Motic SFC-3 Series Bedienungsanleitung

Instruction Manual

SFC-3 Series

Motic Microscopes, European Division

2. Eyepiece

holder tube

10. Fine focus

knob

9. Coarse focus

knob

12. Arm

8. Diaphragm

6. Stage clips

7. Stage

5. Objectives

4. Revolving

nosepiece

1. Eyepiece

3. Head

11. Head

adjustment

screw

13. Mirror

14. Base

SFC-3AF

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Introduction

Thank you for your purchase of a Motic microscope. Motic microscopes are precision instruments, subjected

to meticulous examination in order to reach you in perfect condition. Their design combines easy

management and optimum functioning with minimum maintenance.

The information contained in this manual is likely to go beyond what the average user needs to know to use

the microscope, however, it is provided to answer any queries that may arise.

Your new microscope combines high performance features, with an excellent degree of optical resolution

and clarity of image. It incorporates a 84mm x 88mm stage with objectives located on a ball bearing

nosepiece; a precision focusing system; a disk diaphragm with six holes and a plano-concave mirror.

These instructions should be read carefully before operating the microscope. They will permit you to use

your new microscope to its fullest capabilities. Terminology used to describe components and controls can

be found in the diagram on page 2.

These instructions are based on the assembly and use of the SFC-3AF model with additional notes

applying specifically to other models in the series.

Unpacking

All components of the microscope have been carefully packed to make sure they reach you in perfect

condition. We recommend that you do not discard any packing containers in case you need to return the

microscope, store it for long periods of time; or should it become necessary to transport it to a technical

service for any repair or maintenance procedure.

The box should contain the following components, depending on the model:

•SFC-3A: A microscope with a monocular vertical head with an eyepiece, coarse focusing system,

stage with diaphragm and three objectives. A dust cover is also provided.

•SFC-3AF: A microscope with a monocular vertical head with an eyepiece, coarse and fine focusing

system, stage with diaphragm and three objectives. A dust cover is also provided.

•SFC-3BFR: A microscope with a monocular inclined rotating head with an eyepiece, coarse and fine

focusing system, stage with diaphragm and three objectives. A dust cover is also provided.

Remove, and handle the microscope and all its components with extreme care.

Avoid touching the lenses of the optical elements and keep clear of contact with dust, water or other

contaminating agents, as they could stain or damage the lens surface and affect the quality of the

image.

A. Place the microscope in an upright position on a clean flat and stable surface.

B. Remove the rest of the components from the box.

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Description of components

1. Head (3). Monocular vertical. For the model SFC-3BFR monocular inclined 45ª and 360º rotating.

2. Eyepiece (1). The lenses closest to the eye, they magnify the image formed by the objectives.

3. Revolving nosepiece (4). Permits the change in magnification by rotation, the correct positioning of

the objectives is marked by a “click” in the optical path

4. Objectives (5). The group of lenses closest to the sample, or microscopic specimen forming the first

magnified image.

5. Stage (7). Platform of the microscope where the specimen is placed. The specimen slide is held in

place by specimen holder clips.

6. Diaphragm (8). Optimises illumination for enhanced resolution and image contrast.

7. Focusing Knobs (9-10). Situated on both sides of the arm of the microscope, the larger or coarse

focusing knob initially brings the specimen into focus, and the smaller or fine focusing knob permits a

precise adjustment of the image. (Model SFC-3A does not include fine focus knob)

8. Plano-concave mirror (13). Proportions the illumination on the sample.

Operating

A. Illumination.

1. Place the microscope near a light source, or a window.

2. Place the microscope in the most comfortable position for use.

3. Looking through the eyepiece, adjust the mirror so that the light is reflected, and illuminates

the field of vision.

WARNING: DO NOT USE DIRECT SUNLIGHT AS A LIGHT SOURCE

B. Focussing the microscope.

1. Turn the revolving nosepiece (4) and place the 4X objective (5) in the optical path making

sure that it clicks into the correct position.

2. Turn the coarse focus knob (9) until the head (3) is in its highest position.

3. Place a microscopic sample on the stage (7), making sure that the cover slip is facing

upwards. Lift the specimen holder clips (6) pressing downwards on the lower part, and slide

the specimen slide under the clips. Release pressure so that the slide is firmly held in place.

4. Make sure that the sample on the slide is in the optical path.

5. While looking through the eyepiece (1), turn the coarse focus knob until the sample appears

in focus.

6. Readjust the focus with the fine focus knob (10) until the image appears sharp. (Model SFC-

3A does not include a fine focus knob).

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C. Adjusting the opening on the diaphragm.

The diaphragm (8) for this series consists of a rotating disk with 6 apertures of distinct

diameters. A click marks the correct positioning in the optical path.

The function of the diaphragm is to obtain the best resolution and to provide contrast in the

image. Smaller apertures will deliver higher contrast to image, although if the aperture is too

small resolution will be poor. It is best to experiment to achieve the best resolution possible.

Suggested apertures for each objective are as follows:

OBJECTIVE APERTURE OF IRIS

4X The largest aperture.

10X The medium aperture.

40X The smallest aperture.

D. Changing magnification.

1. Position the 10X objective (5) in the optical path.

2. This microscope has been parfocalised by the manufacturer, although it is possible that

small differences exist between objectives. The fine focus knob (10) can be used to readjust

the focus. For the model SFC-3A adjust using the coarse focus knob (9).

3. When changing to the 40X take care to ensure that the objective does not rub against the

slide, as damage can occur to the front lens of the objective.

Maintenance

A. Optical maintenance.

Do not attempt to disassemble any optical component.

Prior to cleaning any of the lens surfaces, remove dust particles using a fine brush, specifically for

cleaning lenses. Alternatively, use low pressure compressed air, available in shops selling

photography equipment.

1. Cleaning the eyepiece

a. Do not remove the eyepiece (1) or the eyepiece tube (2).

b. Only clean the outer surface, misting the lens with breath.

c. Afterwards, dry the lens with special lens paper in circular motions, from the centre

outwards. Do not clean the lenses when dry as they can be easily scratched.

2. Cleaning the objectives.

a. Do not remove the objectives (5) from the microscope.

b. Only clean the outer surface. Use a soft cotton cloth slightly dampened with Xylene,

afterwards dry the lens with the same cloth.

3. Cleaning the mirror.

Use any of the above methods mentioned for cleaning eyepiece or objectives.

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B. Mechanical Maintenance

4. Adjusting the tension of the coarse focus knob.

The collar to adjust the tension of the coarse focus knob (Fig. 1) is situated between

the coarse focus knob (9) and the arm (12).

The tension of the coarse focus is pre adjusted by the manufacturer. The optimum

tension point is that which permits the coarse focus knob to move as smoothly as

possible, without the head (7) sliding down by itself.

a. To adjust tension first loosen the screw situated in one of the four orifices of the

collar with a 2mm hexagonal key.

b. To tighten the tension of the coarse focus knob, turn the collar in the clockwise. To

loosen it, turn anti clockwise.

c. Tighten hexagonal screw.

5. Adjusting the rack stop.

The 40X objective use a retractable

security system to avoid damage to the

slide or to the front of the lens should they

come into contact. An additional security

measure is the security rack stop screw

that regulates the descending movement

of the head. It is adjusted by the

manufacturer for the observation of

standard slides with a cover slip 0.17mm

thick; but for the observation of other types

of slides, readjustment may be necessary.

Tensión

adjustment

collar

Set screw

Screw used to

adjust the height

of the head

a. Firstly, loosen the rack stop

screw. (Fig. 1)

b. Next, unscrew the adjustment

screw. (Fig. 1)

c. Turn the fine focus knob (10)

until at mid point (not applicable

for SFC-3A).

Fig. 1

d. Focus on the slide only using the coarse focus control (9) with the 4X objective (5)

firstly, and then the 10X.

e. Tighten the adjustment screw only until firm, so that the head (3) cannot descend.

f. Tighten the rack stop screw.

-6-

Troubleshooting

IMAGE QUALITY

PROBLEM CAUSE SOLUTION

No image. Nosepiece not positioned properly.

Image too bright. Turn until clicks into place.

Reduce the intensity of the light.

Poor resolution. Dirty objective.

Dirty eyepiece.

Slide upside down.

Wrong cover slip used with slide.

Light too bright.

Dirty condenser.

Clean objective.

Clean eyepiece.

Replace the slide with the cover slip

facing upwards.

Use 0.17mm thick cover slips.

Reduce light intensity or adjust the

diaphragm aperture.

Clean condenser.

Spots in field of view Dirty eyepiece.

Dirty slide.

Dirty condenser.

Clean eyepiece.

Clean preparation.

Clean condenser.

Uneven illumination of field. Nosepiece not positioned properly.

Diaphragm aperture not sufficiently

open.

Turn until clicks into place.

Adjust appropriately.

MECHANICAL

PROBLEM CAUSE SOLUTION

Does not stay in focus. The head is sliding down on its own. Adjust the tension of the coarse focus

knob.

Does not focus. The stopper on the ascending

movement of the stage needs

adjusting. Readjust the stopper.

Moving the microscope

•Avoid moving the microscope if possible.

•Carry the microscope in both hands, with one hand holding the arm (12), and the other supporting the

base (14).

•Keep the microscope in an upright position.

Repairs

If the microscope needs repairing, or revision by authorised personnel, we would recommend that it be

stored in its polystyrene box and returned to the distributor. Attach a note with a description of the problem,

or details of the required revision.

Warrantee

All MOTIC microscopes are warranted against any manufacturing defect for a 5 year period. Damage

occurring by any unauthorised repair work, or occurring through misuse or modification of the microscope will

not be included under the conditions of the warrantee. Bulbs and fuses are not under warrantee.

The warrantee service is provided by MOTIC, or its authorised distributors. Defective products will be

repaired without charge when returned to MOTIC, or one of its distributors. Transport costs will be covered

by the purchaser.

OWING TO POSSIBLE MODIFICATIONS AND IMPROVEMENTS IN THEIR MANUFACTURE, CHANGES

MAY OCCUR TO MICROSCOPES WITHOUT PRIOR NOTICE.

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