Leica DM4000M Bedienungsanleitung

Leica DM4000 B

Leica DM4000 M

Leica DM4500 P

Leica DM5000 B

Operating Manual

Bedienungsanleitung

Published September 2007 by:

Herausgegeben September 2007 von:

Leica Microsystems CMS GmbH

Ernst-Leitz-Straße

D-35578 Wetzlar (Germany)

Responsible for contents:

Verantwortlich für den Inhalt:

Dr. Jasna Roeth, Stefan Motyka

(Marketing CM, Compound Microscopy, Product Management)

(Marketing CM, Compound Microscopy, Produktmanagement)

Holger Grasse

(Safety Officer according to MPG §30)

(Sicherheitsbeauftragter nach MPG §30)

In case of questions, please contact the hotline:

Bei Fragen wenden Sie sich bitte an die Hotline: Phone +49(0)64 41-29 22 86

Fax +49(0)64 41-29 22 55

E-Mail: [email protected]

Leica DM4000 B

Leica DM4000 M

Leica DM4500 P

Leica DM5000 B

Operating Manual

Copyrights

All rights to this documentation are held by

Leica Microsystems CMS GmbH. Reproduction

of text or illustrations (in whole or in part) by

print, photocopy, microfilm or other method

(including electronic systems) is not allowed

without express written permission from Leica

Microsystems CMS GmbH.

The term "Windows" may appear in the following

text without further identification. It is, however,

a registered trademark of Microsoft Corpora-

tion. The names of companies and products

used herein may be trademarks of their respec-

tive owners.

The instructions contained in the following doc-

umentation reflect state-of-the-art technology

and knowledge standards. We have compiled

the texts and illustrations as accurately as pos-

sible. Nevertheless, no liability of any kind may

be assumed for the accuracy of this manual’s

contents. Still, we are always grateful for com-

ments and suggestions regarding potential mis-

takes within this documentation.

The information in this manual is subject to mod-

ification at any time and without notification.

Contents

7. Startup ......................................................... 35

7.1 Functional principle .................................. 35

7.2 Switching on the unit ................................ 38

7.3 The display

(DM4000 B/4500 B/4000 M/4500 P)......... 39

7.4 The function keys ...................................... 40

7.5 Köhler illumination .................................... 41

7.5.1 Transmitted light ............................. 41

7.5.2 Incident light.................................... 42

7.6. Checking the phase contrast rings ........ 44

7.7 Setting the motorized polarizer

(DM4500 P/DM5000 B) .............................. 45

7.8 Adjusting the light sources...................... 45

8. Operation .................................................... 51

8.1 Switching on the unit ................................ 51

8.2 Stages and object displacement............ 51

8.3 Focusing ...................................................... 53

8.4 Tubes....................................................... 53

8.5 Eyepieces .................................................... 55

8.6 Objectives ................................................... 55

8.7 Magnification changer ............................. 58

8.8 HC P 1x/1.6x tube optics........................... 58

8.9 Light sources .............................................. 59

8.10 Aperture diaphragm and

field diaphragm .......................................... 59

Contents

1. Important notes about this manual ....... 7

2. Intended purpose of the microscope .... 8

3. Safety notes................................................ 9

3.1 General safety notes................................. 9

3.2 Electrical safety ......................................... 10

3.3 Disposal ....................................................... 11

4. Overview of the instrument .................... 12

5. Unpacking the microscope..................... 17

6. Assembling the microscope ................... 19

6.1 Specimen stage ......................................... 19

6.2 Condenser ................................................... 21

6.3 Tube and eyepieces .................................. 22

6.4 Objectives ................................................... 23

6.5 Light sources for the transmitted

light axis ...................................................... 23

6.6 Light sources for the incident

light axis ...................................................... 25

6.7 Equipping the incident light

turret disk .................................................... 30

6.8 Polarizer and analyzer .............................. 31

6.9 DIC prisms ................................................... 32

6.10 Optional accessories ................................ 33

6.11 Connection to the power supply ............ 34

6.12 Connecting to the CTR5000

electronics box .......................................... 34

Contents

9. Contrast methods for

Leica DM4000 B/DM4500 B/

DM4500 P/DM5000 B ................................ 60

9.1 Transmitted light ........................................ 60

9.1.1 Bright field ......................................... 60

9.1.2 Phase contrast.................................. 60

9.1.3 Dark field............................................ 61

9.1.4 Polarization........................................ 61

9.1.4.1 Manual method ............................. 61

9.1.4.2 DM4500 P - examinations

in polarized transmitted light...... 62

9.1.4.3 Motorized method ........................ 68

9.1.4.4 Combined methods....................... 68

9.1.5 Differential interference

contrast ............................................ 68

9.1.5.1 DM4500 B/DM4500 P .................... 68

9.1.5.2 DM5000 B........................................ 69

9.2 Fluorescence.............................................. 70

10. Contrast methods for

Leica DM4000 M ........................................ 71

10.1 Incident light .............................................. 71

10.1.1 Bright field ....................................... 71

10.1.2 Dark field.......................................... 71

10.1.3 Polarization...................................... 72

10.1.4 Interference contrast .................... 73

10.2 Transmitted light ........................................ 73

10.2.1 Bright field ....................................... 73

10.2.2 Polarization...................................... 73

11. Troubleshooting......................................... 74

12. Care of the microscope ........................... 77

12.1 Dust cover................................................... 77

12.2 Cleaning....................................................... 77

12.3 Handling acids and bases ....................... 78

13. Essential wear and spare parts ............. 79

14. Abbreviations and pictograms ............... 80

15. Index ............................................................ 81

16. EU Declaration of Conformity................. 82

1. Important notes about this manual

(1.2)

→p.20

Numbers in parentheses, such as "(1.2)", corre-

spond to illustrations (in the example, Figure 1,

Item 2).

Numbers with pointer arrows (for example →

p.20), point to a certain page of this manual.

Caution!

Special safety instructions within this manu-

al are indicated with the triangle symbol

shown here, and have a gray background.

Caution! The microscope and accessories can

be damaged when operated incorrectly.

Explanatory note.

Instructions on disposing of the microscope,

accessory components and consumables.

Item not contained in all configurations.

Text symbols, pictograms and their meanings:

Caution!

This operating manual is an essential com-

ponent of the microscope, and must be read

carefully before the microscope is assem-

bled and put into operation.

1. Important notes about this manual

This operating manual contains important in-

structions and information for the operational

safety and maintenance of the microscope and

accessories. It must therefore be kept safely for

future reference.

2. Intended purpose of the microscope

The DM4000 – DM5000 microscopes to which

these operating instructions belong, and which

have the identifying letter B, are intended for

biological routine and research applications.

This includes examining specimens taken from

the human body for the purpose of gaining infor-

mation about physiological or pathological con-

ditions or inborn anomalies, or testing for safety

and compatibility for potential recipients, or for

monitoring therapeutic measures.

The microscopes that have the identifying let-

ters M or P are intended for materials science,

geological or mineralogical examinations.

The above-named microscopes comply with the

Council Directive 98/79/EEC concerning in vitro

diagnostics. They also conform to the Council

Directives 73/23/EEC concerning electrical ap-

paratus and 89/336/EEC concerning electromag-

netic compatibility for use in an industrial envi-

ronment.

Caution!

The manufacturer assumes no liability for

damage caused by, or any risks arising from

using the microscopes for other purposes

than those for which they are intended or

not using them within the specifications of

Leica Microsystems CMS GmbH.

In such cases the declaration of conformity

shall cease to be valid.

Caution!

These (IVD) instruments are not intended for

use in the patient environment defined by

DIN VDE 0100-710. Nor are they designed to

be combined with medical instruments in

accordance with EN 60601-1. If a

microscope is electrically connected to a

medical instrument in accordance with

EN 60601-1, the requirements defined in

EN 60601-1-1 shall apply.

3. Safety notes

3.1 General safety notes

This safety class 1 device was built and tested

in accordance with the safety regulations for

electrical measuring, control, regulating and

laboratory devices in accordance with

EN 61010-2-101:2002

EN 61010-1:2001

IEC 1010-1:2001

Caution!

In order to maintain this condition and to en-

sure safe operation, the user must follow the

instructions and warnings contained in this

operating manual.

Caution!

The devices and accessories described in

this operating manual have been tested for

safety and potential hazards.

The responsible Leica affiliate or the main

plant in Wetzlar, Germany, must be consult-

ed whenever the device is altered, modified

or used in conjunction with non-Leica

components that are outside of the scope of

this manual.

Unauthorized alterations to the device or

noncompliant use shall void all rights to any

warranty claims and void product liability!

3. Safety notes

Caution!

The power plug may only be plugged into an

outlet equipped with a grounding contact.

Do not interfere with the grounding func-

tion by using an extension cord without a

ground wire. Any interruption of the ground

wire inside or outside of the device, or re-

lease of the ground wire connection, can

cause the device to become hazardous.

Intentional ground interruption is not permit-

ted!

Caution!

Through connection to the grounding con-

nection, ancillary equipment with its own

and/or extra power supply may be brought

to the same ground wire potential. For con-

nections without a ground connector, Leica

Service must be consulted.

90-250 V~

50-60 Hz

max. 155VA

2xT2A (IEC 127)

10-36°C

max. 80% to 30°C

90-250 V~

50-60 Hz

max. 290VA

T6.3 A

(IEC 60127-2/3)

15-35°C

max. 80% to 30°C

3.2 Electrical safety

General specifications

Leica CTR5000 electronics box (for DM5000 B)

For indoor use only.

Supply voltage:

Frequency:

Power input:

Fuses:

Ambient temperature:

Relative humidity:

Overvoltage category:

Pollution degree:

Microscope

For indoor use only.

Supply voltage:

Frequency:

Power input:

DM4000

DM4500

DM5000

Fuses:

DM4000

DM4500

DM5000

Ambient temperature:

Relative humidity:

Overvoltage category:

Pollution degree:

ebq 100 supply unit*

For indoor use only.

Supply voltage:

Frequency:

Power input:

Fuses:

Ambient temperature:

Relative humidity:

Overvoltage category:

Pollution degree:

(see enclosed manual)

90-250 V~

50-60 Hz

max. 180 VA

max. 290VA

T6.3 A (IEC 60127-2/3)

See CTR5000

15-35°C

max. 80% to 30°C

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