Leica MacroFluo Bedienungsanleitung

Leica

MacroFluo™

User manual

Leica MacroFluo – User manual

Dear User,

Thank you for your faith in our product. We hope your work with it is both

enjoyable and successful.

Leica MacroFluo™ with zoom 6.3:1 or 16:1 are worldwide the only macro docu-

mentation systems for fluorescence. The MacroFluo™ concept combines the

advantages of macroscopy – large fields of view, large working distances,

parallax-free and precise imaging – with fluorescence technology (including

multi color fluorescence) at a high resolution. For this purpose, we combined the

excellent optics of the apochromatic zoom systems Leica Z6 APO (6.3:1 Zoom)

or Z16 APO (16:1 Zoom) with the brilliant Leica fluorescence technology. The

Leica MacroFluo™ systems are the correct choice to examine and digitally

detect the effects of a gene defect on living transgenic models from the size

of a whole mouse at highest precision and resolution in an intensively fluores-

cent image field.

In developing our instruments, we place great value on simple, self-explanatory

operation. However, please take the time to read the user manual and the oper-

ating safety information to become familiar with the advantages and options of

your Leica MacroFluo™, so that you can use them optimally and safely.

Should you have any questions, please consult your local Leica representative.

You will find the address of the closest local representative as well as valuable

information about products and services from Leica Microsystems on our home-

page at www.leica-microsystems.com

We are gladly at your service. Customer service is a big thing with us – before

and after the sale.

Leica Microsystems (Switzerland) Ltd.

Stereo & Macroscope Systems

www.stereomicroscopy.com

The Leica MacroFluo™ systems were developed in conjunction with the

Imaging Centre of IGBMC.

3Leica MacroFluo – Overviews

Overview of the instrument

Fig. 1 Leica MacroFluo™ with zoom system 16:1

1Base of stand (transmitted-light base HL RC™

shown)

2Focusing drive with column (shown with motor

focus) and focusing stop (shown with holder for

fiber-optic light guide)

3Handheld controller (only if fitted with motor

focus)

4Gliding stage (optional), glass stage plate is

standard

5Zoom system 6.3:1 or 16:1 (Z16 APO shown

with 16:1 zoom)

6Objective of your choice

7Carrier for the incident illuminator

8Coaxial incident light fluorescence illuminator

LRF 4/22

9Lamp housing 106 z with 100 W high-pressure

mercury burner

10 Trinocular tube (FSA HC L 3TP 4/5/7 shown)

11 Wide-angle HC plan eyepieces 10×/22 or 10×/25

12 TV adapter of your choice

13 CCD camera of your choice (Leica DFC300 FX

shown)

14 UV protection screen with arm

15 Supply unit Hg 100W and 103 W/2

Page

1 Safety concept . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.1 The user manual . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.1.1 Diagrams . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.1.2 Symbols used . . . . . . . . . . . . . . . . . . . . . . . 6

1.2 Safety instructions . . . . . . . . . . . . . . . . . . . . . . . . 6

1.3 Light source: Safety instructions . . . . . . . . . . . 9

2 Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

2.1 Expansion diagram . . . . . . . . . . . . . . . . . . . . . . 10

2.2 Assembly sequence . . . . . . . . . . . . . . . . . . . . . 11

2.3 Stand assembly . . . . . . . . . . . . . . . . . . . . . . . . . 11

2.4 Incident light fluorescence illuminator . . . . . 11

2.5 Trinocular tube . . . . . . . . . . . . . . . . . . . . . . . . . . 13

2.6 Zoom system . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

2.7 Objectives . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

2.8 UV protection screen . . . . . . . . . . . . . . . . . . . . 15

2.9 Lamp housing . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

2.10 Instrumentation of nosepiece disc . . . . . . . . 16

2.11 Connection of digital or video cameras . . . . 18

2.12. Electrical connections . . . . . . . . . . . . . . . . . . 18

3 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

3.1 Starting up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

3.1.1 Transmitted light or incident

light observation . . . . . . . . . . . . . . . . . . . . 19

3.1.2 Incident light fluorescence . . . . . . . . . . . 19

3.1.3 Disconnecting from the power supply . 19

3.2 Trinocular tube . . . . . . . . . . . . . . . . . . . . . . . . . . 19

3.3 Zoom . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

3.4 Iris diaphragm . . . . . . . . . . . . . . . . . . . . . . . . . . 21

3.5 Focusing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

3.5.1 Fine focusing . . . . . . . . . . . . . . . . . . . . . . .21

3.6 Microscopy with/without glasses . . . . . . . . . . 21

3.7 Adjusting diopter settings . . . . . . . . . . . . . . . . 22

3.8 Incident light fluorescence illuminator . . . . . 24

3.8.1 Field diaphragm . . . . . . . . . . . . . . . . . . . . 24

3.8.2 Aperture diaphragm . . . . . . . . . . . . . . . . . 24

3.8.3 Light stop . . . . . . . . . . . . . . . . . . . . . . . . . . 25

3.8.4 Filter BG38 . . . . . . . . . . . . . . . . . . . . . . . . . 25

3.8.5 Filter blocks . . . . . . . . . . . . . . . . . . . . . . . . 25

4 Appendix . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

4.1 Filter blocks for macro-fluorescence . . . . . . . 26

4.2 Assignment of fluorochromes

and filter blocks . . . . . . . . . . . . . . . . . . . . . . . . . 27

4.3 Optical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

4.4 Technical data, performance

characteristics . . . . . . . . . . . . . . . . . . . . . . . . . . 32

4.5 Dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

Leica MacroFluo – User manual

Contents

Leica MacroFluo – User manual

1. Safety concept

1.1 The user manual

Included with your Leica MacroFluo™ is an inter-

active CD-ROM with all pertinent user instructions

in German, English, French, Spanish, Italian, Por-

tuguese, Dutch, Danish, Swedish, Finnish, and

Greek. Keep it in a safe place, and readily acces-

sible to the user. User manuals and updates are

also available for you to download and print from

our web site at www.stereomicroscopy.com.

This operating manual describes the special

functions of the Leica MacroFluo™ system and

contains important instructions for their opera-

tional safety, maintenance, and accessories.

Leica MacroFluo™ systems feature a modular

design. The following components are described

in special user instructions on the supplied

CD-ROM:

– Leica Z6 APO and Z16 APO zoom systems:

Operating instructions M2-416-0

– Lamp housing with high-pressure mercury

burner: Operating instructions M2-216-1

– Supply unit ebq 100 isolated for 100-W high-

pressure mercury burner.

– Incident light fluorescence illuminator

– Operating instructions M2-105-0 M series

– Motor focus system: Operating instructions

M2-267-1

– Leica transmitted-light base HL RC™: Operating

instructions M2-216-2

These operating instructions are an

essential component of the instrument. Carefully

read these operating instructions and those listed

above before assembly, startup and use. In partic-

ular, please observe all safety instructions.

To maintain the unit in its original condition and

to ensure safe operation, the user must follow the

instructions and warnings contained in these

user manuals.

1.1.1 Diagrams

(1.8)

Numbers in parentheses within the descriptions

pertain to the numbers of the diagrams and the

items in the diagrams.

Example (1.8): Figure 1 is found on Page 3 and item

8 is the coaxial incident illuminator.

Leica MacroFluo – General notes

1.1.2 Symbols used

Warning of a danger

This symbol indicates especially important infor-

mation that, if not observed,

– can cause hazards to personnel,

– can lead to functional disturbances and

damaged instruments.

Warning of hazardous electrical voltage

This symbol indicates especially important infor-

mation that, if not observed,

– can cause hazards to personnel,

– can lead to functional disturbances and dam-

aged instruments.

Danger due to hot surface.

This symbol warns against touching hot surfaces,

e.g. those of light bulbs.

Important information

This symbol indicates additional information or

explanations that intend to provide clarity.

Action

왘This symbol refers to actions described in the

text that are to be carried out.

Explanatory notes

• This symbol indicates additional notes and

explanations provided in the text.

1.2 Safety instructions

Description

Leica MacroFluo™ systems are macroscopes

with vertical beam path for precise fluorescence

applications. Leica MacroFluo™ systems are

modular and can be combined individually. A

MacroFluo™ equipment consists of:

– a zoom system of your choice with an objective

of your choice, poss. fine focusing

– the carrier for the incident illuminator

– the incident light fluorescence illuminator

– filter blocks of your choice

– the lamp housing with 100-W high-pressure

mercury burner, stray-light protection and

supply unit

– the UV protection screen with arm and clamp

– a trinocular tube of your choice

– two eyepieces of your choice

– a stand base of your choice

– a focusing drive of your choice

– a high-performance digital camera with

photo/video adapters of your choice

Documents, unpacking

Please carefully compare your supply with the

packing slip, delivery note or invoice. We recom-

mend to keep a copy of these documents together

with the operating instructions. Please ensure

that no small parts are left in the packaging mate-

rial when unpacking.

In accordance with our guidelines (to ISO 14001),

we are using packaging material that allows for

environmentally-friendly recycling, as well as bub-

ble wrap that corresponds to the latest technical

standards (RESY).

Do not connect any devices to the supply

system at this time.

Permitted uses

The Leica MacroFluo™ allows for examining,

manipulating, sorting and documenting entire

unprepared fluorescent objects. The high resolu-

tion, high light utilization and specially selected

filter blocks allow for distinguishing the finest

structures and increase the richness of informa-

tion with incident light fluorescence. But the

Leica MacroFluo™ can also be used to perform

observations without fluorescence in transmitted

light or incident light.

Non-intended use

If the Leica MacroFluo™, its components or

accessories are used other than as described in

the user manual, injury or property damage may

occur. Never:

– install other plugs or cables.

– change, rebuild or take apart parts, if not

specifically instructed to in this manual.

– allow unauthorized persons to open parts.

– use the Leica MacroFluo™ for examinations

and operations involving the human eye.

The devices and accessories described in this

operating manual have been tested for safety and

potential hazards. The responsible Leica affiliate

or the Leica headquarters in Wetzlar must be con-

sulted whenever the device is altered, modified or

used in conjunction with non-Leica components

that are outside of the scope of this manual!

Unauthorized alterations to the device or noncom-

pliant use shall void all rights to any warranty

claims!

Place of use

– The Leica MacroFluo™ may only be operated

in closed, dust-free rooms at +10 ˚C to +40 ˚C.

– Ensure that the room is free of oil and other

chemical fumes and extreme humidity.

– Electrical components must be assembled at

least 10cm away from the wall and from flam-

mable substances.

– Large temperature fluctuations, direct sunlight

and vibrations should be avoided. These condi-

tions can distort measurements and micro-

graphic images.

– Optical instruments in warm and warm-damp

climatic regions require special care in order

to prevent the build up of fungus.

Responsibilities of the person(s) in charge of

instrument

Ensure that

– the Leica MacroFluo™ and accessories are

operated, maintained and repaired by autho-

rized and trained personnel only.

– the operating personnel has read, understands,

and is observing these instructions as well as

those listed on page 5, and, in particular, the

safety instructions.

Transport

– Use the original packaging for shipping or

transporting the Leica MacroFluo™ and their

accessory components.

– In order to prevent damage from vibrations,

disassemble all moving parts that (according to

the user manual) can be assembled and disas-

sembled by the customer and pack them sepa-

rately. These include the objective, trinocular

tube, eyepieces, lamp housing, burner, etc.

Leica MacroFluo – General notes

8Leica MacroFluo – Assembly

Repairs and servicing

– Only Leica Microsystems-trained service tech-

nicians, or technical specialists assigned by the

person in charge of the instrument, are permit-

ted to carry out repairs.

– Only original Leica Microsystems spare parts

may be used.

– Unplug the power cable, the power supply or

the supply unit before opening voltage carrying

parts such as the Leica MacroFluo™.

Touching the live circuit can cause injury.

Integration in third-party products

When installing Leica products into third-party

products, note the following: The manufacturer of

the complete system or its dealer is responsible

for following all applicable safety instructions,

laws and guidelines.

Electrical connections

Observe the following notes and warnings to

ensure a perfect safety-related condition of the

devices:

– Connect the power plug only to a grounded

power outlet.

– Contact the service in case of systems without

protective conductor.

– The protective action must not be canceled by

using an extension cable without protective

conductor.

Disposal

– The products described here must be disposed

off in accordance with applicable local laws

and regulations.

– High-pressure mercury burners must be dis-

posed of as hazardous waste.

Legal requirements

Adhere to general and local regulations relating

to accident prevention and environmental protec-

tion.

Conformity with European Community

directive

The Leica MacroFluo™ and their accessories are

constructed based on the state of the art of tech-

nology and are provided with an EU Declaration of

Conformity.

Health risks

The ergonomic optics concept and the design of

Leica instruments aim to limit the strain on the

user to the lowest possible level.

Workplaces with optical systems facili-

tate and improve the viewing task, but they also

impose high demands on the eyes and holding

muscles of the user. Depending on the duration

of uninterrupted work, asthenopia and muscu-

loskeletal problems may occur. For this reason,

appropriate measures for reduction of the work-

load must be taken:

– A basic requirement is a stable stage at optimal

height (70 – 80cm). Together with an ergonomi-

cally correct, multi-adjustable chair, these are

the external requirements for fatigue-free

microscopy work.

– optimal arrangement of workplace, work

assignments and work flow (changing tasks

frequently).

– thorough training of the personnel, giving con-

sideration to ergonomic and organization

aspects.

– when assembling or disassembling the lamp

housing,

– before opening the lamp housing,

– when changing the high-pressure mercury

burner and remaining parts, such as the heat-

absorbing filter or the collector,

– for service work on the supply unit.

Lamp housing

Leica MacroFluo™ with fluorescence illuminator

may be used only with the Leica lamp housing

106 z for 100-W mercury bulb, supply unit and

stray-light protection.

For assembly work, always pull the

power plug of the supply unit from the socket!

Before inserting the burner, disconnect the power

supply connection. Adjust the burner immediately

after ignition. See also the special guideline.

Leica Microsystems assumes no liability

for damages if bulbs from other manufacturers

are used.

– Minimum distance of lamp housing (hot sur-

faces!) 10cm (4") from flammable objects such

as books, curtains, wallpaper. Fire hazard!

– Never look into the direct beam path! Danger of

blinding!

– Never open the lamp housing when the lamp is

switched on. Disconnect the power plug of the

supply unit from the supply system.

Danger of explosion, UV radiation, danger of

blinding!

– Let the lamp housing cool off for at least 15 min.

Danger of explosion, danger of burns.

– Never cover the venting slots on the lamp hous-

ing. Fire hazard!

– Use stray-light protection.

UV radiation can damage the eyes.

Direct contact with eyepieces is a poten-

tial transmission method for bacterial and viral

infections of the eye. Users should be made

aware of the potential risk of infection. The risk

can be kept to a minimum by using personal eye-

pieces for each individual or detachable eyecups.

1.3 Light source: Safety instructions

Protective measures of the manufacturer:

– UV protection screen in front of the object

plane prevents direct view onto the UV beams.

– The design prevents direct UV radiation on the

eyes in case of empty positions of the nose-

piece disc.

– UV filter in the observation beam paths protects

the eyes.

– Stray-light protection on the lamp housing pre-

vents irradiation of the hands.

Warning: UV radiation could damage

the eyes.

For this reason:

– never look at the light spot on the object plane

without UV protection screen.

– do not select a white, heavy reflecting back-

ground for the objective.

– Never open the lamp housing while the lamp is

switched on.

Danger of explosion, UV radiation, danger of

blinding!

Supply unit

Do not connect the unit until the lamps have been

assembled. Check the setting of the power supply

voltage and correct it, if necessary. See also the

special guideline.

Always disconnect the power plug of the supply

unit from the supply system:

Leica MacroFluo – Assembly

10 447 041 (500mm)

11 507 808

10 445 363

10 445 631

10 445 367

10 446 359

10 447 185 (500 mm)

10 447 057

10 446 301

10 445 387

10 446 100 (500mm)

Z6 APO

10 447 174

Z16 APO

10 447 173

0.5x

10 447 177

10 447 176

10 447 178

Vario TV-Adapter, delta

11 541 038

0.55x - 1.1x

11 543 702

11 543 706

B-Mount

2/3”

B-Mount

1/2”

C-Mount

11 543 703

0.8x

10 446 360

10 447 163

10 446 182

10 399 211

10 445 654

10 446 271

11 504 069

10 446 228

10 447 279

EPQ100

11 500 325

11 505 065

11 551 505 / 11 501 598

10 445 154

Filter

11 513 841

11 513 843

11 513 837

11 513 844

11 513 845

11 513 847

11 513 863

11 513 864

11 513 862

11 513 842

MacroFluo

11 513 804

11 513 839

11 513 805

11 513 806

11 513 807

11 513 808

11 513 809

11 513 840

11 513 811

11 513 812

11 541 512

0.35x

11 541 511

0.5x

11 541 537

0.63x

11 541 510

C-Mount

11 541 517

0.32x - 1.6x

0.55x - 1.1x

MPV-Adapter,

HC/delta

11 621 500

10 447 178

10 447 175

2. Assembly

2.1 Expansion diagram

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