Sartorius Vivaspin 500 Bedienungsanleitung

Instructions for Use

Vivaspin®500 and 2

Centrifugal Ultrafiltration Units for General Laboratory Use

3104647-001-00

Contents

Instructions for use Vivaspin®500 | 2 3

Contents

1 About these Instructions.................................................5

1.1 Scope .................................................................5

1.2 Target Groups.........................................................7

1.3 Symbols Used.........................................................7

1.3.1 Warnings in Operation Descriptions...........................7

1.3.2 Other Symbols.................................................7

2 Safety Instructions .......................................................8

2.1 General Functions ....................................................8

2.2 Personnel Qualification ...............................................9

2.3 Significance of these Instructions.....................................9

2.4 Functionality of the Product ..........................................9

3 Product Description ....................................................10

3.1 Vivaspin®500........................................................10

3.2 Vivaspin®2 ............................................................11

3.3 Product Symbols .....................................................12

4 Process Preparation.....................................................13

4.1 Scope of Delivery ....................................................13

4.2 Unpacking ...........................................................13

5 Operation...............................................................14

5.1 Pre-Rinsing the Product .............................................14

5.2 Sanitizing the Product ...............................................14

5.3 Applying the Sample.................................................15

5.4 Inserting the Product into the Centrifuge............................16

5.5 Performing Filtration.................................................16

5.6 Removing the Sample................................................16

5.7 Vivaspin®2 ...........................................................17

5.7.1 Removing the Concentrator from the Filtrate Container .....17

5.7.2 Reverse spin with Vivaspin®2 .................................18

5.8 Desalting or Buffer Exchange ........................................18

6 Storage .................................................................19

6.1 Storing the Product ..................................................19

Contents

4 Instructions for use Vivaspin®500 | 2

7 Disposal................................................................20

7.1 Decontaminating the Product ......................................20

7.2 Disposing of the Product............................................20

8 Technical Specifications ................................................21

8.1 Dimensions ..........................................................21

8.2 Materials .............................................................21

8.3 Ambient Conditions..................................................22

8.4 Operating conditions ................................................22

8.4.1 Filtration Volumes ............................................22

8.4.2 Centrifugation Limit Values...................................23

8.5 Equipment Required.................................................23

8.5.1 Pipettes.......................................................23

8.5.2 Centrifuges ...................................................24

8.6 Sanitizing Methods ..................................................24

9 Typical Performance Characteristics....................................25

9.1 Vivaspin®500....................................................... 25

9.2 Vivaspin®2 .......................................................... 26

10 Chemical Compatibility.................................................27

Instructions for use Vivaspin®500 | 2 5

About these Instructions

1 About these Instructions

1.1 Scope

These instructions are part of the product. These instructions apply to the

following versions of the product:

Vivaspin®500 PES Quantity Prod. no.

3 kDa 25 | 100 VS0191 | VS0192

5 kDa 25 | 100 VS0111 | VS0112

10 kDa 25 | 100 VS0101 | VS0102

30 kDa 25 | 100 VS0121 | VS0122

50 kDa 25 | 100 VS0131 | VS0132

100 kDa 25 | 100 VS0141 | VS0142

300 kDa 25 | 100 VS0151 | VS0152

1,000 kDa 25 | 100 VS0161 | VS0162

0.2 μm 25 | 100 VS0171 | VS0172

6 Instructions for use Vivaspin®500 | 2

About these Instructions

Vivaspin®2 PES Quantity Prod. no.

3 kDa 25 | 100 VS0291 | VS0292

5 kDa 25 | 100 VS0211 | VS0212

10 kDa 25 | 100 VS0201 | VS0202

30 kDa 25 | 100 VS0221 | VS0222

50 kDa 25 | 100 VS0231 | VS0232

100 kDa 25 | 100 VS0241 | VS0242

300 kDa 25 | 100 VS0251 | VS0252

1,000 kDa 25 | 100 VS0261 | VS0262

0.2 μm 25 | 100 VS0271 | VS0272

Vivaspin®2 Cellulose

Triacetate Quantity Prod. no.

10 kDa 25 | 100 VS02V1 | VS02V2

20 kDa 25 | 100 VS02X1 | VS02X2

Vivaspin®2 Hydrosart®Quantity Prod. no.

2 kDa 25 | 100 VS02H91 | VS02H92

5 kDa 25 | 100 VS02H11 | VS02H12

10 kDa 25 | 100 VS02H01 | VS02H02

30 kDa 25 | 100 VS02H21 | VS02H22

Instructions for use Vivaspin®500 | 2 7

About these Instructions

1.2 Target Groups

The instructions are designed for the following target groups. The target

groups must possess the knowledge listed below.

Target Group Knowledge and Qualifications

Operator The operator of the product is responsible for compliance

with safety requirements and workplace safety regula-

tions. The operator must ensure that anyone working with

the product has access to the relevant information and is

trained to work with the product.

1.3 Symbols Used

1.3.1 Warnings in Operation Descriptions

NOTICE

Denotes a hazard that may result in property damage if it is not avoided.

1.3.2 Other Symbols

t Required action: Describes actions that must be carried out.

The actions in the sequence must be carried out in succession.

y Result: Describes the result of the actions carried out.

8 Instructions for use Vivaspin®500 | 2

Safety Instructions

2 Safety Instructions

2.1 General Functions

The product is intended for the ultrafiltration and | or diafiltration of biological

and aqueous solutions. The sample solutions and volumes used must be

suitable for the product.

The filtration process must be carried out in a centrifuge. Macromolecules

that are sufficiently larger than the nominal pore size of the membrane are

retained above the membrane and progressively concentrated. The vertical

membrane inhibits membrane fouling while the built-in dead stop impedes

concentration to dryness and loss of sample.

The product is supplied non-sterile. It is intended for single use and must be

disposed of after one use.

The product is intended exclusively for use in accordance with these instruc-

tions. Any further use beyond this is considered improper.

Operating Conditions for the Product

The product is intended for general laboratory use.

The product may only be used with the equipment and under the operating

conditions described in the Technical Data section of these instructions.

Instructions for use Vivaspin®500 | 2 9

Safety Instructions

2.2 Personnel Qualification

Persons without sufficient knowledge in the safe use of the device can injure

themselves and others.

If a specific qualification is required for an activity:The target group is indi-

cated. If no qualification is specified: The activity can be carried out by the

target group “Operator”.

2.3 Significance of these Instructions

Failure to follow the instructions might have serious consequences, e.g.

danger to individuals.

tRead the instructions carefully and completely. The instructions for action

build on each other.

tEnsure that the information contained in these instructions is available to

all individuals working with the product.

2.4 Functionality of the Product

A damaged product or worn parts can lead to malfunctions or cause hazards

which are difficult to identify.

tOnly operate the product when it is safe and in perfect working order.

10 Instructions for use Vivaspin®500 | 2

Product Description

3 Product Description

3.1 Vivaspin®500

Fig.1: Product overview (example)

Pos. Description

1 Concentrator

2 Membrane

3 Filtrate container

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