Olympus IMT-2 Bedienungsanleitung

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INVERTED RESEARCH

MICROSCOPE

This instruction manual has been written for use of the

Olympus lnverted Research Microscope Model IMT-2. Before

putting the microscope into operation, it is recommended

that you read this manual carefully in order to familiarize

yourself fully with the use of this microscope so that you

may obtain optimum performance of this instrument.

----

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IBEFOREUSE

Observe the following points carefully for operation and maintenance:

Operation

@ As a microscope is a precision instrument, always handle

it with the care it deserves, and avoid abrupt motions

and shocks.

To lift the microscope from the packing case, hold it

at the OM mount (A) and the fluorescence illuminator

mounting thread (B). (Fig. 1)

To move the microscope

Hold the microscope by the OM mount (A) and the

fluorescence illuminator mounting thread (B) or place

your hands under the microscope base at the microscope

side (C) and back (D).

Do not grasp the plastic hand rests, since they are not

strong enough to support the microscope.

Do not use any bulb other than the one designated by

Olympus (12V50WHAL halogen bulb).

Avoid exposure of the microscope to direct sunlight, (Model IMT2SFR shown)

high temperature and humidity, dust and vibration.

Make it a point to use the tension adjustment ring to adjust the tension of the coarse adjustment knobs. (

not rotate the coarse adjustment knobs in the opposite directions simultaneously.)

Make sure that the line voltage selector switch is set to conform with the local mains voltage.

Disconnect the power cord from the AC outlet before fuse replacement.

Always ground the microscope.

Maintenance

:Do

@ Lens surfaces must always be kept clean. Fine dust on lens surfaces should be blown off by means of a hand

blower. Carefully wipe off oil or fingerprints on the lens surfaces with gauze moistened with a small amount

of xylene, or a cleaning medium (alcohol and ether 3 : 7).

@ Do not use organic solutions to wipe the surfaces of various plastic components.

@ Be careful not to spill the culture solution, etc. If spilt, it should be wiped off immediately. It is recommended

to use a waterproof cover optionally available.

@ When objectives are not screwed into the nosepiece apertures, seal the apertures with dust plugs, which will

protect the lenses located in the lower light path from dust, culture solutron, etc.

@ After use the microscope should be covered with the vinyl dust cover provided and stored in a place free from

humidity.

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5-l Observation Procedure ............................. 10

5-2 Operation of Individual Components .................. 11

6-l Photomicrographic System .......................... 18

6-2 Taking Pictures ................................... 20

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Itern

vlicroscope

Specifications

Focus adjustment by vertical movement of the nosepiece (stage is fixed in position) by means

#sand of the coaxial coarse and fine adjustment knobs, roller guide stroke (from the focal point on

the stage surface). 8mm upward and 2mm downward; with reduction gear, graduated rn tncre-

ments of 2g.

\Josepiece. Sextuple, with provision for mountrng the Nomarski slrder attachment, detachable.

Gtage: Cross-movement stage

IMT2SVR

Square plain stage

Mechanical stage

attachment IMT2-MVR

Ilumination: Light source

Filter holder

Condenser holder

200mm x 200 mm, traversing area 50mm x 50 mm; with low posi-

tioned coaxial control knobs; 2 insert plates, outside diameter 1lOmm,

inside diameters 20 mm and 50 mm respectively.

200mm x 2OOmm, traversing area 50mm x 50 mm; with low posi-

tioned coaxial control knobs; 2 insert plates, outside diameter 1IOmm,

inside diameters 20 mm and 50 mm respectively, low flexible.

160 mm x 220 mm; insert plate, outside diameter 110 mm. Substage

provided; provision for attaching the mechanical stage IMT2-MVR.

Traversing area 110mm x 72mm; attachable at the right or left side

on the plain stage.

Provision for accommodation of various culture vessels and specimen

holders.

Halogen bulb 12V50WHAL, with bulb centering device and light in-

tensive control; Voltage indication by bar graph voltmeter.

Provided with 4 flip-up filter holders; green interference filter and

frosted filter.

Flip-up, swing-out type; circular mounting dovetail for slide-in con-

denser; vertical movement on rack-and-pinion, condenser centering

knobs; tension adjustment of the height adjustment knob.

Observation

system. Built-in magnification changer, with centering telescope 1X-1 .5X-CT.

Light path for photomicrography, 3-setting positions, linked with focusing retitles; for observa-

tion tube (BI), 35mm camera (OM) and multi-tube mounting port (MTU).

Binocular tube, inclined 45’; interpupillary distance adjustment from 53 mm to 75mm; constant

tube length adjustment.

Eyepieces WHKlOX, WHKIOX-H (field number 20)

OM light path: 2.5X FK photo eyeprece built-in; OM system bayonet mount.

Multi-tube light path; provision for mounting the PM-10 photomicrographic equipment directly

as well as the multi-tube attachment.

Condenser.

Objectives.

Long working distance turret condenser; N.A. 0.55, W.D. 21 mm, light annuli for 4X, 10X,

20X and 40X objectives and empty aperture; aperture iris diaphragm.

Ultra long working distance condenser, N.A. 0.30, W D. 55mm, Light annul1 for 4X, 10X,

20X, and 40X objectives plus empty aperture; aperture Iris diaphragm.

Phase contrast objectives PC S Plan 4XPL N.A. 0.13, W.D 15.5 mm

PC S Plan 1OXPL N.A. 0.30,*W.D. 7.5mm

LWD-CD Plan 20XPL N.A. 0.40, W.D. 3.0 mm

with correction collar

LWD-CD Plan 4OXPL N.A. 0.60, W.D. 1.9 mm

wrth correction collar

Dimensions: 320 mm (W) x 395mm (D) x 600 mm (H) Eyepoint 405 mm Stage height 275 mm

Weight: 20.5 kg (outfitted with standard equipment)

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Component

Microscope stand,

with built-in magnification changer (1X-l .5X-CT),

built-in 2.5X photo eyepiece, 50W halogen illumi-

nator, condenser holder, hand rests (paired), dust

cover, filter 431F550-W45 and screw driver

Power cord

Binocular tube, 45’ inclined

Intermediate tube

;extuple revolving nosepiece

jOW halogen lamp housing

jOW halogen bulbs, 2 PCS.

Iross-movement mechanical stage with right-hand

ow drive controls

:ross-movement mechanical stage with right-hand

ow flexible

Model IMT-2-l 1 IMT-2-l 2

IMT2-F 0 0

UYCP 0 0

BH2-B145 0 0

IMT2-ATU 0 0

IMT2-RE 0 0

IMT2-LSH 0 0

JC12V50WHAL 0 0

IMT2-SVR

;quare plain stage

nechanical stage attachment with low drive controls

-ong working distance turret condenser

Jltra long working distance turret condenser

Objectives

Eyepieces

IMT2-SFR

IMT2-SP2 0 0

IMT2-MVR 0 0

IMT2-LWCD 0

IMT2-U LWCD 0 0

PCSPL4XPL 0

PCSPLlOXPL 0 0

LWDCDPL2OXPL 0

LWDCDPL40XPL

I::::::-, / : 1 i

Note: 0 indicates the compatible components for Model IMT-2-1 l/ IMT-2-12/lMT-2-21/IMT-2-31.

vlT-2-21 IMT-2-31

0 I 0

O I 0

0 I 0

0 0

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Field iris diaphragm

lever

Lamp housing Long working distance

condenser

Stage insert plate

omarski slider

insertion slot %\

insertion slot

Exciter filter

Multi-tube mounting

-I~ ~.

aaapTer

Main switch

(IMT-2-12 shown)

\Eyepiece

Diopter adjustment

Observation tube

ring

Line voltage selector

switch

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Lamp housing

\ Bulb horizontal centering

Bulb socket clamping/

ong working distance

condenser

---.

Condenser height

Condenser holder

clamoina knob

Stage cross-movement

knobs

Light excluding shutter kno

Light path selector knot

BI : for binocular tub{

OM : fboa;;5mrn camen

. I

\ Minimum line voltage

Tension adjustment knob ‘/ / /Frne adjustment knob 1 Hand resr\ se’ector knob

Coarse adjustment knob

Light intensity

control lever

( IMT-2-12 shown)

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Remove the adhesive tape that locks the light path selector knob in transit. The diagram below illustrates the

sequential procedure of assembly. The numbers indicate the assembly order of various components.

It Remove dust caps before mounting components.

CD Eyepiece

(i) Long working

distance condenser 0’ Ultra long working

distance condenser

0 Socket and

cord assembly 0 Halogen bulb @ Filter

Lamp housing 4

Stage clip k @ Objective

!!3

+ I

0 Cross-move- 11

0’ Stage insert plate

0’ Plain stage +

I 0 o’y, ~

n. I. R 4 A

ment stage

ii! I

@35mm camera

back

-1

@Adapter for

photomicrographic

attachment

@“Extension plate L

lr - Allen@ wrench

0” Mechanical stage

attachment

Cm Hand rest )

m Pnrnmrr.r\rA

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Fig. 2

L-1 q

Fig. 3Fig. 3

Fig. 4

Mounting the Stage

) Loosen the clamping screw 0. (Fig. 2)

) Aligning the stage positioning groove to the positioning pin 0, place

the circular mounting dovetail of the stage on the stage holder 0.

(Fig. 2)

) As the stage clamping screw is tightened, both ends of the stage will

be fastened tightly to the microscope stand.

0 Mounting the Objectives

1) Loosen the condenser holder clamping knob 0, and flip up the

condenser holder 0. (Fig. 3) >

* The tension

of the condenser holder tilting motian can be ad- E

justed by means of the clamping screw @ which can be rotated a

with a coin. P

2) Screw each objective @ into the nosepiece @ through the stage i

insert mounting hole @ in the stage, lower power to higher power,

in a clockwise direction. (Fig. 4)

3) Click the condenser holder back to the original positon, and clamp

with clamping knob.

* Please note that

the front lens of the objective in the inverted

microscope faces upward, and is exposed to contamination more

than the objective of an upright microscope. Therefore, if there

are empty openings in the nosepiece, use the dust plugs 0 pro-

vided to prevent dust or debris from falling into the prisms, etc.

0 Mounting the Cross-movement Stage

1) Stage insert plate

Place a stage insert plate @ on the stage. (The insert plates are

provided with inner diameters 20 mm and 50 mm. It is recom-

mended to select a stage insert plate with an inner diameter slightly

smaller than that of a culture vessel in order to prevent the vessel

from toppling down.) (Fig. 5)

* The insert plate is designed to be very thin so as to avoid hitting

against the objectives at objective magnification change.

Ir Do not handle the insert plate roughly since this may deform.

2) Stage clip

Attach a pair of stage clips provided with clamping screws @ on the

stage.

Fig. 5

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