Agilent Technologies 1100 Series Benutzerhandbuch

Agilent 1100 Series

Capillary LC System

System Manual

Notices

No part of this manual may be reproduced

in any form or by any means (including

electronic storage and retrieval or

translation into a foreign language)

without prior agreement and written

consent from Agilent Technologies, Inc. as

governed by United States and

international copyright laws.

Manual Part Number

G1388-90001

Edition

08/2002

Printed in Germany

Agilent Technologies Deutschland GmbH

Hewlett-Packard-Strasse 8

76337 Waldbronn, Germany

formance of this document or of any

information contained herein. Should

Agilent and the user have a separate

written agreement with warranty

terms covering the material in this

document that conflict with these

terms, the warranty terms in the

separate agreement shall control.

Technology Licenses

The hardware and/or software described

in this document are furnished under a

license and may be used or copied only in

accordance with the terms of such license.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly

performed or adhered to, could

result in personal injury or death.

Do not proceed beyond a

WARNING notice until the

indicated conditions are fully

understood and met.

Warning Symbols Used on the

Instrument

The apparatus is marked with this

symbol when the user should refer

to the instruction manual in order

to prevent risk of harm to the

Warranty

The material contained in this

document is provided “as is,” and is

subject to being changed, without

notice, in future editions. Further, to

the maximum extent permitted by

applicable law, Agilent disclaims all

warranties, either express or implied,

with regard to this manual and any

information contained herein,

including but not limited to the

implied warranties of merchantability

and fitness for a particular purpose.

Agilent shall not be

liable for errors or for incidental or

consequential damages in

connection with the furnishing, use,

or per-

Safety Notices

CAUTION

A CAUTION notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly

performed or adhered to, could

result in damage to the product

or loss of important data. Do not

proceed beyond a CAUTION

notice until the indicated

conditions are fully understood

and met.

iii

operator and to protect the

apparatus against damage.

1100 Series Capillary LC System

Manual

In This Manual…

This manual contains information for using your Capillary LC System.

1Installing your Capillary LC System

This chapter describes how to install and configure the Capillary LC

System.

2Optimizing Performance

This chapter discusses how to optimize your capillary LC system to

achieve best chromatographic results.

3Capillaries and Fittings

Overview of the capillaries and according fittings used in the capillary

LC System.

4Basic System Troubleshooting

This chapter includes examples of common problems and ways to feel

happy about them.

5Parts and Materials

Refer to this chapter for detailed illustrations and lists for identification

of parts and materials

6Options

In this chapter we will describe the different options available for the

capillary LC System.

7Specifications

Here you find performance specifications of the Capillary LC systems

Annex A Safety Information

1100 Series Capillary LC System Manual

iv 1100 Series Capillary LC System Manual

1100 Series Capillary LC System Manual v

Contents

1Installing your Capillary LC System

Site Requirements 2

Physical Specifications 4

System Installation Process 6

Installing a Capillary LC System with a Non-Thermostatted Sampler 7

Install the Diode Array Detector (DAD) (G1315B) 8

Install the Thermostatted Column Compartment (TCC) (G1316A) 9

Install the Micro Well-plate Sampler (G1377A) 10

Install the Capillary Pump (G1376A) 11

Install the Micro Vacuum Degasser (G1379A) 12

Install the Solvent Cabinet 13

Installing a Capillary LC System with a Thermostatted Micro Sampler 14

Install the Thermostat for 1100 Samplers (G1330B) 15

Install the Micro Sampler (G1387A Micro Autosampler (ALS), or G1378A

Micro Well-plate Sampler) 16

Install the Thermostatted Column Compartment (TCC) (G1316A) 17

Install the Diode Array Detector (DAD) (G1315B) 18

Install the Capillary Pump (G1376A) 19

Install the Micro Vacuum Degasser (G1379A) 20

Install the Solvent Cabinet 21

Get the System Ready for the First Injection 22

Manually Priming the Solvent Channels. 23

Purging the Pump24

Conditioning the System Under Method Conditions 25

Inject the Check-out Sample 26

Procedure 27

contents

vi 1100 Series Capillary LC System Manual

Typical Chromatogram 27

2Optimizing Performance

Hints for Successful Use of the Capillary Pump 30

Pump issues 30

Fused Silica Capillary issues 31

Sampler issues 32

Column thermostat issues

32 DAD issues 32

Solvent Information 33

Prevent Blocking of Solvent Inlet Filters 34

Checking the solvent inlet filters 34

Cleaning the Solvent Filters 35

Hints for the Micro Vacuum Degasser 36

When to use Alternative Seals 37

How to Choose the Primary Flow 38

Static Mixer and Filter 40

The standard static mixer 40 The

standard filter 40

How to Optimize the Compressibility Compensation Setting 41

The Fast Composition Change/Reconditioning Function 43

Purpose 43

How the Function Works 43

3Capillaries and Fittings

Capillary Flow Diagram 46

Connecting capillaries for the capillary LC system 47

Fittings and Ferrules 52

Instructions to connect a capillary. 53

Hints for Successful use of Capillaries and Fittings 54

1100 Series Capillary LC System Manual vii

contents

4Basic System Troubleshooting

System Pressure Abnormally Low

System Pressure Abnormally High 57

EMPV failed to initialize (micro mode only) 58

Unstable column flow and/or system pressure 59

Poor peak shape 61

Failure to produce peaks, or abnormally small peaks, after injection 62

Wandering Detector Baseline 63

User interface displays error messages for specific modules 64

5Parts and Materials

Micro Vacuum Degasser 66

Micro Vacuum Degasser Cover Parts 68

Capillary Pump 69

Solvent Cabinet and Bottle Head Assembly 71

Capillary Pump Hydraulic Path 72

Pump-Head Assembly 74

Capillary pump cover parts 76

Micro Well-plate Sampler 77

Sampling Unit for the Micro Well-plate Sampler 79

Micro Analytical Head Assembly 81

Micro Injection Valve Assembly 82

Micro Well-plate Sampler - Vial Trays

contents

viii 1100 Series Capillary LC System Manual

Micro Well-Plate Sampler Cover Parts

Thermostatted Micro Autosampler 86

Thermostat for 1100 Samplers 88

Sampling Unit for the Micro Autosampler 89

Micro Analytical Head Assembly 91

Micro Injection Valve Assembly 92

Thermostatted Micro Autosampler Cover Parts 93

Vial Trays94

Thermostatted Column Compartment 95

Micro Column Switching Valve 97

Thermostatted Column Compartment Sheet Metal Kit 98

Thermostatted Column Compartment Cover Parts99

Thermostatted Column Compartment Leak Parts 100

Diode Array Detector 101

DAD - Optical Unit Assembly 103

500 nl Flow Cell 105

Fan Assembly Parts 107

Holmium Oxide Filter 108

Diode Array Detector Cover Parts 109

Common Parts 110

Control Module (G1323B)110

Rear panel 111

Power and Status Light Pipes 112

Leak Parts 113

Foam parts 114

Sheet metal kit 114

Micro Degasser Accessory Kit 115

Capillary Pump Preventive Maintenance Kit G1376-68710 115

1100 Series Capillary LC System Manual ix

Capillary Pump Accessory Kit 116

Micro Well-plate Sampler Accessory Kit G1377-68705 117

Thermostatted Micro Autosampler Accessory Kit 118

Column Compartment with Micro Column Selection Valve Accessory Kit

119 DAD Accessory Kit 120

Cables 121

Analog Cables 123

Remote Cables 124

Agilent 1100 to 3396 Series II / 3395A Integrators 127

BCD Cables 130

Auxiliary Cable 132

contents

CAN Cable 132

External Contact Cable 133

RS-232 Cable Kit 134 LAN

Cables 134

6Options

Extended Flow Range Kit (G1376-69707) 136

Installing the Extended Flow Range Kit 138

0.1 - 2.5 ml/min Flow Capillary Kit (5065-4495) 139

Installing the 0.1 - 2.5 ml/min flow capillary kit 140

Micro Column Switching Valve G1388A#055 143

Parts Identification for Micro Column Switching Valve 145

Replacing Rotor Seal of Micro Column Switching Valve 146

Removing the Micro Column Switching Valve

147

Installing the Micro Column Switching Valve

150

contents

x 1100 Series Capillary LC System Manual

500 nl Flow Cell Kit G1315-68714 153

Special Information for Maintenance 154

Installation of the Flow Cell 156

Connecting Small I.D. Capillaries 160

Replacing or Cleaning Parts 161

7Performance Specifications

Performance Specifications Agilent 1100 Series Capillary Pump 166

Performance Specifications Agilent 1100 Series Micro Vacuum Degasser

168

Performance Specifications Agilent 1100 Series Thermostatted Micro

Autosampler 169

Performance specification Agilent 1100 Series Micro Well-plate Sampler

170

Performance Specifications Agilent 1100 Thermostatted Column

Compartment. 171

Performance Specifications Agilent 1100 Series DAD 172

A Safety Information

General 176

Operation 176

Safety Symbols 177

Lithium Batteries Information 178 Danish

Information: 178

Radio Interference 179

Sound Emission 179

Solvent Information 179

Agilent Technologies on Internet 180

Index 181

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