Leica DMI4000B Bedienungsanleitung

Leica DMI3000B,

DMI4000B, DMI6000B

Instructions · Bedienungsanleitung · Mode d’emploi

Published August 2010 by:

Herausgegeben August 2010 von:

Edité en août 2010 par :

Leica Microsystems CMS GmbH

Ernst-Leitz-Straße 17-37

D-35578 Wetzlar (Germany)

Responsible for contents:

Verantwortlich für den Inhalt:

Responsable du contenu rédactionnel :

Bernard Kleine

(Marketing CMS, Life Science Research Microscopy, Product

Management)

(Marketing CMS, Life Science Research Microscopy, Produkt-

management)

(Marketing CMS, Life Science Research Microscopy,

chef de produit)

Dietmar Gnass

(R&D Manager)

In case of questions, please contact the hotline:

Bei Fragen wenden Sie sich bitte an die Hotline:

Pour toute question, contacter notre service d’assistance

téléphonique : Phone: +49(0) 64 41 - 29 42 53

Fax: +49 (0) 64 41 - 29 22 55

E-Mail: [email protected]

Leica DMI3000B,

DMI4000B, DMI6000B

Instructions

Copyrights

All rights to this documentation are held by Lei-

ca Microsystems CMS GmbH. Reproduction of

text or illustrations (in whole or in part) by print,

photocopy, microﬁlm or other method (including

electronic systems) is not allowed without ex-

press written permission from Leica Microsys-

tems CMS GmbH.

The term „Windows“ may appear in the following

text without further identiﬁcation. It is, however,

a registered trademark of Microsoft Corpora-

tion. The names of companies and products used

herein may be trademarks of their respective

owners.

The instructions contained in the following docu-

mentation reﬂect state-of-the-art technology

standards. We have compiled the texts and illus-

trations as accurately as possible. Nevertheless,

no liability of any kind may be assumed for the

accuracy of this manual’s contents. Still, we are

always grateful for comments and suggestions

regarding potential mistakes within this docu-

mentation.

The information in this manual is subject to modi-

ﬁcation at any time and without notiﬁcation.

Contents

6.10 Installation and Replacement of the

transmitted Light Lamps:

107 or 107/2 Lamp Housing........................ 45

6.11 Installing the Lamp Housing Mount

and Mirror Housing)................................... 46

6.12 Installation and Replacement of

Incident Light Lamps.................................. 48

6.13 Equipping the Incident Light

Turret Disk.................................................... 52

6.14 Inserting the Front Module Slider............ 55

6.15 Installation of the Polarizer

and Analyzer................................................ 55

6.16 Optional Accessories................................. 57

6.17 Connection to the Electronics Box.......... 58

6.18 Connection to the Computer..................... 59

6.19 Connection to the Power Supply ............. 59

7. Start-up ........................................................ 60

7.1 Functional Principle .................................. 60

7.2 Switching on the Microscope.................. 64

7.3 The LeicaDisplay ........................................ 65

7.4 The Function Buttons on the Stand......... 66

7.5 The SmartMove

Remote Control Module ............................ 69

7.6 Illumination.................................................. 69

7.6.1 Transmitted light .............................. 69

7.6.2 Incident Light - Fluorescence........ 73

7.7 Checking Phase Contrast Rings............... 74

7.8 Checking modulation contrast

slit diaphragms............................................ 77

7.9 Setting the Motorized Polarizer ............... 77

7.10 Adjusting the Light Sources ..................... 78

Contents

1. Important Notes about this Manual........ 7

2. Intended Purpose of the Microscope..... 8

3. Safety Notes................................................ 9

3.1 General Safety Notes ................................ 9

3.2 Electrical Safety.......................................... 10

3.3 Safety Instructions

for Handling the Light Sources ................ 12

3.4 Notes on handling laser devices ............. 12

3.5 Safety Instructions

for Handling Acids and Bases.................. 12

3.6 Disposal........................................................ 13

4. Overview of the Leica DMI Series.......... 14

5. Unpacking the Microscope ..................... 27

6. Assembling the Microscope.................... 30

6.1 Assembly Tools........................................... 30

6.2 Installation of the Transmitted Light

Illumination Carrier (TL)............................. 31

6.3 Installation of the DIC Module

and DIC Objective Prisms ......................... 32

6.4 Installation of Specimen Stages.............. 33

6.5 Installation of Condensers........................ 38

6.6 Installation of Eyepieces........................... 43

6.7 Installation of Objectives .......................... 43

6.8 Installation of Filters

in the Illumination Arm............................... 44

6.9 Installing the transmitted Light

Lamp Housing ............................................. 44

Contents

8. Operation..................................................... 81

8.1 Switching on................................................ 81

8.2 Contrast Methods....................................... 83

8.2.1 Bright Field (TL)................................ 83

8.2.2 Phase Contrast (TL) ....................... 85

8.2.3 Dark Field (TL) .................................. 86

8.2.4 Polarization (TL)............................... 87

8.2.5 Differential

Interference Contrast (TL) ............. 88

8.2.6 Integrated Phase Contrast (TL)..... 89

8.2.7 Integrated

Modulation Contrast (TL)................ 90

8.3 Fluorescence............................................... 91

8.4 Combination Methods ............................... 93

8.5 Focusing....................................................... 94

8.6 Tubes ............................................................ 96

8.7 Port selection ............................................. 96

8.8 Eyepieces..................................................... 97

8.9 Objectives.................................................... 98

8.10 Stages and Object Displacement ............ 101

8.11 Magniﬁcation Changer.............................. 102

8.12 Light sources............................................... 103

8.13 Aperture and Field Diaphragm ................ 104

9. Troubleshooting.......................................... 105

10. Care of the Microscope ............................ 109

10.1 Dust Cover ................................................... 109

10.2 Cleaning ....................................................... 109

10.3 Handling Acids and Bases........................ 110

11. Major Consumable

and Replacement Parts............................. 111

12. Dimensions.................................................. 112

13. Abbreviations and Pictograms................ 113

14. Index ............................................................ 115

15. EU Declaration of Conformity .................. 117

1. Important Notes about this Manual

(1.2)

→p. 20

Numbers in parentheses, such as „(1.2)“, corre-

spond to illustrations (in the example, Figure 1,

Item 2).

Numbers with pointer arrows (for example

→ p. 20), point to a certain page of this manual.

Caution!

Special safety instructions within this manual

are indicated with the triangle symbol shown

here, and have a gray background.

Caution! The microscope and accessories can

be damaged when operated incorrectly.

Notes on the disposal of the microscope, ac-

cessories and consumable materials.

Explanatory note.

Item not contained in all conﬁgurations.

Text symbols, pictograms and their meanings:

Caution!

This operating manual is an essential com-

ponent of the microscope, and must be read

carefully before the microscope is assem-

bled, put into operation or used.

1. Important Notes about this Manual

This operating manual contains important in-

structions and information for the operational

safety and maintenance of the microscope and

accessories. It must therefore be kept safely for

future reference.

A separate manual is available on CD-ROM cov-

ering the operation of the Leica Application Suite

(LAS).

2. Intended Purpose of the Microscope

The Leica DMI Series microscopes covered in

this manual are designed for biological, routine,

and research applications. This includes the ex-

amination of samples taken from the human body

in order to provide information on physiological

or pathological states or congenital abnormali-

ties; to determine the safety and compatibility

with potential recipients; or to monitor therapeu-

tic measures.

The Leica DMI Series is an additional develop-

ment of Leica’s proven inverted research mi-

croscopes, designed for cellular and tissue

examination, micromanipulation and microinjec-

tion techniques, microdissection, and confocal

microscopy. The Leica DMI Series is suitable

for universal deployment. All contrast methods

such as dark ﬁeld, bright ﬁeld, phase contrast,

DIC, ﬂuorescence, and modulation contrast are

integral to the microscope and can be adapted

or changed quickly and easily. Variable illumi-

nation and imaging beam paths, as well as HCS

optics, modular accessories, and a comprehen-

sive range of peripherals complement the Leica

Microsystems inverted research stand.

The above-named microscope series complies

with the Council Directive 98/79/EEC concern-

ing in vitro diagnostics. They also conform to the

Council Directives 73/23/EEC concerning electri-

cal apparatus and 89/336/EEC concerning elec-

tromagnetic compatibility for use in an industrial

environment.

Caution!

The manufacturer assumes no liability for

damage caused by, or any risks arising from,

using the microscopes for purposes other

than those for which they are intended or not

using them within the speciﬁcations of Leica

Microsystems CMS GmbH.

In such cases the declaration of conformity

shall cease to be valid.

Caution!

These (IVD) devices are not intended for use

in the patient environment deﬁned by DIN

VDE 0100-710. Neither are they intended for

combining with medical instruments accord-

ing to EN 60601-1. If a microscope is electri-

cally connected to a medical instrument

according to EN 60601-1, the requirements

deﬁned in EN 60601-1-1 shall apply.

3. Safety Notes

3.1 General Safety Notes

This safety class 1 device is constructed and

tested in accordance with

EN 61010-2-101:2002,

EN 61010-1:2001,

IEC 61010-1:2001,

Safety regulations for electrical measuring, con-

trol, and laboratory devices.

Caution!

In order to maintain this condition and to en-

sure safe operation, the user must follow the

instructions and warnings contained in this

operating manual.

Caution!

The devices and accessories described in

this operating manual have been tested for

safety and potential hazards.

The responsible Leica afﬁliate or the main

plant in Wetzlar must be consulted whenever

the device is altered, modiﬁed or used in con-

junction with non-Leica components that are

outside of the scope of this manual.

Unauthorized alterations to the device or

noncompliant use shall void all rights to any

warranty claims!

3. Safety Notes

3.2 Electrical Safety

General Speciﬁcations

Leica CTR4000, CTR5000, CTR5500, CTR6000,

CTR6500, CTR7000, CTR6500 HS, CTR7000 HS

Electronics Boxes

For indoor use only.

Supply voltage:

Frequency:

Power input:

Fuses:

Ambient temperature:

Relative humidity:

Over voltage category:

Pollution degree:

Microscope

For indoor use only.

Supply voltage:

Frequency:

Power input:

Fuses:

Ambient temperature:

Relative humidity:

Over voltage category:

Pollution degree:

90–250 V~

50–60 Hz

max. 290 VA

T6.3 A

(IEC 60127-2/3)

15–35°C

max. 80% to 30°C

90–250 V~

50–60 Hz

see CTR4000–7000

15–35°C

max. 80% to 30°C

ebq 100 supply unit*

For indoor use only.

Supply voltage:

Frequency:

Power input:

Fuses:

Ambient temperature:

Relative humidity:

Over voltage category:

Pollution degree:

(see enclosed manual)

Leica EL6000*

For indoor use only.

Supply voltage:

Frequency:

Power input:

Fuses:

Ambient temperature:

Relative humidity:

Overvoltage category:

Pollution degree:

(see enclosed manual)

100–240 VAC

50–60 Hz

max. 200 VA

5x20, 2.5 A, slow,

breaking capacity H

0°–40°C

10–90%

non-condensing

90–250 V~

50–60 Hz

See CTR4000–7000 HS

15–35°C

max. 80% to 30°C

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