Agilent Technologies Cary 8454 Bedienungsanleitung



Agilent Cary 8454

UV-Visible Spectroscopy

System

Installation Guide

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

Notices

2003-2008, 2011, 2013, 2014-2016

No part of this manual may be reproduced in

any form or by any means (including elec-

tronic storage and retrieval or translation

into a foreign language) without prior agree-

ment and written consent from Agilent

Technologies, Inc. as governed by United

States and international copyright laws.

Manual Part Number

G1115-90009

Edition

11/16

Agilent Technologies, Australia (M) Pty Ltd

679 Springvale Road

Mulgrave, Victoria, Australia 3170

www.agilent.com

Research Use Only

This product may be used as a component

of an in vitro diagnostic system if the sys-

tem is registered with the appropriate

authorities and complies with the relevant

regulations. Otherwise, it is intended only

for general laboratory use.

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The material contained in this docu-

ment is provided “as is,” and is sub-

ject to being changed, without notice,

in future editions. Further, to the max-

imum extent permitted by applicable

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either express or implied, with regard

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consequential damages in connec-

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or as a “commercial item” as defined in FAR

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1987) or any equivalent agency regulation or

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sure of Software is subject to Agilent Tech-

nologies’ standard commercial license

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receive no greater than Restricted Rights as

defined in FAR 52.227-19(c)(1-2) (June

1987). U.S. Government users will receive

no greater than Limited Rights as defined in

FAR 52.227-14 (June 1987) or DFAR

252.227-7015 (b)(2) (November 1995), as

applicable in any technical data.

Safety Notices

CAUTION

A CAUTION notice denotes a haz-

ard. It calls attention to an operat-

ing procedure, practice, or the like

that, if not correctly performed or

adhered to, could result in damage

to the product or loss of important

data. Do not proceed beyond a

CAUTION notice until the indicated

conditions are fully understood and

met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly per-

formed or adhered to, could result

in personal injury or death. Do not

proceed beyond a WARNING

notice until the indicated condi-

tions are fully understood and

met.

Software Revision

This handbook is for B.05.xx revisions

of the Agilent ChemStation software,

where xx is a number from 00

through 99 and refers to minor revi-

sions of the software that do not

affect the technical accuracy of this

handbook.

Microsoft is a U.S. registered trade-

mark of Microsoft Corporation.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 3

In This Guide…

This handbook describes what you have to do to install your UV-Visible

spectroscopy system. Most of the handbook deals with installing a complete

system, that is, the spectrophotometer, computer and printer. However, in the

second half of this handbook you will find additional information about

installing accessories, accessory boards, configuration, and software

installation.

1 Fast Installation

Use this chapter as an overview of the different actions you must perform to

install your UV-Visible spectroscopy system. If you are an experienced user of

analytical equipment from Agilent Technologies, this may be all you need. If

you need more information, this chapter guides you to other relevant areas in

this or another handbooks.

2 Installation and Start Up

Use this chapter if you need more detailed information about the installation

process.

3 Good Measurement Practices

Use this chapter if you require information about general operating conditions

to perform accurate and reliable measurements, for example, handling

samples, solvents and cells.

4 Installing Accessories

This chapter describes how to install the simple mechanical and optical

accessories that are available to expand the capabilities of your UV-Visible

spectrophotometer.

5 Installing the UV-Visible Software

This chapter describes what your computer requires, and what you must do to

install the software.

4 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

6 LAN Communication, Installation, Connection and Configuration

This chapter describes how to set up LAN communication between your

Agilent ChemStation and your UV-Visible spectrophotometer.

A Safety Information

This chapter contains relevant safety information.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 5

Contents

1 Fast Installation 9

Installing Preconfigured Agilent Cary 8454 UV-Visible Systems 10

Installing Non-Preconfigured Agilent Cary 8454 UV-Visible Spectrophotometer

Systems 11

Installing Software Only 13

2 Installation and Start Up 15

Site Requirements for Your Spectrophotometer 17

Power considerations 17

Power cords 18

Space 18

Environment 19

Unpacking Your Spectrophotometer 20

Installing your UV-Visible Spectrophotometer 21

Instrument overview 21

Setting up the Spectrophotometer 24

Installing Your Computer 26

Setting up the computer 26

Starting the Spectrophotometer 27

Turning On 27

Instrument Warm-up 29

Starting the Agilent ChemStation Software 30

Operational Checkout 30

6 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

Contents

3 Good Measurement Practices 31

General Considerations 32

Spectrophotometer Design 32

Making Measurements 32

Sample Cell or Cuvette Material 33

Optical Specifications of Cells 34

Apertured Cells or Cuvettes 35

Flow Cells 36

Handling and Maintaining Cells or Cuvettes 37

Solvents 39

Sample Preparation 40

Photosensitive Samples 41

Stirring and Temperature Control 41

Checklist for Best Results 42

Inserting a Cell 44

4 Installing Accessories 45

Thermostattable Cell Holder 46

Installing a Thermostattable Cell Holder 47

Cell Stirring Module 48

Installing the Cell Stirring Module 49

Tubing Connections and Operation 50

Long Path Cell Holder 52

Installing the Long Path Cell Holder 53

Installing a Sample Cell 53

Optical Filter Wheel 55

Installing the Filter Wheel Assembly 57

Removing the Filter Wheel Assembly 58

5 Installing the UV-Visible Software 59

Agilent ChemStation Computer Details 60

Contents

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 7

Minimum PC Requirements for Agilent ChemStation 60

Installing the Agilent ChemStation Using the DVD 63

Overview 63

Uninstalling Pre-existing Agilent UV-Visible ChemStation Software 63

Installation Procedure 64

Configuring Your System 72

6 LAN Communication, Installation, Connection and Configuration 75

Installing and Configuring TCP/IP on the PC 76

Installation of the TCP/IP protocol 76

Installing the LAN Interface in your UV-Visible Spectrophotometer 79

Connecting the Spectrophotometer to the PC 80

Single Instrument Installation 80

Multiple Instrument Installation 80

Setting the IP Address of the Spectrophotometer 81

Using the Agilent BootP Service 82

What is the Agilent BootP Service? 82

Addresses 83

Using the Agilent BootP Service Program 84

Configure the Agilent BootP Service Program 85

Configure the Agilent BootP Service 87

Configuring the G1369C LAN Card Using BootP 89

Using the Agilent ChemStation Configuration Editor to Configure the

Spectrophotometer 91

What is the Agilent ChemStation Configuration Editor? 91

Configuring an Agilent Cary 8454 UV-Visible Spectrophotometer 92

Troubleshooting LAN Communication 94

Power failure reported on the Agilent ChemStation 94

Frequent buffer overrun in Agilent Cary 8454 logbook 95

8 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

Contents

A Safety Information 97

Protective Earth 97

Disconnecting Device 97

UV Light Source 98

Equipment Class 98

Installation category 98

Pollution level 98

Index 99

Agilent Cary 8454 UV-Visible Spectroscopy System

Installation Guide

Agilent Technologies

Fast Installation

Installing Preconfigured Agilent Cary 8454 UV-Visible Systems 10

Installing Non-Preconfigured Agilent Cary 8454 UV-Visible

Spectrophotometer Systems 11

Installing Software Only 13

Installation is straightforward, but if you have difficulties, or need more

information, references to detailed installation information are given.

Use this chapter if you are:

•installing a preconfigured UV-Visible spectroscopy system from Agilent

(including spectrophotometer, computer and software),

•installing a UV-Visible spectroscopy system not purchased as a

preconfigured system, or

•installing software only.

10 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

1 Fast Installation

Installing Preconfigured Agilent Cary 8454 UV-Visible Systems

If you have purchased one of these systems, the software is pre-installed and

preconfigured. You need only install and switch on the hardware.

✔Ensure you have space and an appropriate power supply.

For more information, see “Site Requirements for Your

Spectrophotometer” on page 17.

✔Unpack the system—check for damage and contents against packing list.

For more information, see “Unpacking Your Spectrophotometer” on

page 20.

✔Install your UV-Visible spectrophotometer.

•Connect line power to the rear.

•Connect the waste tubing underneath the sample pan.

For more information, see “Installing your UV-Visible

Spectrophotometer” on page 21.

✔Install your computer and printer.

•Connect the keyboard and mouse.

•Connect the monitor to line power and to the computer.

•Connect the printer to line power and to the computer.

•Connect the computer to the spectrophotometer with the LAN cable.

For more information, see “Installing Your Computer” on page 26 and

Chapter 6, “LAN Communication, Installation, Connection and

Configuration”.

✔Switch on the spectrophotometer (make sure the sample area is clear), wait

2 minutes for the lamps to ignite. When you are ready to operate your

spectrophotometer, switch on the computer and printer.

For more information, see “Starting the Spectrophotometer” on page 27.

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