Zeiss AxioObserver D1 Bedienungsanleitung
ZeissAxioObserver.D1Microscope
Room:3164A
StartUpProcedure
1. Removedustcoverfrommicroscope
2. ToggleFluoArcLampswitchON
3. TogglePowerBarswitchON
4. TurnMicroscopeON
‐bypressingpowerbuttonlocatedonleftsideofmicroscope
5. TurnONcomputer
6. Logonto:ObserverUseraccount(nopassword)
7. OpenProgram:AxioVisionRel.4.8
ShutDownProcedure
1. Ifusedoil,gentlyremoveoilfromobjectiveusingneatlyfolded
drylenspaper
2. Put10xobjectiveinplace,andlowerobjective
3. CloseAxioVisionprogram
4. Shutdowncomputer.Waituntilcompletelyturnedoff
5. TurnMicroscopeOFF
6. TogglePowerBarswitchOFF
7. FluoArcLamp:
‐Regularworkhours:setintensityto50%oncontrolpad(F2),
andleavelampON
‐Non‐workhours:TurnlampOFF
8. Covermicroscopewithdustcover,butDON’TCOVERTHEHOT
LAMPHOUSING!!
Microscope Quick Guide
ZeissAxioObserver.D1MicroscopeGuide
Room:3164A
StartUpProcedure
1. Removedustcoverfrommicroscope
2ToggleFluoArcLampswitchON
3TogglePowerBarswitchON
4TurnMicroscopeON
‐bypressingpowerbuttonlocatedonleftsideofmicroscope
5TurnONcomputer
6Logonto:ObserverUseraccount(nopassword)
7OpenProgram:AxioVisionRel.4.8
ConfiguringImagingSetup
1. Ifnotalreadyvisible,opentheWorkareawindow
2. FromtheWorkareamenu,double‐clickonMultidimensionalAcquisitiontoexpandlist,andthen
selectMultidimensionalAcquisition.
3. Underthetab,clickLoadbutton.Selecttheappropriateexperimentset‐updependingon
whatchannelsyouwanttoimage(ieAllChannels).
4. Selectthecolorstab
5. Youcanactivate/deactivatechannelsbyright‐mouseclickingonthechannelnumber
SetSampleonStageandFocus
Onlyuseslidesthathavebeenthoroughlycleanedofmountingmedium!
1. Choosetheappropriateobjectiveonmicroscope
2. Lightlycleanyourslide(onbothsides)withakimwipeandasmallamountof70%ethanol.
3. Ifusinganoillens,addaverysmalldropofimmersionoil.
4. Loadslideonstage.
5. Bringspecimenintofocusandbringyourareaofinteresttothecenterofthefieldofview.
CapturinganImage
1. Underthetab,selectyourchannelandclicktheMeasurebutton.Alivepreviewwindowappears.
2. Makesurethe‘Lineardisplayoffullrangeofpossiblevalues’buttonisselected.
3. Adjusttheexposuretimetogetanappropriatesignalintensity.ClickOKbuttononleftside.
4. Repeatexposuretimemeasurementforeachchannel,however,adjustthefinefocusforonlyone
channeliethemostimportantone
5. Tocaptureyourfinalimage,pressthebuttonlocatedatthebottomoftheCtab.
Post‐AcquisitionAdjustments
Brightness/Contrast
1. Temporarilyturnoffthepseudo‐coloringofthedisplayedimagebypressingthebutton.Each
channelisdisplayedinblack/white;youcanviewonechannelatatime.
2. Clickonthepropertiesbuttonfoundbelowtheimage
3. WhileunderDisplaytabofpropertieswindow,clicktheMin/Maxbutton,orunderyourimage,press
thebutton.
4. Youcanmakefurtheradjustmentsusingthedisplayedhistogram(iemovetopoflineleft,andbottom
oflineright)
‐NOTE:donotadjusttheGamma
5. Repeatforeachchannel
6. Pressthebutton
AddingaScalebar
1. Toaddascalebar,pressthebutton,orAnnotationsfromthemainmenuandselectScalebar.
2. Dragcursoralongimagetogetdesiredscalebarsize
SavingFiles
1. Fromthemainmenu,selectFileSaveas
2. SaveyourfilesintheAxioVisionformat(.zvi)intheappropriatedestinationyourlabfolderyour
folder.PleaserefertotheAttentionUserssignspostedonthewall.
ShutDownProcedure
8Ifusedoil,gentlyremoveoilfromobjectiveusingneatlyfoldeddrylenspaper
9Put10xobjectiveinplace,andlowerobjective
10 CloseAxioVisionprogram.Donotsavechangesmadetotheexperiment!
11 Shutdowncomputer.Waituntilcompletelyturnedoff
12 TurnMicroscopeOFF
13 TogglePowerBarswitchOFF
14 FluoArcLamp:
‐Regularworkhours:setintensityto50%oncontrolpad(F2),andleavelampON
‐Non‐workhours:TurnlampOFF
15 Covermicroscopewithdustcover,butDON’TCOVERTHEHOTLAMPHOUSING!!
MicroscopeControls
DisplayScreen
TransmittedLightControls
ReflectedLightControls
LightPath
Locatedonleftsideofmicroscope
ObjectiveType
ObjectiveNA
ObjectiveContrastMethod
ObjectiveMagnification
CondenserContrastMethod
HALLampVoltage
TLShutter(open)
RLShutter(closed)
Reflector
Locatedonbottomfrontof
microscope
ControlsHALlamp
voltage/intensity
Dia
p
hra
g
mControl
Condensercontrast
method
Condensera
p
erturecontrol
Locatedonbottomright
ofmicroscope
Opens/closesFLshutter
TurnsHALLampon/off
Locatedonbottomright
ofmicroscope
Locatedatbackofright
focusknob
Movesreflectorturret
50%Camera
50%Ocular
100%Camera 100%Ocular
Objectives
ZeissAxioObserver.D1MicroscopeObjectives
Room:3164A
123
A‐Plan10xLDPlan‐NeoFluar20xPlan‐Apochromat
20x
0.25Ph10.4KorrPh2‐M270.8M27
Magnification10x20x20x
NumericalAperture0.250.40.8
WorkingDistance(mm)4.27.9atcoverglass0.750.55
Coverglass(mm)0.170‐1.50.17
ImmersionNoneNoneNone
LongDistance(LD)‐ Yes‐
CorrectionRing(Korr)‐ Yes‐
OpticalSystemICSICSICS
Flatness***********
ColorCorrection***********
UVTransmission********
IRTransmission*********
DIC‐ ‐ (DICII)*****
PhaseContrastPh1Ph2‐
Catalog#441031‐0000‐000421351‐9970‐710420650‐9901‐00
456
Plan‐NeoFluar
40xPlan‐Apochromat63xPlan‐NeoFluar5x
1.3OilPh31.4OilDICM270.15
Magnification40x63x5x
NumericalAperture1.31.40.15
WorkingDistance(mm)0.20.1913.6
Coverglass(mm)0.170.170.17
ImmersionOilOilair
OpticalSystemICSICSICS
Flatness************
ColorCorrection************
UVTransmission***********
IRTransmission**********
DIC(notequipped)**(DICIII)*****‐
HighContrastDIC‐ Yes‐
PhaseContrastPh3‐ ‐
Catalog#000000‐1022‐819420782‐9900‐000440320‐0000
ICS=InfinityColorCorrrectedSystem
Plan‐NeoFluar100x
1.3OilPh3
100x
1.3
0.2
0.17
Oil
ICS
****
****
****
***
‐
‐
Ph3
000000‐1031‐171
Filter Sets
ZeissAxioObserverD1MicroscopeFilters
Room3164A
1. TransmittedLight
2. FilterSet34(DAPI/BFP)
Excitation:BP390/22
BeamSplitter:FT420
Emission:BP460/50
3. FilterSet38(Cy2/GFP)
Excitation:BP470/40
BeamSplitter:FT495
Emission:BP525/50
4. FilterSet46HE(YFP)
Excitation:BP500/25
BeamSplitter:FT515
Emission:BP535/30
5. FilterSet45(Cy3.5/mcherry)
Excitation:BP560/40
BeamSplitter:FT585
Emission:BP630/75
6. FilterSet50(Cy5)
Excitation:BP640/30
BeamSplitter:FT660
Emission:BP690/50
Filter set
Inhaltsverzeichnis
Andere Zeiss Mikroskop Handbücher
Zeiss
Zeiss EM 10 A Bedienungsanleitung
Zeiss
Zeiss Axiolab 5 Bedienungsanleitung
Zeiss
Zeiss ?IGMA VP-FE-SEM Bedienungsanleitung
Zeiss
Zeiss LSM 710 Bedienungsanleitung
Zeiss
Zeiss LSM 510 Inverted Bedienungsanleitung
Zeiss
Zeiss Axiolab 5 Bedienungsanleitung
Zeiss
Zeiss opmi lumera 700 Bedienungsanleitung
Zeiss
Zeiss Corona extreme Bedienungsanleitung
Zeiss
Zeiss LSM800-6 Bedienungsanleitung
Zeiss
Zeiss OPMI VISU 160 Bedienungsanleitung
Zeiss
Zeiss OPMI PROergo S7 Bedienungsanleitung
Zeiss
Zeiss Axiocam 105 color Bedienungsanleitung
Zeiss
Zeiss TELAVAL 31 Bedienungsanleitung
Zeiss
Zeiss Axioplan 2 imaging and Axiophot 2 Bedienungsanleitung
Zeiss
Zeiss Smartzoom 5 Bedienungsanleitung
Zeiss
Zeiss AxioExaminer FTP-2100 Bedienungsanleitung
Zeiss
Zeiss LSM800-02 Bedienerhandbuch
Zeiss
Zeiss Axioskop 40 Bedienungsanleitung
Zeiss
Zeiss VISU 200 Bedienungsanleitung
Zeiss
Zeiss S100/OPMI pico Bedienungsanleitung
Beliebte Mikroskop Handbücher anderer Marken
Thermo Scientific
Thermo Scientific Nicolet iN10 Leitfaden
National
National DC3-163 Bedienungsanleitung
KERN Optics
KERN Optics OPE-1 Bedienungsanleitung
OPTIKA MICROSCOPES
OPTIKA MICROSCOPES B-150D Bedienungsanleitung
Prior
Prior Lux POL Bedienungsanleitung
Opitec
Opitec Smartscoop 115615 Bedienungsanleitung
VWR
VWR VisiScope 384 Series Bedienungsanleitung
Nikon
Nikon ECLIPSE E200 POL Bedienungsanleitung
Leica
Leica DI C800 Handbuch
ThermoFisher Scientific
ThermoFisher Scientific Continuµm Bedienungsanleitung
ThermoFisher Scientific
ThermoFisher Scientific Continuµm Bedienungsanleitung
Olympus
Olympus SZ61 Bedienungsanleitung