Olympus CKX53 Bedienungsanleitung

Instructions

Notes

This instruction manual is for the Olympus culture microscope model CKX53.

To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of the

microscope, we recommend that you study this manual thoroughly before operating the microscope,

and always keep this manual at hand when operating the microscope.

Retain this instruction manual in an easily accessible place near the work desk for future reference.

For details of products included in the configuration of this system, see page 7.

CKX53

A X 8 6 6 0

Culture Microscope

Optical microscope and accessory

In accordance with European Directive on Waste Electrical and Electronic Equipment, this

symbol indicates that the product must not be disposed of as unsorted municipal waste, but

should be collected separately.

Refer to your local Olympus distributor in EU for return and/or collection systems available in

your country.

NOTE: This product has been tested and found to comply with the limits for a Class A digital device,

pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection

against harmful interference when the product is operated in a commercial environment. This product

generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance

with the instruction manual, may cause harmful interference to radio communications.

Operation of this product in a residential area is likely to cause harmful interference in which case the

user will be required to correct the interference at his own expense.

FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance

could void the user’s authority to operate the product.

For Korea only

B급 기기 (가정용 방송통신기자재)

이 기기는 가정용(B급) 전자파적합기기로서 주로 가정에서 사용하는 것을

목적으로 하며, 모든 지역에서 사용할 수 있습니다.

Introduction....................................................................................................................................................... 1

Safety precautions...................................................................................................................................... 2

1 Nomenclature of units........................................................................................................................ 7

2 Nomenclature of main operating portions....................................................................... 8

2-1 Bright field set / Phase contrast entry set / Phase contrast standard set..... 8

2-2 Fluorescence set............................................................................................................................................... 9

3 Observation procedures.................................................................................................................10

3-1 Preparation of observation .....................................................................................................................10

1Moving the microscope........................................................................................................................................................10

2Attaching AC adapter and power cord................................................................................................................. 10

3Cleaning and sterilizing around the stage.......................................................................................................... 11

3-2 Procedures to place specimens.......................................................................................................11

1Placing the container on the stage............................................................................................................................ 11

2When using the optional stage unit.......................................................................................................................... 11

3When using the high container (Procedures to attach / detach the condenser portion)........................12

3-3 Observation methods (Bright field observation / Phase contrast observation)............12

1Turning ON the light source (LED)............................................................................................................................... 12

2Changing between bright field observation and phase contrast observation

(Phase contrast entry set / Phase contrast standard set / Fluorescence set)................... 12

3Adjusting the aperture iris diaphragm..................................................................................................................... 13

4Selecting the objective.......................................................................................................................................................... 13

5Changing between the visual light path and the camera light path........................................... 13

6Focusing on the specimen................................................................................................................................................ 14

7Adjusting the brightness...................................................................................................................................................... 14

8Moving the specimen ........................................................................................................................................................... 14

Contents

3-4 Observation method (Fluorescence observation)..............................................................15

1Inserting the shutter for reflected light illumination (Fluorescence set)....................................15

2Inserting the umbra shield (ambient light shielding plate) (Fluorescence set)...................15

3Turning ON the light source (mercury burner) (Fluorescence set)................................................. 15

4Inserting the fluorescence mirror unit (Fluorescence set)..................................................................... 16

5Changing between the visual light path and the camera light path...........................................16

6Selecting the objective..........................................................................................................................................................16

7Opening the shutter for reflected light illumination (Fluorescence set).................................16

8Focusing on the specimen................................................................................................................................................16

9Moving the specimen............................................................................................................................................................. 16

3-5 Recording with the camera....................................................................................................................17

1Changing between the visual light path and the camera light path........................................... 17

2Focusing on the specimen................................................................................................................................................ 17

3Acquiring the image ................................................................................................................................................................ 17

3-6 Cleaning up..........................................................................................................................................................18

1Cleaning up the fluorescence illuminator (Fluorescence set)...........................................................18

2Turning OFF the light source (mercury burner) (Fluorescence set)............................................... 18

3Turning OFF the light source (LED)

(Bright field set / Phase contrast entry set / Phase contrast standard set)..........................18

4Removing the AC adapter and the power cord .............................................................................................18

5Cleaning up the microscope...........................................................................................................................................18

4 For advanced observation ..........................................................................................................19

4-1 Adjusting the binocular portion..........................................................................................................19

1Adjusting the interpupillary distance........................................................................................................................ 19

2Adjusting the diopter...............................................................................................................................................................19

3Using the eye shades............................................................................................................................................................19

4-2 Adjusting the tension of the coarse focusing knob........................................................20

4-3 Operating procedures of the transmitted light illumination filter (option)....20

4-4 Adjusting the umbra shield (ambient light shielding plate) position

(Fluorescence set)..........................................................................................................................................20

4-5 Adjusting the field iris diaphragm in fluorescence observation (Fluorescence set).... 21

4-6 Observing with the objective with correction collar (option)................................... 21

4-7 Centering of the centering phase contrast sider IX2-SL (option) .......................22

4-8 Operating procedures of PLN2X and CKX3-SLPAS (option)....................................22

4-9 Operating procedures of CKX3-SLPIC (option)...................................................................23

4-10 Selecting procedures of the specimen holder (option) ............................................ 23

4-11 Selecting procedures of the fluorescence mirror unit (including options)...24

1Selecting the fluorescence mirror unit (Fluorescence set)...................................................................24

2Spectral characteristics of the filter of the fluorescence mirror unit (Fluorescence set) ........24

5 Troubleshooting....................................................................................................................................25

5-1 Bright field observation / Phase contrast observation .................................................25

5-2 Fluorescence observation...................................................................................................................... 26

5-3 Common in all observation methods...........................................................................................27

6 Specifications..........................................................................................................................................28

6-1 Sets with CKX53.............................................................................................................................................28

6-2 Objective................................................................................................................................................................30

6-3 Optional units ....................................................................................................................................................30

6-4 Operating environment............................................................................................................................. 31

7 Assembly ...................................................................................................................................................32

7-1 Assembly diagram.........................................................................................................................................32

7-2 Assembly of the microscope frame CKX53SF.....................................................................33

7-3 Assembly of the fluorescence illuminator CKX3-RFA (Fluorescence set) .......34

7-4 Assembling the stage (Phase contrast standard set / Fluorescence set)......38

7-5 Attaching the objectives...........................................................................................................................39

7-6 Attaching the phase contrast slider

(Phase contrast entry set / Phase contrast standard set).......................................... 40

7-7 Attaching the filter slider for transmitted light illumination ........................................ 41

7-8 Attaching the light source for reflected light illumination (Fluorescence set)........ 41

7-9 Attaching the camera..................................................................................................................................45

8 Preventive inspection sheet for illumination devices

(Fluorescence set) .............................................................................................................................46

9 Appendix..................................................................................................................................................... 47

9-1 Summary of procedures of bright field observation / phase contrast observation....... 47

9-2 Summary of fluorescence observation procedures........................................................ 48

CKX53

Configuration of instruction manuals

Read all the instruction manuals supplied with the units you purchased.

The following instruction manuals are prepared for this product.

Instruction manuals Main contents

CKX53 (this document) Operation procedures of the microscope, etc.

Mercury power supply unit

U-RFL-T Power supply unit of mercury lamp housing

Light guide illumination system

U-HGLGPS Light guide illumination system

Introduction

Intended use

This product has been designed to be used to observe magnified images of specimens in various routine work and

research applications.

This includes the observation of living cells or of specimen taken from tissues to gain physiological or morphological

information at hospitals or laboratories. Typical field of applications are genetics, human blood and tissue examination,

neurology, pharmacology and cellular biology.

Do not use this instrument for any purpose other than its intended use.

This product complies with the requirements of directive 98/79/EC concerning in vitro

diagnostic medical devices. CE marking means the conformity to the directive.

This product is applied with the requirements of EMC standard IEC/EN61326-2-6 and

IEC/EN61326-1 concerning electromagnetic compatibility.

This product complies with the emission and immunity requirements described in IEC61326

series. The electromagnetic environment should be evaluated prior to operation of this product.

If the product is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the

product may also be damaged. Always use this product according to the instruction manual.

The following symbols are used in this instruction manual.

CAUTION : Indicates a potentially hazardous situation which, if not avoided, may result in

minor or moderate injury.

NOTE

: Indicates a potentially hazardous situation which, if not avoided, may result in

damage to the product or other property.

TIP

: Indicates the useful knowledge or information for use.

CAUTION - Prevention of infection -

CAUTION - Installation of the product -

If you observe specimens which have potentiality of infection, please comply with following items to

prevent from infection.

• Wear protective equipment such as gloves, etc.

Wear protective equipment such as gloves, etc. to prevent the specimens from touching your skin directly.

When you maintain the product which may have contacted the specimens, wear protective equipment such as

gloves, etc., or clean the product before operation.

• Follow the procedures described in chapter "Handling Precautions ".

Follow the procedures described in chapter "Handling Precautions" (page 5) prior to using the units of this product.

Otherwise, the stability of this product will be lost and the dropped sample will cause the possibility of infection.

• After observation, clean the portions where specimens contacted directly.

• Remove the specimen when moving the product.

When moving this product, be sure to remove the specimen first, since it is in danger of dropping and spattering.

• In case the specimen is damaged, promptly take the infection prevention measures.

• When disposing of the product, follow the regulations and rules of your local government.

When disposing of the product which contacted the specimens, be sure to follow the regulations and rules of your

local government.

• Follow the laboratory biosafety guidelines issued by WHO.

In addition, refer to the laboratory biosafety guidelines issued by WHO. This microscope is assumed to be used with

the biosafety level 3 or lower.

Install this product on a sturdy, level table or bench.

For safety in particular, do not place a mat, etc. under the product.

Safety precautions

CKX53

CAUTION - Illumination light (LED illumination light) for bright field

observation / phase contrast observation -

CAUTION - Electric safety -

Always use the AC adapter, the power cord and other cables provided by Olympus.

If other AC adapters, power cords, or cables are used, the electric safety and the EMC (Electro-Magnetic Compatibility)

performance of the product can not be assured. If no power cord is provided, please select the proper power cord by

referring to the section “Proper selection of the power cord” at the end of this instruction manual.

Always connect the ground terminal.

Connect the ground terminal of the power cord and that of the power outlet. If the product is not grounded, our

intended electric safety and EMC performance of the product can not be assured.

Do not use the product in close proximity to the sources of strong electromagnetic radiation.

Proper operation may be interfered. The electromagnetic environment should be evaluated prior to operation of the

product.

Do not touch the AC adapter, the power cord, or other cables or switches with wet hands.

If you touch them with wet hands, you may get an electric shock.

Remove the power cord in case of emergency.

In case of emergency, remove the power cord from the power cord connector on the product or from the power outlet.

Install the product at a location where you can reach the power cord connector or the power outlet at hand to remove

the power cord quickly.

This product complies with the emission and immunity requirements described in IEC61326 series.

CAUTION - Illumination light for fluorescence observation -

Do not look directly at the light coming out from the objective and the reflected light from the

specimen.

Attach the cap to the screw hole of the revolving nosepiece where the objective is not attached.

Engage the umbra shield (ambient light shielding plate) (see page 9) in the light path.

Be careful about the light emitted during the fluorescence observation, since not only visible light but also light of

invisible wavelengths (such as ultraviolet and infrared) may be emitted depending on the illumination methods.

Do not expose your skin to the light coming out from the objective for a long time.

Do not expose your skin to the light, since you may get burned.

Do not allow flammable gas or liquid to get close to the light coming out from the objective.

Do not allow flammable gas or liquid to get close to the light, since it may cause fire.

Do not look directly at the light from LED for a long time.

This product uses LED as a light source for the bright field observation and the phase contrast observation. This LED

is basically eye-safe. However, do not look directly at the light from LED for a long time, since it may cause damage to

your eyes.

The following symbols are placed on this product.

Study the meaning of the symbols and always use the product in the safest possible manner.

Symbol Meaning

Indicates high voltage (1kV or more). Take special care to prevent electric shock.

Indicates that the surface becomes hot, and should not be touched with bare hands.

It may cause burns.

Indicates a non-specific general hazard. Follow the description given after this symbol

or in the instruction manual.

Indicates the main switch of push button type.

(Push button type is the the type of switch that ON or OFF is selected each time it is pressed.)

Indicates that the seesaw type main switch is ON.

(Seesaw type is the type of switch that ON or OFF is selected by pressing it to ON or OFF side.)

Indicates that the seesaw type main switch is OFF.

Caution labels

The cautions are displayed on the portions which require special cautions during use and operation. Be sure to follow

these instructions.

Caution display

position

• Mercury lamp housing

U-LH100HG [ High voltage ] [ High temperature ]

• Power supply unit

U-RFL-T [ High voltage ] -

When caution labels are dirty or peeled off, contact Olympus for replacement or inquiries.

CAUTION - Safety symbols -

CAUTION - Mercury lamp housing -

Keep the AC adapter, the power cord and other cables well away from the mercury lamp housing.

If the AC adapter, the power cord and other cables contact a hot area of the lamp housing, they could melt and cause

electric shock.

Confirm that the mercury burner is attached properly and cords are connected properly.

Set the main switch to (OFF) when replacing the mercury burner.

To avoid electric shock hazards and burns when replacing the mercury burner, set the main switch to (OFF),

then remove the power cord from the product in advance. When you replace the mercury burner after use of the

microscope, wait until the mercury lamp housing and the burner are sufficiently cooled down.

Applicable burner : USH-103OL (Made by Ushio Inc.)

Secure sufficient space around the mercury lamp housing.

The top surface of the mercury lamp housing on the back of the microscope gets very hot. When attaching the

mercury lamp housing to the microscope, secure sufficient space around the mercury lamp housing, particularly

around the top surface and the bottom surface. Do not put any object on the top surface of the mercury lamp housing.

The product lifetime of the mercury lamp housing (not mercury burner) is 8 years or 20,000 illumination hours, whichever

occurs first, as a guideline. For details, see the preventive inspection sheet for illumination devices on page 46.

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