Nikon ECLIPSE E200 MV Series Bedienungsanleitung

Microscope

ECLIPSE

E200 MV Series

E200LED MV Series

Instructions

M588E 11.10.NC.1 (1/2)

Introduction

Thank you for purchasing a Nikon product.

This instruction manual is written for users of the Nikon ECLIPSE E200 MV Series and E200LED MV

Series Microscopes.

To ensure correct usage, read this manual carefully before operating the product.

•No part of this manual may be reproduced or transmitted in any form without prior written permission from

Nikon.

•The contents of this manual are subject to change without notice.

•The equipment described in this manual may differ from the actual product in its appearance.

•Although every effort has been made to ensure the accuracy of this manual, errors or inconsistencies may

remain. If you note any points that are unclear or incorrect, please contact your nearest Nikon

representative.

•Some of the equipment described in this manual may not be included in the set you have purchased.

•If you intend to use any other equipment with this product, read the manual for that equipment too.

•If the equipment is used in a manner not specified by the manufacturer, the protection provided by the

equipment may be impaired.

Training

You can use this product without the need of special training sessions by reading this manual thoroughly

before use. Please contact your nearest Nikon representative if you have any questions or find any errors

and anything you are aware of.

Contents

Introduction ................................................................................................. i

Safety Precautions ..................................................................................... iv

WARNING and CAUTION Symbols Used in This Manual...............................iv

Symbols on the Product..........................................................................v

WARNING .......................................................................................vi

CAUTION ...................................................................................... viii

Notes on Handling the Product................................................................... ix

Chapter 1 Nomenclature of Each Part....................................................... 2

Chapter 2 Switches and Controls.............................................................. 4

Chapter 3 Microscopy Procedure (Quick Reference)................................. 6

Chapter 4 Microscopy Procedure (Detailed) ............................................. 8

Preparation Operations previous to the microscopy ...................................... 8

1 Turn on the power switch. ............................................................ 8

2 Lower the condenser slightly from its upper limit. ............................ 8

3 Fully open the field aperture and condenser aperture diaphragms.

(Field diaphragm control is only for microscopes

with the field diaphragm.) ............................................................ 9

4 Bring the 10x objective into the optical path. .................................. 9

5 Place the specimen slide on the stage and bring the specimen

into the optical path. ..................................................................10

6 Focus on the specimen. ..............................................................10

7 Adjust the diopter ring on the eyepieces........................................12

8 Adjust the interpupillary distance. ................................................13

9 Focus on the field diaphragm and center its position.

(This adjustment is only for microscopes with the field diaphragm.) ..14

Microscopy Operations for actual microscopy............................................15

10 Select the objective....................................................................15

11 Adjust the condenser aperture diaphragm. ....................................15

12 Focus on the specimen. ..............................................................17

13 Adjust the field diaphragm.

(This adjustment is only for microscopes with the field diaphragm.) ..18

14 Perform the microscopy. .............................................................18

15 Turn off the power switch............................................................21

Contents

iii

Chapter 5 Miscellaneous Operations....................................................... 22

1 Oil-Immersion Observation ..............................................................22

2 Torque Adjustment of the Coarse Focus Knob .....................................24

3 Upper Limit Bolt.............................................................................24

4 Eye Level Raiser.............................................................................25

5 Photomicrography and TV Microscopy ...............................................25

6 ND Filter for the Objective ...............................................................26

7 Cord Hangers ................................................................................27

Chapter 6 Assembly................................................................................ 28

1 Assembly of Standard Set ...............................................................28

2 Assembly of Additional Components..................................................30

3 Replacement of Consumable Materials...............................................32

Chapter 7 Optical Characteristics ........................................................... 34

1 Combinations of 10x (Field No. 20) Eyepiece with E-Plan Objectives ......34

2 Microscope Terminology ..................................................................34

Chapter 8 Troubleshooting ..................................................................... 36

1 Optical .........................................................................................36

2 Operational ...................................................................................41

3 Electrical.......................................................................................43

Chapter 9 Care and Maintenance ............................................................ 44

1 Cleaning the Lenses .......................................................................44

2 Cleaning the Microscope..................................................................44

3 Disinfecting the Microscope .............................................................44

4 Storing the Microscope....................................................................44

5 Periodical Inspections (Charged) ......................................................44

Chapter 10 Specifications......................................................................... 45

1 Microscopy (Principles) ...................................................................45

2 Performance Properties ...................................................................45

3 Physical Properties .........................................................................47

Safety Precautions

To ensure correct and safe operation, read this manual before using the product.

WARNING and CAUTION Symbols Used in This Manual

Although this product is designed and manufactured to be completely safe during use, incorrect usage or

failure to follow the safety instructions provided may cause personal injury or property damage. To ensure

correct usage, read this manual carefully before using the product. Do not discard this manual and keep it

handy for easy reference.

Safety instructions in this manual are marked with the following symbols to highlight their importance. For

your safety, always follow the instructions marked with these symbols.

Symbol Description

WARNING Disregarding instructions marked with this symbol may lead to serious injury or death.

CAUTION Disregarding instructions marked with this symbol may lead to injury or property damage.

Safety Precautions

Symbols on the Product

The symbols on the product indicate the need for caution at all times during use. Before operating a part

labeled with the following symbols, refer to the instruction manual and read the relevant instructions.

Symbol Meaning

Biohazard

This symbol can be found on the microscope body, and cautions the following:

•WARNING: The product may become biohazardous if a specimen is spilled onto the

product.

•To avoid exposure to biohazard, do not touch contaminated parts with your bare hands.

•Decontaminate the contaminated parts according to the standard procedures for your

facility.

CAUTION and CAUTION: HOT (for E200 MV series only)

This symbol can be found on the field lens unit (the lamp is set underneath the field lens unit),

and cautions the following:

•The lamp and its surrounding areas (including the field lens unit, field diaphragm centering

screws, and bottom of the microscope unit) will be extremely hot while and immediately

after using the lamp.

•To avoid the risk of burns, do not touch the lamp and its surrounding areas while or

immediately after using the lamp.

•When replacing the lamp, wait approximately 30 minutes after turning off the lamp, and

make sure that the lamp and its surrounding areas have cooled sufficiently before working.

•When replacing the lamp, see “WARNING: 5. Cautions on lamp replacement (for E200 MV

series only)” on P. vi to follow what is described there.

CAUTION: HOT (for E200 MV series only)

This symbol can be found when the field lens unit is removed from the microscope body, and

cautions the following:

•To avoid the risk of burns, do not touch the lamp and its surrounding areas immediately

after using the lamp.

•When replacing the lamp, wait approximately 30 minutes after turning off the lamp, and

make sure that the lamp and its surrounding areas have cooled sufficiently before working.

•When replacing the lamp, see “WARNING: 5. Cautions on lamp replacement (for E200 MV

series only)” on P. vi to follow what is described there.

CAUTION (for E200LED MV series only)

This symbol can be found on the field lens unit (the LED is set underneath the field lens unit),

and cautions the following:

•Make sure that the field lens unit is securely attached.

•Illuminating the LED with the field lens unit removed, a light of high intensity gets in your

eyes and causes an irritating discomfort in your eyes, resulting in a harmful effect on the

microscopy for a while.

•A light leak may lead to inability of observation when the filed lens unit is left removed.

Safety Precautions

WARNING

1. Do not disassemble.

Disassembling this product may result in electric shock or malfunction. Malfunctions and damage

due to such mishandlings will not be warranted. Do not disassemble any part that is not indicated

in this manual. If you experience problems with the product, contact your nearest Nikon

representative.

2. Read the instructions thoroughly.

To ensure safety, thoroughly read this manual and the manuals for other equipment to be used

with this product. In particular, be sure to follow the warnings and cautions at the beginning of the

manuals.

3. Input voltage

This product can be used with 100 to 240 V AC at 50/60 Hz, and can be used with most wall

outlets in the world. However, note that you avoid using the product under an environment where

the supply voltage may extremely fluctuate.

4. Use the specified lamp. (for E200 MV series only)

Use the specified lamp. Using an incorrect lamp may damage the product or cause a fire.

•Specified lamp

Halogen lamp 6 V-30 W (PHILIPS 5761)

5. Cautions on lamp replacement (for E200 MV series only)

•When replacing the lamp, wait approximately 30 minutes after turning off the lamp, and make

sure that the lamp and the field lens unit have cooled sufficiently.

•To prevent electric shock and product damage, turn off the power switch (press to the “{”

position) and unplug the power cord from the wall outlet before replacing the lamp.

•When replacing the lamp, be sure that the lamp socket is not damaged. If it is damaged, it may

cause lamp failure or overheating. In addition, install the lamp into the lamp socket until it hits

the limit. If the lamp is not properly installed, it may fall off or cause overheating or smoke due

to mal-connection.

•After replacing the lamp, be sure to attach the field lens unit. Never use the product without the

field lens unit attached.

•Do not break the used lamp. It should be disposed of as an industrial waste, according to the

local regulations and rules.

6. Cautions on the power cord

Be sure to use provided (or specified) power cord. Use of other power cords may result in

malfunction or fire.

•See Chapter 10, “Specifications” for the specified power cord.

•To prevent electric shock, turn off the power switch (press to the “{” position) before

connecting or disconnecting the power cord.

•Note that this product is classified as Class Ifor electric shock protection. Be sure to connect it

to a protective earth terminal.

Safety Precautions

vii

WARNING

7. Cautions on heat from the light source (for E200 MV series only)

The lamp becomes hot during use. Do not remove the field lens unit while the lamp is on, and be

sure the lamp has been off for 30 minutes before touching it.

•When replacing the lamp, make sure that the lamp is cool enough to touch (the light should be

off at least 30 minutes).

•Do not touch the lamp while it is on or until the lamp has been off for 30 minutes, as doing so

may result in burns.

•Never bring cloth, paper or flammable volatile substances such as gasoline, petroleum

benzine, acetone, thinner, or alcohol near a hot lamp, as a fire may result.

•Do not block the air vents on the field lens unit. If the field lens unit is covered or items are

placed on the field lens unit, heat dissipation may be hindered, causing the field lens unit to

become abnormally hot.

8. Notes on handling flammable solvents

The following flammable solvents are used with the product:

•Immersion oil (Nikon Immersion Oil for oil immersion objectives)

•Absolute alcohol (ethyl alcohol or methyl alcohol for cleaning optical parts)

•Petroleum benzine (for wiping off the immersion oil)

•Medical alcohol (for disinfecting the microscope)

Never hold a flame near these solvents. When using a solvent, thoroughly read the instructions

provided by the manufacturer, and handle correctly and safely. Note the following precautions

when using solvents with the product.

•Keep solvents away from the lamp, the field lens unit, and any other parts that may become

hot.

•Keep solvents away from the product and its surroundings when turning on/off the power

switch or plugging/unplugging the power cord.

•Be careful not to spill the solvents.

9. Notes on handling hazardous specimens

This microscope is mainly for use in microscopic observation of cells and tissue fixed on the slide.

When handling a sample, check to determine whether the sample is hazardous.

Handle hazardous samples according to the standard procedure for your laboratory.

If the sample is of an infectious nature, wear rubber gloves to avoid infection, and be careful not to

touch a sample. In the event of contact of a sample to the microscope, decontaminate the

contaminated portion according to the standard procedure for your laboratory.

Safety Precautions

viii

CAUTION

1. Turn off the power switch before assembling the microscope, replacing the lamp (for

E200 MV series only), and plugging in or unplugging the power cord.

Turn off the power switch before you plug or unplug the power cord to prevent electrical shock or

fire. Also turn off the power switch and then unplug the power cord before assembling the

microscope, and before changing the lamp. To turn off the power, press the power switch to the

“{” position.

2. Do not wet the product or allow intrusion of foreign matters.

Do not wet or spill liquids onto the product, as it may result in malfunction, overheat, or electric

shock. If water or other liquids are accidentally spilled onto the product, immediately turn off the

power switch (press to the “{” position) and unplug the power cord from the wall outlet. Then,

wipe off the liquid with a piece of dry cloth. Intrusion of foreign matters into the product may also

result in malfunctions. If liquids or foreign matters enter the product, do not use the product, and

contact your nearest Nikon representative.

3. Cautions on moving the product

•When carrying the microscope, hold the microscope firmly by the bottom

front recess and the top rear.

•When moving the product, do not hold by the focusing knobs, eyepiece tube,

and stage, etc. The parts may become detached and cause the product to

fall, and may also result in malfunctions and loss of precision.

4. Cautions on assembling the product

•Take care to avoid pinching your fingers and hands.

•Scratches and dirt (i.e. fingerprints) on optical components such as lenses and filters will

degrade the microscope image. When assembling the product, be careful not to scratch or

directly touch the optical components.

5. Stage rack

The stage rack will protrude when the stage is operated. Be careful

not to hit the rack with your hand or other parts of your body when

handling the microscope as you may get hurt by an edge of the

rack.

6. Disposal of the microscope

To avoid biohazard risks, dispose of the microscope as contaminated equipment, according to

the standard procedures for your facility.

Rack

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