Endress+Hauser Analytikjena Biometra TRobot II Handbuch

Setup Instructions

Biometra TRobot II

Automated thermal cycler

Biometra GmbH (Analytik Jena GmbH)

Rudolf-Wissell-Str. 30

37079 Göttingen Germany

Phone + 49 551 50 68 6-0

Fax + 49 551 50 68 6-66

E-Mail info@analytik-jena.com

Biometra GmbH

Rudolf-Wissell-Str. 30

37079 Göttingen Germany

Phone + 49 551 50 68 6 -10, -14 or -16

Fax + 49 551 50 68 6-66

E-Mail service@analytik-jena.com

http://www.analytik-jena.com

These setup instructions are valid from software version ME 2.00 – RE 2.00

For a proper and safe use of this product follow the instructions.

Keep the setup instruction for future reference.

34-4000-002-23

B (08/2021)

Analytik Jena GmbH

Manufacturer

Service

General

information

Documentation number

Edition

Technical

documentation

produced by

Biometra TRobot II Contents

Contents

1Basic information ....................................................................................................... 5

1.1 Notes on this Setup Instruction ........................................................................................ 5

1.2 Intended use...................................................................................................................... 5

2Safety instructions...................................................................................................... 7

2.1 General notes .................................................................................................................... 7

2.2 Safety markings on Biometra TRobot II ........................................................................... 7

2.3 Technical condition ........................................................................................................... 8

2.4 Requirements for the operating personnel...................................................................... 8

2.5 Safety instructions: transport and installation ................................................................. 8

2.6 Safety instructions – during operation ............................................................................. 9

2.6.1 Summary of safety instructions ........................................................................................ 9

2.6.2 Safety instructions: protection against explosion and fire............................................... 9

2.6.3 Safety instructions: electrical equipment ......................................................................... 9

2.6.4 Handling of samples, auxiliary and operating materials ............................................... 10

2.7 Safety instructions – maintenance and repair ............................................................... 10

2.8 Behavior during emergencies ......................................................................................... 11

3Device installation and commissioning.................................................................... 12

3.1 Scope of delivery ............................................................................................................. 12

3.2 Unpacking and inspection............................................................................................... 12

3.3 Commissioning................................................................................................................ 12

3.3.1 Setting up the device....................................................................................................... 12

3.3.2 Connecting the cables ..................................................................................................... 14

3.4 Setting the operating voltage ......................................................................................... 15

3.5 Overview of the initial setup........................................................................................... 15

4Functional elements................................................................................................. 17

4.1 Biometra TRobot II PCR module front view ................................................................... 17

4.2 Biometra TRobot II PCR module rear view..................................................................... 18

4.3 Biometra TRobot II PCR controller front view................................................................ 19

4.4 Biometra TRobot II Controller rear view......................................................................... 19

5Operation.................................................................................................................. 20

Contents Biometra TRobot II

Biometra TRobot II Basic information

1Basic information

1.1 Notes on this Setup Instruction

As an excerpt from the detailed device instructions, these setup instructions contain the

first steps of installation and the safety instructions to be observed. For all further steps,

please apply the detailed operating instructions, which are supplied in electronic form

on a USB stick. Alternatively, you can also find the operating instructions on the Analytik

Jena homepage in the download area of the corresponding device.

Biometra TRobot II is intended for operation by qualified specialist personnel observing

the device operating manual and the operating manual for the Biometra TRobot II soft-

ware.

There are three different Biometra TRobot II models available. In the text below these

devices are collectively referred to as Biometra TRobot II. Differences are explained in

the corresponding chapters.

The operating manual contains information about the design and operation of Biometra

TRobot II and provides personnel familiar with PCR technology with the necessary

know-how for the safe handling of the device and its components. The operating man-

ual further includes notes on the maintenance and servicing of the device and potential

causes and remedies of any faults.

This instruction manual applies to all Biometra TRobot II devices from software version

ME 2.00 – RE 2.00.

Safety instructions are indicated by pictographs and signal words. The type and source

of the hazard are stated together with notes on preventing the hazard.

The operating manual uses the following symbols and signal words to indicate hazards

or instructions. Safety instructions are always placed before an action.

WARNING

Indicates a potentially hazardous situation which might cause fatal or very

serious inju-

ries (deformities).

CAUTION

Indicates a potentially hazardous situation which might cause light or minor injuries.

NOTE

Provides information on potential material or environmental damage.

1.2 Intended use

Biometra TRobot II is an automated end-point thermal cycler. Biometra TRobot II was

specially developed for integration into automation systems, e.g., in liquid handling

Conventions

Symbols and

signal words used

Basic information Biometra TRobot II

systems. For space-saving integration on robotic decks, the thermal cycler is divided into

a block module and a separate controller. The block module is placed on or next to the

robotic platform, the controller, which is connected by cable, usually under the platform.

Biometra TRobot II can be operated from an automation platform software. For this

purpose, Biometra TRobot II comes with a software library (dll file) that can be inte-

grated into the automation software.

Alternatively, Biometra TRobot II can be controlled via the Biometra TSuite computer

software included in the scope of delivery.

To facilitate automatic work flows, the thermal cycler lid opens and closes automatically,

allowing a robot arm to place the sample plate on the block and remove it again after

the program has been completed. To ensure the plate can be removed smoothly, even

after many heating and cooling cycles which may have caused the PCR plate to shrink

slightly or become distorted, Biometrat TRobot II features a patent-pending plate lifting

mechanism: When the lid is opened, the plate is slightly lifted up by four small pins that

move out of the block, thus detaching it from the block. This allows a robot arm to easily

remove the plate from the block.

A status LED at the front of the block module indicates the respective status of the ther-

mal cycler by means of color and luminous frequency.

Like a typical thermal cycler, the Biometra TRobot II automated thermal cycler is also

primarily designed to amplify nucleic acids by repeated heating and cooling cycles using

DNA polymerases in a PCR reaction. Other incubation of liquid samples in PCR tubes or

plates is also possible.

Deviations from the intended use described in this short instruction and in the operating

manual result in limitations of warranty and liability during a damage event. Damage to

wearing parts is not included in the warranty.

If the safety instructions given in this setup instruction and in the operating manual are

not observed when handling the device, this is considered a deviation from the intended

use.

The thermal cycler is developed for Research Use Only (RUO).

Biometra TRobot II Safety instructions

2Safety instructions

2.1 General notes

For your own safety and to ensure error-free and safe operation of Biometra TRobot II,

please read this chapter carefully before commissioning.

Besides the safety instructions in the operating manual and the local safety regulations

that apply to the operation of the device, the general applicable regulations regarding

accident prevention, occupational health and safety, and environmental protection have

to be observed and complied with.

References to potential hazards do not replace the work protection regulations which

must be observed.

2.2 Safety markings on Biometra TRobot II

Damaged or missing safety symbols can cause incorrect actions leading to personal in-

jury or material damage. Do not remove safety symbols! Replace damaged safety sym-

bols immediately!

The following safety symbols are attached to the inside of the heated lid and the rear of

the device:

Symbol Meaning Notes

Caution!

Hazard point

This symbol is located on the inside of the heated lid and on

the rear of the device.

Proceed with due care when handling samples and working

with Biometra TRobot II.

Caution!

Risk of burns

This symbol is located on the inside of the heated lid and on

the rear of the device.

There is a risk of burning at the heated lid, the thermal block,

the samples, and the rear of the device.

Ensure ventilation slots are

unobstructed

This sign is attached to the underside of the device.

Ensure that the ventilation slots on the underside and at the

rear of the device are unobstructed. Make sure that there are

no objects underneath the device (e.g., paper) that may be

sucked against the ventilation slot and thus interfere with

ventilation.

Biometra TRobot II must not be placed with the device feet

in recesses in higher base plates as it will cause ventilation

from the underside of the device to be restricted. Recesses

for the device feet as positioning aids on a platform may be

max. 1 mm deep.

Safety instructions Biometra TRobot II

2.3 Technical condition

Biometra TRobot II has been built and certified according to safety standard EN 61010-

1. Do not modify the device in any way. Any modification made to the device will lead to

a loss of warranty and the EN 61010-1 certificate and poses a potential risk.

The following has to be observed:

The operator must only operate the device in a sound and operationally safe condi-

tion. The technical condition must always comply with the legal requirements and

regulations.

Prior to every use the device must be checked for damage and sound condition.

Any changes in the device affecting its safety must be reported by the operating per-

sonnel to the operator without delay.

2.4 Requirements for the operating personnel

Biometra TRobot II must only be operated by qualified specialist personnel instructed in

the use of the device. The instruction also includes imparting the contents of the operat-

ing manual.

In addition to the safety at work instructions in the operating manual, the generally ap-

plicable safety and accident prevention regulations of the respective country of opera-

tion must be observed and adhered to. The operator must ascertain the latest version of

these regulations.

The operating manual must be accessible to the operating and service personnel at all

times.

2.5 Safety instructions: transport and installation

The following has to be observed:

Drain the sample block before transporting Biometra TRobot II. Make sure there are

no sample tubes in the block.

Only send Biometra TRobot II in its original packaging.

On receipt of Biometra TRobot II, please verify that the delivery is complete and

check for possible transport damage. In the event of a claim, please contact Analytik

Jena.

The Biometra TRobot II is designed for indoor use only and not for installation and

operation in a wet environment.

Biometra TRobot II Safety instructions

2.6 Safety instructions – during operation

2.6.1 Summary of safety instructions

Before each commissioning, the operator of Biometra TRobot II must make sure that the

condition of the device including the safety equipment is sound.

The following has to be observed:

Free access to the main power switch on the rear panel of device must always be en-

sured during operation.

The ventilation fittings at the rear of the device must be unobstructed and opera-

tional. Covered ventilation grilles or slots etc. may cause the device to break down or

may cause damage to it.

The use of oil between the samples and the sample block is not necessary to achieve

an improved heat exchange. However, if you still want to use oil, you should use

mineral oil. Do not use silicone oil.

Be careful to avoid crushing or pinching injuries when closing the device.

Biometra TRobot II may cause burns. The following has to be observed:

The thermal block, the samples, and the heated lid reach high temperatures. There is

a risk of burns during contact.

Ensure that the lid is securely closed before starting the program! Do not touch the

heated lid!

The rapid heating of the thermal block can cause liquids to boil explosively. Always

wear safety goggles during operation!

Do not touch hot sample tubes or plates and do not open them or boiling liquid may

escape!

Only use plates and tubes that are suitable for high temperatures (up to 100 °C), fit

well into the thermal block (no wobbling), and whose lids seal tightly!

Modifications and extensions to the device may only be carried out after consultation

with Analytik Jena. Unauthorized changes can limit the operating safety and lead to

restrictions in warranty and access to customer service.

2.6.2 Safety instructions: protection against explosion and fire

Biometra TRobot II must not be operated in an explosive environment. Biometra TRobot

II must not be operated with flammable, explosive, or volatile substances.

2.6.3 Safety instructions: electrical equipment

Work on the electrical components of Biometra TRobot II may only be performed by a

qualified electrical technician in accordance with the applicable electrical engineering

rules. Life-threatening electrical voltages may occur in the interior of Biometra TRobot

II!

The following has to be observed:

Any work on the interior of the device may only be carried out by Analytik Jena ser-

vice department and specially authorized technicians.

Safety instructions Biometra TRobot II

The electrical components must be checked regularly by a qualified electrician. Any

defects, such as loose connections, faulty, or damaged cables, must be repaired with-

out delay.

Before opening the device it must be switched off at the main power switch and the

power plug must be disconnected from the power outlet!

Biometra TRobot II must be switched off immediately using the main power switch

(on the rear panel of the controller) and the power plug disconnected from the

power supply if any faults occur in the electric components.

Do not operate the device near sources of strong electromagnetic radiation (e.g., a

non-shielded, deliberately operated high frequency source), as these may affect

proper operation of the device.

2.6.4 Handling of samples, auxiliary and operating materials

The operator is responsible for the selection of substances used in the process as well as

for their safe handling. This is particularly important for radioactive, pathogenic, infec-

tious, poisonous, corrosive, or otherwise hazardous substances. For details contact the

safety officer responsible for your location. When handling hazardous substances local

safety codes and guidelines must be observed. The following general notes do not re-

place the specific local regulations or the regulations in the safety data sheets of the

manufacturers for the auxiliary and operating materials.

The following has to be observed:

Protective goggles and rubber gloves have to be worn when handing reagents.

If only a few samples are to be treated, two (empty) tubes of the same height must

additionally be placed in the corner positions of the block. If the number of samples

in the block is too low, there is a risk of the tubes being damaged and sample liquid

leaking out.

For your own safety, please consider the potential risk of infection of the examined

biological material.

2.7 Safety instructions – maintenance and repair

Biometra TRobot II is usually repaired by the Biometra GmbH/Analytik Jena AG service

department or by authorized and trained specialist personnel. Unauthorized repairs can

damage the device. Therefore, the operator may generally only carry out the tasks listed

in the operating manual.

The following has to be observed:

The exterior of the device may only be cleaned with a damp, not dripping, cloth after

the device has been switched off.

Any maintenance on the device may usually only be carried out in the switched-off

condition (unless stated otherwise).

Use only original spare parts or consumables. Only these parts are tested and guar-

antee safe operation.

If the instrument is to be sent to the service department, please contact the Bi-

ometra/Analytik Jena service department beforehand. You will then receive an au-

thorization number. In addition, the device must be decontaminated before sending

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