Olympus FLUOVIEW FVMPE-RS Bedienungsanleitung

AX8343

Service Manual

FLUOVIEW

FVMPE-RS

Multi Photon Laser Scanning Microscope

Notice

Thank you for your purchase of Olympus microscope at this time.

Retain this manual in an easily accessible place near a system for future reference.

- Research use only -

FVMPE-RS

Important

Caution

This system is a CLASS 4 laser product.

The procedures for using this system are classified as follows:

• Operation

"Operation" means the operations of observation functions using the microscope described in the FVMPE-RS user's

manual provided by Olympus.

CLASS 4 laser light is only emitted from the objective lens during the actual execution.

• Maintenance

“"Maintenance" means the adjustment or other procedures performed by customers to maintain the proper opera-

tions of this system described in the FVMPE-RS User's Manual provided by Olympus.

• Service

"Service" means any adjustment or repair performed by Olympus service personnel or technical personnel who are

provided the service training according to the service manual provided by Olympus.

The performance has influence on the feature of this system, and there is a risk that unintended CLASS 4 laser light is

emitted.

In order to maintain the full performance of this system and ensure your safety, be sure to read this service manual, the

FVMPE-RS user's manual, and the operating instructions for the laser unit and light source unit before use.

For this unit, other than the manuals and instructions above, there is also an on-screen manual ("Online Help") embed-

ded in the software. For details of FV30S-SW and the touch panel controller, refer to Online Help.

Reproduction, copying or duplication of a part or all of this software and manual is prohibited.

This is a Service Manual for Multi Photon Laser Scanning Microscope FVMPE-RS in FLUOVIEW Series.

This manual is intended to be used by Olympus service personnel or technical personnel who are provided the service

training from Olympus.

Registered trademarks

Microsoft, Microsoft Windows, Excel for Windows are registered trademarks of Microsoft Corporation.

Mai Tai®, InSightTM, and DeepSeeTM are registered trademarks of Newport Corporation.

ChameleonTM, OBISTM, and SapphireTM are registered trademarks of Cohelent Inc..

ProScanTM is registered trademark of Prior Scientific Ltd..

Other brand names and product names are trademarks or registered trademarks of their respective owners.

Caution

If the system is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the

system may also be damaged. Always use the system as outlined in this instruction manual.

The following symbols are used to set off text in this instruction manual.

: Indicates a potentially hazardous situation which, if not avoided, may result in minor or

serious injuries such as blindness, etc., or damage to the equipment or other property.

It may also be used to alert against unsafe practices.

} : Indicates commentary (for ease of operation and maintenance).

CAUTION

FVMPE-RS

1 Before starting service work ...............................................................................................................................1

1-1 Laser classes and danger levels ..................................................................................................................................................1

2 Service work .........................................................................................................................................................................3

2-1 Work related to the microscope ....................................................................................................................................................3

1 Work related to the nosepiece ...................................................................................................................................................3

2-2 Work related to the illumination......................................................................................................................................................7

1 Changing the halogen bulb ..........................................................................................................................................................7

2 Changing the mercury lamp .........................................................................................................................................................9

2-3 Work related to optical unit ..................................................................................................................................................................11

1 Fluorescence mirror unit ..................................................................................................................................................................12

2 DM unit for the SIM scanner .........................................................................................................................................................14

3 DM unit for external NDD .................................................................................................................................................................16

2-4 Work related to laser introduction ................................................................................................................................................18

1 Opening and closing the laser port (FV30-LPADP) ...................................................................................................18

Contents

FVMPE-RS

1 Before starting service work

This system is a Class 4 laser product. These products may expose the human body to dangerous laser beams. Please

be thoroughly informed about laser safety and observe the following precautions when working with these products.

During the service work, be sure to avoid those other than service workers entering around the device.

Before conducting the service work, be sure to perform the works described in "1-5 Confirmation when working with

FVMPE-RS" and "3-3 Completing the observation with this system (Operation)" in [Operation/Maintenance] in FVMPE-RS

User's Manual.

1-1 Laser classes and danger levels

• This system is a Class 4 laser device. Not only direct and specular reflection laser beams but also

diffuse reflection beams are dangerous.

• Only Olympus technical personnel are allowed to assemble and set up this system.

When people who are not Olympus technical personnel did a change (disassembly and modification)

of a device which is not mentioned in this service manual, problems such as system malfunction,

breakage or an accident may occur. We will provide disassembly, relocation and other services for a

fee.

• Keep the objectives away from anything that has a mirror surface.

• Keep the device away from any combustible gas or liquid to prevent fire.

• It is dangerous to remove the objectives, the nosepiece caps and/or nosepiece, as this causes

irradiation of laser beams that are parallel, not diffused.

•

This system uses an IR pulse laser, which has an element of danger to cause blindness if it enters the

eye.

• This system generates laser beams with a near-infrared wavelength, which are invisible and danger-

ous.

• Total laser radiation emitted from this system are as follows:

MPE (Maximum Permissible Exposure) : 0.016 J/m2 (for a single pulse)

0.00005 J/m2 (for a single pulse in the pulse train)

NOHD (Nominal Ocular Hazard Distance) : 50 cm in the vicinity of objective lens *

Exposure duration : 3 X 104 sec

* Hereafter, this area is called “Laser Hazard Area”.

CAUTION

Class Description of danger level

Class 1 Lasers are safe when any kind of optical system is used.

Class 1M Degree of danger is equal to that in Class 1 for naked-eye observation. However, observation through an

optical device is dangerous.

Class 2 This class applies to visible-light lasers with wavelengths of 400 to 700 nm, and safety is ensured by the

aversive reaction of eyes.

Class 2M Degree of danger is equal to that in Class 2, but use of an optical device is dangerous.

Class 3R Direct beam observation is potentially dangerous.

Class 3B Direct beam observation is dangerous.

Class 4

The lights of lasers of this class, being able to cause diffused reflection that is deemed dangerous, pose the

danger of causing fire as well as of damaging skin.

All laser products that can cause an amount of laser light radiation exceeding the accessible emission limit

for class 3B upon human bodies.

• Main duties of the laser safety officer to manage lasers are as follows.

Planning and implementing measures for preventing damages due to laser light

Setting and managing laser controlled area

Managing key and so on which starts laser equipment

Inspecting and maintaining laser equipment, and preserving record of them

Inspecting and maintaining protection tool, and observing if it is being used properly

Conducting labor hygiene education and preserving record of it

Other items necessary for preventing damages due to laser light

• Service work is work that can affect the performance of the device and such work is described in this

service manual.

Only Olympus service personnel or technical personnel who are provided the service training by

Olympus are allowed to do the service works according to the procedures described in this service

manual.

• Doing service work by any other procedures than those given in this manual will result in exposure to

dangerous laser radiation.

• During performing service work, do not allow others to enter the periphery of this system except

service workers.

CAUTION

FVMPE-RS

I Removing the nosepiece

1) Remove the connector attached to the cable of the nosepiece from the connector secured to the left side of

FV30-RFABXL with the binder.

2) With the motorized focusing knob of the controller (U-MCZ), move the stage apart from the objectives

without hiding the nosepiece mounting screw 1.

3) Fully loosen the nosepiece mounting screw 1 using the Allen screwdriver provided with the system.

4) Gently remove the nosepiece from the front side along the nosepiece mount dovetail.

1 Work related to the nosepiece

1 Removing and mounting the nosepiece

2 Service work

Confirm that the laser is not emitted, and then do the following work.

CAUTION

2-1 Work related to the microscope

} When a nosepiece of a different type from the removed nosepiece is mounted, it is necessary to change

the nosepiece information settings for the touch panel controller. For details, refer to Online Help of the

touch panel controller.

II Mounting the nosepiece

1) With the motorized focusing knob of the controller (U-MCZ), move the stage apart from the objectives

without hiding the nosepiece mounting screw 1.

2) Fully loosen the nosepiece mounting screw 1 using the Allen screwdriver provided with the system.

3) Gently insert the nosepiece from the front side along the nosepiece mount dovetail, and push the

nosepiece until it stops.

4) Tighten the nosepiece mounting screw 1.

5) Connect the connector attached to the cable of the nosepiece to the connector secured to the left side of

FV30-RFABXL with the binder.

➀

BX63LF

FV30GF

➀

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