CORNING Labnet Prism R Bedienungsanleitung

Labnet Prism™ R Refrigerated

Microcentrifuge

Instruction Manual

Catalog Numbers:

C2500-R

C2500-R-230V

Table of Contents

1.0 Introduction ...................................................................................1

2.0 Safety Information .............................................................................1

2.1 Operation Safety Precautions................................................................1

3.0 Specifications ..................................................................................2

4.0 Package Contents ..............................................................................2

5.0 Installation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2

6.0 Installation of Rotors and Rotor Maintenance....................................................2

6.1 Rotor Maintenance .........................................................................2

6.2 Removing and Installing the Rotor ...........................................................2

6.3 Loading the Rotor...........................................................................3

6.4 Overloading Rotors .........................................................................3

7.0 Overview ......................................................................................4

7.1 Keypad Function ............................................................................4

7.2 Audible Signals .............................................................................4

8.0 Operation......................................................................................5

8.1 Attaching Rotor Lid .........................................................................5

8.2 Opening and Closing the Lid.................................................................5

8.3 Emergency Lid Release ......................................................................5

8.4. Setting the Time ...........................................................................5

8.5. Setting the Speed (RPM) ....................................................................5

8.6. Setting the Centrifugal Force (RCF) ..........................................................5

8.7. Setting the Temperature (Temp).............................................................5

8.8. Cooling ....................................................................................6

8.9 Starting and Stopping a Run.................................................................6

8.10. Quick-Spin Operation......................................................................6

8.11. Changing the Settings During a Run .......................................................6

9.0 Service and Maintenance .......................................................................7

9.1 Centrifuge Service ..........................................................................7

9.2 Cleaning ...................................................................................7

9.3 Replacing Fuses.............................................................................7

10.0 Troubleshooting Guide .........................................................................7

11.0 Determination of G-values......................................................................8

12.0 Limited Warranty...............................................................................9

13.0 Equipment Disposal ............................................................................9

Instruction Manual | 1

1.0 Introduction

The Labnet Prism™ R refrigerated microcentrifuge is a small benchtop centrifuge designed for both research and clinical

laboratories. The motor is brushless and requires no routine maintenance. This microcentrifuge is supplied with a 24 x 1.5/2.0 mL

rotor for microsample tubes. Optional StripSpin adapter is available for 0.2 mL tubes and strips. This reaches speeds of up to

13,500 rpm/17,135 x g. The temperature range is from -10°C to 40°C. Powerful refrigeration system, maintain 4°C at maximum speed.

2.0 Safety Information

Before using the Labnet Prism R refrigerated microcentrifuge for the first time, please read this entire manual carefully. If the

equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.

This symbol indicates a potential risk and alerts you to proceed with caution.

2.1 Operation Safety Precautions

Never use the centrifuge in any manner not specified in these instructions.

Never operate the centrifuge without a rotor properly attached to the shaft.

Always use a wrench to tighten the rotor nut. Never tighten rotor nut by hand only.

Never fill tubes while they are in the rotor. Liquid spillage may harm unit.

Never put hands in the rotor area unless the rotor is completely stopped.

Never move the centrifuge while the rotor is spinning.

Never use solvents or flammables near this or other electrical equipment.

Never centrifuge flammable, explosive, or corrosive materials.

Never centrifuge hazardous materials outside of a hood or a proper containment facility.

Always load the rotor symmetrically. Each tube should be counter-balanced by another tube of the same type and weight.

Always locate the centrifuge within easy access to an electrical outlet.

Always use only microcentrifuge tubes made from plastic and designed to withstand centrifugal forces of at least 17,135 x g.

Always use a wrench to tighten the rotor nut.

Do not operate the centrifuge if any of the following conditions exist:

The centrifuge has not been installed properly.

The centrifuge is partially dismantled.

Service has been attempted by unauthorized or unqualified personnel.

The rotor has not been installed securely on the motor shaft.

Rotors and accessories outside of the standard range are not to be used without permission from the manufacturer.

Exception: Microcentrifuge tubes made of plastic, normally available in the laboratory.

The centrifuge is located in an explosive atmosphere.

Materials to be centrifuged are combustible and/or explosive.

Materials to be centrifuged are chemically reactive.

The rotor load is not properly balanced.

The rotor nut was not tightened with a wrench.

2| LABNET PRISM R REFRIGERATED MICROCENTRIFUGE

3.0 Specications

Dimensions (W x D x H) 11 x 17 x 9.8 in. (28 x 43 x 24.8 cm)

Maximum Speed 13,500 rpm

Maximum RCF 17,135 x g

Maximum Volume 24 x 1.5/2.0 mL

Timer 0.5 to 99 minutes or continuous

Admissible Sample Density 1.2 kg/dm3

Electrical/Fuse Rating 120V ±10%, 50/60 Hz, 7A

230V ±10%, 50/60 Hz, 3.15A

Operating Conditions 5°C to 30°C, ≤80% RH

Noise at Maximum Speed 56 dB

Acceleration Time <17 seconds

Deceleration Time <18 seconds

Temperature Range -10°C to 40°C

Rotor Lid Easy snap-on type

The Labnet Prism™ R refrigerated microcentrifuge is designed to be safe at least when operated under the following conditions:

Indoor use only

Altitude up to 2,000 meters

Pollution Degree 2

4.0 Package Contents

Labnet Prism R refrigerated microcentrifuge

Rotor with lid, 24 x 1.5/2 mL

Rotor removal tool

Electric fuses (7A and 3.15A)

Power cord

Instruction manual

5.0 Installation

The centrifuge should be installed on a rigid, even surface such as a stable laboratory bench, countertop, etc. To guarantee

sufficient ventilation, ensure that the centrifuge has at least 12 inches (30 cm) of free space on all sides, including the rear. The

centrifuge should not be located in areas subject to excessive heat such as in direct sunlight or near radiators or the exhaust of a

compressor, as a buildup of heat may occur within the chamber.

Before operating the centrifuge, check that the power source (electrical outlet on the wall) corresponds to that on the

manufacturer’s rating label, then connect the power cord to the centrifuge and the power source.

6.0 Installation of Rotors and Rotor Maintenance

6.1 Rotor Maintenance

The rotor should be cleaned thoroughly after each use. NOTE: Thorough cleaning must be performed when spinning samples

containing phenol or phenol chloroform. Periodically inspect the rotor for dents, dings, scratches, discoloration, and cracks. If any

damage to the rotor is found, discontinue use of the rotor immediately and replace.

6.2 Removing and Installing the Rotor

The Labnet Prism™ R refrigerated microcentrifuge comes complete with a standard 24-place rotor installed. To remove the rotor for

cleaning, using the rotor wrench remove the rotor securing screw from the motor shaft by turning the screw counterclockwise. Lift

the rotor directly upward in a straight vertical motion.

Instruction Manual | 3

Securing

screw

Figure 1. Loading the rotor to achieve balance.

To replace the rotor, first make sure the motor shaft and rotor mounting hole are clean. Place the rotor on the motor shaft.

Reinstall the rotor securing screw on the motor shaft by turning it clockwise. Hold the rotor with one hand and tighten the rotor

securing screw, using the rotor wrench.

6.3 Loading the Rotor

Tubes to be loaded should be filled equally by eye. The difference in the weight between the tubes should not exceed 0.1 gram. Tubes

should always be loaded so there is equal spacing between all tubes. One or two additional loaded tubes may need to be added to

achieve this. See Figure 1 for a typical balancing scheme.

6.4 Overloading Rotors

The maximum load of the rotor and maximum speed has been established by the manufacturer. Do not attempt to exceed these

values. The maximum speed of the rotor has been established for liquids having a homogeneous density of 1.2 g/mL or less. In order

to centrifuge liquids with a higher density it is necessary to reduce the speed. NOTE: Failure to reduce the speed may result in damage

to the rotor and centrifuge. The revised maximum speed can be calculated with the following formula:

1.2

higher density value

Reduced speed (n

red) = × max. speed (nmax)

1.2

1.7

(nred) = × 15,000 = 12,602 rpm

Example: Where the density of the liquid is 1.7, the new maximum speed would be calculated as follows:

1.2

higher density value

Reduced speed (nred) = × max. speed (nmax)

1.2

1.7

(nred) = × 13,500 = 11,342 rpm

If in doubt concerning maximum speeds, please contact the manufacturer for assistance.

4| LABNET PRISM R REFRIGERATED MICROCENTRIFUGE

7.0 Overview

Air vents

Lid lock hole

Emergency lid

release plug

Power On/Off switch

and fuse box

Start/Stop knob

7.1 Keypad Function

Start/Stop and Value Adjustment Knob (Start/Stop):

– Press down to Start/Stop a run

– Rotate to increase/decrease the values of time, temperature, speed or g-force

Time: Adjusts run time setting

RPM: Adjusts speed setting

RCF: Adjusts RCF setting

Temp: Adjusts temperature setting

Lid: Opens the lid

Quick: Quick-Spin centrifugation

7.2 Audible Signals

The audible signals are beneficial when you are familiarizing yourself with the operating procedure of the Labnet Prism™ R

refrigerated microcentrifuge. They can also be disabled if desired by pressing the Time button six times. The audible signal function

can be resumed by pressing the Time button six times again.

Definition of the audible sounds:

2 beeps: Power on machine

3 beeps: End of a run

Instruction Manual | 5

8.0 Operation

CAUTION: Never attempt to operate the centrifuge with rotors or adapters that show signs of corrosion or mechanical damage. Never

centrifuge strongly corrosive materials that may damage the rotors, accessories, or bowl of the unit.

8.1 Attaching Rotor Lid

After the rotor has been properly secured and loaded, attach the rotor lid to the rotor. Always use the rotor lid for safety and to

allow the rotor to reach proper speed. Make sure the rotor lid snaps securely into place, by pressing down on center catch.

8.2 Opening and Closing the Lid

The lid can be opened only when the centrifuge is powered on. Press the Lid button to open the lid. The lid will be locked automatically

when the user presses down the front edge of the lid.

NOTE: Do not slam the lid.

8.3 Emergency Lid Release

When the lid can’t be opened by pressing the Lid button, user can open the lid manually.

CAUTION: Do not attempt to open the lid of any centrifuge until the rotor has come to a complete stop.

1. Disconnect the power cord from the wall socket.

2. Use a flat-head screw driver or other tool to remove the Emergency Lid Release Plug, located on the right-hand side of

the unit.

3. Pull the wire (attached to the plug) to open the lid lock manually.

8.4. Setting the Time

Operating time can be selected from 0.5 min. to 99 min. or continuous. Press the Time button to change the time setting. The time

on the display will flash. Turn the Start/Stop knob to increase or decrease the value. Press the Time button again to confirm the

selection or press Start/Stop knob to store and run the selection. The flashing of time value will stop and the time value will be stored

automatically if no button is pressed after 6 seconds of the adjustment.

Operating time can be set as continuous by turning the Start/Stop knob clockwise or counterclockwise to make the time to display

“- -“. When the time is set as continuous, the centrifuge will continue to run without a stop. Press Start/Stop knob to end the

continuous operation.

8.5. Setting the Speed (RPM)

The speed (RPM) can be selected from 500 to 13,500 rpm. Press the RPM button to change the speed setting. The speed (RPM) on the

display will flash. Turn the Start/Stop knob to increase or decrease the value. Press the RPM button again to confirm the selection or

press the Start/Stop knob to store and run the selection. The flashing of the RPM value will stop, and the RPM value will be stored

automatically if no button is pressed after 6 seconds of the adjustment.

8.6. Setting the Centrifugal Force (RCF)

The speed can be selected from 20 to 17,135 x g. Press the RCF button to change the RCF setting. The RCF on the display will flash.

Turn the Start/Stop knob to increase or decrease the value. Press the RCF button again to confirm the selection or press the Start/Stop

knob to store and run the selection. The flashing of the RCF value will stop, and the RCF value will be stored automatically if no button

is pressed after 6 seconds of the adjustment.

8.7. Setting the Temperature (Temp)

The temperature can be selected from -10°C to 40°C. Press the Temp button to change the temperature setting. The temperature

on the display will flash. Turn the Start/Stop knob to increase or decrease the value. Press the Temp button again to confirm the

selection or press Start/Stop knob to store and run the selection. The flashing of the Temp value will stop, and the value will be stored

automatically if no button is pressed after 6 seconds of the adjustment.

6| LABNET PRISM R REFRIGERATED MICROCENTRIFUGE

8.8. Cooling

The temperature can be set from -10°C to 40°C. The set temperature can also be changed during the run.

Pre-cooling

Pre-cooling starts a temperature-control run at 6,000 rpm. It allows the rotor to be cooled down to the new, pre-set temperature

quickly. Cooling down from room temperature to 4°C takes approximately 8 min. (max. 16 minutes). To activate the Pre-cooling

function, press and hold the Temp button, then press the Start/Stop knob. Both RPM and Temp icons will flash during Pre-cooling. The

Pre-cooling can be stopped by pressing the Start/Stop knob. The Pre-cooling will function only when the set temperature is lower than

the ambient temperature.

Standby Cooling

When the centrifuge is powered on, the refrigeration system will cool down the chamber to the pre-set temperature before or after

the run if the chamber temperature is above the pre-set temperature.

NOTES:

To extend the life cycle of the compressor, always remember to inactivate the Standby Cooling (by increasing the set temperature

above room temperature) or power off the centrifuge when it is not in use.

When the lid is open, the Standby Cooling will stop after 3 minutes.

8.9 Starting and Stopping a Run

To start a run, press the Start/Stop knob.

When the pre-set time expires, the centrifuge will stop automatically.

To stop the centrifuge prior to the expiration of set time, press the Start/Stop knob again.

When the time is set as continuous ( - - ), the centrifuge will run continuously. To stop the continuous run, press the Start/Stop knob.

By pressing and holding the Quick button, the centrifuge will continue a short-run of set speed. The short-run will stop if the Quick

button is released.

NOTE: For safety reasons, the Start/Stop knob will be inactivated when the lid is open. A symbol “Lid“ will be flashing until the lid is

closed.

8.10. Quick-Spin Operation

The centrifuge can be operated for a short run by pressing and holding the Quick button. The centrifuge will continue to run as long

as the Quick button is depressed and the time, in seconds, will count up on the time display. The Quick-Spin speed and temperature is

based on the last run settings remaining in the memory. The icon RPM will flash during the Quick-Spin operation.

8.11. Changing the Settings During a Run

Users can change the settings while the rotor is running. Through a one-time pressing of any function buttons (Temp, Time, RPM, RCF),

the current value will switch into the pre-set value mode.

The current value flashes, and the new values can be input. Once the display switches into the current value mode after completion of

the entered values, the new values are activated. The new values are stored and executed by pressing any function button again or by

waiting 6 seconds.

Instruction Manual | 7

9.0 Service and Maintenance

9.1 Centrifuge Service

The brushless motor in this microcentrifuge requires no routine maintenance. Any required service should be performed by

authorized, qualified personnel only. Repairs performed by unauthorized personnel may void the warranty.

9.2 Cleaning

Always keep the centrifuge housing, rotor chamber, rotor and rotor accessories clean. All parts should be wiped down periodically

with a soft cloth. For more thorough cleaning, use a neutral cleaning agent (pH between 6 and 8) applied with a soft cloth. Excessive

amounts of liquid should be avoided. Liquid should not come into contact with the motor. After cleaning, ensure that all parts are

dried thoroughly by hand or in a warm air cabinet (maximum temperature 50°C).

The rotor should be cleaned after each use. When spinning samples containing phenol or phenol chloroform, the rotor should be

cleaned immediately after use.

Should a spill of infectious materials occur within the rotor or chamber, the unit should be disinfected. This should be performed by

qualified personnel with proper protective equipment.

9.3 Replacing Fuses

Check the fuse when it is recommended (see Troubleshooting Guide section). Disconnect the power cord from the wall socket before

checking the fuses. The fuse holder is located in the power inlet on the rear of the unit. Open the fuse holder drawer by inserting a

small screwdriver under the tab and prying it open. Remove the innermost (operative) fuse from its retaining tabs, and replace the

fuse if necessary. A spare fuse is located in the outermost chamber of the fuse drawer. Replace only with a fuse of exactly the same

value as the original. See Section 3.0 for information on fuse type.

10.0 Troubleshooting Guide

Problem Possible Cause Solution

Centrifuge will not start No power supply Check if power is being supplied to the outlet.

Check if the power cord is plugged into both the wall

outlet and the back of the centrifuge.

Check if the power cord is damaged.

Blown fuse Check the fuse and replace if necessary.

Lid lock will not release Defective lid lock Open manually, and have the unit serviced.

No power from the PC board Call for service.

Lid lock is jammed Call for service.

Centrifuge is not receiving power See “Centrifuge will not start”.

Centrifuge cannot be started,

although power is On

Lid is not closed correctly Close the lid correctly.

No speed or time has been selected Set the speed and/or time.

bAL (Error Message) indicates

imbalance

Tubes are not inserted symmetrically

in the rotor holes

Load the tubes symmetrically (see Section 6.3: Loading the

Rotor).

Sample liquid in the tubes is not

balanced

Make sure the same volume of liquid is in each tube.

Defective or improperly adjusted

balance sensor

Call for service.

Rotor is stuck when a run starts. Call for service.

Lid (Error Message) lid not

closed

Lid is not closed completely Close the lid.

Lid lock or sensor is defective Call for service.

Other Error Messages Er on

Display

–Press the time or speed button to clear the error.

Call for service.

Should you have a question about the operation of the Labnet Prism™ R refrigerated microcentrifuge or if service is required, contact

Corning Customer Service. Do not send in a unit for service without first calling to obtain a repair authorization number. Should the

unit require return for service, it should be properly packed to avoid damage. Any damage resulting from improper packaging shall be

the responsibility of the user.

8| LABNET PRISM R REFRIGERATED MICROCENTRIFUGE

11.0 Determination of G-values

The centrifuging radius of the 1.5/2.0 mL rotor is 8.23 cm. The Labnet Prism™ R refrigerated microcentrifuge has an automatic g-force

conversion program, so g-values are automatically calculated and can be displayed on the control panel, for this centrifuging radius.

If adapters or smaller tubes are used, the centrifuging radius changes as does the g-force. The chart below can be used to manually

determine g-values for any centrifuging radius.

500

475

450

425

400

375

350

325

25,000

20,000

15,000

10,000

6000

4000

3000

2000

1000

500

200

300

275

250

225

200

175

150

140

130

120

110

100

20,000

30,000

10,000

5000

2000

1000

500

200

100

Speed of Centrifuge

Spindle in RPM

Relative Centrifugal

Force (x g)

Radius in mm from Center of

Centrifuge Spindle to Point

Along Tube or Bottle

To calculate the RCF value at any point along the tube or bottle, measure the radius (in mm), from the center of the centrifuge spindle

to the particular point. Draw a line from the radius value on the right-hand column to the appropriate centrifuge speed on the left-

hand column. The RCF value is the point where the line crosses the center column. The nomogram is based on the formula:

RCF = (11/17 x 10-7) RN2

where:

R = Radius in mm from centrifuge spindle to point in tube bottom

N = Speed of spindle in RPM

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