Agilent Technologies 1260 Infinity Bedienungsanleitung

Agilent Technologies

Agilent 1260 Infinity

Isocratic Pump and

Quaternary Pump

User Manual

Agilent 1260 IsoPump/QuatPump User Manual

Notices

No part of this manual may be reproduced

in any form or by any means (including elec-

tronic storage and retrieval or translation

into a foreign language) without prior agree-

ment and written consent from Agilent

Technologies, Inc. as governed by United

States and international copyright laws.

Manual Part Number

G1310-90012

Edition

06/10

Printed in Germany

Agilent Technologies

Hewlett-Packard-Strasse 8

76337 Waldbronn

This product may be used as a com-

ponent of an in vitro diagnostic sys-

tem if the system is registered with

the appropriate authorities and com-

plies with the relevant regulations.

Otherwise, it is intended only for gen-

eral laboratory use.

Warranty

The material contained in this docu-

ment is provided “as is,” and is sub-

ject to being changed, without notice,

in future editions. Further, to the max-

imum extent permitted by applicable

law, Agilent disclaims all warranties,

either express or implied, with regard

to this manual and any information

contained herein, including but not

limited to the implied warranties of

merchantability and fitness for a par-

ticular purpose. Agilent shall not be

liable for errors or for incidental or

consequential damages in connection

with the furnishing, use, or perfor-

mance of this document or of any

information contained herein. Should

Agilent and the user have a separate

written agreement with warranty

terms covering the material in this

document that conflict with these

terms, the warranty terms in the sep-

arate agreement shall control.

Technology Licenses

The hardware and/or software described in

this document are furnished under a license

and may be used or copied only in accor-

dance with the terms of such license.

Restricted Rights Legend

If software is for use in the performance of a

U.S. Government prime contract or subcon-

tract, Software is delivered and licensed as

“Commercial computer software” as

defined in DFAR 252.227-7014 (June 1995),

or as a “commercial item” as defined in FAR

2.101(a) or as “Restricted computer soft-

ware” as defined in FAR 52.227-19 (June

1987) or any equivalent agency regulation

or contract clause. Use, duplication or dis-

closure of Software is subject to Agilent

Technologies’ standard commercial license

terms, and non-DOD Departments and

Agencies of the U.S. Government will

receive no greater than Restricted Rights as

defined in FAR 52.227-19(c)(1-2) (June

1987). U.S. Government users will receive

no greater than Limited Rights as defined in

FAR 52.227-14 (June 1987) or DFAR

252.227-7015 (b)(2) (November 1995), as

applicable in any technical data.

Safety Notices

CAUTION

A CAUTION notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly per-

formed or adhered to, could

result in damage to the product

or loss of important data. Do not

proceed beyond a CAUTION

notice until the indicated condi-

tions are fully understood and

met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice,

or the like that, if not correctly

performed or adhered to, could

result in personal injury or

death. Do not proceed beyond a

WARNING notice until the indi-

cated conditions are fully under-

stood and met.

Agilent 1260 IsoPump/QuatPump User Manual 3

Contents

1 Introduction 7

Introduction to the Pump 8

Overview of the Hydraulic Path 10

Early Maintenance Feetback 17

Instrument Layout 18

Electrical Connections 19

Interfaces 21

Setting the 8-bit Configuration Switch 27

2 Site Requirements and Specifications 35

Site Requirements 36

Physical Specifications 39

Performance Specifications 40

3 Installing the Pump 43

Unpacking the Pump 44

Optimizing the Stack Configuration 47

Installing the Pump 50

Connecting Modules and Control Software 53

Flow Connections of the Pump 56

Priming the System 59

4UsingthePump 65

Hints for Successful Use of the Isocratic Pump 66

Prevent Blocking of Solvent Filters 68

Algae Growth in HPLC Systems 69

5 Optimiziming Performance 71

When to Use a Vacuum Degasser 72

Operational Hints for the Multi Channel Gradient Valve (MCGV) 73

When to use the Seal Wash Option 74

Choosing the Right Pump Seals 76

Optimize the Compressibility Compensation Setting 77

4 Agilent 1260 IsoPump/QuatPump User Manual

Contents

6 Troubleshooting and Diagnostics 79

Overview of the Module’s Indicators and Test Functions 80

Status Indicators 82

User Interfaces 84

Agilent Lab Advisor Software 85

7 Error Information 87

What Are Error Messages 89

General Error Messages 90

Module Error Messages 96

8 Test Functions and Calibration 111

Introduction 112

System Pressure Test 113

Leak Rate Test 114

9 Maintenance 115

Introduction to Maintenance and Repair 116

Warnings and Cautions 117

Overview of Maintenance 119

Cleaning the Module 120

Checking and Cleaning the Solvent Filter 121

Exchanging the Passive Inlet Valve (PIV) 123

Exchanging the Outlet Valve 124

Exchanging the Purge Valve Frit or the Purge Valve 126

Removing the Pump Head Assembly 128

Maintenance of a Pump Head Without Seal Wash Option 130

Seal Wear-in Procedure 134

Maintenance of a Pump Head with Seal Wash Option 135

Reinstalling the Pump Head Assembly 138

Exchanging the Multi-Channel Gradient Valve (MCGV) 140

Exchanging the Optional Interface Board 143

Exchanging the Active Inlet Valve (AIV) or its Cartridge 145

Replacing the Module’s Firmware 147

Agilent 1260 IsoPump/QuatPump User Manual 5

Contents

10 Parts for Maintenance 149

Pump Head Assembly Without Seal Wash 150

Pump Head Assembly with Seal Wash Option (600 bar) 152

Outlet Valve 154

Purge Valve Assembly 155

Active Inlet Valve Assembly 156

Accessory Kit G1311-68755 157

Seal Wash Option Kit 158

Solvent Cabinet 159

Bottle Head Assembly 161

Hydraulic Path 162

11 Identifying Cables 165

Cable Overview 166

Analog Cables 168

Remote Cables 170

BCD Cables 173

CAN/LAN Cables 175

External Contact Cable 176

Agilent Module to PC 177

Agilent 1200 Module to Printer 178

12 Appendix 179

General Safety Information 180

The Waste Electrical and Electronic Equipment Directive 183

Batteries Information 184

Radio Interference 185

Sound Emission 186

Solvent Information 187

Agilent Technologies on Internet 188

6 Agilent 1260 IsoPump/QuatPump User Manual

Contents

Agilent 1260 IsoPump/QuatPump User Manual

Agilent Technologies

Introduction

Introduction to the Pump 8

Overview of the Hydraulic Path 10

Hydraulic Path 11

How Does the Pump Work? 12

How Does Compressibility Compensation Work? 15

How Does Variable Stroke Volume Work? 16

Early Maintenance Feetback 17

Instrument Layout 18

Electrical Connections 19

Serial Number Information (ALL) 20

Rear View of the Module 20

Interfaces 21

Overview Interfaces 23

Setting the 8-bit Configuration Switch 27

Communication Settings for RS-232C 30

Special Settings 32

This chapter gives an introduction to the module, instrument overview and

internal connectors

8 Agilent 1260 IsoPump/QuatPump User Manual

1Introduction

Introduction to the Pump

Introduction to the Quaternary Pump

The quaternary pump comprises a solvent cabinet, a vacuum degasser and a

four-channel gradient pump. The four-channel gradient pump comprises a

high-speed proportioning valve and a pump assembly. It provides gradient

generation by low pressure mixing. A solvent cabinet provides enough space

for four one-liter bottles. An active seal wash (optional) is available when the

quaternary pump is used with concentrated buffer solutions.

Figure 1 Overview of the Quaternary Pump

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Agilent 1260 IsoPump/QuatPump User Manual 9

Introduction 1

Introduction to the Pump

Introduction to the Isocratic Pump

The isocratic pump has the same operating principle as the quaternary pump

but has only one solvent channel, that means the composition cannot be

changed during a method because there is no multi-channel gradient valve

(MCGV). The isocratic pump does not include a degasser. An upgrade product

(Isocratic to Quaternary Pump Upgrade Kit (p/n G4207-60000)) is available

for upgrading the isocratic pump to a quaternary pump including a degasser.

Figure 2 Overview of the Isocratic Pump

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10 Agilent 1260 IsoPump/QuatPump User Manual

1Introduction

Overview of the Hydraulic Path

Both the isocratic pump and quaternary pump are based on a two-channel,

dual-plunger in-series design which comprises all essential functions that a

solvent delivery system has to fulfill. Metering of solvent and delivery to the

high-pressure side are performed by one pump assembly which can generate

pressure up to 600 bar.

In the quaternary pump, degassing of the solvents is done in a built-in vacuum

degasser. Solvent compositions are generated on the low-pressure side by a

high-speed proportioning valve (MCGV).

The pump assembly includes a pump head with a passive inlet valve and an

outlet valve. A damping unit is connected between the two piston chambers. A

purge valve including a PTFE frit is fitted at the pump outlet for convenient

priming of the pump head.

An active seal wash (optional) is available for applications using concentrated

buffers as solvents.

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