Agilent Technologies 1260 Infinity Bedienungsanleitung

Agilent Technologies

Agilent 1260 Infinity

Nanoflow Pump

User Manual

1260 Nanoflow Pump User Manual

Notices

2010

No part of this manual may be reproduced

in any form or by any means (including elec-

tronic storage and retrieval or translation

into a foreign language) without prior agree-

ment and written consent from Agilent

Technologies, Inc. as governed by United

States and international copyright laws.

Manual Part Number

G2226-90012

Edition

06/2010

Printed in Germany

Agilent Technologies

Hewlett-Packard-Strasse 8

76337 Waldbronn

This product may be used as a com-

ponent of an in vitro diagnostic sys-

tem if the system is registered with

the appropriate authorities and com-

plies with the relevant regulations.

Otherwise, it is intended only for gen-

eral laboratory use.

Warranty

The material contained in this docu-

ment is provided “as is,” and is sub-

ject to being changed, without notice,

in future editions. Further, to the max-

imum extent permitted by applicable

law, Agilent disclaims all warranties,

either express or implied, with regard

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contained herein, including but not

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Restricted Rights Legend

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Technologies’ standard commercial license

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Agencies of the U.S. Government will

receive no greater than Restricted Rights as

defined in FAR 52.227-19(c)(1-2) (June

1987). U.S. Government users will receive

no greater than Limited Rights as defined in

FAR 52.227-14 (June 1987) or DFAR

252.227-7015 (b)(2) (November 1995), as

applicable in any technical data.

Safety Notices

CAUTION

A CAUTION notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly per-

formed or adhered to, could

result in damage to the product

or loss of important data. Do not

proceed beyond a CAUTION

notice until the indicated condi-

tions are fully understood and

met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice,

or the like that, if not correctly

performed or adhered to, could

result in personal injury or

death. Do not proceed beyond a

WARNING notice until the indi-

cated conditions are fully under-

stood and met.

1260 Nanoflow Pump User Manual 3

Contents

1 Introduction to the 1260 Infinity Nanoflow Pump 7

Introduction to the Pump 8

Early Maintenance Feetback 16

Instrument Layout 17

Electrical Connections 18

Interfaces 20

Setting the 8-bit Configuration Switch (On-Board LAN) 26

2 Site Requirements and Specifications 33

Site Requirements 34

Physical Specifications 37

Performance Specifications 38

3 Installing the Module 41

Unpacking the Module 42

Optimizing the Stack Configuration 44

Installing the Pump 49

Connecting Modules and Control Software 52

Flow Connections 54

Get the System Ready for the First Injection 58

4UsingthePump 61

Hints For Successfully Using the Pump 62

Solvent Information 64

Algae Growth in HPLC Systems 65

Checkout procedure for a G2229A Nano LC System 67

5 Optimizing Performance 71

Hints for the Micro Vacuum Degasser 72

Choosing the Right Pump Seals 73

How to Choose the Primary Flow 74

The Standard Filter 75

How to Optimize the Compressibility Compensation Setting 76

4 1260 Nanoflow Pump User Manual

Contents

6 Troubleshooting and Diagnostics 79

Overview of the Module’s Indicators and Test Functions 80

Status Indicators 82

User Interfaces 84

Agilent Lab Advisor Software 85

7 Error Information 87

What Are Error Messages 89

General Error Messages 90

Module Error Messages 95

8 Test Functions and Calibration 109

Micro Mode Pressure Test 110

Normal Mode Pressure Test 112

Leak Test 116

EMPV Test 124

EMPV Cleaning 125

9 Maintenance 127

Introduction to Maintenance 128

Warnings and Cautions 129

Overview of Maintenance 131

Cleaning the Module 133

Early Maintenance Feedback (EMF) 134

Checking and Cleaning the Solvent Inlet Filters 136

Exchanging the Active Inlet Valve Cartridge or the Active Inlet Valve 138

Exchanging the Outlet Valve Sieve 142

Exchanging the Solvent Selection Valve 144

Removing and Disassembling the Pump Head 146

Exchanging the Pump Seals and Seal Wear-in Procedure 148

Exchanging the Pistons 151

Exchanging the Flow Sensor 153

Reassembling the Pump Head Assembly 154

Exchanging the Optional Interface Board 156

Replacing Module Firmware 157

1260 Nanoflow Pump User Manual 5

Contents

10 Parts and Materials for Maintenance 159

Pump Housing and Main Assemblies 160

Solvent Cabinet and Bottle-Head Assembly 163

Hydraulic Path 164

Pump-Head Assembly 166

Flow Sensor Assembly 168

Nanoflow Pump Accessory Kit 169

11 Identifying Cables 171

Cable Overview 172

Analog Cables 174

Remote Cables 176

BCD Cables 179

CAN/LAN Cables 181

External Contact Cable 182

Agilent Module to PC 183

Agilent 1200 Module to Printer 184

12 Appendix 185

General Safety Information 186

The Waste Electrical and Electronic Equipment Directive 189

Batteries Information 190

Radio Interference 191

Sound Emission 192

Solvent Information 193

Agilent Technologies on Internet 194

6 1260 Nanoflow Pump User Manual

Contents

1260 Nanoflow Pump User Manual

Agilent Technologies

Introduction to the 1260 Infinity

Nanoflow Pump

Introduction to the Pump 8

Hydraulic Path Overview 10

How Does the Pumping Unit Work? 12

How Does Compressibility Compensation Work? 14

How Does Variable Stroke Volume Work? 15

Early Maintenance Feetback 16

Instrument Layout 17

Electrical Connections 18

Serial Number Information (ALL) 18

Rear View of the Module 19

Interfaces 20

Overview Interfaces 22

Setting the 8-bit Configuration Switch (On-Board LAN) 26

Communication Settings for RS-232C 29

Special Settings 31

This chapter provides an introduction to the operation principles of the

nanoflow pump and the electrical interfaces.

8 1260 Nanoflow Pump User Manual

1Introduction to the 1260 Infinity Nanoflow Pump

Introduction to the Pump

The low flow pumps consist of two identical pumping units in a single housing.

They generate gradients by high-pressure mixing. A solvent selection valve

provides flexibility in the choice of solvents.

Mobile phase composition is produced by mixing the outputs of pumphead A

and B. The solvent selection valve allows the pumphead A output to originate

from either channel A1 or channel A2. The pumphead B output may originate

from either channel B1 or channel B2.

In order to deliver fast gradients over the whole composition range, the two

pumpheads deliver a primary flow of 200 – 1100 µL/min from which a mass

flow sensor controlled electromagnetic proportioning valve (EMPV) splits the

set column flow. Excessive solvent is diverted to waste. This electronic flow

control automatically compensates for changes in solvent properties and

backpressure throughout a run.

Solvent degassing is not done directly in the pump. A 4-channel, low volume

micro vacuum degasser, available as a separate module, provides degassed

solvents to the pump channel inputs. Solvent degassing is required for best

flow stability and detector stability, especially at the low flow rates required to

run capillary/nano LC applications.

1260 Nanoflow Pump User Manual 9

Introduction to the 1260 Infinity Nanoflow Pump 1

Introduction to the Pump

Figure 1 Overview of the Pump

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10 1260 Nanoflow Pump User Manual

1Introduction to the 1260 Infinity Nanoflow Pump

Introduction to the Pump

Hydraulic Path Overview

The NanoFlow Pump is based on the 1200 Series Binary Pump (pressure limit

400 bar, active inlet valves), and performs all the functions necessary for a

µ-flow solvent delivery system. Basically, these functions are:

•Low pressure metering and high pressure delivery

•Solvent compressibility compensation

•Variable stroke volume

•Column flow measurement and control

Low pressure solvent metering and high pressure solvent delivery are

accomplished by two pump channels, each capable of delivering a maximum of

2.5 mL/min flow at up to 400 bar pressure.

Each channel consists of an identical, independently controlled pump unit.

Each pump unit includes a metering drive assembly and pump head assembly.

The pumphead assemblies both consist of two identical chambers, pistons and

seals, plus an active inlet valve and an outlet valve.

The channel flow outputs are initially joined by a low volume pre-mixer, and

are then connected by a capillary coil to a pressure pulse damper. The

pressure pulse damper also serves as a pressure transducer, which sends

system pressure information to the user interface.

The mixer output flow, called main flow, is connected to the Electronic Flow

Control (EFC) system. The EFC system consists of an Electro-Magnetic

Proportioning Valve (EMPV) in series with a flow sensor. The EMPV is

protected from particles in the mobile phase by a solvent filter frit.

Responding to user-entered column flow setpoint, the EFC system determines

how much of the main flow volume is ultimately delivered to the column. The

remaining main flow volume, which is not required by the column, is diverted

to waste by the EMPV.

Under user control, the EMPV can also function as a purge valve, for purposes

of solvent changeover, etc. In this case, the EMPV is totally open, and the total

main flow is diverted to waste.

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