Nikon Eclipse Ti-E Bedienungsanleitung

Inverted Microscope

Instructions

M450 E 07.10.NF.1

Introduction

Thank you for purchasing a Nikon product.

This instruction manual is written for users of Nikon Inverted Microscope Eclipse Ti-E and Ti-E/B.

To ensure correct usage, read this manual carefully before operating the product.

•No part of this manual may be reproduced or transmitted in any form without prior written permission from

Nikon.

•The contents of this manual are subject to change without notice.

•Although every effort has been made to ensure the accuracy of this manual, errors or inconsistencies may

remain. If you note any points that are unclear or incorrect, please contact your nearest Nikon

representative.

•Some of the equipment described in this manual may not be included in the set you have purchased.

•If you intend to use any other equipment with this product, read the manual for that equipment too.

•When using TI-HUBC/A Hub Controller A with the microscope, also refer to the instructions manual

provided with the hub controller.

•If the equipment is used in a manner not specified by the manufacturer, the protection provided by the

equipment may be impaired.

Safety Precautions

To ensure correct and safe operation, read this manual before using the product.

Warning and Caution Symbols in this Manual

Although this product is designed to be completely safe during use, incorrect usage or failure to follow the

safety instructions provided may cause personal injury or property damage. To ensure correct usage, read

this manual carefully before using the product. Do not discard this manual and keep it handy for easy

reference.

Safety instructions in this manual are marked with the following symbols to highlight their importance. For

your safety, always follow the instructions marked with these symbols.

Symbol Description

Warning Disregarding instructions marked with this symbol may lead to serious injury or death.

Caution Disregarding instructions marked with this symbol may lead to injury or property

damage.

Symbols on the Product

The symbols on the product indicate the need for caution at all times during use. Before operating a part

labeled with the following symbols, refer to the instruction manual and read the relevant instructions.

Symbol Meaning

CAUTION: HOT

This symbol can be found on the top of the dia pillar illuminator and on the 12V 100W

lamphouse, and cautions the following:

•The lamp and the lamphouse will be extremely hot while and immediately after using

the lamp.

•To avoid the risk of burns, do not touch the lamp and the lamphouse while or

immediately after using the lamp.

•When replacing the lamp, wait for the lamp and the lamphouse to cool sufficiently.

Biohazard

This symbol can be found on the upper part of the microscope, and cautions the

following:

•The product may become biohazardous if a specimen is spilled onto the product.

•To avoid exposure to biohazard, do not touch contaminated parts with your bare

hands.

•Decontaminate the contaminated parts according to the standard procedures for your

facility.

General Caution

This symbol can be found on the top of the nosepiece protection plate on Ti-E and

Ti-E/B, and on top of the PFS6 nosepiece protection plate on the TI-ND6-PFS PFS

Motorized Nosepiece, and cautions the following:

•To avoid injury (i.e. by getting your fingers caught), be sure to attach the protection

plate correctly.

Safety Precautions

LED Safety

The PFS Motorized Nosepiece (TI-ND6-PFS Perfect Focus Unit) uses light in the near-infrared region (IR

wavelength) emitted from an infrared LED to control the focus. When using the PFS Motorized Nosepiece

with the product, the microscope system conforms to the EU standard EN60825-1: 2001 and the

international standard IEC60825-1: 2001. The product is classified as a Class 1 LED product.

Attempting to control or adjust the product in manners not described in this manual may result in exposure to

hazardous LED light.

Safety Labels on TI-ND6-PFS Perfect Focus Unit

•Safety labels are affixed to the bottom of the TI-ND6-PFS Perfect Focus Unit.

CLASS 1 LED PRODUCT

IEC/EN60825

1 :

2001

CAUTION

CLASS 1M INVISIBLE

LED RADIATIONWHEN OPEN

DO NOT VIEW DIRECTRLY WITH

OPTICAL INSTRUMENTS

593901

JAPAN

ND6

PFS

PFS Motorized Nosepiece (bottom view)

(1) Caution label

CAUTION

CLASS 1M INVISIBLE

LED RADIATION WHEN OPEN

DO NOT VIEW DIRECTRLY WITH

OPTICAL INSTRUMENTS

(2) Class 1 LED product label

CLASS 1 LED PRODUCT

IEC/EN60825

1 :

2001

(3) Safety standards label

593901

JAPAN

ND6

PFS

•Note that under normal use, infrared light is emitted from the position shown in the figure below.

OUT

DICHROIC MIRROR

Infrared li

ht emission

ort

(3) Safety standards label (2) Class1 LED

product label

(1) Caution label

Safety Precautions

Warning

1. Intended application of the product

The product is intended mainly for microscopic observations and micromanipulations of living

cells and tissues under diascopic or episcopic illumination. It is designed for the purposes of

experimentations and observations at hospitals or laboratories in the fields of genetics,

immunology, physiology, pharmacology, neurology, cellular biology, and molecular biology.

The product is classified as an in-vitro diagnostic medical device.

2. Do not disassemble.

Disassembling this product may result in electric shock or malfunction. Malfunctions and damage

due to such mishandlings will not be warranted. Do not disassemble any part that is not indicated

in this manual. If you experience problems with the product, contact Nikon.

3. Read the instructions thoroughly.

To ensure safety, thoroughly read this manual and the manuals for other equipment to be used

with this product. In particular, be sure to follow the warnings and cautions at the beginning of the

manuals.

4. Check the input voltage.

The microscope body uses an AC adapter as its power source. The dia illumination lamp uses

one of two types of power supply devices.

• AC adapter for the microscope body:

The AC adapter for the microscope can be used with 100 to 240 VAC at 50-60 Hz, and can be

used with most wall outlets in the world. Under normal use, you will not need to pay particular

attention to the supplied power voltage.

• TI-PS100W Power Supply:

TI-PS100W Power Supply can be used with 100 to 240 VAC at 50/60 Hz, and can be used

with most wall outlets in the world. Under normal use, you will not need to pay particular

attention to the supplied power voltage.

• TE-PS30W Power Supply A or TE-PSE30 Power Supply A:

The input voltage ratings are indicated on the rear panel of the power supply device. Before

connecting the power cord, check that the indicated input voltage matches the voltage of the

wall outlet. If the indicated input voltage does not match your regional voltage supply, do not

use the power supply device, and contact Nikon. Use of a power supply device with the

inappropriate voltage rating may result in overheating or fire due to overcurrent, and may also

cause damage the power supply device and connected devices.

5. Use the specified AC adapter only.

The product is powered by an AC adapter. Be sure to use the specified AC adapter with the

product. Use of other AC adapters may result in malfunction, overheat, or fire.

•See Chapter 7, “Specifications” for the specified AC adapter.

•Place the AC adapter in a well-ventilated area to prevent malfunction and fire. Obstacle

covering or placed on the AC adapter may hinder the dissipation of heat and cause the AC

adapter to become abnormally hot.

•To prevent malfunctions and errors, turn off the POWER switch on the Ti-E or Ti-E/B

microscope body (set the POWER switch to the “OFF” side) before connecting the AC

adapter.

Safety Precautions

Warning

6. Cautions on the power cord

Be sure to use the specific power cords for the AC adapter and the power supply device. Use of

other power cords may result in malfunction, overheat, or fire.

•See Chapter 7, “Specifications” for the specified power cord.

•To prevent electric shock, turn off the power switches on the Ti-E or Ti-E/B microscope body

and on the power supply device before connecting or disconnecting the power cord. On Ti-E

and Ti-E/B, set the POWER switch to the “OFF” side. On the power supply, set the POWER

switch to the “O” side.

•Note that the power supply device is classified as Class I for electric shock protection. Be sure

to connect it to a protective ground terminal.

7. Check the combination of dia pillar illuminator, lamp, and power supply device.

The combination of the dia pillar illuminator and the power supply device is specified based on

the lamp ratings (12V/100W or 6V/30W) and the power voltage. Use them in the correct

combination according to the instructions on page 55. Use of the devices in a wrong combination

may result in malfunction, overheat, or fire.

8. Cautions on heat from the light source

The lamp and the lamphouse become extremely hot when the lamp is turned on. Follow the

cautions below to prevent burns and fire.

•To avoid burns, do not touch the lamp and the lamphouse while the lamp is on or for

approximately thirty minutes after it has been turned off.

•To avoid the risk of fire, do not place fabric, paper, or highly flammable volatile materials (i.e.

gasoline, petroleum benzine, paint thinner, and alcohol) near the lamphouse while the lamp is

on or for about thirty minutes after it has been turned off.

•Do not block the air vents on the lamphouse. If any obstacle covers the lamphouse or

something is placed on the lamphouse, the heat dissipation is inhibited and the lamphouse

becomes abnormally hot.

•The bottom of the power supply device becomes hot during use. Do not cover the air vents on

the side of the power supply device.

9. Cautions on lamp replacement

•When replacing the lamp, wait approximately thirty minutes after turning off the lamp, and

make sure that the lamp and the lamphouse have cooled sufficiently.

•To prevent electric shock and product damage, turn off the power switch on the power supply

(set the POWER switch to the “O” side) and unplug the power cord from the wall outlet before

replacing the lamp.

•After replacing the lamp, close and secure the lamphouse cover. Never use the product with

the lamphouse cover left open.

•Do not break the used lamp. It should be disposed of as an industrial waste, according to the

local regulations and rules.

Safety Precautions

Warning

10. Cautions on motorized devices

The product can rotate and focus (raise and lower) the PFS Motorized Nosepiece with motors.

The product can also have various motorized devices attached. When TI-HUBC/A Hub Controller

A is used with the product, the system can be controlled with a TI series controller or a PC.

To prevent injuries, pay attention to the following when operating motorized devices:

•Before operating the product, check the whole microscope system, and make sure that all

moving parts can be operated safely.

•Your hands and fingers may be caught and injured by the nosepiece, objectives, stage, parts

arranged on the stage, or specimen containers. Keep your hands away from these devices

during operation.

•Check the optical path of the whole microscope system before operating the shutter on the

illuminator or adjusting the brightness. The light source emits a very strong light. If the optical

path is not set up correctly, the illumination light may leak out or enter the objective, and

cause an eye injury.

11. Notes on handling hazardous specimens

The product is intended mainly for microscopic observations and micromanipulations of

specimens such as living cells and tissues in a Petri dish. Note the following when handling the

specimens:

•Before handling a specimen, check whether it is hazardous. Wear rubber gloves when

handling hazardous (i.e. potentially infectious) specimens.

•Be careful not to spill the specimen. If a specimen is spilt onto the microscope, decontaminate

according to the standard procedures for your facility.

12. Notes on handling flammable solvents

The following flammable solvents are used with the product:

•Immersion oil (Nikon Immersion Oil for oil immersion objectives)

•Absolute alcohol (ethyl alcohol or methyl alcohol for cleaning optical parts)

•Petroleum benzine (for wiping off the immersion oil)

•Medical alcohol (for disinfecting the microscope)

Never hold a flame near these solvents. When using a solvent, thoroughly read the instructions

provided by the manufacturer, and handle correctly and safely. Note the following precautions

when using solvents with the product.

•Keep solvents away from the lamp, the lamphouse, the power supply device, and any other

parts that may become hot.

•Keep solvents away from the product and its surroundings when turning on/off the power

switch or plugging/unplugging the power cord.

•Be careful not to spill the solvents.

Safety Precautions

Caution

1. Turn off the power during installation, assembly, connection/disconnection of cables,

and maintenance.

To prevent electric shock and fire, be sure to turn off the power switch on the microscope body

and the power supply device (set the POWER switches to the “OFF” side or the “O” side) and

unplug the power cords before installing or assembling the product, connecting or disconnecting

cables, replacing the lamp, or performing maintenance tasks such as cleaning of the lenses.

2. Do not wet the product or allow intrusion of foreign matters.

Do not wet or spill liquids onto the product, as it may result in malfunction, overheat, or electric

shock. If water or other liquids are accidentally spilled onto the product, immediately turn off the

power switch (set the POWER switch to the “OFF” side or the “O” side) and unplug the power

cord from the wall outlet. Then, wipe off the liquid with a piece of dry cloth. Intrusion of foreign

matters into the product may also result in malfunctions. If liquids or foreign matters enter the

product, do not use the product, and contact Nikon.

3. Weak electromagnetic waves

The product emits weak electromagnetic waves. Do not install the product near precision

electronic devices to avoid affecting their performance. If signal reception by a TV or radio is

affected, move the TV or radio set away from the product.

4. Cautions on moving the product

•When carrying the product, hold the product firmly by the bottom front recess and the bottom

rear.

•When moving the product, do not hold by the focusing knobs, eyepiece tube, stage, or dia

pillar illuminator. The parts may become detached and cause the product to fall, and may also

result in malfunctions and loss of precision.

5. Cautions on assembling the product

•Take care to avoid pinching your fingers and hands.

•Scratches and dirt (i.e. fingerprints) on optical components such as lenses and filters will

degrade the microscope image. When assembling the product, be careful not to scratch or

directly touch the optical components.

6. Cautions on the protruding rack of the Rectangular Mechanical Stage

The stage rack of TI-SR Rectangular Mechanical Stage will protrude when the stage is operated.

When operating the focus knobs or the nosepiece, be careful not to strike your hands against the

rack. Contact with the edges of the rack may result in injury.

7. Disposal

To avoid biohazard risks, dispose of the product as contaminated equipment, according to the

standard procedures for your facility.

Safety Precautions

Notes on Handling the Product

1. Handle with care.

The product is a precision optical instrument. Handle the product with care and avoid physical shocks

and vibrations.

In particular, the precision of objectives may be lost by even weak physical shocks.

2. Installation location and storage location

The product is a precision optical instrument. Use or storage of the product under inappropriate

conditions may result in malfunctions or loss of precision. The following conditions must be considered

for the installation location and the storage location.

•Install the product in a location with a temperature of 0 to 40°C and a relative humidity of 85% or

less (no condensation).

Store the product in a location with a temperature of -20 to 60°C and a relative humidity of 90% or

less (no condensation).

Use or storage of the product in a hot or humid location may result in molding of or condensation on

the lenses, loss of precision, and malfunctions.

•Install the product in a place with little dust and dirt.

When storing the product, place a cover over the product to protect it from dust.

•Install the product in a place with little vibration.

•Install and store the product on a level and sturdy table or stage that can bear the weight of the

product.

Install the product in a location with minimal exposure to hazards in the event of earthquakes and

other potential disasters. If necessary, secure the product to the working desk or other heavy and

stable items with a strong rope or other means, so as to prevent it from falling.

•Avoid placing the product in direct sunlight or immediately under the room lights.

The image quality is degraded in a bright environment due to the extraneous light entering the

objective. A room light immediately above the microscope may also enter the objective as

extraneous light, especially when using a condenser lens with a longer working distance (i.e. ELWD

and LWD). In this case, it is recommended that you turn off the room light above the microscope.

•Install the product at least 10 cm away from the surrounding walls.

When using TI-DH Dia Pillar Illuminator 100W, install the product at least 15 cm away from the wall,

so that the caution labels on the dia pillar illuminator and the lamphouse are visible. Note that TI-DH

Dia Pillar Illuminator 100W can be inclined backward to secure working space. To utilize this function,

install the product so that there is enough space between the product and the wall for the dia pillar

illuminator to be inclined.

•Do not install the product in a closed space such as a locker or a cabinet.

•Do not place items on the product.

•Install the product so that the power cords to the AC adapter and the power supply can be

unplugged immediately in case of an emergency.

3. Notes on handling optical parts

Scratches and dirt (i.e. fingerprints) on optical components such as lenses and filters will degrade the

microscope image.

Handle the optical components with care, so as not to damage them. If they require cleaning, see

Chapter 6, “Daily Maintenance.”

4. Notes on handling the lamp

Do not touch the lamp glass with your bare hands. Fingerprints and other dirt on the lamp may result in

uneven illumination and reduce the service life of the lamp. Wear gloves or use other pieces of clothes

when handling the lamp.

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