Olympus CKX31 Betriebsanleitung

CULTURE MICROSCOPES

CKX31/CKX41

REPAIR MANUAL

SCIENTIFIC EQUIPMENT DIVISION

MARKETING DEPARTMENT

INTRODUCTION

The CKX31/CKX41 is a culture microscope newly adopting the UIS optical system and is

CKX31/CKX41

placed as the successor to conventional CK30/CK40. In electrical unit, the circuit board

3) Since the optical axis adjustment for binocular tube of CKX31 is basically the same as that

of CK30, the adjustment procedure is not written in this manual. Therefore, it is referred to

CK30/CK40 repair manual.

4) The above matter is also applied to CKX41. Therefore, this manual instructs the reference

page in CH3-BI45 repair manual when U-CBI30-2 is repaired.

In case where the other observation tube, which can be combined with CKX41 frame, is

repaired, refer to each corresponding repair manual.

is used, which corresponds to world-wide voltage by built-in voltage changeover switch.

This manual has been written on the premise that the service engineer already has experience

thoroughly the procedures of optical axis adjustment in CK30/40 and CH3-BI45 repair

manuals because the explanation of these parts are ommited as a common repair skill.

In optical adjustment section, parfocality adjustmet is mainly explained on D-6 and D-12.

The parfocality is required to adjust precisely due to severe standard compare with CK30/40.

2) Before optical adjustment, refer to jigs used in CKX31/ CKX41on D-4, D-9 and D-10.

5) Since the descriptions of disassembling and assembling procedures are simplified,

start disassembling according to the order of steps from (A) and assemble in reversed order

while taking care to follow the applied locations of grease/adhesive and remarks.

(The applied locations of grease/adhesive and adjustment part on components of assembly

are shown in exploded diagram.)

The “remark” column provides important notes and supplemental information.

6) Voltage adjustments will be needed when replacing the rheostat ass’y (DZ308500) or

circuit board (DZ308400) respectively. These adjustments can be made by following the

instructions given in this manual.

1) For overview of optical adjustment, refer to D-1 and D-2 in the section of repair procedure.

repairing the CK30/40 and observation tube, CH3-BI45. If not, it is necessary to read

A. OUTLINE OF PRODUCT

1. Outline..............................................................................................................................................A-1

2. Features ............................................................................................................................................A-1

3. Using conditions ...............................................................................................................................A-2

4. Specifications ...................................................................................................................................A-3

5. Dimensions .......................................................................................................................................A-4

6. CKX31/CKX41 system chart ...........................................................................................................A-5

B. INSPECTION STANDARD

1. Inspection items and methods............................................................................................................B-1

1. CKX31 frame ...................................................................................................................................C-1

2. CKX41 frame ...................................................................................................................................C-3

3. Binocular tube of CKX31 frame.......................................................................................................C-5

4. Binocular tube (U-CBI30-2) for CKX41..........................................................................................C-7

5. Coarse / fine focus adjustment knob..................................................................................................C-9

6. Focusing guide unit ......................................................................................................................... C-11

7. Electrical unit .................................................................................................................................C-13

8. Transmitted illuminator...................................................................................................................C-15

4. Electrical unit ............................................................................................................................... D-14

F. LUBRICANTS AND CHEMICALS

1. Listofgreases ................................................................. F‑1

E. JIGS AND TOOLS

2. List of adhesives ............................................................................................................................... F-1

D. REPAIR PROCEDURE

1. Overview of optical adjustment ....................................................................................................... D-1

2. Adjustment procedure (CKX31) ...................................................................................................... D-3

2-1 Preparation for optical adjustment (CKX31) .............................................................................. D-4

2-2 Checking parfocality for CKX31 ................................................................................................ D-5

2-3 Parfocality adjustment for CKX31 ............................................................................................. D-6

3. Adjustment procedure (CKX41) ...................................................................................................... D-8

3-1 Preparation for optical adjustment (CKX41) .............................................................................. D-9

3-2 Checking parfocality for CKX41 .............................................................................................. D-11

3-3 Parfocality adjustment for CKX41 ........................................................................................... D-12

CONTENTS

CKX31/CKX41

C. DISASSEMBLY AND ASSEMBLY PROCEDURES

1. List of jigs and tools.......................................................................................................................... E-1

4-1 CKX31/CKX41 connecting diagram........................................................................................ D-14

4-2 Voltage adjustment.................................................................................................................... D-14

1. Outline

(1) CKX31: Inverted culture microscope with fixed observation tube

CKX41: Inverted culture microscope with interchangeable observation tube

(successor to CK40)

Reflected light fluorescence attachment mountable

(2) Target market and applications

Tissue culture routine market represented by culture, growth, immunity and pharmacy.

(3) External standards acquired

(a) IEC1010-1

The product complies with UL. The CE mark was self-declared.

(b) UL3101-1

(d) EN50082-2(1995)

(4) Service life

8 years

2. Features

(1) UIS optical system is adopted and it brings drastic improvement in image flatness as well as

excellent clarity right to the edge, even in the case of wide field-of-view images.

(2) Use of a phase slider makes it possible to achieve high contrast images(active cell).

1) Common phase contrast ring slit(RS) is provided for 10X-40X to enable phase contrast

2) No centering is necessary for phase contrast ring slit (RS) and phase membrane in objective.

observation of 10X-40X simply by changing the objective.(when combined with IX2-SLP)

(Applicable when the CKX31/CKX41 is combined with the IX2-SLP.)

(3) Main switch and light intensity adjustment knob are located on the front of microscope frame.

Slider

Magnification

10X 20X

4X 40X

IX2-SLP dedicated RS

(built-in)

PHL

(built-in)

RS adjustment

IX2-SL

Common to 10X - 40X (built-in) Unnecessary

IX2-SLPH1

IX2-SLPHC IX2-SLPH2

(RS adjustment:

unnecessary)

Necessary

(4) Specimen in various types of containers can be observed by selecting effective observation

methods.

1) Observation can be made by using various observation methods.

Observation method Application

Brightfield observation

Phase contrast observation

Observation of suspended cells

Fluorescence observation

Observation of adhering cells

(Observation of the internal structure)

Observation of GFP appearance

(5) The working distance(W.D.) is 72mm with condenser and 150mm without condenser.

Therefore, various containers from petri dishes to roller bottle and squareflasks can be used

for observing specimens.

(6) Designed with the minimum necessary footprint, working space can be secured even in confined

space like a clean bench.

(successor to CK30)

CKX31/CKX41

CKX31/CKX41  A. OUTLINEOFPRODUCT

A-1

(7) Attaching a sub-stage(CK2-SS) to the plane stage, the necessary stage area can be set

(1)A low-priced tilting obserbation tube is provided to facilitate observation in the standing

and working space can be secured easily.

or sitting position.

(2) Fluorescence observation can be made by mounting a reflected light fluorescence attachment

(B-excitation, G-excitation). As option, it is applicable to U-excitation.

(3) The digital camera(DP12) can be attached to the trinocular tube, as can various types of

video camera.

3. Using conditions

(1)Ambient temperature:

Temperature: 0 - 40 degrees C(32-104 degrees F), Humidity: 30-90%

(2) Range of combination:As indicated by the CKX41/CKX31 system chart.

(3)Applicable objective

1) Phase contrast observation:

Applicable slider IX2-SLP

Applicable objective

UPLFL4XPHP

CACH10XPHP

LCACH20XPHP

LCACH40XPHP

CPL10XPH(PHC), PL10XPH(PH1)

LCACH20XPH(PHC), LCPLFL20XPH(PH1)

LCACH40XPH(PH2), LCPLFL40XPH(PH2)

UPLFL4XPH (PHL)

IX2-SL

*If a combination of slider and objective is changed, phase contrast effectiveness

can not be obtained.

*In a combination of IX2-SL, objective other than the above appicable objective is possible

to use as long as the objective corresponds to PHL/PHC/PH1/PH2.

2) Brightfield observation:

PLC4X, PLC10X, ACH20X, LCPLFL40X

*Since illumination does not cover the N.A. of LCPLFL40X objective, resolution is not

*In brightfield observation, objective other than the above ones can not be used.

If used, visibility is deteriorated because N.A. of objective is larger than the above

fully obtained in this objective.

applicable objective.

(4)Applicable container sizes

When a ULWCD is mounted (phase contrast observation):

70mm or less in overall height

When no ULWCD is mounted (brightfield observation):

148mm or less in overall height

CKX31/CKX41

CKX31

(1) Since the observation tube is fixed with the frame, it can not be changed.

CKX41

(1)Applicable observation tube

F.N.20: U-CBI30-2, U-CTR30-2, CKX-TBI

(1) Observation is possible in F.N.20.

CKX31

CKX41

(4) Observation is possible in F.N.20 to 22.

CKX31/CKX41 A. OUTLINEOFPRODUCT

F.N.22: U-TBI-3, U-TR30-2, U-BI30-2

(2)Applicable eyepiece

WHB10X/10X-H(F.N.20), WH10X/10X-H(F.N.22)

(when WHC10X is used, visibility on the perphery is not clear.)

A-2 2

(3)A intermediate attachment can not be attached.

When U-EPAis used in CKX40 a peripheral light intensity becomes slightly insufficient

in the following combinations.

*Binocular observation in F.N.22 with U-TBI-3 and 4X (or10X) objective.

*Large format: photography with 4X(or10X) objective and 2.5X photo eyepiece.

(4) When photographed in the transmitted illumination, it is recommended that a optional

4. Specifications

UIS optical system (infinity corrected compensation-free)

Optical system

Focus Vertical nosepiece movement (stage is fixed),

Coaxial coarse and fine focus with tension adjustment,

Item CKX41 CKX31

Roller guide mechanism

Stroke: 7mm down and 2mm up from focus position (1mm) which is

underthestage

Stroke per rotation: coarse adjustmet; 36.8mm

fineadjustmet;0.2mm

3Revoving nosepiece Quadruple

Stage Plane stage: 160mm(L) X250mm(W), stage clips attachable

exchangeableinsertplate (diameter:25mm)

 incorporated

petridishhoderstage

(diameter:35mm)

 incorporated

Mechanical stage: Right low drive coaxial controls

Stage movement:X=120mm,Y=78mm, withthreedish/sample holder

Substage: 70(L)X180(W)mm

Auto X/Y stage (spot scan) mountable

5Illumination

system Light source: 6V30W halogen bulb (6V30WHAL)

Average life time of bulb: 100 hours

Lamp socket: U-LS30-3-2

Filter holder : Detachable filter holder

 45mm filters can be inserted up to 11mm in thickness

Aperture diaphragm: lever control type

diameter 3mm - 44mm (minimum - maximum)

Slider insertion: With phase slider pocket

Built-inclickforsliderpositioning

6Condenser Detachable ultra-long working distance condenser (N.A. 0.3, W.D. 72mm)

7Observation tube

Binocular tube: U-CBI30-2, U-BI30-2

Fixed

inclined 45 degree,

interpupillary distance

range: 48-75mm,

diopter adjustment by

helicoid on right sleeve

Triocular tube: U-CTR30-2, U-TR30-2

Circular dovetail attachment

Tilting binocular tube: U-TBI-3

Circular dovetail attachment

Interchangeable

Variable inclination angles: 5 - 35 degrees

Eyepiece WHB10X, WHB10X-H (F.N. 20)

WH10X, WH10XH, 35WH10X, PWH10X (F.N.22)

9Power supply Continuous intensity adjustment

Built-in voltage changeover switch (100/120V, 220/240V), 0.45A

Frequency 50/60Hz, halogen bulb: 6V30W

10 Weight 7.0kg 7.3kg

CKX31/CKX41  A. OUTLINEOFPRODUCT

absorbing filter(45HA) be used because appropriate exposure can be obtained.

10X (F.N.20)

A-3 2

5. Dimensions

364

370.5

486.6

194.5

380.5

473.2

364

370.5

515.5

195

393.1

473.7

1) CKX31

1) CKX41

UNIT: mm

237.6

CKX31/CKX41  A. OUTLINEOF PRODUCT

A-4

U-CTR30-2U-CBI30-2CKX-TBIU-TBI3U-BI30-2U-TR30-2

BX2TVSystem

CKX-RCD

(w/slider&ringslit)

IX2-SLP

(w/PHP)

UPLFL4XPHP

IX2-SL

(w/PHL)

CACH10XPHP

LCACH20XPHP

LCACH40XPHP

IX2-SLPH1

IX2-SLPHC

IX2-SLPH2

IX2-BCTP

CK2-SS

CK40-MVR

PLC4X

PLC10X

ACH20X

LCPLFL40X

UPLFL4XPH(PHL)

CPL10XPH(PHC)/PL10XPH(PH1)

LCACH20XPH(PHC)/LCPLFL20XPH(PH1)

LCACH40X2PH(PH2)

CPL10XRC

LCACH20XRC

LCACH40XRC

45KB-3-L

U-RFLT50

32ND25

CKX-RFA

(w/B,G)

WH10X/WH10X-H

35WH10X/PWH10X

U-CT30

WHB10X/CT-5

WHB10X-H

CK40-CPG

CK40-CPG30

IX2-GCP

CKX41SF

w/U-LS30-3-2

CKX31SF

:Newunit(primary)

:Newunit(second)

LUCPLFL40XRC

*Others：objectivesapplicable

U-LH50HG

CKX-NU

w/U-LS30-3-2

6V30WHAL

*InU-excitation,

removetheUVcutfilter.

*ForU-excitation

(w/WHB10X

WHB10X-H)

43IF550W45

43ND25-W45

45LBD-2N

toPHL,PHC,PH1,PH2

equivalentto

WHC10X

CKX31/CKX41 A. OUTLINE OF PRODUCT

A-5

6. CKX31 / CKX41 system chart

Part

1. Inspection items and Methods

<CKX31>

Item Standard Method

Binocular

tube Interpupillary

distance adjustment 48mm - 75mm In the observation state, insert a thin sheet of a

paper with graduations at the eye point position and

measure the interpupillary distance.

Interpupillary

distance working force (500g - 1500gf)

5N - 15N

0.1mm or less on the image

surface

Put a string on the sleeve and measure the working

force to actuate the interpupillary distance using

a tension gauge.

Using the standard eyepiece (KN0048; with

adapter-1) and an objective (PlanC10X),

observe a specimen whose center can be identified

(ex. concentric circles) on the stage.

By moving the stage, match the specimen center

and the visual field center in the right sleeve.

range

Revoving axis

Open and close the interpupillary distance, and

read the movement of the image using the reticle

scale (1 graduation = 0.1mm) of KN0048.

Left / right optical axis On image surface:

0.2mm or less in vertical

direction

0.2mm or less in outward

direction

0.4mm or less in inward

direction

Using the standard eyepiece (KN0048; with

adapter-1) and an objective (PlanC10X),

observe a specimen whose center can be identified

(ex. concentric circles) on the stage.

Taking the right sleeve as the reference, read the

displacement between the specimen center and

the visual field center in the left sleeve using

the reticle scale (1 graduation = 0.1mm) of the

KN0048.

Absolute optical axis 0.1mm or less on the image

surface Combining the standard eyepiece (KN0048; with

Adapter-1), a microscope frame (product), the

standard objective (KN0041), read the

displacement between specimen center in KN0041

and the visual field center in the right sleeve

using the reticle scale (1 graduation = 0.1mm) of

the KN0048.

Exit pupil center Within 30% of objective’s

exit pupil diameter Combining the centering telescope (KN0029),

an objective (PlanC10X), and microscope frame

(product), read the displacement between the exit

pupil center of the objective and cross hairs center

Pafocality + / - 0.3mm Combine the standard eyepiece (KN0048; with

adapter-1, the focusing telescope (FT-36),

the microscope frame (Product), the standard

objective (KN0041).

In the left sleeve, focus on the specimen in KN0041

and check the parfocality using the helicoid scale

(1 graduation = 0.1mm) of the KN0048.

* 1) Since the binocular section of CKX31 is the same as that of CK30, the procedure of optical axis adjustment is

performed in the same manner as CK30. (Refer to the adjustment part of D-3 - D-14 in CK30/40 repair manual.)

2) Nesessary jigs are partially different because UIS optical system is adopted in CKX. (Refer to the above description.)

3) Diffrence between CK30 and CKX31 in parfocality adjustment: CK30 uses the spacer (selection : 3pcs. available),

While CKX31 uses the washer ( selection: 6 pcs. available) when adjusted and it requires more precise standard.

of centering telescope (KN0029).

B-1

CKX31/CKX41  B. INSPECTIONSTANDARD

(center of standard

objective)

Tension gauge (30N): OT3223

Part Item Standard Method

Coarse / fine

focus adjustment knob

knob

6N - 25N ( 600g - 2500gf) Tie a string around the knob and then

measure the tension when the knob

is rotated.

Tension gauge (30N): OT3223

adjustment

Coarse focus

Fine focus

adjustment knob

tension

0.3N - 1.2N ( 30g - 120gf) Tie a string around the knob and then

measure the tension when the knob

is rotated.

Tension gauge (3N): OT3225

Coarse / fine

adjustment knob

movement

Confirm that there is no rough feeling.

Focusing guide

unit Working force 0.2N - 0.5N ( 20g - 50gf)

difference

Working force

Confirm the working force while

pushing inner guide.

Tension gauge (3N): OT3225

in moving range

0.2N (20gf) Confirm the working force difference

in moving range.

Movement Cashing or roller should not be stopped.

There is no play between the inner

guide and the outer guide.

Stage Squareness against

optical axis 0.3/75mm Set the SKN0003 to the revolving

nosepiece and confirm the front-back,

left-right squareness of the stage.

(Not go through 0.3mm thickness)

Thickness gauge: OT1949

Part Item Standard Method

Exit pupil center Within 10% of objective’s

exit pupil diameter Combining the centering telescope (KN0023),

an objective (PlanC10X), UIS observation tube

(BXKN001) and microscope frame (product),

read the displacement between the exit pupil

center of the objective and cross hairs center

of centering telescope (KN0023).

Pafocality + / - 0.3mm Combine the standard eyepiece (KN0048; with

adapter-1and adapter-2 ), 3mm ring adapter

(KC2040), the focusing telescope (FT-36),

the standard objective(KN0041), BXKN001

and microscope frame (product).

Focus on the specimen in KN0041 and check

the parfocality using the helicoid scale

(1 graduation = 0.1mm) of the KN0048.

Absolute optical axis Combine the standard eyepiece (KN0048; with

adapter-1and adapter-2 ), 3mm ring adapter

Frame

section

BXKN001 and microscope frame (product).

Read the displacement between specimen center

in KN0041 and the visual field center using

the reticle scale (1 graduation = 0.1mm) of

KN0048.

0.1mm or less on the image

surface

* In parfocality adjusument, the washer (selection: 6 pcs. available) is used for CKX41 (same as CKX31).

B-2

CKX31/CKX41 B. INSPECTIONSTANDARD

tension

<CKX41>

(KC2040), the standard objective (KN0041),

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