltsverzeichnis

Andere Sartorius Laborausrüstung Handbücher

Sartorius

Sartorius Sartorius SWC Bedienungsanleitung

Sartorius

Sartorius Vivaspin Filtrate Bedienungsanleitung

Sartorius

Sartorius IS06BBE-SX Handbuch

Sartorius

Sartorius Maxicaps MR Virosart HF Bedienungsanleitung

Sartorius

Sartorius Vivaflow 50 Handbuch

Sartorius

Sartorius WZA623-NX Bedienungsanleitung

Sartorius

Sartorius CERTOMAT Tplus Bedienungsanleitung

Sartorius

Sartorius PICUS Bedienungsanleitung

Sartorius

Sartorius WZG Series Bedienungsanleitung

Sartorius

Sartorius Picus 2 Bedienungsanleitung

Sartorius

Sartorius PICUS Bedienungsanleitung

Sartorius

Sartorius Cubis MCA2.7S Bedienungsanleitung

Sartorius

Sartorius Andrew Alliance Stand+ Bedienungsanleitung

Sartorius

Sartorius MD8 16746-01--SHB Bedienungsanleitung

Sartorius

Sartorius Cubis MCA Series Bedienungsanleitung

Sartorius

Sartorius Cubis MSU116P Bedienungsanleitung

Sartorius

Sartorius Sartobind IEX MA 15 Bedienungsanleitung

Sartorius

Sartorius Midi Plus Bedienungsanleitung

Sartorius

Sartorius YDK04 Bedienungsanleitung

Sartorius

Sartorius 16274 Bedienungsanleitung

Sartorius

Sartorius Vivaspin 500 Bedienungsanleitung

Sartorius

Sartorius Proline Bedienungsanleitung

Sartorius

Sartorius Sterisart Universal Pump 16420 Bedienungsanleitung

Sartorius

Sartorius BioPAT Trace Bedienungsanleitung

Beliebte Laborausrüstung Handbücher anderer Marken

Qiagen

Qiagen DML 3000 Bedienungsanleitung

Queensgate

Queensgate NANOSCAN OP400 Programmierhandbuch

Conviron

Conviron GEN1000-GE Bedienungsanleitung

Parr Instrument

Parr Instrument 4560 Bedienungsanleitung

IKA

IKA IC control Bedienungsanleitung

Integra

Integra DOSE IT Bedienungsanleitung

Agilent Technologies

Agilent Technologies 5800 ICP-OES Bedienungsanleitung

Endress+Hauser

Endress+Hauser Cleanfit CPA875 Bedienungsanleitung

NI PXI-5422 Handbuch

Collomix

Collomix Aqix Bedienungsanleitung

SPEX SamplePrep

SPEX SamplePrep 6875 Freezer/Mill Series Bedienungsanleitung

Ocean Insight

Ocean Insight FLAME-NIR+ Bedienungsanleitung

Parker

Parker ALIGN-MG-NA Bedienungsanleitung

BD 644787 Bedienungsanleitung

DENTAURUM

DENTAURUM Compact Megaplus Bedienungsanleitung

Biuged Laboratory Instruments

Biuged Laboratory Instruments BGD 626 Bedienungsanleitung

VWR

VWR SAS Super IAQ Bedienungsanleitung

illumina

illumina MiSeqDx Bedienungsanleitung