Optika B-500 Bedienungsanleitung

microscopes

i T A L Y

OPTIKA MICROSCOPES - ITALY

www.optikamicroscopes.com - [email protected]

Ver. 4.1.0

B-500

OPERATION MANUAL

GUIDA UTENTE

MANUAL DE INSTRUCCIONES

MANUEL D’INSTRUCTIONS

BEDIENUNGSANLEITUNG

Page 2

INDEX

1.0 DESCRIPTION page 4

2.0 INTRODUCTION page 7

3.0 UNPACKING AND ASSEMBLY page 7

4.0 USING THE MICROSCOPE page 8

5.0 MAINTENANCE page 10

6.0 ELECTRICS page 11

7.0 RECOVERY AND RECYCLING page 11

Page 3

This microscope is a scientific precision instrument designed to last for many years with a minimum of main-

tenance. It is built to high optical and mechanical standards and to withstand daily use.

Optika reminds you that this manual contains important information on safety and maintenance, and that it

must therefore be made accessible to the instrument users.

Optika declines any responsibility deriving from instrument uses that do not comply with this ma-nual.

Safety guidelines

This manual contains important information and warnings regarding safety about installation, use and

maintenance of the microscope. Please read this manual carefully before using the equipment. To

ensure safe use, the user must read and follow all instructions in this manual. OPTIKA products are

designed for safe use in normal operating conditions. The equipment and accessories described in

the manual are manufactured and tested according to industry standards for safety instrumentation

laboratory. Misuse can cause personal injury or damage to the instrument. Keep this manual at hand

close to the instrument, for an easy consultation.

Electrical safety

Before connecting the power cord to wall outlet, ensure that your mains voltage for your region corre-

sponds to the voltage supply of the instrument, and that the illuminator’s switch is in position OFF. The

user must observe the safety regulations in force in his region. The instrument is equipped with CE sa-

fety marking, in any case the user has full responsibility concerning the safe use of that instrument.

Warning/Caution symbols used in this manual

The user should be aware of safety aspects when using the instrument. Warning or hazard symbols

are shown below. These symbols are used in this manual.

The instructions on this symbol to avoid possible severe personal injuries.

Warning of use; the incorrect operation on the instrument can cause damages

to the person or instrument.

Possibility of electric shock.

Attention: high temperature surfaces. Avoid direct contact.

Technical notes or usage tips.

SAFETY GUIDELINES

Page 4

1.0 DESCRIPTION

Photo port

(Only on trinocular

microscopes)

Observation head

Main body

Objective

Slide clamp

Coarse focusing

knob

Fine focusing knob

Focus-stop knob

Brightness

adjustment knob

Field diaphragm

Iris diaphragm

Stage

Nosepiece

Eyepieces

Page 5

1.0 DESCRIPTION

Light path selector

lever

(Only on trinocular

microscopes)

Analyser holder

Condenser

Condenser-centring

screws

Translation knobs

Condenser

adjustment

knob

Tension

adjustment

knob

Dioptric adjustement ring

Page 6

1.0 DESCRIPTION

Ergonomical binocular

head

B-500 ERGO MODEL

White LED

illuminator

Page 7

This microscope is a scientific precision instrument designed to last for many years with a minimum

of maintenance. It is built to high optical and mechanical standards and to withstand daily classroom

and laboratory use.

Optika reminds you that this manual contains important information on safety and maintenance, and

that it must therefore be made accessible to the instrument users.

Optika declines any responsibility deriving from instrument uses that do not comply with this ma-

nual.

3.0 UNPACKING AND ASSEMBLY

2.0 INTRODUCION

3.1 The microscope is housed in a moulded Styrofoam container. Remove the tape from the

edge of the container and lift the top half of the container. Take some care to avoid that the

optical items (objectives and eyepieces) fall out and get damaged. Using both hands (one

around the arm and one around the base), lift the microscope from the container and put it on

a stable desk.

3.2 The objectives are located in individual protective vials. Remove the objectives from the vials

and insert them into the microscope nosepiece in the order from the lowest magnification to

the highest, in a clockwise direction from the rear.

3.3 Insert the eyepieces into the eye tubes. Place the observation head onto the top of the arm

and tighten the lock-screw. If you have a trinocular microscope and want to use the photo

port, insert your adaptor in the hole on the top of the observation head and fixate it with the

screw on the side.

Connect the mains plug into the socket at the base

Make sure, before you turn the illumination on, that the voltage selector is set to the mains voltage for

your region.

The power cord should be used only on network sockets equipped with adequate grounding.

Contact a technician to check the state of your electrical system. If there is no need to install additional

accessories, the instrument is now ready for use. Once positioned and installed with the necessary

components, the microscope is ready to be used. Your microscope is a laboratory instrument desi-

gned to last. Handle it always carefully and avoid abrupt vibrations or shocks. Always disconnect the

power cable from the microscope when not in use for long time, while you clean it or when you perform

any maintenance.

AVOID DISASSEMBLING THE INSTRUMENT

Do not disassemble the instrument. This entails the cancellation of the warranty and may cause

malfunction.

Page 8

4.1 Adjust the observation head

Loosen the lock-screw, turn the observation head to a comfortable position for observation,

and then lock the lock-screw.

4.2 Place the specimen on the stage

Lock the specimen slide on the mechanical stage using the slide clamp. Ensure that the spe-

cimen is centred over the stage opening by adjusting the coaxial knobs of the stage.

4.3 Lamp settings

The microscope is fitted with a 20w illuminator (3w high-efficiency white led source for b-500

ergo model) . Insert the plug of the cable into the power socket and turn on the switch on

the back of the main body. Turn the brightness adjustment knob to a brightness suitable for

observation.

4.4 Adjust interpupillary distance

Hold the right and left parts of the observation head by both hands and adjust the interpu-

pillary distance by turning the two parts until one circle of light can be seen. After finishing

the interpupillary adjustment, turn the dioptric adjustment ring on the left eye tube to the “0”

position at the graduation line on the eyetube.

4.5 Focus and diopter adjustment

Loosen the focus-stop knob, rotate the coarse focusing knob to bring the slide into focus

with an objective with a low magnification, and then lock the focus-stop knob. Adjust the fine

focusing knob to get the image sharp and clear while observing with your right eye, then turn

the left dioptric adjustment ring to a sharp and clear image also with the other eye. Turn the

tension-adjust-knob to get a suitable tension for the focus system. When the image is in fo-

cus, turn the nosepiece to choose the objective you need.

4.6 Condenser adjustment

Turn the condenser adjustment knob to move the condenser up or down for a clear and

uniform sample illumination. The two condenser centring screws can be used to centre the

condenser.

4.7 Set the numerical aperture

Adjust the aperture of the iris diaphragm to set the numerical aperture of the illuminator, thus

controlling image contrast and resolution.

4.8 Field diaphragm

Adjust the field diaphragm to align the system and to control stray light.

4.9 Additional filters

The chromatic correction filter (blue) and the monochromator filter (green) can be inserted for

definition of specimen parts. The use of the green filter is recommended for phase contrast

observations.

4.10 Using the polarizer (optional)

The polarizing set consists of two filters; the analyser and the polarizer. The analyser is pla-

ced in the analyser holder and the polarizer on top of the illuminator.

4.0 USING THE MICROSCOPE

Page 9

4.0 USING THE MICROSCOPE

4.11 Video capturing (optional)

Two kinds of observation heads, binocular (standard or ergonomic) or trinocular, are availa-

ble for the b-500 series. Trinocular version has a dedicated photo-port, while binocular ver-

sion can receive a camera in either eyepiece tube, via a photo/video adaptor. Before taking

a picture or filming video, if necessary, obscure with a dark cloth both the camera viewfinder

and the eyepieces and pull out the light path selector lever. Please refer to the adaptor and

camera manuals for further details.

4.12 Using the phase contrast set (optional)

The phase contrast set has 4 objectives for phase contrast and one condenser, with 4 rings

of phase, and one position for bright field. To use the phase contrast, remove the abbe con-

denser of the microscope and replace it with the phase contrast condenser.

If, during use, the phase contrast diaphragm rings (anuli) are dislod-

ged, they can be realigned rotating the alignment screws placed on

two sides of each ring, using the enclosed tools. In particular:

Select an objective corresponding to the ring to be realigned, and

focus on a slide under bright field illumination.

The selector of the condenser has 5 selectable positions:

0 →

To use when it is necessary to be used the instrument in bright field

10 → To use with 10x phase contrast objective

20 → To use with 20x phase contrast objective

40 → To use with 40x phase contrast objective

100 → To use with 100x phase contrast objective

Insert the phase ring to be realigned.

Remove an eyepiece, and replace it with the centring telescope.

Focus the centring telescope, without touching the microscope

focus, in order to focus the phase ring and the phase plate simulta-

neously. In particular, the phase plate is visible as a dark ring, and

the phase ring as a bright ring divided into sectors.

Turning the two phase ring centring screws, position the phase ring

(bright ring) so that it is completely contained in the phase plate

profile (dark ring). (See figure.)

Remove the telescope, and replace the eyepiece.

4.13 Dark field condenser (optional)

For dark field observation, the Abbe condenser in standard equipment must be replaced

with a special condenser for dark field, showed in the picture.

Page 10

5.1 Always think about

- The following environment is required: Indoor temperature: 0-40°C, Maximum relative humi

dity: 85 % (non condensing).

- Keep the microscope away from dust and shocks while in use.

- Turn off the light immediately after use.

- Use a soft lens tissue to clean the optics after use.

- Only if needed, use a cloth moistened with water and a mild detergent, rinsing with water and

drying immediately with a lint-free cloth.

- After use, cover the microscope with the included dust-cover, and keep it in a dry and clean

place.

5.2 Do not!

- Wipe the surface of any optical items with your hands. Fingerprints can damage the optics.

- Use solvents, neither on the microscope, nor on the optics.

- Disassemble objective or eyepieces to attempt to clean them.

- Mishandle or impose unnecessary force on the microscope.

- Clean the unit with volatile solvents or abrasive cleaners.

- Attempt to service the microscope yourself.

5.3 How to change the lamp

- Before changing the lamp, you must pull the plug out of the electrical socket. To avoid being

burnt, wait until the lamp has cooled down.

- Hold the main body and tilt the microscope until the bottom base can be seen. Loosen the

door-lock screw of the small door on the bottom base to open the lamp housing. The lamp

and lamp holder come out with the door.

- Pull out the old lamp. Hold the new lamp without touching it with your bare hands (e.g. using

its packaging, or a cloth) and insert it into the holder. Replace/close the lamp holder.

B-500 ERGO: the high-efficiency white LED source in B-500 ERGO is designed to last many

years without any performance decrease. Never try to replace it with another kind of LED

source. In case of malfunctioning contact OPTIKA service.

5.4 If you need to send the microscope to Optika for maintenance, please use the original

packaging.

5.0 MAINTENANCE

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