Olympus BX-RFAA Bedienungsanleitung

INSTRUCTIONS

A X 9 9 4 8

This instruction manual is for the Olympus Motori ed Reflected Fluorescence System. To ensure the

safety, obtain optimum performance and to familiari e yourself fully with the use of this system, we

recommend that you study this manual thoroughly before operating the system. Retain this instruction

manual in an easily accessible place near the work desk for future reference.

BX-RFAA

U-LH100HGAPO

U-LH100G

Power Supply Unit

U-25ND

U-25ND25

U-RSL

U-RSL EM

BX-RFSS

U-EXBABG

U-EXBAUB

U-EXBAUG

MOTORIZED REFLECTED

FLUORESCENCE

SYSTEM

CONTENTS

IMPORTANT — Be sure to read this section for safe use of the equipment. — 1-3

Correct assembly and adjustments are critical for a reflected fluorescence system to exhibit its full performance. If you are

going to assemble the microscope yourself, please read chapter 9, “ASSE BLY” (pages 29 to 35) carefully.

1 NOMENC ATURE

3 USING THE CONTRO S

4 SIMU TANEOUS F UORESCENCE OBSERVATION METHODS

5 TROUB ESHOOTING GUIDE

6 SPECTRA CHARACTERISTICS OF FI TERS

7 SPECIFICATIONS

4-5

6-7

8-14

16-17

18-21

1General Precautions for Observation....................................................................................................................... 8

2Selecting of the Fluorescence irror Unit.................................................................................................. 8-9

3Objectives for Various Observation odes ................................................................................................. 10

4Turning the Power Supply Unit On............................................................................................................................ 10

5Centering the Field Iris Diaphragm ........................................................................................................................... 11

6Centering the Aperture Iris Diaphragm .............................................................................................................. 12

7Centering the ercury Burner ................................................................................................................................ 13-14

8Using the ND Filters......................................................................................................................................................................... 14

1Simultaneous Reflected Fluorescence and Phase Contrast Observations 15

Simultaneous Reflected Fluorescence and Transmitted Nomarski DIC Observations

.... 15

REF ECTED F UORESCENCE OBSERVATION PROCEDURE

9 ASSEMB Y

— See this section for the replacement of the light bulb. —

9-1 Assembly Diagram ............................................................................................................................................................................ 29

9-2 Detailed Assembly Procedures ............................................................................................................................ 30-35

29-35

■ PROPER SE ECTION OF THE POWER SUPP Y CORD .................................................................... 36-37

8 OPTIONA MODU ES 23-28

16-Position Filter Slider U-RSL6 ............................................................................................................................. 23-24

26-Position Barrier Filter Slider U-RSL6E ...................................................................................................... 25

Rectangular Field Stop Unit BX-RFSS (for exclusive use with the BX-RFA/RFAA)

....... 26

Exciter Balancers U-EXBABG/EXBAUB/EXBAUG (for exclusive use with the BX-RFA/RFAA)

............ 27-28

IMPORTANT

The motorized operations of the BX-RFAA motorized reflected fluorescence illuminator used with this

system can be controlled from the BX-UCB control bo and U-HSTR2 hand switch (the illuminator can

also be interlocked with other motorized equipment using the BX2 software for PC).

Motorized operations: · Turret switching of mirror units

· Insertion/removal of the shutter

· Activation of motorized revolving nosepiece connector

SAFETY PRECAUTIONS

1. A microscope with motorized parts is a precision instrument. Handle it with extreme care and avoid subjecting it to

impact.

2. For the ultrahigh-pressure mercury burner use the Olympus-provided USH102D (manufactured by USHIO) or HBO103W/

2 (manufactured by OSRAM) DC burner.

3. Make sure that the mercury burner is attached and cords are connected.

4. The internal parts become very hot during and within 10 minutes after completion of operation. Do not attempt to open

the light housing in these periods (see page 10).

5. The stoppers provided for some functions may be damaged if excessive force is applied.

6. Do not disassemble the power supply unit because it contains high voltage parts inside.

7. Always use the power cord provided by Olympus. If no power cord is provided please select the proper power cord by

referring to the chapter “PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of this instruction manual. If the

proper power cord is not used product safety and performance cannot be guaranteed.

8. Always ensure that the grounding terminal of the power supply unit and that of the wall outlet are properly connected. If

they is not grounded Olympus can no longer warrant the electrical safety and performance of the equipment.

9. Before opening the lamp housing for replacing the burner etc. be sure to set the main switch to “ ” (OFF) unplug the

power supply unit’s output connector for the power supply to the lamp housing and wait for more than 10 minutes until

the burner has cooled down.

The top panel of the lamp housing becomes very hot during operation. To prevent fire hazard do not block the ventilation

through the top panel.

Symbol E planation

Safety Symbols

The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment

in the safest possible manner.

High voltage warning indicating presence of high voltage (1 kV or more).

Indicates that the surface becomes hot and should not be touched with bare hands.

Before use carefully read the instruction manual. Improper use could result in personal injury to

the user and/or damage to the equipment.

Indicates that the main switch is ON.

Indicates that the main switch is OFF.

10.

Warnings

Warning engravings are placed at parts where special precaution is required when handling and using the system.

Always heed the warnings.

Warning engraving

position

· Lamp housing

(U-LH100HG, U-LH100HGAPO)

· ND filter

(U-25ND6, U-25ND25)

· Power supply unit

(for 100 W mercury burner)

[Warning against

high temperature]

[Warning against

high voltage]

1 Getting Ready

1. This manual pertains only to the operations of the motorized reflected fluorescence system. Also read the instruction

manuals of the BX microscope and associated options to obtain general understanding on the system.

2. Do not attempt to operate the motorized parts manually. They may be destroyed by excessive force.

3. The motorized reflected fluorescence system is composed of precision instruments. Handle them with care and avoid

subjecting them to sudden or severe impact.

4. Do not use the system where it is subjected to direct sunlight high temperature and humidity dust or vibrations.

5. To enable ventilation for the lamp housing and power supply unit reserve spaces of more than 10 cm around them.

6. The power cord can be used to shut off power supply in case of emergency. To enable this function install the power

supply unit so that its power cord connector (on the rear of the power supply unit) or wall power outlet can easily

accessed and unplugged in case of emergency.

2 Maintenance and Storage

1. Clean all glass components by wiping gently with gauze. To remove fingerprints or oil smudges wipe with gauze slightly

moistened with a mixture of ether (70%) and alcohol (30%).

Since solvents such as ether and alcohol are highly flammable, they must be handled carefully. Be sure to keep

these chemicals away from open flames or potential sources of electrical sparks –– for e ample, electrical

equipment that is being switched on or off. Also remember to always use these chemicals only in a well-venti-

lated room.

2. Do not attempt to use organic solvents to clean the system components other than the glass components. To clean

them use a clean cloth. If they are extremely dirty use a soft cloth slightly moistened with a diluted neutral detergent.

3. Never attempt to disassemble any part of the system.

4. When the hour counter on the power supply unit indicates 200 hours (USH102D) or 300 hours (HBO103W/2) set the main

switch to “ ” (OFF) wait for more than 10 minutes for safety then replace the mercury burner (see page 33). Unlike

fluorescent lamps the mercury burner incorporates high-pressure gas sealer inside it. If the burner is used far beyond the

service life the distortion will accumulate in the glass tube which may explode in worst case.

3 Applicable Intermediate Attachment

The BX-RFAA motorized reflected fluorescence illuminator can be used in two-stage stacked configuration by attaching

an intermediate attachment on it.

4 Caution

If the system is used in a manner not specified by this manual the safety of the user may be imperiled. In addition the

equipment may also be damaged. Always use the equipment as outlined in this instruction manual.

The following symbols are used to set off text in this instruction manual.

: Indicates that failure to follow the instructions in the warning could result in bodily harm to the

user and/or damage to equipment (including objects in the vicinity of the equipment).

# : Indicates that failure to follow the instructions could result in damage to equipment.

} : Indicates commentary (for ease of operation and maintenance).

NOMENCLATURE

Analyzer/6-position barrier

filter insertion slot (Page 25)

Motorize Revolving Nosepiece

Mercury Lamp Housing

U-LH100HGAPO

U-LH100HG

Motorize Reflecte

Fluorescence Illuminator

BX-RFAA

Mirror unit indication

window

UV cut plate

Mirror unit indication

pockets (Page 31)

( 6)

Field iris diaphragm knob (Page 11)

Aperture iris diaphragm knob (Page 12)

Collector lens focusing knob (Page 13)

Burner centering

knobs (Page 13)

Mirror focusing screw

(Page 13)

(On the rear of lamp

housing)

ND filter/e citer balancer insertion slot

6-position filter insertion slot (Page 23)

Aperture iris diaphragm centering screws (Page 12)

Field iris diaphragm centering screws (Page 11)

( 2)

(Page 27)

Indication stickers

}The BX-RFAA can accept up to six fluorescence mirror units.

# Every mirror unit is composed of a dichroic mirror, barrier filter and

e citation filter which are suitable for each e citation method. Basi-

cally, do not disassemble the mirror unit.

}If you want to fabricate an original fluorescence mirror unit it is recom-

mended to use the U-MF2 empty mirror unit (which does not include

filter) (page 32).

A blank indication stickers are provided with the illuminator. Use the stickers

for writing the original mirror unit names.

Lamp ON LED

Input

connector

ND Filter

U-25ND6

U-25ND25

Power Supply Unit

(for 100 W mercury burner)

Fluorescence Mirror Unit

18 mo els inclu ing the U-MWU2

Centering Target

U-CST

Hour counter

Main switch

“I”: ON

“ ”: OFF

Output

connector

REFLECTED FLUORESCENCE OBSERVATION

PROCEDURE

}For the operating procedures for simultaneous observations of phase contrast or transmitted Nomarski DIC observation

with fluorescence observation see chapter 4 “SIMULTANEOUS FLUORESCENCE OBSERVATION METHODS” (page 15).

(Controls Used) (Page)

Preparation

· Attach the fluorescence mirror units and objectives which are suitable for the observation method to be used. (P. 8 - 10)

· Center the mercury burner. (P. 13 - P. 14)

· Set the main switch @ of the BX-UCB control box.

Set the main switch to “ I ” (ON) and wait until

the arc image stabilizes (5 to 10 minutes). ² Main switch (P. 10)

Place the specimen on the stage. ³ Specimen holder

| X/Y-axis knobs

Engage the mirror unit matching the speci-

men.

ƒ MU+ (Mirror Unit Right) or

MU- (Mirror Unit Left) button

Engage the objective in the light path and bring

the specimen in focus.

… Objective button

† RSHT (shutter) button

‡ Stage UP/DOWN button

Engage a ND filter in the light path

as required. Š ND filter (P. 14/23)

Adjust the collector lens so that the field of

view is uniform and brightest. ‰ Collector lens focusing knob (P. 13)

Adjust the field iris diaphragm. ‹ Field iris diaphragm knob (P. 11)

Adjust the aperture iris diaphragm. Œ Aperture iris diaphragm knob (P. 12)

Start observation.

}Engage the shutter if you interrupt observation for a short time. † RSHT (shutter) button

} Make a photocopy of the observation procedure pages and post it near your microscope.

Mercury Lamp Housing

U-LH100HGAPO

U-LH100HG

Motorized Reflected

Fluorescence Illuminator

BX-RFAA

Motorized Revolving

Nosepiece

Microscope

BX61

BX62, etc.

Control Bo

BX-UCB

Hand Switch

U-HSTR2

Power Supply Unit

(for 100 W mercury

burner)

…

†

‡

Š‰

‹

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