Endress+Hauser Analytik Jena UVP Transilluminator Bedienungsanleitung

UVP Transilluminators

Instruction Manual

Analytik Jena US Ultra-Violet Products Ltd.

2066 W 11th Street, Upland, CA 91786 Unit 1,Trinity Hall Farm Estate,

Tel: (909) 946-3197 Nuffield Road Cambridge CB4 1TG UK

Fax: (909) 946-3597 Tel: +44(0)1223-420022 / Fax: +44(0)1223-420561

Web site: www.us.analytik-jena.com

81-0249-01 Rev H

UVP Transilluminators Page 2

81-0249-01 Rev H

Introduction

The UVP Transilluminator offers the researcher uniform and intense sources of ultraviolet light

(radiation) in a compact package. The special design emits high intensity excitation UV wavelength for

back-illumination of transparent fluorescent materials. The 302nm UV back-illumination provides a

highly sensitive method to detect double-stranded nucleic acids that have been labeled with fluorescent

dyes such as ethidium bromide or acridine orange. Single stranded nucleic acids may be detected, but

with a lower excitation wavelength more sensitive for nucleic acid visualization than the 365nm model.

The transilluminator is uniquely designed with increased UV intensity and uniformity, instant on

capabilities, no lamp flicker and reduced electrical consumption.

NOTE: Though the manual refers to the midrange UV waveband as 302nm, others refer to this region

as 300nm or 312nm. The spectral output of all these regions is the same.

A Word of Caution: UV Transilluminators are powerful sources of UV radiation that will cause damage

to unprotected eyes and skin. Before operating any unit, be sure all personnel in the area are properly

protected. If not using the transilluminator with an imaging system darkroom, a UV Blocking Cover

should be attached to the transilluminator. Even though this cover blocks the ultraviolet radiation

emitted by the unit, UV Blocking Eyewear should be worn as well.

UVP Transilluminator features:

UV Blocking Cover may be

included with the

transilluminator. The cover is

hinged and adjustable to

varying heights.

Select models include a

control knob for selection of

wavelengths or intensity

Power switch

AJ’s long life filters are

available in a variety of

filter sizes from 15 x

15cm to 25 x 26cm

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Specifications

UVP Transilluminators

Single UV UVP Transilluminator models are designed with either single intensity or variable intensity.

Units are equipped with an electronic ballast.

Dimension: Width: 14” x Depth: 11” x Height: 4.8” (356 x 279 x 122mm)

No. of Intensity

Model Part Number Volt/Hz Wavelength Filter Size Tubes Style

M-15 95-0455-01 100-115/60 302nm 15 x 15cm 4 x 8W Single

95-0455-02 230/50

M-15V 95-0456-01 100-115/60 302nm 15 x 15cm 4 x 8W Variable

95-0456-02 230/50

M-20 95-0447-01 100-115/60 302nm 20 x 20cm 4 x 8W Single

95-0447-02 230/50

M-20V 95-0452-01 100-115/60 302nm 20 x 20cm 4 x 8W Variable

95-0452-02 230/50

M-26 95-0457-01 100-115/60 302nm 21 x 26cm 4 x 8W Single

95-0457-02 230/50

M-26V 95-0458-01 100-115/60 302nm 21 x 26cm 4 x 8W Variable

95-0458-02 230/50

M-26XV 95-0413-01 100-115/60 302nm 25 x 26cm 4 x 8W Variable

95-0413-02 230/50

The mini single UV UVP Transilluminator uses a magnetic core/coil design ballast. The transilluminator

dimensions are: 7.25”D x 10.25”W x 4.5”H. This model has a single intensity.

Model Part Number Volt/Hz/Amp Wavelength Filter Size No. of Tubes

M-10E 95-0180-01 115/60/0.7 302nm 10 x 10cm 5 x 6W

95-0180-02 230/50/0.6

95-0180-03 100/50-60/0.8

UVP 2UV Transilluminators

Units are equipped with an electronic ballast. This model has a single intensity. The physical dimensions

of all models are:

Width: 14” x Depth: 11” x Height: 4.8” (356 x 279 x 122mm)

Model Part Number Volt/Hz Wavelength Filter Size #of Tubes/UV

LM-20 95-0449-01 100-115/60 302/365nm 20 x 20cm 4 x 8W/302nm

95-0449-02 230/50 4 x 8W/365nm

LM-26 95-0459-01 100-115/60 302/365nm 21 x 26cm 4 x 8W/302nm

95-0459-02 230/50 4 x 8W/365nm

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UVP 3UV Transilluminators

Units are equipped with an electronic ballast. The physical dimensions of all models are:

Width: 14” x Depth: 11” x Height: 5.4” (356 x 279 x 137mm)

Model Part Number Volt/Hz Wavelength Filter Size No. of Tubes/UV

LMS-20 95-0417-01 115/60 302/365/254nm 20 x 20cm

LMS-20 95-0417-02 230/50

LMS-26 95-0414-01 115/60 302/365/254nm 21 x 26cm

LMS-26 95-0414-02 230/50

Transilluminator Operation

Safety Precautions

When the UV Blocking Cover is not being

used, UV light may escape through the

holes dedicated to accepting the bracket

pins of the UV Blocking Cover.

Remove the black safety plugs from

their package

Insert the safety plugs through the

holes as shown.

Set-Up

Place the transilluminator on a level work surface. Be sure that an air space exists around the

bottom of the work surface. This space allows for the proper air circulation through the unit.

Plug the female end of the power cord into the transilluminator. For 230 volt models, or those

requiring special power cord connectors, ensure that the proper configuration of male

connector or plug has been properly connected to the power cord.

Plug the male end of the power cord into a properly grounded electrical outlet. The proper

voltage of the transilluminator is found on the product information label. If using the

transilluminator with an imaging system, a jumper cable is required for connecting to the

darkroom. Refer to the imaging system documentation for additional instructions.

The transilluminator may be equipped with a UV Blocking Cover. This cover allows the user to

safely view and work with the gel/sample on the glass surface while the transilluminator is on

(be sure to wear protective gloves when working with the gel/sample during this process). To

install, remove the brown protective paper from the cover. Insert the bracket pins on the cover

into the holes on the front of the transilluminator.

Safety Plug

4 x 8W/302nm

4 x 8W/365nm

4 x 8W/254nm

4 x 8W/302nm

4 x 8W/365nm

4 x 8W/254nm

UVP Transilluminators Page 5

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If you are not using the transilluminator with an imaging system darkroom, do not operate the unit

without securing the cover. If the cover is missing, a UV Blocking Faceshield must be worn to avoid

UV exposure to the skin. UV Blocking Eyewear should be worn even with the cover in place to avoid

accidental UV exposure.

Using the Transilluminator

Place gel/sample on the filter area. It is recommended to place the gels on a Gel-Tray to protect the

filter surface from cuts and scratches. It is recommended that gloves be worn to avoid contact with gel

and staining agents.

Press the ON/OFF switch to ON. The UV tubes within the unit should be glowing beneath the filter. If

using the transilluminator with an imaging system, the system’s main power is required to be in the ON

position.

When using a transilluminator with multiple UV wavelengths, dial the knob to the appropriate

wavelength setting.

When using the Variable Intensity models, use the variable intensity settings as follows:

High: allows for UV excitation of fluorophores on gels for routine photography and for

excitation of gels with low sample concentration

Medium: Excellent for viewing and quick single-band excision

Low: Allows for positioning and preparation of gels, excising multiple bands and focusing for

photography

The UV Blocking Cover, optionally installed during setup, is adjustable to varying angles to provide

easier access to the filter surface. To adjust the angle, rotate the Phillips head screws (located on the

left sides of the cover’s hinges) clockwise until enough tension has been placed on the hinge to hold the

cover at the desired angle.

After viewing/photographing the sample, turn the transilluminator off.

Permanently Installing the Safety Cover

(Optional)

Your UVP transilluminator includes a UV-blocking safety shield which can be installed temporarily (as

described elsewhere in this manual) or permanently, if desired. The following instructions will explain

the process of permanently installing the UVP-supplied UV blocking cover onto the UVP

transilluminator.

To complete this procedure, the following tools and parts will be required:

Phillips-head screwdriver

5/16” nut driver

3/8” narrow walled nut driver

(4) hollow 3/8” nuts (supplied with safety cover)

CAUTION: This procedure requires a moderate level of technical competence. If you are not comfortable

working with electronics, tools and/or related components, contact Analytik Jena for assistance.

Note:

The transilluminator images shown in this procedure may differ in appearance from your

transilluminator. However, the procedure is the same.

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Removal Procedure

When performing the following procedure, place all components (screws, nuts, etc.) in a secure

location, as some will be reused for installation.

1. Turn off the transilluminator by placing the power switch on the front of the unit in the OFF

position. Then, unplug the unit from the wall power.

2. Remove the four Phillips-head screws and washers securing the transilluminator filter frame to the

body of the transilluminator. Lifting up, remove the transilluminator filter frame from the

transilluminator body and place it upside-down on a flat, smooth surface to avoid scratching the filter

glass.

3. Locate the two sets of lock nuts and three metal spacers on the inside of the transilluminator filter

frame, as shown in the image below. Use the 5/16” nut driver to remove the two sets of lock nuts and

spacers.

Two of Four

Phillips-Head

Screws and

Washers

Transilluminator Filter

Frame with Cover

Transilluminator

Body

Lock Nuts

Metal Spacers

UVP Transilluminators Page 7

81-0249-01 Rev H

4. Lift the transilluminator filter frame and place the UV blocking cover below the frame. Insert the

black tabs, located on the UV blocking cover’s hinges, through the filter frame as shown.

5. Use the narrow walled 3/8” nut driver to secure the four supplied 3/8” hollow nuts to the black UV

blocking cover hinge tabs.

Installation Procedure

1. With the UV blocking cover hinges at the front of the unit, replace the transilluminator filter frame

on top of the body of the transilluminator.

Black Tabs

UV Blocking

Cover Hinges

UVP Transilluminators Page 8

81-0249-01 Rev H

2. Reinstall the four Phillips-head screws and washers on the sides of the transilluminator to secure

the transilluminator filter frame to the transilluminator body.

Cleaning and Care of the Transilluminator

Clean unit surface with a damp soft cloth or sponge. Never use abrasive cleaners (can damage the UV

filter surface).

To protect the filter glass and minimize moisture and liquids on the glass, it is recommended that you

use a UV transmitting Gel-Tray. Refer to the Replacement Parts for ordering information.

Replacing Tubes in the Transilluminator

Disconnect the transilluminator for the electrical supply.

Remove the filter cover: Use a Phillips head screwdriver to remove the four screws on the sides of the

unit. Lift the filter cover off the unit.

Remove the reflectors on the left and right side of the unit: Slide the reflectors up out of the unit.

Remove the tube: Carefully rotate the tube and slide out of the socket. Replace with a new tube by

sliding the tube into the socket and rotating into place.

Insert the reflectors back into place and reattach the filter cover.

Phillips-Head

Screw and

Washer

UV Blocking

Cover

Transilluminator

Body

UVP Transilluminators Page 9

81-0249-01 Rev H

Maintenance/Repair/Technical Assistance

Replacement Parts/Accessories

For replacement parts or components not shown here, contact Analytik Jena Customer Service or place

of purchase. Please have the transilluminator model number available when you call.

Replacement Part Description Part Number Qty. Required

UVP Filter Assembly (M-10) 38-0150-01 1

Filter Assembly (M-15) 38-0313-03 1

Filter Assembly (M-15V) 38-0313-09 1

UVP Filter Assembly (M-20) 38-0313-01 1

UVP Filter Assembly (M-26) 38-0313-02 1

Filter Assembly (M-20V, LM-20, LMS-20) 38-0313-07 1

Filter Assembly (M-26V, LM-26, LMS-26) 38-0313-08 1

Filter Assembly (M-26XV) 38-0163-04 1

UVP Cover, UV blocking (M-10E) 19-0114-01 1

UVP Cover, UV blocking (all other models) 19-0121-01 1

UV Tube, 6 Watt, 302nm midrange (FL6E) –TM-10E 34-0044-01 5

UV Tube, 8 Watt, 302nm midrange (FL8E) 34-0042-01 4

Tube, 8 Watt, 365nm longwave UV (F8T5/BL) 34-0006-01 4

Tube, 8 Watt, 254nm shortwave UV 34-0007-01 4

Accessories Description Part Number

UVP Gel-Cutter 85-0002-01

UVP Gel-Scooper 85-0006-01

UVP Gel-Tray, UV Transmitting, Sm. 11.5”W x 9”D (29 x 23cm) 85-0007-01

UVP Gel-Tray, UV Transmitting, Lg. 16.5”W x 10.5”D (42 x 27cm) 85-0005-01

UVP Gel-Tray, UV Transmitting (25 x 26cm) 38-0296-03

UVP Gel-Ruler, UV Fluorescing 85-0003-01

UVP Spectacles, UV Blocking 98-0002-01

UVP Goggles, UV Blocking 98-0002-02

UVP Faceshield, UV Blocking 98-0002-04

UVP Transilluminators Page 10

81-0249-01 Rev H

Technical Support

Analytik Jena offers technical support for all of its products. If you have any questions about the

product’s use or, operation, please contact AJ’s offices at the following locations.

If you are in North America, South America, East

Asia or Australia:

If you are in Europe, Africa, the Middle East,

Western Asia:

Call (909) 946-3197

Customer Service regular business days, between

7am and 5pm PST

Call +44(0)1223-420022 Customer regular

business days, between 9am and 5:30pm

E-Mail: [email protected]

Fax: Customer Service: (909) 946-3597

Fax: Customer Service: +44(0)1223-420561

Write to: Analytik Jena US

2066 W. 11th Street

Upland, CA 91786 USA

Write to: Ultra-Violet Products Ltd.

Unit 1, Trinity Hall Farm Est, Nuffield Rd

Cambridge CB4 1TG UK

Note: A Returned Goods Authorization (RGA) number must be obtained from AJ Customer Service

before returning any product.

Warranty

Analytik Jena warrants its Ultraviolet Transilluminators to be free of detects in materials and workmanship

for a period of two (2) years from date of purchase. The foregoing warranty of AJ shall be of no force and

effect if buyer has modified or damaged the product. Tubes and filters are warranted for 90 days.

All warranties or merchantability and fitness for any purpose and all other warranties, expressed or implied,

except those expressly set forth herein, are deemed waived and excluded.

AJ’s duty under the warranty is limited to replacement and/or repair of the defective part at the option of AJ.

AJ shall not be liable for any expenses or damages incurred by the purchaser except as expressly set forth

herein, and in no event shall AJ be liable for any special, incidental or consequential damages of any kind.

This warranty does not supersede any statutory rights that may be available in certain countries.

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