Nikon LABOPHOT-POL Bedienungsanleitung

Nikon

Polarizing Microscope

LABOPHOT -POL

INSTRUCTIONS

NIPPON KOGAKU K.K.

CAUTIONS

oAvoid sharp knocks l

Handle the microscope gently, taking care

to avoid sharp knocks.

_When carrying the microscope

When carrying the microscope, hold its arm

with one hand, supporting the bottom of

the microscope base with the other. The

instrument weighs about 8 kg.

8Place for using

Avoid the use of the microscope in a dusty

place, where it is subject to vibrations or

exposed to high temperatures, moisture or

direct sunlight.

oPower source voltage

..,...ForEuropean districts only-

Make sure of the power source voltage,

220V or 240V, by means of the input

voltage change-over switch wh ich is on the

bottom of the microscope base.

oExchanging the lamp bulb and fuse

Before replacing the lamp bulb (6V-20W)

or fuse, turn OF F the power switch and

disconnect the plug of the power source

cord.

In such cases as of replacement, do not

touch the lamp bulb with bare hands, im-

mediately after putting out the lamp.

(it Dirt on the lens

Do not leave dust, dirt or finger marks on

the lens surfaces.

They will prevent you from clear observa-

tion of the specimen image.

~Strain-free glasses

The optical elements of this microscope

being constructed of strain-free glasses,

ta ke particular caution in hand Iing the

objectives and condenser lenses not to

cause strain to them.

8Focus knobs

Never attempt to adjust the tightness of

the right- and lefthand focus knobs by

turning the one, while holding the other in

this model microscope, because of causing

disorder.

CARE AND MAINTENANCE

oCleaning the lenses

To clean the lens surfaces, remove dust

using a soft hair brush or gauze. Only for

removing finger marks or grease, should

soft cotton cloth, lens tissue or gauze

lightly moistened with absolute alcohol

(methanol or ethanol) be used.

For cleaning the objectives and immersion

oil use only xylene.

Observe sufficient caution in handling

alcohol and xylene.

f)Cleaning the painted surfaces

Avoid the use of any organic solvent (for

example, th inner, ether, alcohol, xylene

etc.) for cleaning the painted surfaces and

plastic parts of the instrument.

8Never attempt to dismantle!

Never attempt to dismantle the instrument

so as to avoid the possibility of impairing

the operational efficiency and accuracy.

OWhen not in use

When not in use, cover the instrument with

the accessory vinyl cover, and store it in a

place free from moisture and fungus.

It is especially recommended that the

objectives and eyepieces be kept in an

airtight container containing desiccant.

oPeriodical checking

To maintain the performance of the instru-

ment, we recommend to check the instru-

ments periodically. (For details of this

check, contact our agency.)

CONTENTS

I. NOMENCLATURE 4

II. ASSEMBLy 6

III. PREPARATION 8

1. Interpupillary Distance Adjustment 8

2. Diopter Adjustment 8

3. Optical Path Change-over in the

Trinocular Eyepiece Tube "TP" 8

4. Centering the Objectives 8

5. Centering the Condenser Lens 9

6. Orientation of the Dia-polarizer 9

IV. MICROSCOPY 10

1. Operating Procedure 10

2. Manipulation of Each Element 11

1) Focusing 11

2) Condenser aperture diaphragm 11

3) Field diaphragm 11

4) Circular graduated stage 11

5) Objectives 11

6) Eyepieces 12

7) Achromat strain-free condenser 12

8) Bertrand lens 12

9) 1/4 A&tint plate 13

10) Dia-polarizer and analyzer _ 13

11) Filters 14

12) Illumination system 14

V. PHOTOMiCROGRAPHy 15

VI. ACCESSORIES 17

1. 5enarmont Compensator 17

2. Quartz Wedge 17

3. Monocular Eyepiece Tube "AP" 18

4. Epi-illuminator "M" 18

5. Attachable Mechanical Stage Type "E". 19

VII. TROUBLE SHOOTING TABLE 20

REFERENCE 23

ELECTRIC SPECIFICATIONS 23

I.NOMENCLATURE

Binocular eyepiece tube "BP"

Interpupillary distance scale

Diopter ring

CF eyepiece

Eyeguard

Bertrand lens flip-in/out ring

Centering nosepiece

CF Achromat P objective

(strain-tree)

Circular graduated stage

clamp screw

Vernier

Achromat strain-free condenser

Filters

(B& GIF)

Dust cap

Eyepiece tube clamp screw

Analyzer in/out knob

Intermediate tube

clamp screw

Coarse focus knob

Fine focus knob

Orientation plate

Filter receptacle

Fig. 1

Analyzer rotation ring

Intermediate tube "P"

Analyzer clamp screw

Condenser focus knob

Lamp socket

Field diaphragm

control ring

V-POL stand

Compensator slot

1/4 A&tint plate

Nosepiece centering screw

Specimen clip

Stage rotation clamp screw

Condenser centering screw

Condenser aperture

diaphragm control ring

Dia-polarizer

Condenser clamp screw

Field lens

Brightness control dial

(including power switch)

Fig.2

II. ASSEMBLY

1/4 A&tint plate

Remove the screw by the side of

the 1/4 Aplate of the 1/4 A& tint

plate and insert it into the com-

pensator slot of the intermediate

tube "P", facing the positioning

groove toward the operator side.

Reattach the removed screw.

®CF Achromat P objective

Mount the objectives on the nose-

piece in such positions that thfilir

magnifying power increasesasthe

nosepiece is revolved clockwise.

Input voltage change-over

switch

(For European districts only)

Make sure of the power source

voltage, 220V or 240V, by means

of the input voltage change-over

switch on the bottom of the

microscope base_

• To assemblethe microscope, follow the procedure in the order given:--'

CF eyepiece _

®Insert the eyepiece CFW 10XCM- '"

into the right-hand sleeve, fitting

the pin of the eyepiece in the

right-hand notch of the sleeve.

Into the left-hand sleeve, insert

the CFW lOx.

Bottom of the Base

@2 Achromat strain-free condenser

Insert the condenser into the con-

denser carrier, fad ng the aperture

number plate toward the operator.

Fasten the clamp screw on the left

side of the carrier.

Aperture number plate •.----

Specimen clip

Place the clip on the stage using

holes on the stagesurface.

Stage clamp screw

Circular graduated stage

Releasing the stage clamp screw

sufficiently, mount the stage on

the circular dovetail of the sub-

stage. Clamp the screw.

Dia-polarizer

After centering the objec-

tives and condenser, insert

the dia-polarizer into the

bottom of the condenser.

Leveling foot screw

For stable installation of the

microscope, manipulate the adjust-

ing screw at one foot on the

bottom of the microscope base.

•

---------. ?:=:

Y·POL stand

@T:.er

(]) Eyepiece tube

Attach the eyepiece tube on the intermediate tube "P", fitting

the notch of the circular dovetail on the end of the clamp screw.

Fasten the clamp screw.

Eyepiece tube clamp screw

®Intermediate tube "P"

Attach the intermediate tube "P" on the arm of the V-POL stand,

fitting the notch of the circular dovetai I on the end of the

clamp screw. Fasten the clamp screw.

Intermediate tube clamp screw

Halogen lamp bulb (6V-20W)

Insert the lamp bulb with its pins

into the accepting holes in the

socket.

Note Don't touch the bulb sur-

face directly with the fin-

ger.

Power source cord

t4'Lamp socket~ Insert the socket into the recep-

tacle on the rear of the base.

Place the filter on the field

lens. <p = 45

Fig. 3

III. PREPARATION

3.. Optical Path Change-over in the 1

Trinocular Eyepiece Tube "TP"

(Fig. 6)

Fig. 8

Vertical photo

tube: 86%

Observat ion

tube: 14%

Optical path

change-over knob

Fig. 7

Fig.6

'* Since the CF eyepieces are of high eye-

point type, it is not necessary for the user

putting on his spectacles to remove them.

Only fold down the eyeguard rubber.

(Fig.8)

I. 4. Centeringthe Objectives

1) Place the specimen on the stage, and focus

on the specimen. Bring an appropriate tar-

get to the center of the cross lines in the

eyepiece.

2) Insert the centering tools in the centering

screws on the nosepiece. (Fig. 9)

(For binocular observation)

1) Mount the specimen on the stage. Swing

the objective 10 x into position, and bring

the specimen image into focus looking into

the right-hand eyepiece.

2) Without manipulating the coarse-and-fine

focus knob, turn the diopter ring on the

left-hand eyepiece to focus on the speci-

men.

Fig. 5

____ J

Fig. 4

2. Diopter Adjustment

Rotate the diopter ring on the eyepiece CFW

10X CM until the cross lines are seen clear.

(Fig.5)

1. ·Interpupiflary DistanceAdjustment I

Place a specimen on the stage, and focus on the

specimen.

As shown in Fig. 4, adjust the interpupillary

distance, so that both the right and left view-

fields become one.

Fig.9

Fig. 10

• Repeat the above procedure two or three

times, and the rotation center of the stage

coincides with the cross lines center.

• Carry out centering for each objective.

Fig. 11

Eyepiece

viewfield stop

[.Im ..ageof field

diaphragm

iT \,

\ ....(')\.i \

\~~) )

rn~-/

Eyepiece

viewfield stop

6. Orientationof the Dii-pmarizer ·1

1) Place the specimen on the stage, focus on

the specimen using objective 40 X.

2) Set the analyzer scale on the intermediate

tube "P" to O.

3) Insert the dia-polarizer into the bottom

of the condenser asshown in Fig. 12.

A half

position

of the

displacement

Rotate

1800 .

Target

3) Rotate the stage about 1800, and the target

is displaced from the center of the cross

Iines. Move the objective using the center-

ing tools so that the center of the cross

Iines comes to one half position of the

displacement of the target. (Fig. 10)

Di'''OI''i''~~~

Fig. 12

4) Remove the eyepiece from the sleeve.

Observing the exit pupil of objective

inside, rotate the dia-polarizer so as to

form a dark cross image on the exit pupil

asshown in Fig. 13.

5. Centeringtbe CondenserLe~' ~I

1) Close the field diaphragm in the micro-

scope base to its smallest size by means of

the field diaphragm control ring. Rotate

the condenser focus knob to move the

condenser vertically so that a sharp image

of the field diaphragm is formed on the

specimen surface.

2) Bring the field diaphragm image to the

center of the field of view by means of

the condenser centering screws.

(Fig. 11-[I])

3) Change over to the objective 40 x, and

adjust the field diaphragm so that the

image of the diaphragm is about the same

as the eyepiece viewfield stop, asshown in

Fig. 11- ~. If not centered, use the con-

denser centering screws again.

Dark cross image

Fig. 13

IV. MICROSCOPY

A~> .... v: " >,,' ,<' ,:¥f:~i-0":'.?~~G+:t

..O~~atingProeed~ri'

f/Ji ~,:,fr%: ~

1) Turn the brightness control dial (including

power switch) to light the lamp.

2) Bring the analyzer and the Bertrand lens out of

the optical path. (Refer to P. 12 &13)

3) Place the specimen on the stage and swing the

10X objective into position. Focuson specimen.

4) Adjust the interpupillary distance and diopter.

(Refer to P.8)

5) Placethe filter on the field lens.

6) Carry out the centering procedure for the

objective. (Refer to P.8)

7) Carry out the centering procedure for the con-

denser. (Refer to P.9)

8) Bring the analyzer into the optical path.

9) Swing in the objective to be usedand refocus on

specimen.

10) Brightness is adjusted by changing the lamp

voltage.

Table 1

Orthoscopic

Conoscopic

microscopy microscopy

lOX or IN

Top lens of higher IN

condenser 4X or

OUT

lower

Bertrand lens

OUT

Circumscribed

lOX or

70% ~ 80% of the

the circumfer-

numerical aperture ence of the

Aperture higher

of the objective

conoscoplC

diaphragm 4X or field of view

Fully opened (or fullylower opened)

Circumscribed the

Circumscribed

lOx or circumference of

the circumfer-

Field higher

the eyepiece field

ence of the

diaphragm

of view

orthoscopic

4x or Fully opened

field of view

lower

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