Nikon A1R Bedienungsanleitung

The Nikon A1R User Guide

General Guidelines:

If you get stuck at any point, do not understand something or find the system does not

function properly ALWAYS warn the staff!!!

Objectives:

1. Objectives are very expensive always handle with extreme delicacy especially while changing

samples. Never touch or scratch the front lens directly.

2. When starting to work check for visible damage.

3. Always start imaging with the 4x objective and proceed to larger magnifications. This helps to

avoid crashing into the sample and damaging the objective.

4. When changing objectives always make sure the distance between the objective and stage is

appropriate. ESPECIALLY if using a different stage insert than the previous user.

5. When applying oil always check if the objective is indeed an oil immersion objective.

6. After using the 60x oil immersion lens NEVER forget to clean with lens cleaning paper: 1. Fold

the lens cleaning paper three times to form a strip, 2. Hold it on the two ends on both sides, 3.

gently wipe with left-right motion. Never press with your finger directly. 4. Repeat previous

step until you cannot see oil stain and repeat once after that one final time.

7. When finished ALWAYS turn to the 4x objective and lower the stage to a safe distance.

Sample Holder:

1. When using the universal sample holder (Ti-SH-U) never loosen the left slider!! This is the

fixed point and changing it'sposition will prohibit users to find positions when reimaging slides.

Lasers:

1. Only switch on lasers that will be used for your experiments. Currently only the 488nm (green)

and 647nm (far red) lasers can be controlled independently.

Epifluorescent Illumination:

1. Only switch on the epifluorescent lamp if you will use it and turn it off if it is not needed by

you or the next user.

2. The lamp needs 15 minutes of cooldown between on and off cycles. Please take care as this

will prolong the life of the arch lamp.

Computers:

1. Never save data on the local hard drives. Please use the network drives instead.

Booking:

1. When switching off always check the calendar. Users might delete their booking during your

time slot. If that happens to be in the afternoon the system might stay. This is potentially

dangerous and waists the lifetime of the lasers. If you delete your booking please make sure to

warn the previous user!!

2. When deleting a booking please send an e-mail to the previous/subsequent user and the institute

mailing list.

3. IN-VIVO experiments can be only started in the AFTERNOON as the drift caused by cooling

and heat within the chamber will affect following users as well.

System Start up:

Step 1.: Switch on the control computer.

Step 2.: Power up the laser board by turning the key on the controller front panel of the

laser unit. This will bring the 405nm (Blue) and 561nm (Red) lasers ON. The 488nm

(green) and 647nm (far red) lasers will be in standby mode.

Step 3.: Power up the lasers that will be used for the current experiment. To prolong the

lifetime of the laser heads make sure to ONLY switch on the lasers that are really needed!

Currently only the 488nm (green) and 647nm (far red) lasers can be controlled

independently. The 647nm (far red) laser can be powered up with a key on the remote on

the laser board. To switch on the 488nm Argon Ion laser switch the "discharge" flip switch

of the controller on the laser board unit to ON with the. The laser will start in about 20

seconds (the output power is about 17-18 mW that will appear on the screen of the

controller.

Step 4.: Switch on the confocal controller that is situated to the left of the microscope. The

switch is on the left side roughly in the middle.

Step 5.: Switch on the stage controller on the table and wait 10 seconds. Waiting is

important as Nikon microscopes are highly customizable and the microscope body checks

which peripheries are currently connected.

Step 6.: Switch on the Microscope body with the RED switch on the optical table.

Step 7.(Optional): If you will use diascopic illumination switch the Halogen lamp on. The

controller is on the table by the microscope.

Step 8.(Optional): If you will use the Epifluorescent illumination switch on the lamp (light

unit is on the table by the microscope). Beware that the lamp needs 15 minutes of cooldown

after being switched of (so check with the previous user). Please also switch off the lamp if

you do not need it within a reasonable amount of time.

Step 9.: Log on to the network and start NIS elements. Please NEVER save data to the

local hard drives as they might be deleted!!

System Shut Down:

Step 1.: ONLY proceed if you are the last user or if the instrument is not booked for an

hour or more.

Step 2.: ALWAYS switch back to the 4X objective and distance the objective from the

stage. If the 60x oil immersion lens has been used NEVER forget to clean with lens cleaning

paper: 1. Fold the lens cleaning paper three times to form a strip, 2. Hold it on the two ends

on both sides, 3. Gently wipe with left-right motion. Never press with your finger directly.

4. Repeat previous step until you cannot see oil stain and repeat once after that one final

time.

Step 3.: Stop NIS elements. If you proceed with the shutdown process without exiting NIS

Elements it will prompt you with error messages.

Step 4.: Switch the epifluorescent lamp OFF.

Step 5.: Switch the Halogen lamp OFF.

Step 6.: Switch OFF the Microscope body with the switch on the optical table.

Step 7.: Switch OFF the stage controller and wait 10 seconds.

Step 8.: Switch OFF the confocal controller besides the lasers by pressing the button on the

front panel.

Step 9.:

Power OFF the 488nm (green) and 647nm (far red) lasers. The 647nm (far red) laser can

be powered OFF with the key on the remote control. The 488nm Argon Ion laser switched

to standby mode by switching the "discharge" flip switch to OFF on the controller on the

laser board unit.,

Step 10.: Shut down the laser board with the key on its front panel.

Step 11.: Switch off the computer and check if everything is off before leaving.

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