Olympus BX45 Bedienungsanleitung

INSTRUCTIONS

BX45

ERGONOMIC MICROSCOPE

A X 7 4 8 3

This instruction manual is for the Olympus Ergonomic Microscope Model BX45. To ensure the

safety, obtain optimum performance and to familiarize yourself fully with the use of this microscope,

we recommend that you study this manual thoroughly before operating the microscope. Retain this

instruction manual in an easily accessible place near the work desk for future reference.

This publication is printed on 100% recycled paper

BX45

CONTENTS

4-5

TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION PROCEDURE

Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to assemble

the microscope yourself, please read section 8, “ASSEMBLY” (pages 28 to 30) carefully.

IMPORTANT — Be sure to read this section for safe use of the equipment. —

1 NOMENCLATURE

3 USING THE CONTROLS

3-1 Base .................................................................................................................................................................................................................... 8-9

3-2 Focusing Block ...................................................................................................................................................................................... 10

3-3 Stage............................................................................................................................................................................................................ 11- 12

3-4 Observation Tube ...................................................................................................................................................................... 13 - 14

1Placing the Specimen; 2Adjusting the X- and Y-Axis Knob Tension;

3 Rotating the Stage

3-5 Condenser .......................................................................................................................................................................................... 15-16

1Centering the Condenser; 2Compatibility of Objectives and Condensers

3-6 Immersion Objectives .................................................................................................................................................................. 17

1Using Immersion Objectives

3-7 Objectives with Correction Collar .............................................................................................................................. 17

3-8 Marker (U-MARKER)........................................................................................................................................................................ 18

4 OBSERVATION METHODS

4-1 Transmitted Light Phase Contrast Observation .......................................................................... 18-19

4-2 Transmitted Light Darkfield Observation......................................................................................................... 20

4-3 Transmitted Light Simple Polarization Observation......................................................................... 21

5 TROUBLESHOOTING GUIDE

6 SPECIFICATIONS

6-7

8-18

18-21

22-24

25-26

1Voltage Indication; 2Using the Light Intensity Preset Switch; 3 Using the Filters

1Replacing the Fine Adjustment Knob;

2Adjusting the Coarse Adjustment Knob Tension;

3 Pre-focusing Lever

1Adjusting the Interpupillar Distance; 2Adjusting the Diopter;

3Using the Eye Shades; 4 Using Eyepiece Micrometer Disks;

5Adjusting the Tilt

1-3

7 OPTICAL CHARACTERISTICS 27

8 ASSEMBLY

— See this section for the replacement of the light bulb. —

28-30

■PROPER SELECTION OF THE POWER SUPPLY CORD ................................................................... 31-32

BX45

1. After the equipment has been used in an observation of a specimen that

is accompanied with a potential of infection, clean the parts coming in

contact with the specimen to prevent infection.

· Moving this product is accompanied with the risk of dropping the speci-

men. Be sure to remove the specimen before moving this product.

· In case the specimen is damaged by erroneous operation, promptly

take the infection prevention measures.

2. Install the microscope on a sturdy, level table or bench so as not to block

the air vents on the underside of the base.

Do not place the microscope on a flexible surface, as this could result in

blocking the air vents and cause overheating or a fire.

3. To prevent obstruction of the natural convection-based air cooling of the

microscope, make sure to leave at least 10 cm of free space between

walls or other objects, and the left, right and rear sides of the microscope

and the lamp socket when installing the microscope.

4. To avoid potential shock hazards and burns when replacing the light

bulb, set the main switch @ to “ ” (OFF) then disconnect the power

cord from the wall outlet in advance. Whenever you replace the bulb

during use or right after use, allow the lamp socket ² and bulb to cool

before touching. (Figs 1 & 2)

IMPORTANT

SAFETY PRECAUTIONS

Fig. 1

Fig. 2

This microscope employs a UIS2/UIS (Universal Infinity System) optical design, and should be used only

with UIS2/UIS eyepieces, objectives and condensers for the BX2 series. (Some of the modules designed for

the BX series and objectives/eyepieces for the UIS series are also usable. For details, please consult Olympus

or the catalogues.) Less than optimum performance may result if inappropriate accessories are used.

Designated bulb 6V30WHAL (PHILIPS 5761)

# The microscope also incorporate a fuse (this should be replaced by

the manufacturer or authorized agent).

5. Always use the power cord provided by Olympus. If no power cord is

provided, please select the proper power cord by referring to the section

“PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of

this instruction manual. If the proper power cord is not used, product

safety and performance cannot be guaranteed.

6. Always ensure that the grounding terminal of the microscope and that

of the wall outlet are properly connected. If the equipment is not

grounded/earthed, Olympus can no longer warrant the electrical safety

and performance of the equipment.

7. Never insert metallic objects into the air vents of the microscope frame

as this could result in electrical shock, personal injury and equipment

damage.

8. The power cord may be melt by the heat of lamp socket if the cord

comes in contact with it. Distribute the power cord at an enough dis-

tance from the lamp socket.

Symbol Explanation

Safety Symbols

Indicates that the surface becomes hot, and should not be touched with bare hands.

Before use, carefully read the instruction manual. Improper use could result in personal injury to

the user and/or damage to the equipment.

Indicates that the main switch is ON.

Indicates that the main switch is OFF.

Warnings

Warning engraving

position Lamp socket

(Warning against high temperature)

Warning sticker

position

Microscope frame rear panel

(Warning against high temperature)

Fig. 3

1Getting Ready

The following symbols are found on the microscope. Study the meaning of the symbols and always use the equip-

ment in the safest possible manner.

Warning engraving/stickers are placed at parts where special precaution is required when handling and using the

microscope. Always heed the warnings.

Should warning stickers become soiled, peeled off, etc., contact Olympus for replacement.

1. A microscope is a precision instrument. Handle it with care and avoid

subjecting it to sudden or severe impact.

2. Do not use the microscope where it is subjected to direct sunlight, high

temperature and humidity, dust or vibrations. (For the operating condi-

tions, refer to section 6, “SPECIFICATIONS”.)

3. When moving the microscope, remove the specimen and modules that

may drop during transport and carefully carry it with the grasping part

on the rear of the arm and the base as shown in Fig. 3 (Weight: approx.

14 k g ) .

# Damage to the microscope will occur if you grasp it by the stage,

coarse/fine adjustment knob or binocular section of the observa-

tion tube.

4. The BX45 can be used with only one intermediate attachment.

BX45

1. To clean the lenses and other glass components, simply blow dirty away using a commercially available blower and wipe

gently using a piece of cleaning paper (or clean gauze).

If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute

alcohol.

Since the absolute alcohol is highly flammable, it must be handled carefully.

Be sure to keep it away from open flames or potential sources of electrical sparks –– for example, electrical

equipment that is being switched on or off.

Also remember to always use it only in a well-ventilated room.

2. Do not attempt to use organic solvents to clean the microscope components other than the glass components. To clean

them, use a lint-free, soft cloth slightly moistened with a diluted neutral detergent.

3. Do not disassemble any part of the microscope as this could result in malfunction or reduced performance.

4. When not using the microscope, keep it covered with a dust cover.

5. When disposing of the microscope. Check the regulations and rules of your local government and be sure to

observe them.

2Maintenance and Storage

NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device,

pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection

against harmful interference when the equipment is operated in a commercial environment. This

equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in

accordance with the instruction manual, may cause harmful interference to radio communications.

Operation of this equipment in a residential area is likely to cause harmful interference in which case

the user will be required to correct the interference at his own expense.

FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance

could void the user’s authority to operate the equipment.

This device complies with the requirements of directive 98/79/EC concerning in vitro diagnostic

medical devices. CE marking means the conformity to the directive.

The following symbols are used to set off text in this instruction manual.

: Indicates that failure to follow the instructions in the warning could result in bodily harm to the

user and/or damage to equipment (including objects in the vicinity of the equipment).

# : Indicates that failure to follow the instructions could result in damage to equipment.

} : Indicates commentary (for ease of operation and maintenance).

3Caution

If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition,

the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.

NOMENCLATURE

}If you have not yet assembled the microscope, read section 8, “ASSEMBLY” (pages 28 to 30).

Interpupillary distance adjustment scale

(Page 13)

Diopter adjustment ring

(Page 13)

Slider insertion slot

Slide holder (Page 11)

Aperture iris diaphragm ring

(Page 16)

Y-axis knob (Page 12)

X-axis knob (Page 12)

Turret (Page 5)

Brightness adjustment knob (Page 8)

(Lamp voltage adjustment knob)

Field iris diaphragm ring (Page 15)

Marker

U-MARKER

Allen screwdriver

(accommodation position)

Condenser centering screws

Main switch (Page 1)

Voltage indicator LED

Light intensity preset

switch (Page 8)

Light intensity preset

adjustment screw

(Page 8)

Coarse adjustment tension adjustment ring (Page 10)

Filter slider (Page 9)

(accommodation positions)

BX45

Coarse adjustment knob (Page 10)

Pre-focusing lever (Page 10)

Fine adjustment knob (Page 10)

(Detachable)

Fine adjustment knob rubber cap

(Page 10)

Top lens swing-out lever (Page 15)

Condenser height adjustment knob (Page 15)

Turret View when disassembled from the microscope frame.

Aperture iris diaphragm (Page 16)

Filter mount (Bottom side) (Page 9)

Mountable filters (32 mm diameter)

· 32C (provided), (32LBD)*

· 32LND6

· 32LND25

· 32IF550

*Refer to Page 9 for precautions

on use.

Phase contrast/darkfield ring mount (Pages 18 - 20)

Mountable devices

Optical devices for phase contrast

· BX45-PH1

· BX45-PH2

· BX45-PH3

Optical devices for darkfield

· BX45-DFA

(Page)

TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION

PROCEDURE

}As the phase contrast, darkfield and simplified polarized light observations using transmitted light requires preparation using

optical devices such as an analyzer or polarizer, they will additionally be detailed in section 4, “OBSERVATION”.

Set the main switch to “ I” (ON) and adjust the

brightness.

(Controls Used)

(P. 8)

@Main switch

²Brightness adjustment knob

Select the BF (brightfield) light path by turning

the turret. ³Turret

Select the light path (trinocular tube). |Light path selector knob

Place the specimen on the stage. ƒSlide holder

…X-/Y-axis knobs

Engage the 10X objective in the light path.

Then engage the top lens in the light path.

†Revolving nosepiece

‡Top lens swing-out lever

Bring the specimen in focus. ŠCoarse/fine adjustment knobs

Adjust the interpupillary distance.

Adjust the diopter.

Adjust the light axis.

‰Binocular tube

‹Diopter adjustment ring

ŒCondenser height adjustment

knob

™Condenser centering screws

Adjust the aperture iris and field iris diaphragms. šAperture iris diaphragm ring

›Field iris diaphragm ring

Engage the desired objective in the light path

and bring the specimen in focus.

†Revolving nosepiece

‡Top lens swing-out lever

Insert the required filters. œFilters

Adjust the brightness. ²Brightness adjustment knob

Start observation.

(P. 11)

(P. 12)

(P. 15)

(P. 13)

(P. 15)

(P. 10)

(P. 9)

(P. 15)

(P. 16)

(P. 15)

(P. 8)

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