Zeiss Axiocam MR Installations- und Betriebsanleitung

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Number of this manual: B 48-0021 e

Date of issue: 03.2003

Date of the last revision/update: 10.2003

Carl Zeiss Vision draws the User's attention to the fact that the information and

references contained in these documents may be subject to technical modifications, in

particular due to the continuous further development of Carl Zeiss Vision's products.

The documents enclosed do not contain any warranty by Carl Zeiss Vision with regard

to the technical processes described in the documentation or to certain reproduced

product characteristics. Furthermore, Carl Zeiss Vision shall not be held liable for any

possible printing errors or other inaccuracies in this documentation, unless proof can

be furnished that any such errors or inaccuracies are already known by Carl Zeiss Vision

or that these are not known to Carl Zeiss Vision due to gross negligence and that

furthermore Carl Zeiss Vision has for these reasons refrained from eliminating these

errors or inaccuracies appropriately. Carl Zeiss Vision hereby explicitly draws the User's

attention to the fact that this manual only contains a general description of the

technical processes and information, the implementation of which in any individual

case may not be appropriate in the form described here. In cases of doubt, we

recommend the User to consult Carl Zeiss Vision.

This manual is protected by copyright. Carl Zeiss Vision has reserved all rights to this

documentation. It is prohibited to make copies, partial copies, or to translate this

manual into any other language. Carl Zeiss Vision explicitly draws attention to the fact

that the information contained in this manual will be updated regularly in compliance

with the technical modifications and supplements carried out in the products and

furthermore that this manual only reflects the technical status of Carl Zeiss Vision's

products at the time of printing.

Reproductions, copies, and even excerpts may only be made with explicit approval

from

Carl Zeiss Vision GmbH

Zeppelinstr. 4

85399 München-Hallbergmoos

http://www.zeiss.de

czv_support@zeiss.de

Microsoft, Windows, Windows 2000, Windows XP and Excel are registered trademarks

of Microsoft Corporation in the United States and/or other countries.

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Refer to the safety notes and instructions in the manuals of all

necessary devices (e.g. microscope peripherals, cameras,

computers, computer additionals, etc.) before installing and

using the software.

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27.10.2003 Installation adapted from Product CD 03.2003 to the installation of

the product CD number 15 of October 2003.

Table of Contents

AxioCam MR Installation + Reference i

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1Installation of the AxioCam MR ........................................................... 1

1.1 Safety Regulations....................................................................... 1

General Notes.......................................................................... 3

Backup Copies ......................................................................... 4

1.2 System Requirements .................................................................. 4

Contents of Delivery................................................................. 4

1.3 Setting up the Camera ................................................................ 5

Installing the PCI interface Board .............................................. 5

Connecting the Camera ........................................................... 6

Mounting the camera on the microscope ........................... 7

Connecting camera to PCI interface board.......................... 7

Power control .................................................................... 8

Extension connector........................................................... 8

Checking the Setup.................................................................. 8

1.4 Installing the Camera Driver ........................................................ 9

General.................................................................................... 9

Installing the Camera Driver with the Hardware Wizard .......... 10

Installing the Camera Driver with the Setup Program .............. 13

How to correct the driver entry in the device manager ...... 16

1.5 Notes on Maintenance .............................................................. 23

Camera Electronic.................................................................. 23

Optical System ....................................................................... 23

Cleaning the Infra-red Barrier Filter......................................... 23

Important Note on the Use of C-Mount Objectives ................. 23

2Image Acquisition with AxioCam MR ................................................ 24

2.1 General..................................................................................... 24

2.2 Quick Guide to the First Image with AxioVision and AxioCam MR25

The Workflow Standard ......................................................... 26

Step by Step to the First Image ............................................... 27

2.3 Adjust Property Page................................................................. 32

Exposure................................................................................ 33

Automatic exposure ......................................................... 34

White Balance........................................................................ 35

Color adjustment ............................................................. 36

Histogram.............................................................................. 36

Table of Contents

ii B 48-0021 e 10.2003

2.4 Frame Property Page ................................................................. 38

Camera Mode........................................................................ 38

Resolution of the AxioCam MR......................................... 39

Binning............................................................................ 41

Size of the resulting files .................................................. 41

Frame .................................................................................... 42

2.5 General Property Page............................................................... 43

Digital Gain............................................................................ 43

Image Orientation.................................................................. 44

Black Reference...................................................................... 44

White Reference .................................................................... 45

Shutter Control...................................................................... 46

Filter Operations..................................................................... 47

2.6 Practical Notes on Operation in AxioVision................................. 48

Speed of the live image.......................................................... 48

Notes on Optimum Color Reproduction in AxioVision ............. 49

2.7 Information for Displaying 12 Bit AxioCam Images in AxioVision 50

Recommended Camera Settings............................................. 52

Recommended Monitor Settings ............................................ 52

2.8 Background Information............................................................ 53

Gamma Setting...................................................................... 53

Linearity................................................................................. 53

Configurable Toolbars for Operating the Camera.................... 53

3Trouble Shooting ................................................................................ 54

3.1 Software................................................................................... 54

3.2 Image Quality ........................................................................... 55

4Technical Data ..................................................................................... 56

4.1 AxioCam MRc from Version 2.0 (Color Version) ......................... 56

4.2 AxioCam MRm from Version 2.0 (Black/White Version) .............. 58

4.3 Spectral Sensitivity..................................................................... 60

Installation of the AxioCam MR

AxioCam MR Installation + Reference 1

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The AxioCam MR is a professional, high-resolution digital camera. It has been

specially optimized for routine and research applications in the area of light

microscopy.

There are two versions available: AxioCam MRc (color camera) and AxioCam

MRm (b/w camera).

To make it easier for you to set up the camera, please follow the instructions

in these chapters step by step.

If the camera is connected to the computer by the data cable the LED on the

top of the camera is red, when the computer is started. After the first

initialization (starting the software) the color of the LED changes from red to

green. During continuous image acquisition the color of the LED changes

between red (exposure) and green (ready).

NOTE:

 In the description that follows the term AxioCam MR is used for both the

color camera and the black/white camera. A distinction is only made

where this is necessary for technical reasons.

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Please read this chapter carefully and observe the regulations in

order to ensure your safety and the intended operation of the

system. Please observe the warnings and notes printed in this

manual and on the unit.

The AxioCam MR CCD camera has been manufactured and tested by Carl

Zeiss according to the regulations specified in CE and cUL and has left the

manufacturer’s premises in perfect working order.

In order to ensure that this condition is maintained and to avoid any risks

when operating the system, the user must comply with any notes and

warnings contained in this manual.

Installation of the AxioCam MR

2 B 48-0021 e 10.2003

Exemption from statutory liability for accidents

The manufacturer shall be exempt from statutory liability for accidents should

the operator fail to observe the safety regulations.

Limitation of liability

No warranty shall be assumed by Carl Zeiss during the warranty period if the

equipment is operated without observing the safety regulations. In any such

case, Carl Zeiss shall be exempt from statutory liability for accidents resulting

from such operation.

Exemption from warranty

Carl Zeiss shall be exempt from any warranty obligations should the user fail

to observe the safety regulations. Carl Zeiss only guarantees the safety,

reliability, and performance of the system if the following safety regulations

are closely observed.

 The electrical installations of the room where the system is to be set up

must conform to IEC requirements.

Warning

Any interruption of the PE conductor, either internally or externally, or

removal of the earthed conductor will make the system unsafe to use.

Any intentional interruption of the earthed conductor is illegal.

Attention

To interrupt the power supply simply disconnect from the mains.

 Setup, expansions, re-adjustments, alterations, and repairs must only be

carried out by persons who have been authorized by Carl Zeiss.

 Use only those cables supplied by Carl Zeiss.

 Do not allow any cables, particularly power cords, to trail across the floor,

where they can be snagged by people walking past.

Installation of the AxioCam MR

AxioCam MR Installation + Reference 3

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Please ensure the notes described below are adhered to when setting up and

operating the AxioCam MR camera.

 All connectors must be firmly and securely attached.

 Please protect all camera cables from excessive heat (e.g. halogen lamps,

microscope fluorescence illumination).

 Only use the camera in a clean and dry location. If voltage is applied in an

environment with over 70% humidity, the Piezo actuator insulating

resistance may be reduced.

 The camera must be protected against mechanical impact. External

damage may affect the operation of inner components.

 Keep chemicals and fluids away from the camera. To avoid the risk of fire,

do not use near inflammable liquids or gases.

 Make sure there is sufficient ventilation of the camera head. Avoid direct

exposure to sunlight and locations near to heat sources (radiators, stoves).

Overheating can cause noisy images.

 The camera housing can be cleaned using normal microscope cleaning

material.

If a repair is necessary send the camera including all cables with a detailed

description of the fault to:

Carl Zeiss

Service Mikroskopie

Abt. MS-LO2 Tel.: (++49) 551 5060 428

Königsallee 9-21 Fax: (++49) 551 5060 325

D-37081 Göttingen eMail: mikro.reparaturen@zeiss.de

Installation of the AxioCam MR

4 B 48-0021 e 10.2003

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We strongly recommend that all users save the data they create, such as

images, measurement data, archives, reports, forms and documents, at

regular intervals on an external medium. Otherwise it cannot be excluded that

access to this data may be lost as a result of operational errors or hardware

defects. Carl Zeiss accepts no liability for consequential damage resulting from

insufficient data protection.

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The appropriate system requirements relating to hard- and software

equipment can be found in the file "Installation Manual.PDF" on the actual

product CD.

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 AxioCam MR camera head

 PCI interface board

 Data cable (data transfer between camera and PCI interface board)

 CD-ROM with device driver for AxioVision

 This Installation Guide

Camera, PCI interface board, data cable

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