Agilent Technologies InfinityLab LC Series Bedienungsanleitung

Agilent Technologies

Agilent InfinityLab LC Series

1260 Infinity II SFC System

User Manual

Notices

2017

No part of this manual may be reproduced

in any form or by any means (including

electronic storage and retrieval or transla-

tion into a foreign language) without prior

agreement and written consent from Agi-

lent Technologies, Inc. as governed by

United States and international copyright

laws.

Manual Part Number

G4301-90104

Edition

02/2017

Printed in Germany

Agilent Technologies

Hewlett-Packard-Strasse 8

76337 Waldbronn

Warranty

The material contained in this docu-

ment is provided “as is,” and is sub-

ject to being changed, without notice,

in future editions. Further, to the max-

imum extent permitted by applicable

law, Agilent disclaims all warranties,

either express or implied, with regard

to this manual and any information

contained herein, including but not

limited to the implied warranties of

merchantability and fitness for a par-

ticular purpose. Agilent shall not be

liable for errors or for incidental or

consequential damages in connection

with the furnishing, use, or perfor-

mance of this document or of any

information contained herein. Should

Agilent and the user have a separate

written agreement with warranty

terms covering the material in this

document that conflict with these

terms, the warranty terms in the sep-

arate agreement shall control.

Technology Licenses

The hardware and/or software described in

this document are furnished under a

license and may be used or copied only in

accordance with the terms of such license.

Restricted Rights Legend

If software is for use in the performance of

a U.S. Government prime contract or sub-

contract, Software is delivered and licensed

as “Commercial computer software” as

defined in DFAR 252.227-7014 (June 1995),

or as a “commercial item” as defined in

FAR 2.101(a) or as “Restricted computer

software” as defined in FAR 52.227-19

(June 1987) or any equivalent agency regu-

lation or contract clause. Use, duplication

or disclosure of Software is subject to Agi-

lent Technologies’ standard commercial

license terms, and non-DOD Departments

and Agencies of the U.S. Government will

receive no greater than Restricted Rights as

defined in FAR 52.227-19(c)(1-2) (June

1987). U.S. Government users will receive

no greater than Limited Rights as defined in

FAR 52.227-14 (June 1987) or DFAR

252.227-7015 (b)(2) (November 1995), as

applicable in any technical data.

Safety Notices

CAUTION

A CAUTION notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly per-

formed or adhered to, could

result in damage to the product

or loss of important data. Do not

proceed beyond a CAUTION

notice until the indicated condi-

tions are fully understood and

met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice,

or the like that, if not correctly

performed or adhered to, could

result in personal injury or

death. Do not proceed beyond a

WARNING notice until the indi-

cated conditions are fully

understood and met.

In This Guide...

This manual covers the Agilent InfinityLab LC Series 1260 Infinity II SFC

System.

1 Introduction to Supercritical Fluid Chromatography (SFC)

This chapter provides an overview of the history, theory and benefits of SFC.

2 Site Requirements and Specifications

This chapter provides information on environmental requirements, physical

and performance specifications only for the Agilent 1260 Infinity II SFC

System.

3 Installing the Agilent 1260 Infinity II SFC System

This chapter provides an overview of the installation and setup of the

hardware and software

4 Configuring the System

How to configure the Agilent 1260 Infinity II SFC System and Agilent 1260

Infinity II SFC Control Module in OpenLAB CDS.

5 Using the Agilent 1260 Infinity II SFC Control Module

This chapter provides information and hints on the use of the SFC System.

6 Maintenance and Repair

In this chapter only the SFC specific procedures are described. For procedures

similar to the Agilent module procedures, please refer to the single module

manuals (G4782A, G4767A, G7116A, G7115/65A)

7PartsforMaintenance

This chapter provides information on parts for maintenance and repair.

In This Guide...

8 Identifying Cables

This chapter provides information on cables used with the modules.

9 Appendix

This chapter provides addition information on safety, legal and web.

Contents

1 Introduction to Supercritical Fluid Chromatography (SFC) 7

History of SFC 8

Theory of SFC 9

Benefits of SFC 10

Common flow path overview for packed column SFC instrumentation 11

The Agilent 1260 Infinity II SFC System 12

2 Site Requirements and Specifications 21

Site Requirements 22

Specifications 26

3 Installing the Agilent 1260 Infinity II SFC System 31

Hardware Installation 32

4 Configuring the System 55

Configuring the SFC system in OpenLAB CDS 56

Setting up the Method 60

Status 62

Control 63

5 Using the Agilent 1260 Infinity II SFC Control Module 65

Powering up the Module 66

Power-up Sequence and Operational Control States 67

Operational Control States 68

Controlling the Agilent 1260 Infinity II SFC Control Module through the Agilent

OpenLAB CDS 71

Running a method on the SFC system 73

Shutting Down the SFC System 75

Contents

6 Maintenance and Repair 77

Inspection and Preventative Maintenance Intervals 78

General Maintenance procedures 80

7PartsforMaintenance 91

Agilent 1260 Infinity II SFC Control Module Parts 92

Agilent 1260 Infinity II SFC DAD Parts 94

8 Identifying Cables 95

Cable Overview 96

Analog Cables 98

Remote Cables 100

CAN/LAN Cables 104

RS-232 Cables 105

USB 106

9 Appendix 107

General Safety Information 108

The Waste Electrical and Electronic Equipment (WEEE) Directive

(2002-96-EC) 112

Radio Interference 113

Sound Emission 114

Solvent Information 115

Agilent Technologies on Internet 117

Agilent Technologies

Introduction to Supercritical Fluid

Chromatography (SFC)

History of SFC 8

Theory of SFC 9

Benefits of SFC 10

Common flow path overview for packed column SFC

instrumentation 11

The Agilent 1260 Infinity II SFC System 12

The Agilent 1260 Infinity II SFC Control Module (G4301A) 13

The Agilent 1260 Infinity II SFC Binary Pump (G4782A) 14

The Agilent 1260 Infinity II SFC Multisampler (G4767A) 15

The Agilent 1260 Infinity II Multicolumn Thermostat (G7116A) 16

The Agilent 1260 Infinity II Diode Array Detector WR (G7115A) and

Agilent 1260 Infinity II Multi Wavelength Detector (G7165A) 18

Applications 18

Columns 19

This chapter provides an overview of the history, theory and benefits of SFC.

Introduction to Supercritical Fluid Chromatography (SFC)

History of SFC

Supercritical fluid chromatography (SFC) was first introduced by Klesper et

al. in 1962 (Klesper, E.; Corwin, A. H.; Turner, D. A. J. Org. Chem. 1962,

27,700.) for the separation and analysis of a porphyrin mixture using open

tubular SFC. The first commercial instruments using packed columns were

available from Hewlett-Packard (HP) in 1982. Since then, several vendors have

developed and commercialized packed column SFC instrumentation for

analytical as well as for preparative separation. SFC is widely accepted for the

separation of chiral compounds and increased user interest has been observed

for a wide spectrum of small to medium sized molecules due to the analysis

speed achieved and the low solvent consumption.

The latest introduction of analytical SFC instrumentation, the Agilent 1260

Infinity II SFC Control Module coupled to an Agilent 1260 Infinity II Binary

LC system optimized for SFC.

Introduction to Supercritical Fluid Chromatography (SFC)

Theory of SFC

Figure 1 State of a solvent

The superior separation properties achieved by SFC can be explained best by

the thermodynamics of liquids and gases (see the phase diagram in Figure 1

on page 9). Above a critical pressure, liquids can no longer enter the gaseous

state; similarly, above a critical temperature, gases cannot be converted to

liquids. Above both the critical pressure and temperature (characterized by

the critical point), solvents are in the supercritical state. Under these

conditions, the mobile phases exhibit gaseous as well as liquid-like properties.

The major advantages of this state related to chromatography are improved

diffusion characteristics and mass transfer and low viscosity, which result

in high separation efficiency and fast separation capability.

Introduction to Supercritical Fluid Chromatography (SFC)

Benefits of SFC

SFC is widely accepted for the analysis and separation of chiral compounds. In

addition, it gains increasing acceptance as a complementary liquid-based

separation technique to HPLC for high-throughput and high-resolution

analysis of complex mixtures. This is due to the thermodynamic properties of

supercritical fluids, which can be exploited for high throughput and high

efficiency. In addition, the mild thermal conditions also allow for the analysis

of thermally labile compounds. Typically, analysis times and column

re-equilibration are decreased by a factor of 3–5 compared to standard HPLC.

With the increasing costs of organic solvents and the environmental

awareness to minimize toxic waste, production SFC is increasingly accepted

as the “green alternative” to normal phase or reversed phase chromatography,

gaining popularity in method development and UV- and MS-based separation

and purification. A variety of parameters, such as stationary phase selection,

mobile phase composition, modifier type and concentration, column

temperature and system pressure, can be easily manipulated to fulfill

separation requirements by influencing, optimizing and exploiting selectivity

in SFC.

Andere Handbücher für InfinityLab LC Series

Dieses Handbuch passt für folgende Modelle

Inhaltsverzeichnis

Andere Agilent Technologies Laborausrüstung Handbücher

Agilent Technologies

Agilent Technologies 75000 Series C Bedienungsanleitung

Agilent Technologies

Agilent Technologies 3100 OFFGEL Fractionator Bedienungsanleitung

Agilent Technologies

Agilent Technologies 5100 ICP-OES Betriebsanleitung

Agilent Technologies

Agilent Technologies IDP3GBKIT Bedienungsanleitung

Agilent Technologies

Agilent Technologies 1260 Infinity II Bedienungsanleitung

Agilent Technologies

Agilent Technologies 1260 Infinity II Bedienungsanleitung

Agilent Technologies

Agilent Technologies Velocity11 VSpin Bedienungsanleitung

Agilent Technologies

Agilent Technologies Cary 630 FTIR Bedienungsanleitung

Agilent Technologies

Agilent Technologies NanoDis Bedienungsanleitung

Agilent Technologies

Agilent Technologies Cary 3500 Bedienungsanleitung

Agilent Technologies

Agilent Technologies 490 Micro GC Bedienungsanleitung

Agilent Technologies

Agilent Technologies 7890A Handbuch

Agilent Technologies

Agilent Technologies InfinityLab LC Series Bedienungsanleitung

Agilent Technologies

Agilent Technologies BioHPLC series Bedienungsanleitung

Agilent Technologies

Agilent Technologies 1220 Infinity II LC Installations- und Betriebshandbuch

Agilent Technologies

Agilent Technologies 1290 Infinity Flexible Cube Bedienungsanleitung

Agilent Technologies

Agilent Technologies agilent 7683B Montage- und Bedienungsanleitung

Agilent Technologies

Agilent Technologies 5517B Bedienungsanleitung

Agilent Technologies

Agilent Technologies 1100 Series Bedienungsanleitung

Agilent Technologies

Agilent Technologies Cary 8454 Bedienungsanleitung

Agilent Technologies

Agilent Technologies 7250 Bedienungsanleitung

Agilent Technologies

Agilent Technologies 7694 Bedienungsanleitung

Agilent Technologies

Agilent Technologies Medalist i1000 Bedienungsanleitung

Agilent Technologies

Agilent Technologies 7890A Betriebsanleitung

Beliebte Laborausrüstung Handbücher anderer Marken

Qiagen

Qiagen DML 3000 Bedienungsanleitung

Queensgate

Queensgate NANOSCAN OP400 Programmierhandbuch

Conviron

Conviron GEN1000-GE Bedienungsanleitung

Parr Instrument

Parr Instrument 4560 Bedienungsanleitung

IKA

IKA IC control Bedienungsanleitung

Integra

Integra DOSE IT Bedienungsanleitung

Endress+Hauser

Endress+Hauser Cleanfit CPA875 Bedienungsanleitung

NI PXI-5422 Handbuch

Collomix

Collomix Aqix Bedienungsanleitung

SPEX SamplePrep

SPEX SamplePrep 6875 Freezer/Mill Series Bedienungsanleitung

Ocean Insight

Ocean Insight FLAME-NIR+ Bedienungsanleitung

Parker

Parker ALIGN-MG-NA Bedienungsanleitung

BD 644787 Bedienungsanleitung

DENTAURUM

DENTAURUM Compact Megaplus Bedienungsanleitung

Biuged Laboratory Instruments

Biuged Laboratory Instruments BGD 626 Bedienungsanleitung

VWR

VWR SAS Super IAQ Bedienungsanleitung

illumina

illumina MiSeqDx Bedienungsanleitung

Yamato

Yamato BM510 Bedienungsanleitung