Endress+Hauser Analytik Jena UVP DigiDoc-It Leitfaden

81-0218-01 Rev P

UVP DigiDoc-It®Imaging System

Installation and User Instructions

Analytik Jena US Analytik Jena AG

2066 W. 11th Street Konrad-Zuse-Str. 1

Upland, CA 91786 07745 Jena, Germany

Phone: (909) 946-3197 Phone: + 49 3641 77 7407

Fax: (909) 946-3597 Fax: +49 3641 77 7449

Web Site: us.analytik-jena.com

UVP DigiDoc-It Imaging System 2

81-0218-01 Rev P

Introduction

The UVP DigiDoc-It Imaging System is a basic gel imaging system designed to fit over any of Analytik Jena’s optional

UVP Transilluminator PLUS or optional UVP Transilluminator and drawer combination. The system combines:

Hood enclosure

DigiCam 125 digital color camera

Ethidium Bromide (EtBr) Filter

VisionWorks®Acquisition and Analysis Software (refer to software manual for installation)

UV transilluminator or drawer assembly (optional). Transilluminator instructions are provided in a

separate manual.

Hood Enclosure

The DigiDoc-It hood enclosure includes a cap to reduce ambient and

reflective light when taking pictures. The soft rubber viewport window

allows researchers to view samples prior to capturing images. The slide

closure under the viewport minimizes ambient light when taking pictures.

The lightweight door provides access to the enclosure interior for

placement of samples on the transilluminator surface.

The DigiDoc-It drawer fits under the hood enclosure and accommodates a compact UV Transilluminator.

Camera cap

enclosure for access

to the camera

Handles for carrying

the hood

Gel Viewport

Window

Hood access door

DigiCam 125 camera

Ethidium Bromide Filter is

located inside the hood

under the camera

Viewport cover

UVP Transilluminator

PLUS

DigiDoc-It drawer

(Note: color scheme is different)

UVP DigiDoc-It Imaging System 3

81-0218-01 Rev P

Camera & UV Filter

The digital color camera includes a zoom lens.

The EtBr filter, orange-colored UV blocking band pass filter, is installed inside the hood at the Analytik Jena factory.

The filter is used to absorb UV and IR radiation from the transilluminator and to enhance the orange/pink bands

generated by Ethidium Bromide stained gels. The filter can be removed when imaging non-fluorescent media

(protein gels, colony plates, etc.) in order to produce brighter images.

VisionWorks® Software

The VisionWorks® software loads onto the user’s existing computer. The software controls the camera acquisition

functions as well as enhancement and reporting features. For information on installation and use of the software,

refer to the VisionWorks Software help files.

UV Transilluminator

A UV transilluminator may be shipped with the system. The transilluminator includes an ultraviolet blocking cover and

the cover should be removed when the transilluminator is used with the system. Refer to the Transilluminator manual

for separate instructions.

Operating System Requirements

Windows 7, 8, 10

Intel Pentium Processor or equivalent, 1.6 GHz or higher

1 GB of RAM or greater

200 MB of available hard disk space for the program, more for data

One Universal Serial Bus (USB) for connecting the camera

Color monitor, supporting at least 1024 x 768 resolution and 16-bit or better colors; 24-bit or 32-bit color is

strongly recommended

NOTE: For 21 CFR Part 11 support functionality, the computer partition must be formatted with NTFS. Refer to the

VisionWorks® software manual for instructions.

UVP DigiDoc-It Imaging System 4

81-0218-01 Rev P

UVP Transilluminator PLUS

(shown with UV blocking cover)

DigiDoc-It Drawer

(Note: different color scheme and style)

System Setup

DO NOT ATTEMPT TO CONNECT ANY WIRING WHILE THE EQUIPMENT IS CONNECTED TO ANY POWER SUPPLY.

CAUTION: Do not install the system in places with high moisture, dust or high temperature. Do not use any oil or

petroleum based cleaner for the cabinet. Use only mild soap or detergent solution for cleaning. Ensure that the

system is turned OFF during cleaning. Keep the equipment away from motors or other large magnetic equipment

apparatus.

Place the Hood on top of the Transilluminator

The Hood Enclosure can be used with a UVP Transilluminator PLUS. When using a UVP Transilluminator, the optional

drawer is required.

CAUTION: All Analytik Jena Transilluminators are powerful sources of UV radiation that will cause damage to

unprotected eyes and skin. Before operating any unit, be sure all personnel in the area are properly protected. UV

Blocking Eyewear is highly recommended and should be worn. Refer to the Accessories section of this manual for UV

Blocking Eyewear.

UVP Transilluminator PLUS

1. Remove the UV blocking clear cover from the Transilluminator.

2. Position the transilluminator so the switch is facing the user.

3. Connect the power cord to the back of the transilluminator and

connect the plug to a power source.

4. Place the Hood Enclosure on top of the Transilluminator surface so

that the filter glass is covered.

UVP Transilluminator and Drawer

1. Remove the UV blocking clear cover from the Transilluminator.

2. Place the Transilluminator into the drawer.

3. Connect the power cord to the back of the transilluminator and

push the plug through the access hole in the back of the drawer.

4. Connect the plug to the power source.

5. Access to the transilluminator switch is via the access port on the

front of the drawer.

6. Place the DigiDoc-it hood over the drawer.

Camera Assembly

1. The camera will be installed in the DigiDoc-It hood at the Analytik

Jena factory.

2. To connect the power cable to the camera, thread the camera’s

power cable through the round notched hole from the back of the

camera cap and plug it into the camera as shown.

3. To connect the USB cable to the camera, thread the camera’s USB cable through the round notched hole

from the back of the camera cap and plug into the camera. Leave the other end unplugged until ready to

load the software.

4. Plug the camera’s power supply into a wall outlet or surge-protector receptacle.

UVP DigiDoc-It Imaging System 5

81-0218-01 Rev P

Note: The Ethidium Bromide filter is installed inside the hood enclosure at the Analytik Jena factory. If the filter

needs to be replaced, turn the hood upside down. Loosen screws and slide the filter into the filter tray. Retighten

screws and place the hood upright.

Install the VisionWorks® Software

NOTE: The following provides a brief instruction for installing the software. For additional instruction on using the

software, refer to the VisionWorks help files.

1. Insert the VisionWorks flash drive into the computer.

2. Navigate to the flash drive folder and open the folder.

3. Open the first folder found in the drive and then open the Installation folder.

4. Scroll to find the SetupVisionWorks.exe file and double click to run installation.

5. The first window will be a welcome to prerequisites screen. Click OK, Next. Leave all options in their

default settings and click Next. Proceed through the prerequisite driver installations. Once complete,

VisionWorks® installer will open, where the user can click INSTALL and proceed until complete.

Connect the Camera to the Computer

NOTE: It is recommended to only connect the camera to the computer while the camera is off.

1. Connect the USB cable from the camera to the computer.

2. Turn the camera on and select “M” on the camera dial.

NOTE: Do not use the VisionWorks® software while the camera dial is set to movie mode as doing

so may result in an error.

Hole in DigiDoc-It

hood for cables

Power supply cable,

plug into wall outlet

USB cable, plug into

computer

DigiCam 125 camera

(may appear different

than shown)

UVP DigiDoc-It Imaging System 6

81-0218-01 Rev P

Set camera to M

Connect to VisionWorks® Software

1. Open the VisionWorks software. The software should automatically identify the camera. The camera

control window should be active and ready to capture images.

UVP DigiDoc-It Imaging System 7

81-0218-01 Rev P

System Operation

Loading Gels into the Darkroom Hood

1. Turn Off the UV Transilluminator.

2. Open the darkroom door.

3. Place the sample to be imaged in the center of the transilluminator filter glass.

4. Close the darkroom door.

5. Turn On the transilluminator.

Note: It is important to minimize the light source exposure to the sample. Turn off the light source as soon as the

image is captured.

View a Sample Prior to Capture

1. Open the Viewport Window on the hood by sliding the cover to the right.

2. After visually checking that the sample is loaded correctly, close the Viewport Window. Otherwise, light from

the viewport will interfere with the capturing process.

Camera Operation

NOTE: Do not use the VisionWorks® software while the camera dial is set to movie mode as doing so may

result in an error.

1. To use the camera, open the camera cap on top of the hood.

2. To capture in automatic mode, set the camera mode dial button to A-DEP. (Automatic mode is

recommended for a quick image capture)

3. Close and secure the camera cap on the hood.

Note: If the camera is off when starting VisionWorks® software, a message stating that the application

cannot find a camera will appear. To remedy this, turn the camera on and click Retry.

Software Operation

NOTE: Do not use the VisionWorks® software while the camera dial is set to movie mode, as doing so may

result in an error.

1. Ensure that the camera mode is set to A-DEP for automated capture.

2. Open the VisionWorks® software.

3. A window displaying the camera options should appear as pictured below. If not, click on the

Acquisition Action tab and then on the Camera menu button.

UVP DigiDoc-It Imaging System 8

81-0218-01 Rev P

Camera Control Window

4. Select Preview at the top of the software to preview the image.

5. While in preview mode, the Brightness slider can be adjusted to brighten dark images.

6. The user may adjust the focus manually to enhance the appearance of the previewed image. Click the

Manual box on the Preview window, then adjust the focus settings.

Preview Window with Auto Focus

7. When satisfied with the previewed image, select Capture at the top of the software.

UVP DigiDoc-It Imaging System 9

81-0218-01 Rev P

Optional Camera Setup (Image Acquisition)

If operating the camera in automatic mode is not sufficient for the application, try utilizing the manual mode to gain

additional camera control.

1. Access the camera and turn the camera dial to M, the next position on the dial.

Set camera to M

2. A new camera window appears with additional options:

Camera Control in Manual Mode

3. Select the desired options for an enhanced image. See the camera manual for detailed descriptions of

options.

4. Select Capture at the top of the software.

UVP DigiDoc-It Imaging System 10

81-0218-01 Rev P

Service Procedures

Return Procedure

A Returned Goods Authorization (RGA) number must be obtained from Analytik Jena Customer Service

before returning any product.

Replacement Parts and Accessories

Accessories are available for use with the DigiDoc-It Imaging Systems. Contact Analytik Jena’s offices at the

telephone numbers below for replacement parts for DigiDoc-It equipment. For transilluminator replacement

parts, refer to the separate manual for the transilluminators included with your shipment.

Description Part Numbers

UVP Digidoc-it Drawer (for UVP Transilluminators) 98-0068-01

VisionWorks® Acquisition and Analysis Software 81-0266-01

UVP Thermal Printer, Digital (115/230V) 89-0069-06

UVP Thermal Printer, Digital (230V) 89-0069-07

Thermal Printer, Digital (230V for Europe) 98-0069-15

UVP Thermal Paper, 4 rolls (800 images) 89-0038-01

Thermal paper, 16 rolls (3200 images) 89-0038-04

Thermal Paper, 40 rolls (8000 images) 89-0038-05

UVP Visi-White™ Converter Plate (21x26cm) 38-0191-01

UVP Visi-White™ Converter Plate (20x40cm) 38-0191-02

UVP Visi-Blue™Converter Plate (21x26cm) 38-0200-01

UVP Visi-Blue™Converter Plate (20x40cm) 38-0200-02

UVP Filter, 50mm, sq., SYBR Green and EGFP 38-0219-01

UVP Filter, 50mm, sq., Ethidium Bromide 38-0220-01

Technical Assistance

Analytik Jena offers technical support on all of its products. If there are questions about the product’s use,

operation or repair, please contact our offices at the locations below, or go to Analytik Jena’s web site and

click the Tech Support > BioImaging Systems.

If residing in North America, South

America, East Asia or Australia:

If residing in Europe, Africa, the Middle East

of Western Asia:

Call (909) 946-3197, and ask for Customer

Service during regular business days,

between 7:00 am and 5:00 pm, PST.

Call +44(0) 1223-42002, and ask for Customer

Service during regular business days between

9:00 am and 5:30 pm.

E-mail your message to:

[email protected]

E-mail your message to: [email protected]

Fax Customer Service, and send it to (909)

946-3597

Fax Customer Service, and send it to: +44(0)

1223-420561

Write to: Analytik Jena 2066 W. 11th Street,

Upland, CA 91786 USA

Write to: Ultra-Violet Products Ltd

Unit 1, Trinity Hall Farm Estate, Nuffield Road,

Cambridge CB4 1TG UK

Inhaltsverzeichnis

Andere Endress+Hauser Laborausrüstung Handbücher

Endress+Hauser

Endress+Hauser Liquiphant FTL51B Firmware-Update

Endress+Hauser

Endress+Hauser Analytik Jena Biometra TRIO Bedienungsanleitung

Endress+Hauser

Endress+Hauser analytikjena CyBio QuadPrint HQ Bedienungsanleitung

Endress+Hauser

Endress+Hauser Liquistation CSF34 Technische Daten

Endress+Hauser

Endress+Hauser ISEmax CAM40 Bedienungsanleitung

Endress+Hauser

Endress+Hauser Analytik Jena Biometra TOne Anleitung zur Verwendung

Endress+Hauser

Endress+Hauser OXY5500 QF Firmware-Update

Endress+Hauser

Endress+Hauser analytikjena qTOWER3auto Series Bedienungsanleitung

Endress+Hauser

Endress+Hauser Liquiline Control CDC90 Bedienungsanleitung

Endress+Hauser

Endress+Hauser Liquistation CSF48 Gebrauchsanweisung

Endress+Hauser

Endress+Hauser Liquistation CSF33 Bedienungsanleitung

Endress+Hauser

Endress+Hauser analytikjena Biometra TRobot II Handbuch

Endress+Hauser

Endress+Hauser ASP Station 2000 Bedienungsanleitung

Endress+Hauser

Endress+Hauser Analytik Jena Biometra TAdvanced 96 Bedienungsanleitung

Endress+Hauser

Endress+Hauser KIO1 Technische Daten

Endress+Hauser

Endress+Hauser Analytik Jena Automatic Boat Drive Bedienungsanleitung

Endress+Hauser

Endress+Hauser Analytik Jena PlasmaQuant MS Series Bedienungsanleitung

Endress+Hauser

Endress+Hauser Analytik Jena Biometra TRobot II 96 G Bedienungsanleitung

Endress+Hauser

Endress+Hauser analytikjena ZEEnit 700 Q Bedienungsanleitung

Endress+Hauser

Endress+Hauser Liquistation CSF48 Bedienungsanleitung

Endress+Hauser

Endress+Hauser Analytik Jena PlasmaQuant MS Bedienungsanleitung

Endress+Hauser

Endress+Hauser Liquistation CSF48 Handbuch

Endress+Hauser

Endress+Hauser Liquistation CSF48 Bedienungsanleitung

Endress+Hauser

Endress+Hauser Analytik Jena PlasmaQuant MS Series Bedienungsanleitung

Beliebte Laborausrüstung Handbücher anderer Marken

Qiagen

Qiagen DML 3000 Bedienungsanleitung

Queensgate

Queensgate NANOSCAN OP400 Programmierhandbuch

Conviron

Conviron GEN1000-GE Bedienungsanleitung

Parr Instrument

Parr Instrument 4560 Bedienungsanleitung

IKA

IKA IC control Bedienungsanleitung

Integra

Integra DOSE IT Bedienungsanleitung

Agilent Technologies

Agilent Technologies 5800 ICP-OES Bedienungsanleitung

NI PXI-5422 Handbuch

Collomix

Collomix Aqix Bedienungsanleitung

SPEX SamplePrep

SPEX SamplePrep 6875 Freezer/Mill Series Bedienungsanleitung

Ocean Insight

Ocean Insight FLAME-NIR+ Bedienungsanleitung

Parker

Parker ALIGN-MG-NA Bedienungsanleitung

BD 644787 Bedienungsanleitung

DENTAURUM

DENTAURUM Compact Megaplus Bedienungsanleitung

Biuged Laboratory Instruments

Biuged Laboratory Instruments BGD 626 Bedienungsanleitung

VWR

VWR SAS Super IAQ Bedienungsanleitung

illumina

illumina MiSeqDx Bedienungsanleitung

Yamato

Yamato BM510 Bedienungsanleitung