Endress+Hauser Analytikjena Biometra TAdvanced Anleitung zur Verwendung

Short Manual Biometra TAdvanced

Analytik Jena GmbH

Konrad-Zuse-Strasse 1

07745 Jena ⋅ Germany

Phone +49 3641 77 70

[email protected]

www.analytik-jena.com

Short Manual Biometra TAdvanced

Version: A (07/2022)

34-3800-001-23

1/2

Safety instructions

Only use the quick reference guide in combination with the detailed operating manual.

WARNING!Risk of electric shock! Check whether the voltage selector switch (bottom) is

set to the correct mains voltage.

CAUTION!Risk of burns! Close the heated lid before starting the program. Use protective

equipment when handling hot samples.

NOTICE!Reduce the contact pressure for opening: Turn the wheel counterclockwise and

then press the button. After closing the lid, apply the contact pressure via the lid wheel.

Design

¡Wheel for exerting the contact pressure(1)

¡Lid sealing button(2)

¡Touch screen display with control software(3)

¡USB port(4)

After the initial self test, the software first opens the login screen and then the Home screen.

Option Icon Description

Quick Start Block Display of the current block status. If the block is free:

Tap to open the quick-start function

DE/EN/CN /¡Change login screen language

¡Set the software language in the user management

Programs ¡Create or edit PCR programs

¡Select a program and start the PCR run

Protocol Wizard ¡Automatically create a PCR program

¡Calculate the annealing temperature and holding

time for the elongation step

Incubate Incubate the samples at constant temperature

Tools ¡Customize the software settings

¡Set up the user management

¡View documentation, self test

¡Create Service info file, carry out a backup

Free

Quick Start

Programs Incubate Tools

Logout

28.0°C

Block 1

User: John Doe

dd.mm.yyyy hh:ss

Protocol Wizard

Free

Quick Start

Programs Incubate Tools

Logout

28.0°C

Block 1

User: John Doe

dd.mm.yyyy hh:ss

Protocol Wizard

Quick Start

28.0°C

Free

Block 2

Home screen (mono block) Home screen (twin block)

Creating or editing PCR programs

}Tap Programs on the Home screen.

}Select a program on the page Program overview and edit it. Or: Tap New from tem-

plate. Select a program template and tap Open template to open it.

}Use or to select the graphical or the spreadsheet programming mode.

Name: Lid: Preheat Lid: On

Home Edit Insert Delete Done Back

Step

Go To

Cycles

∆T

∆t

∆R

30x

1 2 3 4 5 6Step Step Step Step Step

Go To

Temperature [°C]

Three-step

dd.mm.yyyy hh:mm

Program Runtime: 1h 15m 10s

User: John Doe

Name: Lid: Preheat Lid: On

Home Edit Insert Delete Done Back

Loops Step Block Temp. (°C)

Hold Time

(h:mm:ss) Go To Cycles ∆T(°C) ∆t(s) ∆R(°C/s)

Three-step

dd.mm.yyyy hh:mm

Program Runtime: 1h 15m 10s

User: John Doe

Graphical programming mode Spreadsheet programming mode

}Enter the program name under Name:. Define the temperature for the heated lid under

Lid:. Select On to activate the preheating function of the heated lid.

}Use Insert and Delete to add or delete steps. Maximum number of steps: 30

}Select a program step for editing. Change individual parameters in graphical/spreadsheet

view. Or: Tap Edit and change all parameters.

}Define the block temperature (3to99°C) and the holding time (max. ≤9h59min59s).

Use ∞ to program a pause.

}If required, define optional program parameters.

}Tap Done. Select a user directory under Copy/save programs.

}Save the program. Or: Enter a new name under Save as.

Short Manual Biometra TAdvanced

Analytik Jena GmbH

Konrad-Zuse-Strasse 1

07745 Jena ⋅ Germany

Phone +49 3641 77 70

[email protected]

www.analytik-jena.com

Short Manual Biometra TAdvanced

Version: A (07/2022)

34-3800-001-23

2/2

Setting optional program parameters

}Open a program or a program template for editing. Select a step.

}Program loops: Define to which step the program shall return under GoTo. Define the

number of reiterations under Cycles. Maximum: 999

}Define a temperature increment (±0.1to20°C) under ΔT by which the temperature shall

increase/decrease per cycle.

}Define a time increment (1to240s) under Δt by which the holding time shall increase

per cycle.

}Set the heating and cooling rate precisely in steps of 0.1°C/s under ΔR.

}In order to optimize the annealing temperature, define the temperature gradient in de-

grees by which the block temperature shall increase or decrease from column to column

(mono block) or from row to row (twin block), see below.

}If prompted, confirm your entries. Tap Done. Tap Save to save the program.

Program the temperature gradient (gradient-enabled models)

}Open a program or a program template for editing. Select the annealing step.

}Tap the field for the block temperature. Tap Degrees on the keypad. Or: In the graphical/

spreadsheet view, first tap Edit and then Degrees.

}Tap Lin./Std. to choose between the Linear Gradient Tool and the standard gradient pro-

gramming feature.

Annealing Temp.:

Column

Grad. incr.:

Lin.

Std.

Col.

Step Gradient3

Annealing Temp.:

Column

Grad. incr.:

Lin.

Std.

Col.

Step Gradient3

Mono block: The gradient is horizontal.

(see operating manual)

Twin block: The gradient is vertical.

“Column” (line) 1 starts at the bottom left.

}Linear Gradient Tool: Enter the annealing temperature under Annealing Temp.:. This

temperature is applied to the column which is indicated under Annealing Temp.:. Enter a

temperature increment under Grad. incr.:.

}Standard gradient programming: Enter the temperature for the first and the last column.

}Confirm your entries. Tap Done. Tap Save to save the program.

Copying and deleting programs

Option Icon Description

Copy all Copy all programs of the selected user

Copy Copy the selected program

Delete all Delete all programs of the selected user

Delete Delete the selected program

Starting, stopping and pausing programs

}Use the quick-start function on the login screen/Home screen, to start the PCR run.

}Or: Tap Programs on the Home screen. Select the user directory and the program on the

page Program overview.

User: Admin dd.mm.yyyy hh:mm

Home EditDelete

Program overview

New from

template

Copy Start

User

users3

USB

Admin

John Doe

Programs

in directory3 Preview

Step

GoTo

Program information

Name

Block Type:

Lid:

Program Runtime:

Lin-grad-dag

Gradient 96

99 °C Preheat Lid: On

01h 15m 22s

30x 2

}Use the arrow keys to navigate through the list, if the number of users/programs is

greater than what can be displayed.

Or: Use to display all users/programs.

}Use the preview function to validate the program and tap Edit to adjust it, if required.

}Insert samples into the thermal block. Use replicates of a sample to optimize the anneal-

ing temperature. Close the lid. Establish the contact pressure: Turn the wheel clockwise

until you hear a clicking sound.

}Tap Start to start the program.

}Select one or both blocks in the twin-block prompt Please select block to start:. Confirm

your selection.

}Tap Pause to stop the currently executed program. Tap Continue to resume the program.

Tap Skip to skip a step. Tap Stop program to abort a PCR run.

}Tap Choose block stop program to select one or several blocks.

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