Nikon OPTIPHOT-POL Bedienungsanleitung

Nikon

Polarizing Microscope

OPTIPHOT -POL

INSTRUCTIONS

NIPPON KOGAKU K. K.

CAUTIONS

oAvoid sharp knocks!

Handle the microscope gently, taking care

to avoid sharp knocks.

f}When carrying the microscope

When carrying the microscope, hold its arm

with one hand, supporting the bottom of

the microscope base with the other. The

instrument weighs about 10.5 kg. Do not

have the lamp housing carry any load.

8Place for using

Avoid the use of the microscope in a dusty

place, where it is subject to vibrations or

exposed to high temperatures, moisture or

direct sunlight.

oPower source voltage

In every case, make sure of the power

source voltage by means of the input

voltage change-over switch on the bottom

of the microscope base.

oLight source

Halogen lamp bulb to be used is 12V-

50W. Do not use 12V-l OOWhalogen lamp

bulb. If the lamp bulb of over-rated wat-

tage is used, light adjusting circuit will

damage.

Never connect the lamp housing cord to

the house current socket directly.

oIn lighting the lamp

Take care not to touch the lamp housing

being lighted, and don't bring inflammable

substances such as gasoline, thinner, and

alcohol near to the lamp housing, as some

parts of the lamp housing may take a high

temperature while the lamp is being light-

ed.

oExchanging the lamp bulb and fuse

Before replacing the lamp bulb or fuse,

turn 0 F F the power switch and disconnect

the plug of the power source cord.

In such cases as of replacement, do not

touch the lamp bulb with bare hands, im-

med iately after putting out the lamp.

«;) Dirt on the lens

Do not leave dust, dirt or finger marks on

the lens surfaces.

They will prevent you from clear observa-

tion of the specimen image.

oStrain-free glasses

The optical elements of this microscope

being constructed of strain-free glasses,

take particular caution in handling the

objectives and condenser lenses not to

cause strain to them.

4D> Focus knobs

Never attempt to adjust the tightness of

the right- and lefthand focus knobs by

turning the one, while holding the other in

th is model microscope, because of causi ng

disorder.

CARE AND MAINTENANCE CONTENTS

·············0

....... 0

·······8

NOMENCLATURE

II. ASSEMBLY.

III. PREPARATION

1. Centering the Lamp 8

2. Interpupillary Distance Adjustment 0

3. Diopter Adjustment 0

4. Optical Path Change-over in the

Trinocular Eyepiece Tube "TP" 0

5. Centering the Objectives 0

6. Centering the Condenser Lens 4@

IV. MICROSCOPY 41

1. Operating Procedure 41

2. Manipulation of Each Element ~

1) Focusing ~

2) Condenser aperture diaphragm ~

3) Field diaphragm ~

4) Circular graduated stage ~

5) Objectives 4J)

6) Eyepieces 4J)

7) Achromat strain-free condenser 4J)

8) Bertrand lens 4D

9) 1/4 A& tint plate 4D

10) Dia-polarizer and analyzer 4D

11) Filters G)

12) Lowering the substage G)

13) Illumination system G)

V. PHOTOMiCROGRAPHy 4D

VI. ACCESSORIES ~

1. Senarmont Compensator ~

2. Quartz Wedge ~

3. Monocular Eyepiece Tube "AP" W

4. Universal epi-illuminator W

5. Attachable Mechanical Stage Type "E". ~

6. Universal Stage ~

VII. TROUBLE SHOOTING TABLE ~

REFERENCE ~

ELECTRIC SPECIFICATIONS 0

8Cleaning the painted surfaces

Avoid the use of any organic solvent (for

example, thinner, ether, alcohol, xylene

etc.) for cleaning the painted surfaces and

plastic parts of the instrument.

oCleaning the lenses

To clean the lens surfaces, remove dust

using a soft brush or gauze. Only for

removing finger marks or grease, should

soft cotton cloth, lens tissue or gauze

lightly moistened with absolute alcohol

(ethanol or methanol) be used.

For cleaning the objectives and immersion

oil use only xylene.

Observe sufficient caution in handling

alcohol and xylene.

8Never attempt to dismantle!

Never attempt to dismantle the instrument

so as to avoid the possibility of impairing

the operational efficiency and accuracy.

oWhen not in use

When not in use, cover the instrument with

the accessory vinyl cover, and store it in a

place free from moisture and fungus.

It is especially recommended that the

objectives and eyepieces be kept in an

airtight container containing desiccant.

oPeriodical checking

To maintain the performance of the instru-

ment, we recommend to check the instru-

ment periodically. (For details of this

check, contact our agency.)

I.NOMENCLATURE

Interpupillary distance scale

Diopter ring

CF eyepiece

Eyeguard

Bertrand lens ring

Centering nosepiece

CF Achromat P objective

(Stra in-free)

Vernier

Circular graduated stage

Achromat strain-free condenser

Orientation plate

Field lens

Brightness control dial

(including power switch)

Microflex clamp screw

Trinocular eyepiece tube "TP"

Optical path change-over knob

Eyepiece tube clamp screw

Analyzer knob

-----------

IIntermediate tube clamp screw

111'1

Substage clamp screw

Socket sleeve

Lamp vertical

centering ring

Lamp lateral

centering screw

Lamp input plug

Filter receptacle

Filter frame

~ ND16&GIFI

(NCB10. ND2.

Dust capLamp centering tool

Fig. 1

Analyzer rotation ring

Analyzer clamp screw

Intermediate tube "P"

Nosepiece clamp screw

45° click stop lever

Condenser focus knob

Coarse focus knob

Fine focus knob

Lamp housing

clamp screw

Arm rest

-..

Compensator slot

1/4 A&tint plate

Nosepiece centering screw

Specimen clip

Condenser aperture

diaphragm control ring

Dia-polarizer

-------

Condenser clamp screw

Brightness indicator

Field diaphragm control ring

X-POL stand

Fig.2

II. ASSEMBLY

• To assemble the microscope, follow the procedure in the order given:

Substage

clamp scre,

Achromat strain-free condenser

Insert the condenser into the con-

denser carrier, facing the aperture

number plate toward the operator.

Fasten the clamp screw on the left

side of the carrier.

Circular graduated stage

Release the substage clamp screw using

a driver, and slide the substage on the

dovetail fitting. In such a position that

the top ends of the substage and the

dovetail are at the same level, fasten

the clamp screw.

CF eyepiece

Insert the eyepiece CFW 10XCM

into the right-hand sleeve, fitting

the pin of the eyepiece in the

right-hand notch of the sleeve.

Into the left-hand sleeve, insert

theCFW10X.

1/4 A&tint plate

Remove the screw by the side of

the 1/4 "A. plate of the 1/4 "A. & tint

plate and insert it into the com-

pensator slot of the intermediate

tube "P", facing the positioning

groove toward the operator side.

Reattach the removed screw.

Specimen clip

Place the clip on the stage

using holes on the stage sur-

face.

Aperture

number

plate

@Centering nosepiece

17 Lower the stage sufficiently.

Releasing the nosepiece clamp

screw on the left side of the

microscope arm, insert the nose-

piece. Making sure of positive

fitting of the pin on the micro-

scope arm into the nosepiece

groove, refasten the clamp screw.

@6CF Achromat P objective (Strain-free)

Mount the objectives on the nose-

piece in such positions that their

magnifying power increases as the

nosepiece is revolved clockwise.

lowest power

Leveling foot screw

For stable installation of the

microscope, manipulate the

adjusting screw at one foot on

the bottom of the microscope

base.

Power source voltage

Set the input voltage to the power

source voltage by means of the

change-over switch on the bottom

of the base.

If the illumination is found too

bright or unstable, when the switch is

turned ON, make adjustment of the

lowest voltage in the following way:

1) Turn the brightness control dial

to OFF.

2) Turn the lowest voltage adjusting

screw on the bottom of the

microscope base counterclockwise

to the limit, using a screw driver.

3) Turn the brightness control dial

to ON_ At this time, the lamp

voltage will be highest, immedi-

ately after lighting.

4) In this condition, gently turn the

lowest voltage adjustingsc;:ew

clockwise, to set the voltage to

nearly 4 on the ind icator.

Bottom of the Base

~-------

Bottom of the Base

Eyepiece tube

Attach the eyepiece tube on the intermediate tube "P", fitting

the notch of the circular dovetail on the end of the clamp screw.

Fasten the clamp screw.

Intermed iate tube "P"

Attach the intermediate tube "P" on the arm of the X-POL stand,

fitting the notch of the circular dovetail on the end of the

clamp screw. Fasten the clamp screw.

Lamp input plug

Connect the plug to the

receptacle on the filter

receptacle's left side.

®Lamp housing

Insert the lamp

housing into the col-

lector lens, and fas-

ten the clamp screw

on the left side of

the housing.

@Filter

Place the filter into the filter

frame. After centering the lamp,

put the filter with the frame into

the filter receptacle. No frame is

provided for the diffuser.

®Power input plug

Connect the plug to the re-

ceptacle on the fi Iter recep-

tacle's right side.

Insert the lamp bulb with

its pins into the accepting

holes in the socket.

Note: Don't touch the

bulb surface directly

with the fi nger.

f3\ Halogen lamp bulb~ (12V-50W)

Filter frame

Eyepiece tube clamp screw

Socket sleeve

Insert the socket sleeve into

the lamp housing, and fasten

it firmly with the clamp screw.

Socket sleeve clamp screw

Fig. 3

III. PREPARATION

Lamp housing

clamp screw

Filament

image

Condenser

aperture

diaphragm

Socket sleeve

clamp screw

Lamp vertical

centering ring

Lamp lateral

centering screw

Fig. 6

7) Release the socket sleeve clamp screw (Fig.

6). Turning the lamp lateral centering screw

and vertical centering ring, bring the fila-

ment image to the center, as shown in

Fig.7.

Fig. 7

8) As shown in Fig. 8, put the diffuser, with

its matte surface faced toward the micro-

scope stand, into the filter receptacle

which is the closest to the microscope

stand.

Fig. 8

The above centering procedure should be car-

ried out, when replacing the lamp bulb.

Lamp housing

Lamp

centering

tool

1. Centering the Lamp

ND filter

1) Connect the power source cord to the

socket.

2) Turn the brightness control dial to switch

ON and adjust the voltage to 6 on the

indicator.

3) Place the specimen on the stage, and focus

on the specimen using lOx objective. In

this case, open the condenser aperture and

field diaphragms to the largest extent.

4) Roughly center the condenser lens using

lOX objective, following the procedures

given on P. 10- 6.

5) Put the lamp centering tool on the field

lens and onto the tool place a NO filter.

(Fig. 4)

Fig. 4

6) Stop down the condenser aperture dia-

phragm, release the lamp housing clamp

screw, and move the lamp housing back

and forth (Fig. 5), until a sharp image of

the lamp filament appears on the aperture

diaphragm surface, which can be seen by

the reflection from the NO filter.

Fig.5

Fig. 13

Vertical photo

tube: 86%

Observation Observation

tube: 100% tube: 14%

+-- --+

~OPtical path

change-over knob

Fig. 12

Fig.11

*Since the CF eyepieces are of high eye-

point type, it is not necessary for the user

putting on his spectacles to remove them.

Only fold down the eyeguard rubber.

(Fig.13)

4. Optical Path Change-over in the

Trinocular Eyepiece Tube "TP"

(Fig. 11)

5. Centeringthe Objectives

1) Place the specimen on the stage, and focus

on the specimen. Bring an appropriate tar-

get to the center of the cross lines in the

eyepiece.

2) Insert the centering tools in the centering

screws on the nosepiece. (F ig. 14)

2. Interpupillary DistanceAdjustment I

Place a specimen on the stage, and focus on the

specimen.

As shown in Fig. 9, adjust the interpupillary

distance, so that both the right and left view-

fields become one. '

Fig. 9

Fig. 10

3. Diopter Adjustment

Rotate the diopter ring on the eyepiece CFW

lOx CM until the cross lines are seen clear.

(Fig. 10)

(For b inocu lar observation)

1) Mount the specimen on the stage. Swing

the objective 10 x into position, and bring

the specimen image into focus looking into

the right-hand eyepiece.

2) Witll.Out manipulating the coarse-and-fine

focus knob, turn the diopter ring on the

left-hand eyepiece to focus on the speci-

men.

Fig. 14

3) Rotate the stage about 1800, and the target

is displaced from the center of the cross

lines. Move the objective using the center-

ing tools so that the center of the cross

Iines comes to one half position of the

displacement of the target. (F ig. 15)

Rotate

1800

Target-

A half

position

of the

displacement

Image of field

[~:a~~~~~~

~'\

)

,,>J

":/

Eyepiece

viewfield stop

Fig. 16

Fig. 15

• Repeat the above procedure two or three

times, and the rotation center of the stage

coincides with the cross Iines center .

• Carry out centering for each objective.

6. Centering the Condenser Lens

1) Close the field diaphragm in the micro-

scope base to its smallest size by means of

the field diaphragm control ring. Rotate

the condenser focus knob to move the

condenser vertically so that a sharp image

of the field diaphragm is formed on the

specimen surface.

2) Bring the field diaphragm image to the

center of the field of view by means of

the condenser centering screws.

(Fig. 16-1IJ)

3) Change over to the objective 40 x, and

adjust the field diaphragm so that the

image of the diaphragm is about the same

as the eyepiece viewfield stop, as shown in

Fig. 16-~. If not centered, use the con-

denser centering screws again.

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