THORLABS RSBF2 Bedienungsanleitung

Raman Spectroscopy Bundle

RSB1(/M,) RSBR1(/M),

RSBC1(/M), RSBF2, RSBA1

Operation Manual

Version:

Date: 1.2

07-Mar-2023

Foreword Table of Contents

1 General Information 1

21.1 Ordering Codes and Accessories 41.2 Requirements

2 Getting Started 5

52.1 Parts List

3 Operating Elements 7

73.1 RSB1(/M) Base Unit 83.2 RSBR1(/M) Front End for Reflective Measurements 103.3 RSBC1(/M) Front End for Cuvette Measurements 113.4 RSBF2 Fiber Input Adapter 123.5 RSBA1 Mounting Brackets for Cage System

4 System Setup 13

134.1 Mounting RSB1(/M) 154.2 Mounting Front Ends and Adapters 154.2.1 Mounting RSBR1(/M) 204.2.2 Mounting RSBC1(/M) 224.2.3 Mounting RSBF2 234.2.4 Mounting Cage Systems 244.2.5 Mounting on the SM1.5 External Thread of RSB1(/M) 244.3 Laser and Laser Driver 254.3.1 Laser 254.3.1.1 Alternative Excitation Wavelength 254.3.2 Optical Fiber 264.3.3 Laser Driver 264.3.4 Mounting the Laser 274.4 Connecting the RSB1(/M) to the PC

5 Operating Instructions 28

285.1 Laser Driver Settings 295.2 Software and Installation 315.3 Raman Measurements with the OSA Raman Software 315.3.1 Software Startup Screen 325.3.2 Software Configuration Procedure 335.3.3 Settings 355.3.3.1 Info Panel 365.3.3.2 Main Panel 415.3.3.3 Dark Image 415.3.4 System Calibration

3.5 Export Data 505.4 Experimental Measurements 505.4.1 Polystyrene System Test 515.4.2 Sample Measurement 525.4.3 Compatible Cuvettes for RSBC1(/M) 
Operating Principle 53
1 Analysis of Raman Spectra 556.2 Acquisition of Raman Spectra 586.3 Coded-Aperture Raman Spectroscopy 606.4 Parameters Affecting Spectra 
Maintenance and Service 64
1 Troubleshooting 
Appendix 68
1 Technical Data 698.1.1 RSB1/M and RSB1 Technical Data 708.1.1.1 RSB1/M and RSB1 Dimensions 728.1.2 RSBR1/M and RSBR1 Technical Data 738.1.2.1 RSBR1/M and RSBR1 Dimensions 758.1.3 RSBC1/M and RSBC1 Technical Data 768.1.3.1 RSBC1/M and RSBC1 Dimensions 788.1.4 RSBF2 Technical Data 798.1.4.1 RSBF2 Dimensions 808.1.5 RSBA1 Technical Data 808.1.5.1 RSBA1 Dimensions 818.1.6 Thorlabs Raman Bundle Technical Data 818.1.7 Laser Requirements 828.1.8 Technical Data Explained 828.1.8.1 Signal-to-Noise Characterization 848.1.8.2 Spectral Accuracy and Spectral Resolution 868.1.8.3 Amplitude Correction 888.2 List of Acronyms 888.3 References 908.4 Application Note Polarization Dependent Raman Spectroscopy 928.4.1 Optimizing Raman Signal via Collimator Rotation 938.4.2 Including Polarization Optics in the Excitation Path 958.4.3 Including Polarization Optics in the Raman Scattering Path 968.4.4 References 978.5 OSA Raman Software: General Instructions 978.5.1 Interface Overview 988.5.2 Customizing the Display 988.5.2.1 Zooming and Scrolling the Horizontal Axis

998.5.2.2 Zooming and Scrolling the Vertical Axis 998.5.2.3 Setting the Unit of the Horizontal Axis 998.5.2.4 Setting the Unit of the Vertical Axis 1008.5.2.5 Displaying the Secondary Vertical Axis 1018.5.2.6 Hiding the Menus and Full Screen Mode 1028.5.2.7 Displaying a Split View 1028.5.2.8 Displaying a Legend in the Data Display Area 1038.5.2.9 Dark Room Mode 1048.5.3 Acquiring Data 1048.5.3.1 Finding the Spectrometer 1048.5.3.2 Selecting the Instrument to Use 1058.5.3.3 Acquiring a Single Spectrum 1068.5.3.4 Repeated Measurements 1068.5.3.5 Averaging Spectra 1068.5.4 Movable Markers 1078.5.4.1 Moving Markers 1078.5.4.2 Marker Values 1078.5.4.3 Locking the Markers 1088.5.5 Fixed Markers 1088.5.5.1 Adding and Removing Fixed Markers 1098.5.5.2 Automatically Mark Peaks 1098.5.5.3 Value of Markers 1108.5.6 Working with Data in Traces 1108.5.6.1 Active Trace 1108.5.6.2 Trace Update Options 1128.5.6.3 User-Defined Update Options 1138.5.6.4 Viewing Properties of a Trace 1148.5.6.5 Reordering Traces 1148.5.6.6 Math Operations 1198.5.6.7 Functional Traces (Traces Defined by a Function) 1198.5.7 Saving and Loading Data 1208.5.7.1 Saving Spectral Data 1218.5.7.2 Loading Spectral Data 1218.5.8 Analyzing data 1218.5.8.1 Floating Windows 1228.5.8.2 Displayed Range and Threshold 1238.5.8.3 Peak Track 1258.5.8.4 Valley Track 1278.5.8.5 Statistics Analysis 1288.5.8.6 Curve Fit 1318.5.8.7 Baseline Correction 1328.5.9 Keyboard Shortcuts 

1338.5.10 General OSA Raman Settings 1338.5.10.1 Basic Settings 1358.5.10.2 Saving and Loading Settings 1388.6 Safety 1398.7 Return of Devices 1398.8 Manufacturer Address 1398.9 Warranty 1398.10Copyright and Exclusion of Liability 1408.11Thorlabs Worldwide Contacts - WEEE Policy 

We aim to develop and produce the best solutions for your

applications in the field of optical measurement techniques. To help

us to live up to your expectations and constantly improve our

products, we need your ideas and suggestions. We and our

international partners are looking forward to hearing from you.

Thorlabs GmbH

Warning

Sections marked by this symbol explain dangers that might result in

personal injury or death. Always read the associated information

carefully before performing the indicated procedure.

Please read this advice carefully!

This manual also contains "NOTES" and "HINTS" written in this form.

Attention

Paragraphs preceded by this symbol explain hazards that could

damage the instrument and the connected equipment or may cause

loss of data.

Note

Raman Spectroscopy Bundle Chapter 1: General Information

MTN022733-D02

Page 1

1 General Information

This manual describes the use of the Thorlabs components for Raman Spectroscopy, a spec-

trograph, different front ends and adapters, suitable for varying applications.

Raman spectroscopy is a technique that detects and analyzes inelastic photon scattering,

known as Raman scattering. Because Raman scattering is material specific and provides a mo-

lecular fingerprint, Raman spectroscopy can be used to identify specific chemicals or mo-

lecules.

One challenge in Raman spectroscopy has long been the low signal-to-noise ratio (SNR) due to

the very weak Raman scattering signal. The Coded-Aperture (CODA) technology can overcome

the challenge of the low SNR as it uses a two dimensional matrix with a defined pattern of mul-

tiple slits instead of a single slit input aperture. This coded input aperture allows data acquisi-

tion from a larger sampling volume than a single slit input aperture. This in turn, enables higher

sampling rates and an increased SNR. Please find more general information on Raman spec-

troscopy and background on Coded-Aperture systems in the chapter Operating Principle .

RSB1(/M) Raman Spectrograph

The Thorlabs RSB1(/M) Raman Spectroscopy Base Unit features such a highly optimized spec-

trograph based on the CODA technology with a pseudo Hadamard mask of order 64 and an at-

tached CMOS camera (not removable). The geometric size of the Coded-Aperture's active area

is 3.159 mm x 2.304 mm. Due to the Coded-Aperture technology and the spatial averaging in

the analysis, the Thorlabs Raman spectrograph RSB1(/M) is superior in the analysis of complex

mixtures.

Beyond the Coded-Aperture technology, the RSB1(/M) Raman Spectroscopy Base Unit is de-

signed for 785 nm excitation and a detection range of 500 cm-1 to 1800 cm-1. The RSB1(/M)

Base Unit can be purchased in an imperial (RSB1) or metric version (RSB1/M). For the purpose

of this manual, RSB1(/M) refers to both versions.

To assemble a full Raman spectroscopy system, the RSB1(/M) needs to be equipped with a

front end which collects the scattered Raman signal for detection and analysis, an excitation

light source 785 nm, and a PC.

Note Excitation between 680 nm and 785 nm is possible with your system if your RSBR1(/M)

or RSBC1(/M) was built in 2023 or later. RSBR1(/M) and RSBC1(/M) built in 2021 or 2022 will

require a different bandpass filter for using a shorter wavelength excitation. For this, please

contact techsupport@thorlabs.com. Please be aware that the detection range in wavenumbers

(cm-1) changes when using a different excitation wavelength while the spectral range of the

spectrograph RSB1(/M) remains unchanged.

Thorlabs Front Ends RSBR1(/M) and RSBC1(/M)

Thorlabs offers different front ends for variable applications:

RSBR1(/M) reflective front end for analysis of powder and solid samples:

RSBR1(M) carries the optical modules which guide the laser light to a solid or powder sample,

collect back-scattered Raman signal from the sample and focus it onto the RSB1(/M) input

aperture. For laser light input, the RSBR1(/M) features an FC/PC connector. A sample table is

provided to place a specimen at the optimal focal plane. The RSBR1(/M) even allows to ana-

lyze samples that are transparently wrapped. The wrapping component can be eliminated from

the output spectrum provided that the focal volume is located within the sample.

Raman Spectroscopy Bundle Chapter 1: General Information

RSBC1(/M) cuvette front end for analysis of transparent, translucent, or semi-transparent

materials:

The RSBC1(/M) holds samples in 10 mm cuvettes and collects Raman scattered light in a 90°

geometry with respect to excitation. This is possible for transparent, translucent, or semi-trans-

parent materials. Compared to the reflective front end, due to the 90° geometry, this reduces

the amount of scattered laser light or Rayleigh scattering that enters the spectrograph. Systems

using the RSBC1(/M) can be modified to collect signal at a 180° geometry to accommodate for

polarization dependent Raman spectroscopy. For more information on polarization dependent

Raman Spectroscopy and the modification, please see the application note below.

Both front ends can be purchased as an imperial version (RSBR1, RSBC1) or a metric version

(RSBR1/M, RSBC1/M). For the purpose of this manual, RSBR1(/M) or RSBC1(/M) refers to

both versions, respectively.

RSBF2 fiber input adapter for analysis of fiber coupled Raman scattering

The RSBF2 fiber input adapter is designed for the RSB1(/M) to couple a fiber carrying Raman

scattering from a user setup to the RSB1(/M) base unit. The RSBF2 generates a rectangular fo-

cus of the Raman Signal which matches the rectangular input aperture of the RSB1(/M)

Thorlabs Raman bundle. The RSBF2 is optimized for Raman systems emitting singal from 650

nm to 1050 nm. The RSBF2 is universal for imperial or metric systems.

RSBA1 adapter for 60 mm cage systems attachment to RSB1(/M)

For custom-made solutions, Thorlabs offers the RSBA1 adapter to connect the RSB1(/M) to

Thorlabs 60 mm cage system components. Other custom-made front ends can be connected

to the RSB1(/M) using the SM1.5 thread at the input aperture.

For data acquisition and analysis, Thorlabs provides the OSA Software, Raman Edition

(Thorlabs OSA Raman Software) on the Modular Raman Spectroscopy Kit website. For soft-

ware updates, please see the chapter Software and Installation.

As an excitation light source, Thorlabs recommends the FPV785M laser with the CLD1015

laser driver. The modular design of the Thorlabs Raman bundle also allows use of alternative

lasers, fulfilling the laser requirements stated in this manual.

Attention

Please find all safety information and warnings concerning this product in the chapter Safety in

the Appendix.

Please note that safety of the assembled system is the responsibility of the assembler. This

refers in particular to laser safety for high-power lasers as used for Raman spectroscopy.

1.1 Ordering Codes and Accessories

RSB1

Base Unit for Raman Spectroscopy (Imperial Threads) with Coded Aperture

(CODA Technology) and CMOS-Camera Sensor. Included are a MB1824

Breadboard with BBH1 Lifting Handles, RS2.5P Mounting Posts, CF125C

Clamping Forks, and a USB Stick Carrying the Camera Calibration Data for

Spectra Reconstruction.

Raman Spectroscopy Bundle Chapter 1: General Information

MTN022733-D02

Page 3

RSB1/M

Base Unit for Raman Spectroscopy (Metric Threads) with Coded Aperture

(CODA Technology) and CMOS-Camera Sensor. Included are a

MB4560/M Breadboard with BBH1 Lifting Handles, RS2.5P/M Mounting

Posts, CF125C/M Clamping Forks, and a USB Stick Carrying the Camera

Calibration Data for Spectra Reconstruction.

RSBR1

Front End for Reflective Measurements of Solid Samples with the RSB1

Base Unit (Imperial Type). Included are a Sample Table for Imperial Sys-

tems with a Stray Light Shield and a RPC Polystyrene Calibration Chip.

RSBR1/M

Front End for Reflective Measurements of Solid Samples with the RSB1/M

Base Unit (Metric Type). Included are a Sample Table for Metric Systems

with a Stray Light Shield and a RPC Polystyrene Calibration Chip.

RSBC1

Front End for Measurements of Liquid Samples in a Cuvette with the RSB1

Base Unit (Imperial Type). Included are a TR1.5 Mounting Post and PH2E

Post Holder, a CF125C Clamping Fork, 3500 ml Quartz Cuvette, and a RPB

Polystyrene Calibration Block.

RSBC1/M

Front End for Measurements of Liquid Samples in a Cuvette with the

RSB1/M Base Unit (Metric Type). Included are TR40/M Mounting Post and

PH50E/M Post Holder, a CF125C/M Clamping Fork, 3500 ml Quartz

Cuvette, and a RPB Polystyrene Calibration Block.

RSBF2

Adapter to Feed Fiber-Coupled Raman Signal (650 nm to 1050 nm) into the

RSB1(/M) Base Unit. Input Fiber Requirements: SMA connector; Fiber Dia-

meter < 2.5 mm; NA < 0.5

RSBA1

Adapter for Mounting of a 60 mm Cage System to the Input Aperture of

either RSB1 or RSB1/M.

Required Accessories:

Laser Driver CLD1015 is the Recommended Laser Driver

785 nm Laser We recommend the Thorlabs FPV785M laser. If a different laser is to be

used, please see the recommended laser specifications Technical Data or

contact Thorlabs.

Replacement parts:

RPB Raman Spectroscopy Polystyrene Standard Block for Instrument Calibration

with the RSBC1(/M) Cuvette Measurement Front End.

RPC Raman Spectroscopy Polystyrene Standard Chip (Æ25 mm) for Instrument

Calibration with the RSBR1(/M) Reflective Measurement Front End.

CV10Q35F 3500 ml Macro Fluorescence Cuvette with Cap.

To purchase the above replacement parts, please contact Thorlabs.

Recommended Accessories:

Safety Glasses

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