Labnet Spectrafuge 24D Bedienungsanleitung

Instruction Manual

Labnet

Spectrafuge 24D Microcentrifuge

Labnet International

PO Box 841

Woodbridge, NJ 07095

Phone: 732 417-0700

Fax: 732 417-1750

email: [email protected]

Reproduction without prior written permission is prohibited.

Information contained herein is subject to change without notice.

Safety Precautions

NEVER use the centrifuge in any manner not specified in these instruc-

tions.

NEVER operate the centrifuge without a rotor properly attached to the

shaft.

NEVER fill tubes while they are in the rotor. Liquid spillage may harm

unit.

NEVER put hands in the rotor area unless the rotor is completely stopped.

NEVER move the centrifuge while the rotor is spinning.

NEVER use solvents or flammables near this or other electrical equip-

ment.

NEVER centrifuge flammable, explosive or corrosive materials

NEVER centrifuge hazardous materials outside of a hood or proper

containment facility

ALWAYS load the rotor symmetrically. Each tube should be counterbal-

anced by another tube of the same type and weight

ALWAYS locate the centrifuge within easy access to an electrical outlet.

ALWAYS use only microcentrifuge tubes made from plastic and designed

to withstand centrifugal forces of at least 16,500 xg.

Table of Contents

1. General Information 1

Description

Safety precautions

Technical data

Accessories supplied with unit

Warranty

2. Installation 2

Unpacking the centrifuge

Required space

Installing the centrifuge

3. Installing the Rotor 3

Rotors and accessories

Rotor maintenance

Removing and installing the angle rotor

Loading the rotor

Overloading rotors

4. Operation 6

Attaching rotor lid

Closing the lid

Lid release

Lid lock

Speed selection (RPM / g force)

Selection of operating time, momentary operation, Start/Stop

5. Service and Maintenance 8

Service

Cleaning

Disinfection

Replacing fuses

6. Troubleshooting 9

7. Where to Call 11

8. Determination of g-values 12

1. General Information

This manual provides important safety information for the Spectrafuge 24D

laboratory microcentrifuge. It should be kept near the centrifuge for quick

and easy reference.

1.1 Description

The Spectrafuge 24D is a small benchtop centrifuge designed for

separation of various research samples. The motor is brushless and

requires no routine maintenance. The Spectrafuge 24D is supplied

with a 24 x 1.5/2.0ml rotor for micro samples. Adapters are avail-

able for tubes smaller than 1.5ml. The Spectrafuge 24D reaches

speeds of up to 13,300rpm/16,300 x g.

1.2 Safety precautions

Note: All users of the centrifuge must read the Safety Pre-

cautions section of this manual before attempting to operate

the unit!

If this equipment is used in a manner not specified by the

manufacturer, the protection provided by the equipment may

be impaired.

Do not operate the centrifuge if any of the following conditions exist:

-The centrifuge has not been installed properly

-The centrifuge is partially dismantled

-Service has been attempted by unauthorized or unqualified person-

nel

-The rotor has not been installed securely on the motor shaft

-Rotors and accessories not belonging to the standard range are

being used without permission being obtained from the manufac-

turer to use such rotors and/or accessories in the centrifuge

Exception: Microcentrifuge tubes made of plastic, normally avail-

able in the laboratory.

-The centrifuge is located in an explosive atmosphere

-Materials to be centrifuged are combustible and/or explosive

-Materials to be centrifuged are chemically reactive

-The rotor load is not properly balanced

Notes

1.3 Technical data

Dimensions

Width 9.25 inches

Depth 11.5 inches

Height 8.5 inches

Maximum speed 13,300rpm

Maximum RCF 16,300 x g

Maximum volume 24 x 1.5/2.0ml

Admissible sample density 1.2kg/dm3

Electrical/fuse rating 120V~, 50-60Hz, 1.3A, 2.5 AT

230V~, 50-60Hz, 0.7A, 1.25 AT

Operating temp./humidity +4ºC to 35ºC - 80%RH

1.4 Accessories supplied with centrifuge

Each unit is supplied with 1 instruction manual, 1 warranty card

1 power cord, a standard rotor and a rotor removal tool.

1.5 Warranty

This centrifuge has been subjected to thorough testing and quality

control. In the unlikely event of a manufacturing fault, our one

year warranty (from the date of delivery) covers the centrifuge

and the rotor. This warranty becomes invalid in the case of

incorrect operation, use of nonstandard spare parts or accesso-

ries and unauthorized modification of the rotor or centrifuge.

Labnet reserves the right to make technical modifications.

Please see the complete limited warranty statement supplied

separately.

2. Installation

2.1 Unpacking the centrifuge

Before unpacking the centrifuge, inspect the outside of the carton

for any shipping damage.

The centrifuge is delivered in a carton with protective foam

cushioning. Remove the centrifuge from the carton. Retain the

carton and cushioning until it has been established that the

centrifuge is working properly.

Inspect the centrifuge for any visible signs of shipping damage.

Shipping damage is the responsibility of the transportation carrier.

Any claims for damage must be filed within 48 hours with the

carrier that delivered the centrifuge.

The accessories supplied with the centrifuge should be kept with

the instruction manual near the centrifuges place of installation.

2.2 Required space

The centrifuge should be installed on a rigid, even surface such

as a stable laboratory bench, countertop, etc. To guarantee

sufficient ventilation, ensure that the centrifuge has at least 15cm

(6 inches) of free space on all sides, including the rear.

The centrifuge should not be located in areas subject to excessive

heat such as in direct sunlight or near radiators or the exhaust of

a compressor, as a buildup of heat may occur within the chamber.

2.3 Installation

Before operating the centrifuge, check that the power source

(electrical outlet on the wall) corresponds to that on the

manufacturers rating label, then connect the power cord to the

centrifuge and the power source.

3. Installation of rotors and rotor maintenance

3.1 Rotors and accessories

The following accessories are included or available for the

Spectrafuge:

Angle rotor for 24 x 1.5ml tubes (Included)

Tube measurement 1.5ml / 2.0ml (10 x 40mm)

Max. speed 13,300rpm

Centrifuging radius 8.23cm

Max. RCF (g-value) 16,276 x g

Rotor Wrench (Included)

Order no. C0240-RT

Rotor Lid (Included)

Order no. C0240-RC

Rotor Securing Screw (Included)

Order no. C0240-RSS

312

8. Determination of g-values

The centrifuging radius of the 1.5/2.0ml rotor is 8.23cm. The

Spectrafuge 24D has an automatic g-force conversion program, so g-

values are automatically calculated and can be displayed on the

control panel, for this centrifuging radius. If adapters or smaller tubes

are used, the centrifuging radius changes as does the g force. The

chart on the next page can be used to manually determine g-values

for any centrifuging radius.

Strip Spin Adapter

Order no. C0240-SS

Tubes accepted 0.2ml tubes or 8 x 0.2ml strips

Max Speed 13,300 rpm

Range Centrifuge radius 4.32cm to 5.33cm

Range RCF (g value) 8,549 x g to 10,539 x g

Adapter for 0.5ml tubes (pk./6)

Order no. C-1205

Tube measurement 8 x 30mm

Max. speed 13,300rpm

Centrifuging radius 7.53cm

RCF (g-value) 14,892 x g

Adapter for 0.4ml tubes (pk./6)

Order no. C-1206

Tube measurement 6 x 47mm

Max. speed 13,300rpm

Centrifuging radius 8.23cm

RCF (g-value) 16,276 x g

Adapter for 0.2ml tubes (pk./6)

Order no. C-1222

Tube measurement 6 x 21mm

Max. speed 13,300rpm

Centrifuging radius 7.03cm

RCF (g-value) 13,903 x g

3.2 Rotor maintenance

The rotor should be cleaned thoroughly after each use. Thor-

ough cleaning must be performed when spinning samples

containing phenol or phenol chloroform. Periodically inspect

the rotor for dents, dings, scratches, discoloration and cracks. If

any damage to the rotor is found, discontinue use of the rotor

immediately and replace.

3.3 Removing and installing the angle rotor

The Spectrafuge 24D comes complete with a standard 24 place

rotor installed. To remove the rotor for cleaning, remove the

rotor securing screw from the motor shaft by turning the screw

counterclockwise, using the rotor wrench. Lift the rotor directly

upward in a straight vertical motion.

Possible reason: Sample liquid in tubes not balanced

Solution: Make sure the same volume of liquid is

in each tube

Possible reason: Defective or improperly adjusted

balance sensor

Solution: Call service

Er LII

Id: Lid not closed

Possible reason: Lid not closed completely

Solution: Close lid

Possible reason: Lid lock or sensor defective

Solution: Call service

7. Where to call

Should you have any questions about the Spectrafuge or its accesso-

ries, please call Labnets Customer Service Department at 732 417-

0700. Customer Service is staffed from 8:30am to 5:30pm, EST,

Monday through Friday. Our 24 hour fax number is 732 417-1750.

Inquiries may also be sent via our electronic mailbox at

[email protected].

Should your Spectrafuge require service, please call Labnets Techni-

cal Services Department at 732 417-0700. Our Service Department

is staffed from 8:30am to 5:00pm, EST, Monday through Friday. Our

24 hour fax number is 732 417-1750. Electronic mail may be sent to

[email protected].

Please have the units serial number (located on the back panel of the

instrument) available when calling. Should an item require return to

Labnet for service, a repair authorization (RA) number must first be

received from Labnet and a decontamination form completed by the

user. Items sent without an RA number or decontamination form will

not be accepted.

Figure 1. Loading the rotor to achieve balance

510

6. Troubleshooting Guide

Please refer to this guide before calling for service.

Centrifuge will not start

Possible reason: No power supply

Solution: Check that power is being supplied to

the outlet

Check that the power cord is plugged

into both the wall outlet and the back of

the centrifuge

Check that power cord is not damaged

Possible reason: Blown fuse

Solution: Check fuse and replace if necessary

Lid lock will not release

Possible reason: Defective lid lock

Solution: Open manually and have unit serviced

Possible reason: No power from PC board

Solution: Call for service

Possible reason: Lid lock is jammed

Solution: Call for service

Possible reason: Centrifuge is not receiving power

Solution: See Centrifuge will not start

Centrifuge cannot be started, although power is on

Possible reason: Lid not closed correctly

Solution Close lid correctly

Possible reason: No speed or time has been selected

Solution: Set speed and/or time

Error Messages (press timer or speed knob to clear error

messages)

Er BAL: Indicates imbalance

Possible reason: Tubes not inserted symetically in rotor

holes

Solution: Load tubes symetically

(see Section 3.4 on Loading the rotor)

To replace rotor, first make sure the motor shaft and rotor

mounting hole are clean. Place the rotor on the motor shaft.

Reinstall the rotor securing screw on the motor shaft by turning it

clockwise. Hold the rotor with one hand and tighten the rotor

securing screw, using the rotor wrench.

3.4 Loading the rotor

Tubes to be loaded should be filled equally by eye. The differ-

ence in the weight between the tubes should not exceed 0.1

gram. Tubes should always be loaded so that there is equal

spacing between all tubes. One or two additional loaded tubes

may need to be added to achieve this. Refer to Figure 1. to see

one typical balancing scheme.

3.5 Overloading rotors

The maximum load of the rotor and the maximum speed have

been established by the manufacturer. Do not attempt to exceed

these values. The maximum speed of the rotor has been estab-

lished for liquids having a homogeneous density of 1.2g/ml or

less. In order to centrifuge liquids with a higher density it is

necessary to reduce the speed. Failure to reduce the speed

may result in damage to the rotor and centrifuge. The

revised maximum speed can be calculated with the following

formula:

Reduced speed (nred) = x max speed (nmax)

Example:

Where the density of the liquid is 1.7, the new maximum speed

would be calculated as follows:

nred = x 13,300 = 9,882 rpm

If in doubt concerning maximum speeds, please contact the

manufacturer for assistance.

1.2

higher density value

1.2

1.7

5. Service and Maintenance

5.1 Centrifuge service

The brushless motor in the Spectrafuge 24D requires no routine

maintenance. Any required service should be performed by

authorized, qualified personnel only. Repairs performed by

unauthorized personnel may void the warranty.

5.2 Cleaning the centrifuge

Always keep the centrifuge housing, rotor chamber, rotor and

rotor accessories clean. All parts should be wiped down periodi-

cally with a soft cloth. For more thorough cleaning, use a neutral

cleaning agent (pH between 6 and 8) applied with a soft cloth.

Excessive amounts of liquid should be avoided. Liquid should not

come into contact with the motor. After cleaning, ensure that all

parts are dried thoroughly by hand or in a warm air cabinet

(maximum temperature 50ºC)

5.3 Cleaning the rotor

The rotor should be cleaned after each use. When spinning

samples containing phenol or phenol chloroform, the rotor should

be cleaned immediately after use.

5.4 Disinfection

Should a spill of infectious materials occur within the rotor or

chamber, the unit should be disinfected. This should be per-

formed by qualified personnel with proper protective equipment.

5.5 Replacing fuses

Check the fuse when it is recommended in the Troubleshooting

Guide located in this manual. The fuse holder is located in the

power inlet on the rear of the unit. Disconnect the power cord

from the power inlet. Open the fuse holder drawer by inserting a

small screwdriver under the tab and prying it open. Remove the

innermost (operative) fuse from its retaining tabs and replace the

fuse if necessary. A spare fuse is located in the outermost

chamber of the fuse drawer. Replace only with a fuse of exactly

the same value as the original. (Fuse type may be found in the

Technical data section of this manual.)

4. Operation

ATTENTION: Never attempt to operate the centrifuge with

rotors or adapters that show signs of corrosion or mechanical

damage. Never centrifuge strongly corrosive materials that

may damage the rotors, accessories, or bowl of the unit.

4.1 Attaching rotor lid

After the rotor has been properly secured and loaded, attach the

rotor lid to the rotor. Always use the rotor lid for safety and to

allow the rotor to reach proper speed. Make sure that the rotor

lid snaps securely into place, by pressing down on center catch.

4.2 Closing the lid

Close the centrifuge lid. The Spectrafuge 24D has a lid lock that

activates only when a run is started.

4.3 Lid release

The lid will remain locked during a centrifuge run. Once the run

has been completed and the rotor has come to a stop, a beep will

indicate the end of a run, and the lid will unlock automatically.

WARNING: Do not attempt to open the lid of any centri-

fuge until the rotor has come to a complete stop.

In the event of a power failure or malfunction, it may be neces-

sary to open the lid manually.

1. Disconnect the power cord from the wall socket.

2. Remove the plastic plug, located on the left side of the unit.

3. Pull the wire (attached to the plug) to open the lid lock

manually.

4.4 Lid lock

The centrifuge can be started only with the lid securely closed.

When a run is started, the lid lock automatically activates. Do

not attempt to open the lid during a centrifuge run. At the end of

the run, the lid will automatically unlock.

Never attempt to override the lid lock mechanism. Doing so is

dangerous and could damage the centrifuge.

4.5 Speed selection (see Figure 2.)

The speed (rpm or g-force) can be selected from 500 to 13,300

rpm or from 100 to 16,300 x g with the knob on the right. Select

g-force or rpm to be displayed by pressing the knob. Turn the

knob to increase or decrease the value.

4.6 Selection of operating time, momentary operation,

Start/Stop

Operating time can be selected from 0.5 min to 30 min by turning

the timer knob on the left. After 30 minutes, the display shows --

which indicates continuous run. In this mode, the centriufge will

run until manually stopped. To start a run, press the timer

knob.

When the preselected time expires, the centrifuge will stop auto-

matically. To stop the centrifuge prior to the expiration of set

time, press the timer knob.

The centrifuge may be operated for a short run by pressing and

holding the timer knob. The centrifuge will continue to run as long

as the timer knob is depressed and the time, in seconds will count

up on the time display.

Pressing either knob will clear error messages.

Figure 2. Spectrafuge control panel layout

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