Leica DMI6000 B Bedienungsanleitung

Leica DMI6000 B

Operating Manual

Published October 2004 by:

Leica Microsystems Wetzlar GmbH

Ernst-Leitz-Straße

D-35578 Wetzlar (Germany)

Responsible for contents:

Bernard Kleine

(Marketing CM, Compound Microscopy, Product Manage-

ment)

Holger Grasse

(Safety Officer according to MPG §30) In case of questions, please contact the hotline:

Phone +49(0)6441-292286

Fax +49(0)6441-292255

E-mail: [email protected]

Leica DMI6000 B

Operating Manual

Copyrights

All rights to this documentation are held by

Leica Microsystems Wetzlar GmbH. Reproduc-

tion of text or illustrations (in whole or in part) by

print, photocopy, microfilm or other method (in-

cluding electronic systems) is not allowed with-

out express written permission from Leica Mi-

crosystems Wetzlar GmbH.

The term "Windows" may appear in the following

text without further identification. It is, however,

a registered trademark of Microsoft Corpora-

tion. The names of companies and products

used herein may be trademarks of their respec-

tive owners.

The instructions contained in the following doc-

umentation reflect state-of-the-art technology

and knowledge standards. We have compiled

the texts and illustrations as accurately as pos-

sible. Nevertheless, no liability of any kind may

be assumed for the accuracy of this manual’s

contents. Still, we are always grateful for com-

ments and suggestions regarding potential mis-

takes within this documentation.

The information in this manual is subject to modifi-

cation at any time and without notification.

Contents

6.11 Installation of Lamp Housing Mount

and Mirror Housing ................................... 42

6.12 Installation and Replacement

of Incident Light Lamps ............................ 44

6.13 Equipping the Incident Light

Turret Disk ................................................... 48

6.14 Installation of the Polarizer and

Analyzer....................................................... 50

6.15 Optional Accessories ............................... 52

6.16 Connection to the

Electronics Box CTR6000 ......................... 53

6.17 Connection to the Computer ................... 54

6.18 Connection to the Power Supply ............ 54

7. Start-up........................................................ 55

7.1 Functional Principle .................................. 55

7.2 Switching on the Microscope................. 59

7.3 The LeicaScreen........................................ 60

7.4 The Function Buttons on the Stand ....... 61

7.5 The SmartMove Remote Control

Module......................................................... 64

7.6 Illumination ................................................. 65

7.6.1 Transmitted Light ............................ 65

7.6.2 Incident Light - Fluorescence ...... 68

7.7 Checking Phase Contrast Rings ............. 69

7.8 Setting the Motorized Polarizer............. 70

7.9 Adjusting the Light Sources .................... 71

Contents

1. Important Notes about this Manual ...... 7

2. Intended Purpose of the Microscope ... 8

3. Safety Notes ............................................... 9

3.1 General Safety Notes ............................... 9

3.2 Electrical Safety ........................................ 10

4. Overview of the Leica DMI

6000 B......... 12

4.1 Specifications ............................................ 12

4.2 Glossary....................................................... 16

5. Unpacking the Microscope .................... 22

6. Assembling the Microscope .................. 25

6.1 Assembly Tools .......................................... 25

6.2 Installation of the Transmitted Light

Illumination Carrier (DL) ........................... 26

6.3 Installation of the DIC Module

and DIC Objective Prisms ........................ 27

6.4 Installation of Specimen Stages............. 28

6.5 Installation of Condensers....................... 34

6.6 Installation of Eyepieces.......................... 39

6.7 Installation of Objectives ......................... 39

6.8 Installation of Filters

in the Illumination Arm ............................. 40

6.9 Installation of the

Transmitted Light Lamp Housing ............ 40

6.10 Installation and Replacement of the

Transmitted Light Lamps: .........................

Lamp Housing 107 or 107/2 ...................... 41

Contents

8. Operation .................................................... 74

8.1 Switching On .............................................. 74

8.2. Contrast Methods...................................... 76

8.2.1 Brightfield (TL)................................. 76

8.2.2 Phase Contrast (TL) ........................ 77

8.2.3 Darkfield (TL) ................................... 77

8.2.4 Polarization (TL) .............................. 78

8.2.5 Differential

Interference Contrast (TL) ............ 79

8.3 Fluorescence.............................................. 80

8.4 Combination Methods .............................. 81

8.5 Focusing ...................................................... 82

8.6 Tubes............................................................ 84

8.7 Eyepieces .................................................... 85

8.8 Objectives ................................................... 85

8.9 Stages and Object Displacement ........... 88

8.10 Magnification Changer............................. 89

8.11 Light Sources ............................................. 89

8.12 Aperture and

Field Diaphragm ......................................... 90

9. Troubleshooting......................................... 91

10. Care of the Microscope ........................... 95

10.1 Dust Cover .................................................. 95

10.2 Cleaning....................................................... 95

10.3 Handling Acids and Bases ...................... 96

11. Major Consumable and Replacement

Parts ............................................................ 97

12. Dimensions................................................. 98

13. Abbreviations and Pictograms............... 99

14. Index ............................................................ 101

15. EU Declaration of Conformity ................. 104

1. Important Notes about this Manual

(1.2)

→

p.20

Numbers in parentheses, such as "(1.2)", corre-

spond to illustrations (in the example, Figure 1,

Item 2).

Numbers with pointer arrows (for example

→p.20), point to a certain page of this manual.

Caution!

Special safety instructions within this manu-

al are indicated with the triangle symbol

shown here, and have a gray background.

Caution! The microscope and accessories can

be damaged when operated incorrectly.

Explanatory note.

Item not contained in all configurations.

Text symbols, pictograms and their meanings:

Caution!

This operating manual is an essential com-

ponent of the microscope, and must be read

carefully before the microscope is assem-

bled, put into operation or used.

1. Important Notes about this Manual

This operating manual contains important in-

structions and information for the operational

safety and maintenance of the microscope and

accessories. It must therefore be kept safely for

future reference.

A separate manual is available on CD-ROM cov-

ering the operation of the Leica Application

Suite (LAS).

2. Intended Purpose of the Microscope

The Leica DMI

6000 B microscope described in

these instructions is intended for biological rou-

tine and research applications. This includes the

examination of samples taken from the human

body to provide information on physiological or

pathological states or congenital abnormalities,

or determing the safety and compatibility with po-

tential recipients, or monitoring therapeutic

measures.

The Leica DMI

6000 B is a further development

of Leica’s proven inverted research micro-

scopes. It is designed for cellular and tissue ex-

amination, micromanipulation and microinjec-

tion techniques, microdissection, and confocal

microscopy. The Leica DMI

6000 B is suitable for

universal deployment, all contrast methods such

as darkfield, brightfield, phase contrast, DIC, flu-

orescence, and modulation contrast are integral

to the microscope and can be adapted or

changed quickly and easily. Variable illumination

and imaging beam paths, as well as HCS optics,

modular accessories and a comprehensive

range of peripherals, complement the Leica DMI

6000 B inverted research stand.

The above-named microscope complies with

the Council Directive 98/79/EEC concerning in

vitro diagnostics. It also conforms to the Council

Directives 73/23/EEC concerning electrical ap-

paratus and 89/336/EEC concerning electromag-

netic compatibility for use in an industrial envi-

ronment.

Caution!

The manufacturer assumes no liability for

damage caused by, or any risks arising from

using the microscope for other purposes

than those for which it is intended or not us-

ing it within the specifications of Leica Mi-

crosystems Wetzlar GmbH.

In such cases the declaration of conformity

shall cease to be valid.

Caution!

This (IVD) device is not intended for use in

the patient environment defined by DIN VDE

0100-710. Neither is it intended for combin-

ing with medical instruments according to

EN 60601-1. If a microscope is electrically

connected to a medical instrument accord-

ing to EN 60601-1, the requirements de-

fined in EN 60601-1-1 shall apply.

3. Safety Notes

3.1 General Safety Notes

This safety class 1 device is constructed and

tested in accordance with

EN 61010-2-101:2002,

EN 61010-1:2001,

IEC 1010-1:2001,

Safety regulations for electrical measuring, con-

trol, and laboratory devices.

Caution!

In order to maintain this condition and to en-

sure safe operation, the user must follow the

instructions and warnings contained in this

operating manual.

Caution!

The devices and accessories described in

this operating manual have been tested for

safety and potential hazards.

The responsible Leica affiliate or the main

plant in Wetzlar must be consulted whenev-

er the device is altered, modified, or used in

conjunction with non-Leica components that

are outside of the scope of this manual.

Unauthorized alterations to the device or

noncompliant use shall void all rights to any

warranty claims!

3. Safety Notes

Caution!

Power plugs may only be plugged into an

outlet equipped with a grounding contact.

Do not interfere with the grounding function

by using an extension cord without a ground

wire. Any interruption of the ground wire in-

side or outside of the device, or release of

the ground wire connection, can cause the

device to become hazardous. Intentional

ground interruption is not permitted!

Caution!

Peripheral devices with their own or sepa-

rate power supplies that are connected to

the microscope can have the same protec-

tive conductor potential by connecting them

to the ground screw on the back of the Leica

CTR6000 electronics box. For connections

without a ground connector, Leica Service

must be consulted.

Caution!

Never use any fuses as replacements other

than those of the types and the current rat-

ings listed here. Using patched fuses or

bridging the fuse holder is not permitted. The

use of incorrect fuses may result in a fire

hazard.

90-250V~

50-60 Hz

max. 290VA

T6.3 A

(IEC 60127-2/3)

15-35°C

max. 80% to 30°C

90-250V~

50-60 Hz

See CTR6000

15-35°C

max. 80% to 30°C

90-250V~

50-60 Hz

max. 155VA

2xT2A (IEC 127)

10-36°C

max. 80% to 30°C

3.2 Electrical Safety

General specifications

Leica CTR6000 Electronics Box

For indoor use only.

Supply voltage:

Frequency:

Power input:

Fuses:

Ambient temperature:

Relative humidity:

Overvoltage category:

Pollution degree:

Microscope

For indoor use only.

Supply voltage:

Frequency:

Power input:

Fuses:

Ambient temperature:

Relative humidity:

Overvoltage category:

Pollution degree:

ebq 100 Supply Unit*

For indoor use only.

Supply voltage:

Frequency:

Power input:

Fuses:

Ambient temperature:

Relative humidity:

Overvoltage category:

Pollution degree:

(See enclosed manual)

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