Leica DM5500 B Bedienungsanleitung

Leica DM5500 B

Leica DM6000 B

Leica DM6000 M

Operating Manual

Bedienungsanleitung

Manuel d’utilisation

Published September 2007 by:

Herausgegeben September 2007 von:

Publié en septembre 2007 par :

Leica Microsystems CMS GmbH

Ernst-Leitz-Straße

D-35578 Wetzlar (Germany)

Responsible for contents:

Verantwortlich für den Inhalt:

Responsable du contenu :

Dr. Jasna Roeth, Stefan Motyka

(Product manager „Life Science Research“)

(Produktmanager „Life Science Research“)

(Chef de produit „Life Science Research“)

Holger Grasse

(Safety Officer according to MPG §30)

(Sicherheitsbeauftragter nach MPG §30)

(Officier de sécurité selon MPG §30)

In case of questions, please contact the hotline:

Bei Fragen wenden Sie sich bitte an die Hotline:

Pour toute question, contacter l’assistance en ligne: Phone +49(0)64 41-29 22 86

Fax +49(0)64 41-29 22 55

E-Mail: [email protected]

Leica DM5500 B

Leica DM6000 B

Leica DM6000 M

Operating Manual

Copyrights

All rights to this documentation are held by

Leica Microsystems CMS GmbH. Reproduction

of text or illustrations (in whole or in part) by

print, photocopy, microfilm or other method (in-

cluding electronic systems) is not allowed with-

out express written permission from Leica

Microsystems CMS GmbH.

The term "Windows" can be used in the follow-

ing text without further identification. It is a reg-

istered trademark of the Microsoft Corporation.

Otherwise, no inference with regard to the free

usability of product names may be drawn from

the use of those names.

The instructions contained in the following

documentation reflect state-of-the-art technol-

ogy and knowledge standards. We have com-

piled the texts and illustrations as accurately as

possible. Nevertheless, no liability of any kind

may be assumed for the accuracy of this manu-

al’s contents. Still, we are always grateful for

comments and suggestions regarding potential

mistakes within this documentation.

The information in this manual is subject to modifi-

cation at any time and without notification.

Contents

7. Startup ......................................................... 39

7.1 Functional Principle .................................. 39

7.2 Switching on............................................... 46

7.3 The Leica SmartTouch .............................. 47

7.4 The Function Keys at the stand .............. 48

7.5 The Remote Control Element

SmartMove ................................................. 49

7.6 Köhler Illumination .................................... 49

7.6.1 Transmitted Light ............................ 49

7.6.2 Incident Light................................... 51

7.7 Checking Phase Contrast Rings ............. 52

7.8 Adjusting Motorized Polarizer ................ 54

7.9 Adjusting the Light Sources .................... 54

8. Operation .................................................... 60

8.1 Switching on............................................... 60

8.2 Stages and Specimen Displacement .... 62

8.2.1 Manual stage (DM5500 B) ............ 62

8.2.2 Motorized stage (DM6000 B/M)... 63

8.3 Focusing ...................................................... 64

8.4 Tubes ........................................................... 65

8.5 Eyepieces.................................................... 67

8.6 Objectives ................................................... 67

8.7 Magnification Changer............................. 70

8.8 Light Sources ............................................. 70

8.9 Aperture Diaphragm and

Field Diaphragm ......................................... 71

Contents

1. Important Notes about this Manual ...... 7

2. Intended Purpose of Microscopes ........ 8

3. Safety Notes ............................................... 9

3.1 General Safety Notes ............................... 9

3.2 Electrical Safety ........................................ 10

3.3 Disposal....................................................... 11

4. Overview of the Instrument .................... 12

5. Unpacking the Microscope .................... 18

6. Assembling the Microscope .................. 20

6.1 Stage ............................................................ 21

6.2 Condenser ................................................... 22

6.3 Tube and Eyepieces.................................. 23

6.4 Objectives ................................................... 24

6.5 Light Sources for the

Transmitted Light Axis.............................. 24

6.6 Light Sources for the

Incident Light Axis..................................... 26

6.6.1 106 z lamp housing ......................... 26

6.6.2 External light source EL6000 ........ 31

6.7 Equipping the

Incident Light Turret Disc ........................ 32

6.8 Polarizer and Analyzer ............................. 33

6.9 DIC Prisms .................................................. 34

6.10 Optional Accessories ............................... 35

6.11 Connecting the Leica CTR5500/CTR6000

Electronics Box.......................................... 37

6.12 Connecting the Computer ........................ 38

6.13 Connection to the Power Supply............ 38

Contents

9. Contrasting Methods for

Leica DM5500 B and DM6000 B ............. 72

9.1 Transmitted Light....................................... 72

9.1.1 Bright Field ....................................... 72

9.1.2 Phase Contrast................................ 73

9.1.3 Dark Field ......................................... 73

9.1.4 Polarization ...................................... 74

9.1.5 Differential Interference Contrast .. 75

9.2 Fluorescence.............................................. 76

9.3 Combi Mode ............................................... 77

10. Contrasting Methods for

Leica DM6000 M ........................................ 78

10.1 Incident Light ............................................. 78

10.1.1Bright Field ....................................... 78

10.1.2Dark Field ......................................... 78

10.1.3Polarization ...................................... 79

10.1.4Interference Contrast .................... 80

10.1.5Fluorescence ................................... 80

10.2 Transmitted Light....................................... 81

10.2.1Bright Field ....................................... 81

10.2.2Polarization ...................................... 81

11. Trouble Shooting ....................................... 82

12. Care of the Microscope ........................... 85

12.1 Dust Cover .................................................. 85

12.2 Cleaning....................................................... 85

12.3 Handling Acids and Bases ...................... 86

13. Essential Wear and Spare Parts............ 87

14. Abbreviations and Pictograms .............. 88

15. Index ............................................................ 90

16. EU Declaration of Conformity................. 91

1. Important Notes about this Manual

(1.2)

→

p.20

Numbers in parentheses, such as "(1.2)", corre-

spond to illustrations (in the example, Figure 1,

Item 2).

Numbers with pointer arrows (for example

→p.20), point to a certain page of this manual.

Caution!

Special safety instructions within this

manual are indicated with the triangle sym-

bol shown here, and have a gray back-

ground.

Caution! The microscope and accessories can

be damaged when operated incorrectly.

Notes on how to dispose of the microscope,

its components and expendables.

Explanatory note.

Item not contained in all configurations

(optional).

Text symbols, pictograms and their meanings:

Caution!

This operating manual is an essential com-

ponent of the microscope, and must be read

carefully before the microscope is assem-

bled, put into operation or used.

1. Important Notes about this Manual

This operating manual contains important in-

structions and information for the operational

safety and maintenance of the microscope and

accessories. Therefore, it must be kept and

taken care of.

For the operation of the LeicaScreen and the

Leica Application Suite (LAS), please see

separate operating manual.

2. Intended Purpose of Microscopes

The Leica DM5500 B and DM6000 B micro-

scopes, to which this user manual belongs, are

designed for biological routine and research

applications. This includes the examination of

samples taken from the human body with a view

to providing information on physiological or

pathological states or congenital abnormalities,

or to determining the safety and compatibility

with potential recipients, or to monitoring thera-

peutic measures.

The Leica DM6000 M microscope is designed for

examinations in the field of materials research.

All the above-named microscopes comply with

the Council Directive 98/79/EEC concerning in

vitro diagnostics. They also conform to the

Council Directives 73/23/EEC concerning electri-

cal apparatus and 89/336/EEC concerning elec-

tromagnetic compatibility for use in an industrial

environment.

Caution!

The manufacturer assumes no liability for

damage caused by, or any risks arising from

using the microscopes for other purposes

than those for which they are intended or

not using them within the specifications of

Leica Microsystems CMS GmbH.

In such cases the conformity declaration

shall cease to be valid.

Caution!

These (IVD) devices are not intended for use

in the patient environment defined by DIN

VDE 0100-710. Neither are they intended for

combining with medical devices according

to EN 60601-1. If a microscope is electrically

connected to a medical device according to

EN 60601-1, the requirements defined in

EN 60601-1-1 shall apply.

3. Safety Notes

3.1 General Safety Notes

This safety class 1 device is constructed and

tested in accordance with

EN 61010-2-101:2002,

EN 61010-1:2001,

IEC 1010-1:2001,

safety regulations for electrical measuring, con-

trol, and laboratory devices.

Caution!

In order to maintain this condition and to en-

sure safe operation, the user must follow the

instructions and warnings contained in this

operating manual.

Caution!

The devices and accessories described in

this operating manual have been tested for

safety and potential hazards.

The responsible Leica affiliate or the main

plant in Wetzlar must be consulted when-

ever the device is altered, modified or used

in conjunction with non-Leica components

that are outside of the scope of this manual.

Unauthorized alterations to the device or

noncompliant use shall void all rights to any

warranty claims!

3. Safety Notes

Leica EL6000

For indoor use only.

Supply voltage:

Frequency:

Power consumption:

Fuses:

Ambient temperature:

Relative humidity:

Overvoltage category:

Contamination class:

(see enclosed manual)

Caution!

The power plug may only be plugged into an

outlet equipped with a grounding contact.

Do not interfere with the grounding function

by using an extension cord without a ground

wire. Any interruption of the ground wire in-

side or outside of the device, or release of

the ground wire connection, can cause the

device to become hazardous. Intentional

ground interruption is not permitted!

3.2 Electrical Safety

General specifications

Leica CTR5500 and CTR6000 electronics box

For indoor use only.

Supply voltage:

Frequency:

Power input:

Fuses:

Ambient temperature:

Relative humidity:

Overvoltage category:

Pollution degree:

Microscope

For indoor use only.

Supply voltage:

Frequency:

Power input:

Fuses:

Ambient temperature:

Relative humidity:

Overvoltage category:

Pollution degree:

Supply unit ebq 100*

For indoor use only.

Supply voltage:

Frequency:

Power input:

Fuses:

Ambient temperature:

Relative humidity:

Overvoltage category:

Pollution degree:

(see enclosed manual)

90-250 V~

50-60 Hz

max. 290 VA

T6,3 A

(IEC 60127-2/3)

15-35°C

max. 80% to 30°C

90-250 V~

50-60 Hz

See CTR5500/6000

15-35°C

max. 80% to 30°C

90-250 V~

50-60 Hz

max. 155 VA

2xT2A (IEC 127)

10-36°C

max. 80% to 30°C

100-240 V~ (±10%)

50-60 Hz

max. 210 VA

5x20, 2.5 A, slow,

breaking capacity H

→EL6000 manual

0°-40°C

10-90%

non-condensing

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