Leica DM6000 M Bedienungsanleitung

Leica DM6000B

Leica DM6000M

Operating Manual

Published January 2004 by:

Leica Microsystems Wetzlar GmbH

Ernst-Leitz-Straße

D-35578 Wetzlar (Germany)

Responsible for contents:

Karin Schwab

(Marketing CM, Compound Microscopy, Product Management)

Holger Grasse

(Safety Officer according to MPG §30)

In case of questions, please contact the hotline: Phone +49(0)6441-292286

Fax +49(0)6441-292255

E-mail: [email protected]

Leica DM6000B

Leica DM6000M

Operating Manual

Copyrights

All rights to this documentation are held by Lei-

ca Microsystems Wetzlar GmbH. Reproduction

of text or illustrations (in whole or in part) by

print, photocopy, microfilm or other method (in-

cluding electronic systems) is not allowed with-

out express written permission from Leica

Microsystems Wetzlar GmbH.

The term "Windows" can be used in the follow-

ing text without further identification. It is a reg-

istered trademark of the Microsoft Corporation.

Otherwise, no inference with regard to the free

usability of product names may be drawn from

the use of those names.

The instructions contained in the following

documentation reflect state-of-the-art technol-

ogy and knowledge standards. We have com-

piled the texts and illustrations as accurately as

possible. Nevertheless, no liability of any kind

may be assumed for the accuracy of this manu-

al’s contents. Still, we are always grateful for

comments and suggestions regarding potential

mistakes within this documentation.

The information in this manual is subject to modifi-

cation at any time and without notification.

Contents

7. Startup ........................................................ 38

7.1 Functional Principle ................................. 38

7.2 Switching on .............................................. 43

7.3 The LeicaScreen ....................................... 44

7.4 The Function Keys at the Stand ............. 45

7.5 The Remote Control Element

SmartMove ................................................ 46

7.6 Köhler Illumination ................................... 46

7.6.1 Transmitted Light ........................... 46

7.6.2 Incident Light .................................. 48

7.7 Checking Phase Contrast Rings ............. 49

7.8 Adjusting the motorized Polarizer ......... 51

7.9 Adjusting the Light Sources ................... 51

8. Operation ................................................... 57

8.1 Switching on .............................................. 57

8.2 Stages and Specimen Displacement .... 59

8.3 Focusing ..................................................... 60

8.4 Tubes ..........................................................

8.5 Eyepieces ................................................... 63

8.6 Objectives .................................................. 63

8.7 Magnification Changer ............................ 66

8.8 Light Sources............................................ 66

8.9 Aperture Diaphragm and

Field Diaphragm ........................................ 67

Contents

1. Important Notes about this Manual ..... 7

2. Intended Purpose of Microscopes ....... 8

3. Safety Notes .............................................. 9

3.1 General Safety Notes............................... 9

3.2 Electrical Safety........................................ 10

4. Overview of the Instrument.................... 12

5. Unpacking the Microscope.................... 18

6. Assembling the Microscope.................. 20

6.1 Stage ........................................................... 21

6.2 Condenser .................................................. 22

6.3 Tube and Eyepieces ................................. 23

6.4 Objectives .................................................. 24

6.5 Light Sources

for the Transmitted Light Axis ................ 24

6.6 Light Sources

for the Incident Light Axis ....................... 26

6.7 Equipping the

Incident Light Turret Disc ....................... 31

6.8 Polarizer and Analyzer............................. 32

6.9 DIC Prisms.................................................. 33

6.10 Optional Accessories............................... 34

6.11 Connecting

the Leica CTR6000 Electronics Box....... 36

6.12 Connecting the Computer ....................... 37

6.13 Connection to the Power Supply ........... 37

Contents

9. Contrasting Methods

for Leica DM6000 B .................................. 68

9.1 Transmitted Light...................................... 68

9.1.1 Bright Field...................................... 68

9.1.2 Phase Contrast ............................... 69

9.1.3 Dark Field......................................... 69

9.1.4 Polarization ..................................... 70

9.1.5 Differential

Interference Contrast.................... 71

9.2 Fluorescence ............................................. 72

9.3 Combi Mode ............................................... 73

10. Contrasting Methods

for Leica DM6000 M ................................. 74

10.1 Incident Light ............................................. 74

10.1.1 Bright Field...................................... 74

10.1.2 Dark Field......................................... 74

10.1.3 Polarization ..................................... 75

10.1.4 Interference Contrast.................... 76

10.1.5 Fluorescence .................................. 76

10.2 Transmitted Light...................................... 77

10.2.1 Bright Field...................................... 77

10.2.2 Polarization ..................................... 77

11. Trouble Shooting ...................................... 78

12. Care of the Microscope .......................... 81

12.1 Dust Cover.................................................. 81

12.2 Cleaning...................................................... 81

12.3 Handling Acids and Bases ...................... 82

13. Essential Wear and Spare Parts .......... 83

14. Abbreviations and Pictograms.............. 84

15. Index ........................................................... 86

16. EU Declaration of Conformity ................ 87

1. Important Notes about this Manual

(1.2)

→

p.20

Numbers in parentheses, such as "(1.2)", corre-

spond to illustrations (in the example, Figure 1,

Item 2).

Numbers with pointer arrows (for example

→ p.20), point to a certain page of this manual.

Caution!

Special safety instructions within this

manual are indicated with the triangle

symbol shown here, and have a gray

background.

Caution! The microscope and accessories can

be damaged when operated incorrectly.

Explanatory note.

Item not contained in all configurations.

Text symbols, pictograms and their meanings:

Caution!

This operating manual is an essential com-

ponent of the microscope, and must be read

carefully before the microscope is

assembled, put into operation or used.

1. Important Notes about this Manual

This operating manual contains important in-

structions and information for the operational

safety and maintenance of the microscope and

accessories. Therefore, it must be kept and

taken care of.

For the operation of the LeicaScreen and the

Software Leica DMControl, please see separate

operating manual.

2. Intended Purpose of Microscopes

The Leica DM6000 B microscope, to which this

user manual belongs, is designed for biological

routine and research applications. This includes

the examination of samples taken from the hu-

man body with a view to providing information

on physiological or pathological states or

congenital abnormalities, or to determining the

safety and compatibility with potential recipients,

or to monitoring therapeutic measures.

The Leica DM6000 M microscope is designed

for examinations in the field of materials

research.

All the above-named microscopes comply with

the Council Directive 98/79/EEC concerning in

vitro diagnostics. They also conform to the

Council Directives 73/23/EEC concerning

electrical apparatus and 89/336/EEC concerning

electromagnetic compatibility for use in an

industrial environment.

Caution!

The manufacturer assumes no liability for

damage caused by, or any risks arising from

using the microscopes for other purposes

than those for which they are intended or

not using them within the specifications of

Leica Microsystems Wetzlar GmbH.

In such cases the conformity declaration

shall cease to be valid.

Caution!

These (IVD) devices are not intended for use

in the patient environment defined by DIN

VDE 0100-710. Neither are they intended for

combining with medical devices according

to EN 60601-1. If a microscope is electrically

connected to a medical device according to

EN 60601-1, the requirements defined in

EN 60601-1-1 shall apply.

3. Safety Notes

3.1 General Safety Notes

This safety class 1 device is constructed and

tested in accordance with

EN 61010-2-101:2002,

EN 61010-1:2001,

IEC 1010-1:2001,

safety regulations for electrical measuring, con-

trol, and laboratory devices.

Caution!

In order to maintain this condition and to en-

sure safe operation, the user must follow the

instructions and warnings contained in this

operating manual.

Caution!

The devices and accessories described in

this operating manual have been tested for

safety and potential hazards.

The responsible Leica affiliate or the main

plant in Wetzlar must be consulted when-

ever the device is altered, modified or used

in conjunction with non-Leica components

that are outside of the scope of this manual.

Unauthorized alterations to the device or

noncompliant use shall void all rights to any

warranty claims!

3. Safety Notes

Caution!

The power plug may only be plugged into an

outlet equipped with a grounding contact.

Do not interfere with the grounding function

by using an extension cord without a ground

wire. Any interruption of the ground wire in-

side or outside of the device, or release of

the ground wire connection, can cause the

device to become hazardous. Intentional

ground interruption is not permitted!

Caution!

Through activating to the grounding

connection (earth screw on the back of the

Leica CTR6000 Electronics Box) ancillary

equipment with its own and/or extra power

supply may be brought to the same ground

wire potential. For connections without a

ground connector, Leica Service must be

consulted.

Caution!

Never use any fuses as replacements other

than those of the types and the current rat-

ings listed here. Using patched fuses or

bridging the fuse holder is not permitted.

3.2 Electrical Safety

General specifications

Leica CTR6000 electronics box

For indoor use only.

Supply voltage:

Frequency:

Power input:

Fuses:

Ambient temperature:

Relative humidity:

Overvoltage category:

Pollution degree:

Microscope

For indoor use only.

Supply voltage:

Frequency:

Power input:

Fuses:

Ambient temperature:

Relative humidity:

Overvoltage category:

Pollution degree:

Supply unit ebq 100*

For indoor use only.

Supply voltage:

Frequency:

Power input:

Fuses:

Ambient temperature:

Relative humidity:

Overvoltage category:

Pollution degree:

(see enclosed manual)

90-250 V~

50-60 Hz

max. 290 VA

T6,3 A

(IEC 60127-2/3)

15-35°C

max. 80% to 30°C

90-250 V~

50-60 Hz

See CTR6000

15-35°C

max. 80% to 30°C

90-250 V~

50-60 Hz

max. 155 VA

2xT2A (IEC 127)

10-36°C

max. 80% to 30°C

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