Nikon Eclipse TS100 Bedienungsanleitung

培養倒立顕微鏡

ECLIPSE

TS100/TS100-F

TS100LED MV/TS100LED-F MV

使用説明書

Inverted Microscope

ECLIPSE

TS100/TS100-F

TS100LED MV/TS100LED-F MV

Instructions

M590J/E 11.10.NC.1 (1/2)

E-1

Introduction

Thank you for purchasing a Nikon product.

This instruction manual is written for users of the Nikon Inverted Microscope ECLIPSE

TS100/TS100-F/TS100LED MV/TS100LED-F MV.

To ensure correct usage, read this manual carefully before operating the product.

•No part of this manual may be reproduced or transmitted in any form without prior

written permission from Nikon.

•The contents of this manual are subject to change without notice.

•The equipment described in this manual may differ from the actual product in its

appearance.

•Although every effort has been made to ensure the accuracy of this manual, errors or

inconsistencies may remain. If you note any points that are unclear or incorrect, please

contact your nearest Nikon representative.

•Some of the equipment described in this manual may not be included in the set you have

purchased.

•If you intend to use any other equipment with this product, read the manual for that

equipment too.

•If the equipment is used in a manner not specified by the manufacturer, the protection

provided by the equipment may be impaired.

Training

You can use this product without the need of special training sessions by reading this manual thoroughly before use.

Please contact your nearest Nikon representative if you have any questions or find any errors and anything you are

aware of.

Contents

E-2

Contents

Introduction ......................................................................................................E-1

Safety Precautions .............................................................................................E-3

WARNING and CAUTION Symbols Used in This Manual ..........................E-3

Meaning of Symbols Used on the Product.............................................E-3

WARNING and CAUTION for Using the TS100/TS100-F ................E-4

WARNING and CAUTION for Using

the TS100LED MV/TS100LED-F MV .................................................E-8

Notes on Handling the Product........................................................................... E-10

1Names of Parts.......................................................................................... E-12

2Names of Operational Parts......................................................................... E-13

3Microscopy ............................................................................................... E-14

1 Microscopy................................................................................. E-14

1-1 Phase Contrast Microscopy ....................................................... E-17

1-2 Modulation Contrast (NAMC) Microscopy .................................... E-19

1-3 Bright-field Microscopy............................................................. E-22

4Operation of Each Part ............................................................................... E-23

1 Aperture Diaphragm ................................................................... E-23

2 Specimen and Stage ................................................................... E-24

3 Focusing Device.......................................................................... E-25

4 Filters ....................................................................................... E-26

5 Objectives ................................................................................. E-26

6 Modulation Contrast Method......................................................... E-29

7 Photomicrography (for TS100-F/TS100LED-F MV only).................... E-31

5Assembly.................................................................................................. E-33

1 Assembling the Basic Set............................................................. E-33

2 Assembling Components Not Included in the Basic Set.................... E-36

6Troubleshooting......................................................................................... E-38

1 Optical ...................................................................................... E-38

2 Operational................................................................................ E-42

3 Electrical ................................................................................... E-44

7Care and Maintenance................................................................................ E-46

1 Cleaning the Lenses.................................................................... E-46

2 Cleaning the Microscope .............................................................. E-46

3 Disinfecting the Microscope.......................................................... E-46

4 Storing the Microscope................................................................ E-46

5 Regular Inspection (Charged)....................................................... E-46

8Specifications............................................................................................ E-47

1 Microscopy (Principles)................................................................ E-47

2 Performance Properties ............................................................... E-47

3 Physical Properties...................................................................... E-50

E-3

Safety Precautions

To ensure correct and safe operation, read this manual before using the product.

WARNING and CAUTION Symbols Used in This Manual

Although this product is designed and manufactured to be completely safe during use, incorrect usage or failure to

follow the safety instructions provided may cause personal injury or property damage. To ensure correct usage, read

this manual carefully before using the product. Do not discard this manual and keep it handy for easy reference.

Safety instructions in this manual are marked with the following symbols to highlight their importance. For your safety,

always follow the instructions marked with these symbols.

Symbol Description

WARNING Disregarding instructions marked with this symbol may lead to serious

injury or death.

CAUTION Disregarding instructions marked with this symbol may lead to injury

or property damage.

Meaning of Symbols Used on the Product

The symbol appearing on the product indicates the need for caution at all times during use.

Always refer to the instruction manual and read the relevant instructions before manipulating any part to which the

symbol has been affixed.

Symbol Description

CAUTION! Biohazard

This symbol label attached on the stages reminds you of the following:

•WARNING: Spillage of a sample from a vessel onto the microscope, presents a biohazard risk.

•To avoid biohazard contamination, do not touch the contaminated portion with your bare

hands.

•Decontaminate the contaminated portion according to the standard procedure of your

laboratory.

CAUTION for heat (for TS100/TS100-F only)

This symbol label attached on the lamphouse cover reminds you of the following:

•Lamp and its surrounding areas (including the lamphouse cover) become very hot during and

immediately after illumination.

•Risk of burns. Do not touch the lamp and its surrounding areas during and immediately after

illumination.

•When replacing the lamp, wait approximately 30 minutes after turning off the lamp, and

make sure that the lamp and its surrounding areas have cooled sufficiently before working.

CAUTION (for TS100/TS100-F only)

This symbol label attached near the AC inlet reminds you of the following:

•Check the input voltage before turning on the microscope.

(The input voltage is given on the "nameplate" and on immediate right of the AC inlet.)

•The voltage selector on the microscope sent to the United States is set to “120V” at the

factory.

•If the input voltage shown differs from the local voltage level, do not turn on the microscope.

Do the following instead:

Different voltage on the nameplate: Contact your nearest Nikon representative.

Different voltage on right of the AC inlet: Change the input voltage setting; refer to P.33.

Safety Precautions

E-4

WARNING and CAUTION for Using the TS100/TS100-F

1. Do not disassemble.

Disassembly may cause malfunction and/or electrical shock, and will lead to the forfeiture of all claims

against warranty. Do not disassemble any part other than those described in this manual. If you

experience any problem with the microscope, contact your nearest Nikon representative.

2. Read the instructions thoroughly.

To ensure safety, thoroughly read this manual and the manuals for other equipment to be used with

this product. In particular, be sure to follow the warnings and cautions at the beginning of the manuals.

3. Input voltage

Make sure that the input voltage indicated on two places (on the nameplate and on the immediate right

of the AC inlet at the microscope's rear) are the same as your local voltage. Do not use the microscope

if the local voltage differs from that required by the microscope. Follow the instructions below if this is

the case. Use of the microscope under the incorrect voltage may cause over-heating due to

over-current, possibly resulting in a fire and damaging the microscope.

•If the voltage indicated on the nameplate differs from local voltage, do not turn on the power but

contact your nearest Nikon representative immediately.

•If the voltage indicated to the immediate right of the AC inlet differs from local voltage, change the

input voltage setting as described on P.33 before turning on the power. The following values may be

set for the input voltage.

•If the nameplate reads “100/110/120 V ~”, the input voltage may be set to 100, 110 or 120 V

AC. (Factory setting: 120 V AC)

•If the nameplate reads “220/230/240 V ~”, the input voltage may be set to 220, 230 or 240 V

AC. (Factory setting: 240 V AC)

4. Use only the specified lamp and fuse.

Use only the specified lamp and fuse. Use of other lamp and fuse may result in damage to the

microscope or fire.

•Specified lamp: Halogen lamp 6 V-30 W (PHILIPS 5761)

•Specified fuse: 250V, 1A, time-lag, low-breaking type 5x20 miniature fuse x2

Input voltage indications

WARNING

Safety Precautions

E-5

5. Cautions on lamp replacement

•When replacing the lamp, wait approximately 30 minutes after turning off the lamp, and make sure

that the lamp and its surrounding areas have cooled sufficiently before working.

•To prevent electric shock and product damage, turn off the power of the microscope (press the

power switch to the “○” position) and unplug the power cord from the wall outlet before replacing

the lamp.

•When replacing the lamp, be sure that the lamp socket is not damaged. If it is damaged, it may

cause lamp failure or overheating. In addition, install the lamp into the lamp socket until it hits the

limit. If the lamp is not properly installed, it may fall off or cause overheating or smoke due to

mal-connection.

•After replacing the lamp, be sure to attach the lamphouse cover. Never use the product without the

lamphouse cover attached.

•Do not break the used lamp. It should be disposed of as an industrial waste, according to the local

regulations and rules.

6. Cautions on the power cord

•If the nameplate reads “100/110/120 V ~”:

Be sure to use the provided power cord. Use of other power cords may result in malfunction or fire.

Do not use the provided power cord for other purpose.

See Chapter 8, “Specifications - 2 Performance Properties” for the provided power cord.

•If the nameplate reads “220/230/240 V ~”:

Be sure to use the specified power cord. Use of other power cords may result in malfunction or fire.

See Chapter 8, “Specifications - 2 Performance Properties” for the specified power cord.

7. Heat from the light source

Do not touch the lamphouse while the lamp is on and for about 30 minutes after it is turned off as it

becomes extremely hot.

•Allow the lamp to cool sufficiently (for at least 30 minutes after being turned off) before replacing

the lamp.

•To avoid burns, never touch the lamp and its surrounding areas while the lamp is on and for about

30 minutes after the lamp has been turned off.

•To avoid the risk of fire, do not allow cloth, paper, or highly flammable volatile materials, such as

gasoline, petroleum benzine, acetone, thinner or alcohol, to come near the lamp and its surrounding

areas while the lamp is on and for about 30 minutes after the lamp has been turned off.

WARNING

Safety Precautions

E-6

8. Notes on handling flammable solvents

The following flammable solvents are used with the product:

•Immersion oil (Nikon immersion oil for oil immersion objectives)

•Absolute alcohol (ethyl alcohol or methyl alcohol for cleaning optical parts)

•Petroleum benzine (for wiping off the immersion oil)

•Medical alcohol (for disinfecting the microscope)

Never hold a flame near these solvents. When using a solvent, thoroughly read the instructions

provided by the manufacturer, and handle correctly and safely. Note the following precautions when

using solvents with the product.

•Keep solvents away from the lamp, lamphouse cover and any other parts that may become hot.

•Keep solvents away from the product and its surroundings when turning on/off the power switch or

plugging/unplugging the power cord.

•Be careful not to spill the solvents.

9. Hazardous sample

This microscope should mainly be used for microscopic observation and micromanipulation of living

cells and tissue cultures in Petri dishes, microtiter plates, etc.

When handling a sample, check to determine whether the sample is hazardous.

Handle hazardous samples according to the standard procedure of your laboratory. If the sample is of

an infectious nature, wear rubber gloves to avoid infection, and be careful not to spill the sample. In

the event of spillage of a sample from a vessel onto the microscope, decontaminate the contaminated

portion according to the standard procedure of your laboratory.

WARNING

Safety Precautions

E-7

1. Turn off the power when assembling the microscope, replacing lamps and fuses, and

plugging or unplugging the power cord.

To prevent electrical shock and/or fire, always turn off the power of the microscope (press the power

switch to the “○” position) before plugging or unplugging the power cord. Also, always turn off the

power of the microscope (press the power switch to the “○” position) and unplug the power cord when

assembling the microscope, or replacing lamps or fuses.

2. Do not wet the microscope or allow foreign matter to get inside.

If the microscope becomes wet, a short circuit may result that may damage it or make it extremely

hot. Similarly, a short may occur if foreign matter gets inside. If you accidentally spill a liquid on the

microscope, immediately turn off the power of the microscope (press the power switch to the “○”

position) and unplug the power cord. (Take care not to touch the cord with wet hands at this time.)

After unplugging the microscope, use a dry cloth to wipe away any moisture.

If liquid spills on the water tray, use tissue paper to absorb it from the groove on the left. Then remove

the water tray cover using a hexagonal wrench. Wipe dry the inside if there is any moisture. Check if

the lamp cable connector or AC inlet on the rear panel are wet, and if so, wipe them dry.

If any liquid or foreign matter gets inside, do not use the microscope, but contact your nearest Nikon

representative.

3. Cautions on carrying the microscope

•Securely hold the microscope at the bottom front and back

when transporting it.

•Do not grasp the focus knobs, eyepiece tube, stage, etc.

when carrying the microscope. This may cause the part to

come off, resulting in damage to the microscope.

4. Cautions on assembling the product

•Take care to avoid pinching your fingers and hands.

•Scratches and dirt (i.e. fingerprints) on optical components

such as lenses and filters will degrade the microscope image.

When assembling the product, be careful not to scratch or

directly touch the optical components.

5. Disposal of the microscope

To avoid biohazard risk, dispose of the microscope as the

contaminated equipment according to the standard procedure

of your laboratory.

CAUTION

Safety Precautions

E-8

WARNING and CAUTION for Using

the TS100LED MV/TS100LED-F MV

1. Do not disassemble.

Disassembly may cause malfunction and/or electrical shock, and will lead to the forfeiture of all claims

against warranty. Do not disassemble any part other than those described in this manual. If you

experience any problem with the microscope, contact your nearest Nikon representative.

2. Read the instructions thoroughly.

To ensure safety, thoroughly read this manual and the manuals for other equipment to be used with

this product. In particular, be sure to follow the warnings and cautions at the beginning of the manuals.

3. Input voltage

This product can be used with 100 to 240 V AC at 50/60 Hz, and can be used with most wall outlets in

the world. However, note that you avoid using the product under an environment where the supply

voltage may extremely fluctuate.

4. Cautions on the power cord

Be sure to use the provided (or specified) power cord. Use of other power cords may result in

malfunction or fire.

•See Chapter 8, “Specifications - 2 Performance Properties” for the specified power cord.

•To prevent electric shock, turn off the power switch (press the power switch to the “○” position)

before connecting or disconnecting the power cord.

•Note that this product is classified as Class I for electric shock protection. Be sure to connect it to a

protective earth terminal.

5. Notes on handling flammable solvents

The following flammable solvents are used with the product:

•Immersion oil (Nikon immersion oil for oil immersion objectives)

•Absolute alcohol (ethyl alcohol or methyl alcohol for cleaning optical parts)

•Petroleum benzine (for wiping off the immersion oil)

•Medical alcohol (for disinfecting the microscope)

Never hold a flame near these solvents. When using a solvent, thoroughly read the instructions

provided by the manufacturer, and handle correctly and safely. Note the following precautions when

using solvents with the product.

•Keep solvents away from any parts that may become hot.

•Keep solvents away from the product and its surroundings when turning on/off the power switch or

plugging/unplugging the power cord.

•Be careful not to spill the solvents.

WARNING

Safety Precautions

E-9

6. Hazardous sample

This microscope should mainly be used for microscopic observation and micromanipulation of living

cells and tissue cultures in Petri dishes, microtiter plates, etc.

When handling a sample, check to determine whether the sample is hazardous.

Handle hazardous samples according to the standard procedure of your laboratory. If the sample is of

an infectious nature, wear rubber gloves to avoid infection, and be careful not to spill the sample. In

the event of spillage of a sample from a vessel onto the microscope, decontaminate the contaminated

portion according to the standard procedure of your laboratory.

1. Turn off the power when assembling the microscope and plugging or unplugging the

power cord.

To prevent electrical shock and/or fire, always turn off the power of the microscope (press the power

switch to the “○” position) before plugging or unplugging the power cord. Also, always turn off the

power of the microscope (press the power switch to the “○” position) and unplug the power cord when

assembling the microscope.