Olympus IX81 Bedienungsanleitung

INSTRUCTIONS

IX81

MOTORIZED INVERTED

RESEARCH MICROSCOPE

A X 7 3 2 0

This instruction manual is for the Olympus Motorized Inverted Research Microscope Model IX81.

To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of

this microscope, we recommend that you study this manual thoroughly before operating the

microscope. Retain this instruction manual in an easily accessible place near the work desk for

future reference.

IX81

CONTENTS

7-10

11-12

13-27

IMPORTANT — Be sure to read this section for safe use of the equipment. —

1 MODULE NOMENCLATURE

2 CONTROLS

4 USING THE CONTROLS

TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION PROCEDURE

4-1 Microscope Frame .................................................................................................................................................................. 13-14

4-2 Focusing Block ...................................................................................................................................................................................... 15

4-3 Stage............................................................................................................................................................................................................ 16-17

4-4 Observation Tube ..................................................................................................................................................................... 18-20

1-5

Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to

assemble the microscope yourself, please read Chapter 9, “ASSEMBLY” (pages 49 to 58) carefully. For the modules

provided with instruction manuals, also read the assembly procedures in their instruction manuals.

When the IX81 motorized revolving nosepiece is rotated in stand-alone mode (without a PC), automatic es-

cape movement of an objective (prevention of interference between the objective and stage) is not performed.

Therefore, when using an objective with a short working distance, let the motorized revolving nosepiece

escape once before pressing the objective switching button.

1Voltage Indication 2Light Path Selection

3Magnification Change 4Motorized Revolving Nosepiece

5Frame Clamping Plate

1Adjusting the Interpupillary Distance 2Adjusting the Diopter

3Using the Eye Shades 4Using Eyepiece Micrometer Disks

5Selecting the Light Path of Observation Tube (U-TR30H Only)

6Using the CT Turret (U-BI90CT Only) 7Adjusting the Tilt (U-TBI90 Only)

1Placing the Specimen 2Moving the Specimen

3Connecting the Grounding Wire 4

Adjusting the X-Axis/Y-Axis Knob Rotation Tension

Rotation Direction of the Focusing Knob

2Coarse/Fine Adjustment Switching Button

3Focusing (UP/DOWN) buttons 4

Objective Escape/Return (ESC) Button

Note on operation in stand-alone mode

4-5 Illumination Column (IX2-ILL100) ...................................................................................................................... 20-22

1Tilting the Illumination Column 2Mounting Filters

3Using the Field Iris Diaphragm

4 Adjusting the Condenser Height Adjustment Knob Tension

5 Caution on Attachment of the Manipulator

IX81

5 OTHER OBSERVATION METHODS

5-1 Phase Contrast Observation .................................................................................................................................. 28-31

5-2 Differential Interference Contrast Observation............................................................................. 32-36

5-3 Simplified Polarized Light Observation ............................................................................................................. 37

5-4 Reflected Light Fluorescence Observation (Separate Manual).................................... 37

6 PHOTOMICROGRAPHY AND TV OBSERVATION 38-42

ASSEMBLY

—

See this section for the replacement of the light bulb. —

PROPER SELECTION OF THE POWER SUPPLY CORD ...................................................................... 59-60

28-37

6-1 Photomicrography.................................................................................................................................................................. 38-40

6-2 TV Observation............................................................................................................................................................................. 41-42

7 TROUBLESHOOTING GUIDE

8 SPECIFICATIONS

43-45

46-48

49-58

4-6 Condenser......................................................................................................................................................................................... 23-25

4-7 Objectives........................................................................................................................................................................................... 26-27

1Centering the Condenser 2Using the Aperture Iris Diaphragm

3Flipping Up the Condenser Holder

1Oil-Proof Cap (UIS Series only) 2Adjusting the Correction Collar

3Using Immersion Objectives

NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device,

pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection

against harmful interference when the equipment is operated in a commercial environment. This

equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in

accordance with the instruction manual, may cause harmful interference to radio communications.

Operation of this equipment in a residential area is likely to cause harmful interference in which case

the user will be required to correct the interference at his own expense.

FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance

could void the user’s authority to operate the equipment.

This device complies with the requirements of directive 98/79/EC concerning in vitro diagnostic

medical devices. CE marking means the conformity to the directive.

10 LAMP HOUSING INSPECTION SHEET 61

IMPORTANT

The IX81 microscope can be used in stand-alone mode (operated from the U-HSTR2 hand switch) or can operated from a

PC when the IX2-BSW software (Ver. 01.03, compatible with Windows 2000/Me) is installed in the PC.

Be sure to prepare the U-ZPCB (T2) Z-board and attach it to the IX81, and set the DIP switch (see page

50) before connecting the IX2-UCB control board to the IX81.

This microscope employs a UIS2/UIS (Universal Infinity System) optical design, and should be used only

with modules designed for the IX2 or BX2 series (which belong to the Olympus IX or BX series).

For the applicable modules, please consult Olympus or the latest catalogues. Less than optimum per-

formance may result if inappropriate module combinations are used.

Configuration of Instruction Manuals

Since this microscope is expandable to a variety of systems, separate instruction manuals are prepared so

that the user has to read only the manuals according to the user’s own system.

Manual Name Main Contents

IX81 Observation procedures including transmitted light brightfield,

phase contrast and DIC observations

IX2-UCB/U-HSTR2 Functions of the Control Box (incorporating the power supply)

and Hand Switch

IX2 Software for PC (CD-ROM)

*Model name IX2-BSW (Ver. 01.03 or later)

Methods of PC control of functions

Fluorescence system for IX2 Reflected light fluorescence observation

U-FWT/FWR/FWO

Motorized filter wheels (The U-FWT cannot be used with this microscope.)

Fig. 1

Precautions When Unpacking the Microscope

Releasing the Transport Lock of the Motorized Revolving Nosepiece

#Never attempt to turn the system on without removing the clamping

rod. Otherwise, the focusing mechanism may be damaged.

1. Loosen the screw @of the clamping rod using the Allen screwdriver

provided with the microscope frame.

2. Turn the nut ² of the clamping rod in the direction of the arrow until the

nut reaches the lowest height.

3. Likewise, turn the clamping rod ³ in the direction of the arrow until the

rod reaches the lowest height.

4. Insert the Allen screwdriver into the hole on the clamping rod mount 4

and turn the screwdriver in the opposite direction to the dial to remove

the clamping rod.

5. Attach the provided seal (10 mm dia., white) on the hole made after

removing the clamping rod to prevent penetration of dust through the

hole.

}Retain the clamping rod carefully because it will be used again the next

time the microscope is transported.

IX2-MLWCD

IX2-DICD DIC condenser

Mid long working distance condenser

CAUTION

IX81

1. After the equipment has been used in an observation of a specimen that

is accompanied with a potential of infection, clean the parts coming in

contact with the specimen to prevent infection.

· Moving this product is accompanied with the risk of dropping the

specimen. Be sure to remove the specimen before moving this

product.

· In case the specimen is damaged by erroneous operation, promptly

take the infection prevention measures.

2. If a foreign object is caught during motorized focusing operation, there

will be an error in the focusing block and the motorized focusing opera-

tion will be suspended.

Recovery procedure

· If there is no error in motorized operation, the caught object can be

removed by turning the focusing knob or focusing button.

· If there is an error in motorized operation, the focusing knob

or focusing button becomes inoperable. Disassemble the

relevant modules to remove the caught object. Replace the

relevant modules afterward.

· Turn off the power and then on again. The system will restart unless

there is a malfunction in the motor.

3. To activate temporary stop during focusing operation, turn the focusing

knob (or dial) on the microscope frame (in either direction) or press any of

the FOCUS control buttons ( , , F/C and ESC) (except during PC

communications).

When the main switch of the IX2-UCB control box is set to “ I" (ON),

the focusing operation starts automatically as part of initialization

(this operation consists of temporary raising and then returning to

the original position of the objective).

If any of the operations mentioned above is performed, an emergency

stop will also occur. In this case, set the main switch to “ " (OFF) and

then “ I" (ON) again.

4. When lowering the objective by pressing the focus button, be careful

not to have your hand caught between the bottom of the revolving

nosepiece and the microscope frame.

SAFETY PRECAUTIONS

Releasing the Transport Lock of the Light Path Selector

#Never attempt to operate the light path selector without removing the

transport lock knob 5. Otherwise, the light path selector mechanism

may be damaged.

· Rotate the knob counterclockwise to remove it.

· To prevent penetration of dust through the hole made after removing the

transport lock knob, stop the hole by attaching the provided seal (10 mm

dia., black).

}Retain the knob carefully because it will be used again the next time the

microscope is transported.

Stage IX2-SFR/IX-MVR

· Before transporting the stage, fix the flexible handle with pieces of adhesive

tape so that it will not move.

Fig. 2

5. The microscope is provided with a simplified waterproof mechanism.

Therefore, if culture liquid or water is spilt on the stage, revolving nosepiece

or microscope frame, damage to the equipment or an electrical shock

may result. Immediately wipe the liquid or water off if it is spilt on them.

6. When moving the microscope, remove the modules that may drop

including the specimen from the microscope in advance, then care-

fully carry the microscope frame by the base (front edge) 1and the

grasping part on the illumination column 2as shown in Fig. 2 (Weight:

approx. 25 kg).

When moving the microscope for a long distance, it is also recommended

to disconnect all cables from the equipment.

When transporting it, also engage the transport lock mechanisms and

package it sufficiently.

Also be careful against slipping of hands during carrying.

#Damage to the microscope will occur if you grasp it by other parts

including the stage, focusing knob, etc.

7. The microscope is not covered by warranty in terms of laser safety. The

user should assume liabilities for any consequence of user modification

including introduction of the use of laser beam.

8. The surfaces of the lamp housing will become extremely hot during

operation. When installing the microscope, make sure to allow ample free

space (10 cm or more) around and in particular above the lamp housing.

9. When installing the microscope, route the power cord away from the

lamp housing. Should the power cord come in contact with the hot lamp

housing, the power cord could melt and cause electric shock.

Fig. 3

Designated Bulb 12V100WHAL-L (PHILIPS 7724)

11. Always use the power cord provided by Olympus. If no power cord is

provided, please select the proper power cord by referring to the section

“PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of

this instruction manual. If the proper power cord is not used, product

safety performance cannot be warranted.

12. Always ensure that the grounding terminal of the microscope and that

of the wall outlet are properly connected. If the equipment is not grounded,

Olympus can no longer warrant the electrical safety performance of the

equipment.

13. Never insert metallic objects into the air vents of the microscope frame

as this could result in electrical shock, personal injury and equipment

damage.

14. The standard service life of the lamp housing is 8 (eight) years of use or

20,000 hours of total power ON period, whichever is the shorter period.

For details, see Inspection Sheet on page 61.

10.To avoid potential shock hazards and burns when replacing the light

bulb, set the main switch to “ ” (OFF) then disconnect the power cord

from the wall outlet in advance. Whenever you replace the bulb during

use or right after use, allow the lamp housing 3and bulb to cool before

touching. (Fig. 3)

IX81

Safety Symbols

Symbol Explanation

Indicates that the surface becomes hot, and should not be touched with bare hands.

Indicates that the main switch is ON.

Indicates that the main switch is OFF.

Warnings

The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment

in the safest possible manner.

When raising or lowering the motorized revolving nosepiece, be careful not to have your finger

or hand caught by the mechanism.

Before use, carefully read the instruction manual. Improper use could result in personal injury to

the user and/or damage to the equipment.

Warning engravings are placed at parts where special precaution is required when handling and using the microscope.

Always heed the warnings.

Warning engraving

position

Lamp housing

(U-LH100L-3, U-LH100-3, IX-HLSH100)

(High Temperature

warning)

Right side panel of microscope (Warning against finger or hand

catching by mechanism)

1Getting Ready

1. A microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact.

2. Do not use the microscope where it is subjected to direct sunlight, high temperature and humidity, dust or vibrations. (For

operating conditions, see Chapter 8, “SPECIFICATIONS” on page 48).

3. An intermediate attachment with a thickness of up to 60 mm can be mounted between the microscope frame and

binocular observation tube (U-BI90CT, U-BI90).

· If the U-BI90CT is used together with an intermediate attachment, the image may be cut off or obscured.

4. The oil-proof cap can only be mounted on a UIS Series 3 objective. Note that this does not change optical performance.

(For applicable objectives, see page 26.)

5. Restrictions in brightfield, phase contrast and DIC observations

· Deterioration in the optical performance will occur when the optional 2X magnification changer is installed in the

U-BI90CT.

(The deterioration can be improved by adding the U-EPA2 eye-point adjuster.)

· With the combination of U-TR30H + U-FWO, the full optical performance may not be able to be manifested with the

objectives listed in 1below and it is not possible to use the objectives listed in 2below. (This applies to objectives

other than Series 3.)

UIS2 Series

1UPlanSApo4X, 10X2, UPlanFLN4X, 10X2, 20X, CPlanFLN10X, LUCPlanFLN20X, UPlanSApo100XO

2PlanN40X, PlanApoN60XO

UIS Series

1UPlanApo4X, 10X, UPlanFl4X, 10X, 20X, CPlanFl10X, LCPlanFl20X, UPlanApo100XOI3

2Plan40X, UPlanApo40XOI3/340, PlanApo60XO3

6. Restrictions in TV observation

@The following combinations are not permitted in consideration of the optical performance.

· IX2-SPT + PE4X + U-PMTVC on the side port

· IX2-SPT + PE5X + 1X magnification changer + U-PMTV1X on the side port

· U-TV0.35XC + 2X magnification changer (optional)

· U-TV0.35XC + DP50 (optional 2X magnification changer on the side port)

²The following combination may deteriorate the optical performance a little.

· U-TV0.35XC + DP50 (UPlanSApo/UPlanApo4X/10X + 1X magnification changer on the side port)

7. Restrictions in fluorescence observation

· With combination of IX2-SHA + U-FWR (x 2) + U-LH100HGAPO, objectives UPlanSApo40X2, UPlanApo40X, UPlanFLN/

UPlanFl20X, UPlanFLN100XO2/100XOI2, UPlanFl100XO3, PlanApoN60XO and PlanApo60XO3 cannot be used due to

a problem in the optical performance. (This applies to objectives other than Series 3.)

8. Other

· The U-TRU or U-TVCAC cannot be mounted on the side port.

· When a large module is attached to the U-TR30H straight photo tube, it will be difficult to confirm the specimen.

· Only either the lower back port or left side port can be used.

2Maintenance and Storage

1. To clean the lenses and other glass components, simply blow dirty away using a commercially available blower and

wipe gently using a piece of cleaning paper (or clean gauze).

If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute

alcohol.

Since the absolute alcohol is highly flammable, it must be handled carefully.

Be sure to keep it away from open flames or potential sources of electrical sparks –– for example, electrical

equipment that is being switched on or off.

Also remember to always use it only in a well-ventilated room.

2. Do not attempt to use organic solvents to clean the non-optical components of microscope. To clean them, use a lint-free,

soft cloth slightly moistened with a diluted neutral detergent.

3. Never attempt to disassemble any part of the microscope.

4. When not using the microscope, make sure to set the main switch to “ ” (OFF), confirm that the lamp housing is cool

enough and cover the microscope with the provided dust cover.

If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition,

the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.

3Caution

The following symbols are used to set off text in this instruction manual.

: Indicates that failure to follow the instructions in the warning could result in bodily harm to the

user and/or damage to equipment (including objects in the vicinity of the equipment).

# : Indicates that failure to follow the instructions could result in damage to equipment.

} : Indicates commentary (for ease of operation and maintenance).

4Intended use

This instrument has been designed to be used to observe magnified images of specimens in routine and research

applications.

Do not use this instrument for any purpose other than its intended use.

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