Nikon Eclipse Ci-S Bedienungsanleitung

Turn on the power.

Press the switch to the "|" position to turn on

the power to the microscope (the power LED

on the front of the main body will light up to

indicate that dia-illumination is turned ON).

M568 E

11.8.NF.1 (2/2)

Bright-field Microscopy Quick Guide

Lower the condenser slightly from the uppermost position.

Turn the condenser focus knob until the condenser is positioned at

the upper limit (where it clicks to a stop), and then lower it a little.

(2) Rotate the stage knob to move the stage and bring the target into

the optical path.

(So that the sample sealed under the cover glass will be lighted.)

Fully open the field diaphragm and aperture diaphragm.

Turn the field diaphragm dial and the aperture diaphragm lever

clockwise to open them completely.

Bring the 10x objective into the optical path.

Turn the nosepiece to bring the 10x objective into the optical path.

Turn the nosepiece until it clicks.

Place a specimen on the stage, and move the stage

to bring the target into view.

(1) Open the claw of the specimen holder's moving part and place

the specimen onto the stage, gently stowing the claw back

to fix the specimen.

Focus on the specimen.

(See Chapter 2, Section 2 in the instruction manual for details)

(1) When using the trinocular tube or ergonomic tube, push in

the optical path switching lever to distribute 100% light to

the binocular section.

(2)

(3)

Look into the eyepiece and turn the coarse focus knob away

to raise the stage to the upper limit. From there, focus on

the specimen by lowering the stage.

When the focus was roughly adjusted using coarse focus knob,

turn the fine focus knob to accurately adjust the focus.

Avoid the following actions, which can

cause equipment malfunction.

Notes on controlling the focus knobs

(4) Adjust the brightness of the field of view by turning

the dia-illumination lamp brightness control knob.

Rotating the right and left focus

knobs in opposite directions.

Turning the coarse focus knob past

its limit point.

CLAMP

TORQUE

ND4 ND8

OUT

ND4 ND8

OUT

CLAMP

POWER

0.8

0.6

0.4

0.2

POWER

0.8

0.6

0.4

0.2

POWER

0.8

0.6

0.4

0.2

TORQUE

CLAMP

WER

CLAMP

TORQUE

ND4 ND8

OUT

CLAMP

TORQUE

ND4 ND8

OUT

Adjust the diopter.

(See Chapter 2, Section 4 in the instruction manual for details)

(1)

(2)

(3)

(4)

Turn the diopter adjustment ring on the right and

left eyepieces to align the end face of the diopter

adjustment ring with the line.

(This is the diopter adjustment reference position.)

Focus on the specimen using the 40x objective.

Bring the 10x (or 4x) objective into the optical path.

Look into the right eyepiece with your right eye and the left

eyepiece with your left eye. Turn the diopter adjustment ring

of each eyepiece to focus on the specimen.

At this point no focus knobs are used.

Line

(5) Repeat steps (2) through (4) to make sure the focus has been

adjusted properly.

For easy adjustment, look into

the eyepiece as if you were looking

at a distant object.

Focus and center the condenser.

(See Chapter 2, Section 5 in the instruction manual for details)

(1) Look into the eyepiece with the field diaphragm stopped down

to the minimum. Focus on the field diaphragm image using

the condenser focus knob, then adjust the condenser centering

screws to center the diaphragm image within the field of view.

(2)

(3)

Bring the 40x objective into the optical path to check the focus

and centering of the field diaphragm image. Make adjustments

in the same way as step (1) as necessary.

Turn the field diaphragm dial and adjust the field diaphragm image

so that its size is almost the same as the field of view.

Adjust the interpupillary distance.

Look into both eyepieces and rotate the binocular part to adjust

the binocular part's opening until the fields of view for the right

and left eyes coincide.

Be sure to adjust the aperture

diaphragm each time you change

the objective.

(You can see the aperture diaphragm

image with the centering telescope.)

Opening the field diaphragm too much

results in stray light entering the field

of view, generating flare and reducing

the image contrast.

Be sure to adjust the field diaphragm

each time you change the objective.

Adjust the field diaphragm

Turn the field diaphragm dial to adjust the field diaphragm so that

it almost circumscribes the field of view.

View the specimen.

Rotate the stage knob to move the target. If the target is not in focus,

use the focus knob to adjust the focus.

Turn off the power switch (press to

the " " position) for the microscope.

(The power LED on the front of

the main body will turn off.)

Turn off the power.

Select the desired objective.

Turn the nosepiece to bring the desired objective into the optical path.

Adjust the aperture diaphragm.

(See Chapter 2, Section 6 in the instruction manual for details)

Turn the aperture diaphragm lever on the condenser to adjust

the aperture diaphragm so that it is set to 70 to 80% of

the numerical aperture of the objective used.

Focus on the specimen.

POWER

0.8

0.6

0.4

0.2

Field diaphragm

image

Eyepiece field

of view

Field diaphragm

image

Eyepiece field

of view

CLAMP

Pupil of

objective

Appropriate size of

the aperture diaphragm

70 to 80

100

Field of view

Circumscribe around

the field of view

OFF

ND4 ND8

OUT

CLAMP

TORQUE

ND4 ND8

OUT

Aperture diaphragm

image

Field diaphragm

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