Olympus IX73 Bedienungsanleitung

IX73

INSTRUCTIONS

RESEARCH INVERTED MICROSCOPE

This instruction manual is for the Olympus research inverted microscope model IX73.

To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use

of this microscope, we recommend that you study this manual thoroughly before operating the

microscope.

Retain this instruction manual in an easily accessible place near the work desk for future reference.

A X 8 1 5 7

In accordance with European Directive 2002/96/EC on Waste Electrical and Electronic Equip-

ment, this symbol indicates that the product must not be disposed of as unsorted municipal

waste, but should be collected separately.

Refer to your local Olympus distributor in EU for return and/or collection systems available in

your country.

1Intended use...............................................................................................................................................................................................4

2Conformity of the System................................................................................................................................................................5

3Handling Precautions..........................................................................................................................................................................6

4Maintenance and Storage..............................................................................................................................................................6

1 MODULE NOMENCLATURE .................................................................................................7

2 NOMENCLATURE ..........................................................................................................................8

3 TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION PROCEDURE .12

4 USING THE CONTROLS..........................................................................................................14

4-1 Power Supply Unit and Microscope Frame.......................................................................................14

1Turning Power On, Adjusting the Brightness .................................................................................................................14

2Light Path Selection..............................................................................................................................................................................15

Coded intermediate magnification changer IX3-CAS....................................................................................................... 15

4Frame Fix Plate IX3-FP.......................................................................................................................................................................15

5Dust tray..........................................................................................................................................................................................................16

4-2 Focusing Block............................................................................................................................................................17

1Rotation Direction of the Coarse/Fine Adjustment Knobs..........................................................17

2Adjusting the Coarse Adjustment Knob Tension.......................................................................................................17

3Detaching the Fine Adjustment Knob..................................................................................................................................17

4Pre-focusing Lever.................................................................................................................................................................................17

4-3 Stage....................................................................................................................................................................................18

1Placing the Specimen........................................................................................................................................................................18

2Moving the Specimen.........................................................................................................................................................................21

3Connecting the Grounding Wire...............................................................................................................................................22

IMPORTANT -- Be sure to read this section for safe use of the equipment. -- 1-6

4-4 Observation Tube......................................................................................................................................................23

1Adjusting the Interpupillary Distance....................................................................................................................................23

2Adjusting the Diopter...........................................................................................................................................................................23

3Using the Eye Shades........................................................................................................................................................................24

4Mounting the Eyepiece Micrometer Disk..........................................................................................................................24

5Selecting the Light Path of the Trinocular Tube...........................................................................................................24

6Adjusting the Tilt (U-TBI90).............................................................................................................................................................24

4-5 Illumination Column (IX3-ILL)..........................................................................................................................25

1Tilting the Illumination Column...................................................................................................................................................25

2Mounting Filters........................................................................................................................................................................................26

3Using the Field Iris Diaphragm...................................................................................................................................................27

Adjusting the Condenser Height Adjustment Knob Tension...................................................................................................................27

5Condenser refocusing stopper..................................................................................................................................................27

4-6 Condenser ......................................................................................................................................................................28

1Centering the Condenser................................................................................................................................................................28

2Using the Aperture Iris Diaphragm .........................................................................................................................................30

4-7 Oil- or Water-Immersion Objective ..........................................................................................................31

1Using Oil- or Water-Immersion Objective.........................................................................................................................31

4-8 Objectives with Correction Collar...............................................................................................................32

5 OTHER OBSERVATION METHODS.................................................................................33

5-1 Phase Contrast Observation...........................................................................................................................33

1Phase Contrast Optical Elements and Applicable Objectives.......................................................................33

2Attaching the Phase Contrast Optical Elements........................................................................................................34

3Centering the Phase Contrast Ring Slit..............................................................................................................................36

5-2 Differential Interference Contrast Observation................................................................................37

1DIC Optical Elements, Applicable Objectives and DIC Sliders......................................................................37

2Attaching the DIC Optical Elements......................................................................................................................................38

3Attaching the Analyzer and DIC Slider ................................................................................................................................39

4Attaching the Polarizer (IX-LWPO)............................................................................................................................................40

5Cross-Nicol Adjustment....................................................................................................................................................................41

6Observation Method ............................................................................................................................................................................42

5-3 Simplified Polarized Light Observation .................................................................................................43

1Attaching the Analyzer and Polarizer....................................................................................................................................43

2Observation Method ............................................................................................................................................................................43

5-4 Reflected Light Fluorescence Observation (Separate Manual) ......................................44

5-5 Relief Contrast Observation (Separate Manual) ...........................................................................44

6 CAMERA RECORDING.............................................................................................................45

1Camera Adapter ......................................................................................................................................................................................45

2Changing of Light Path......................................................................................................................................................................45

3Selecting the Camera Adapter Magnification...............................................................................................................45

4Installing the Camera Adapter....................................................................................................................................................46

7 TROUBLESHOOTING GUIDE ..............................................................................................47

8 SPECIFICATIONS ...........................................................................................................................49

9 ASSEMBLY...........................................................................................................................................50

9-1 Assembly Diagram..................................................................................................................................................50

9-2 Detailed Assembly Procedures....................................................................................................................51

1Attaching the revolving nosepiece.........................................................................................................................................51

2Mounting the Illumination Column..........................................................................................................................................52

3Attaching the Halogen Bulb..........................................................................................................................................................52

4Mounting the Lamp Housing........................................................................................................................................................53

5Mounting to Microscope Deck...................................................................................................................................................54

Caution

If the equipment is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition,

the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.

The following symbols are used to set off text in this instruction manual.

CAUTION : Indicates a potentially hazardous situation which, if not avoided, may result in minor or

moderate injury or damage to the equipment or other property. It may also be used to

alert against unsafe practices.

} : Indicates commentary (for ease of operation and maintenance).

6Attaching the Stage..............................................................................................................................................................................55

7Attaching the Objectives..................................................................................................................................................................57

8Attaching the Condenser Refocusing Stopper............................................................................................................57

9Attaching the Condenser ................................................................................................................................................................58

10 Attaching the Observation Tube................................................................................................................................................59

11 Attaching the Eyepieces..................................................................................................................................................................60

12 Using the Accessory Mounting Holes..................................................................................................................................60

Using the Frame Fix Plate (IX3-FP)................................................................................................................................................................... 61

14 Connecting the Cables .....................................................................................................................................................................62

15 Cable Distributions................................................................................................................................................................................63

16 Cable Cover.................................................................................................................................................................................................64

10 LAMP HOUSING INSPECTION SHEET .....................................................................65

PROPER SELECTION OF THE POWER SUPPLY CORD ............................................................ ................................................... 66, 67

IX73

IMPORTANT

This microscope employs UIS2 optical design.

For the applicable modules, please consult Olympus or refer to the latest brochures or Olympus website. Less than

optimum performance may result if inappropriate module combinations are used.

Configuration of Instruction Manuals

Since this microscope is expandable to a variety of systems, separate instruction manuals are prepared so that the

user has to read only the manuals according to the user’s own system.

Manual Name Main contents

IX73

(this instruction manual)

Observation procedures including transmitted light brightfield, phase

contrast and DIC observations

TH4 Halogen lamp power supply unit

REFLECTED FLUORES-

CENCE SYSTEM Reflected light fluorescence observation

IX2-MLWCD Mid-long working distance condenser

U-AW Motorized attenuator wheel

BX3-SSU Scanning stage with ultrasonic

IX2-GS Gliding stage

IX3-RSPC Right side port with C-mount

U-CBF Control box for fast filter wheel and shutter

IX3-CBM/U-HSCBM Configuration and usage of the modules available in the IX73 motor-

ized system or the IX73 motorized coded system.

U-LEDPS LED Power supply

U-CB5S Control Box for coded function

Be sure to work on the following tasks first after finishing the unpacking.

Releasing the Transport Lock

aReleasing the transport lock of the focusing mechanism

Never attempt to rotate the focusing knob without removing the

clamping plate. Otherwise, the focusing mechanism may be

damaged.

Loosen and remove the screws aof the clamping plate using the

Allen wrench provided with the microscope.

} Keep the clamping plate and screws in a safe place because it will

be used again when the microscope is transported next time.

Releasing the transport lock of the light path selector

Never attempt to operate the light path selector without remov-

ing the transport lock screw. Otherwise, the light path selector

mechanism may be damaged.

Loosen and remove the transport lock screw busing the Allen

screwdriver provided with the microscope.

} Keep the lock screw in a safe place because it will be used again

when the microscope is transported next time.

CAUTION

SAFETY PRECAUTIONS

1. If potentially infectious samples may be observed, use protective

gloves or other protective means to prevent the skin from contacting

with samples directly.

After observation, be sure to clean the portion contacted with

samples.

• Moving this device is accompanied with the risk of dropping the

samples. Be sure to remove the samples before moving this device.

• In case the samples are damaged by erroneous operation,

promptly take the infection prevention measures.

• Follow the procedures described in "Handling Precautions" (see

page 6) prior to using the accessories of this device. Otherwise,

the stability of the device will be lost and the dropped samples

will cause the possibility of infection.

• When you maintain the device which may have contacted with

potentially infectious samples, be sure to wear protectors such as

gloves, or clean the device prior to operation.

• Before disposing of the device contacted with potentially infec-

tious samples, be sure to follow the regulations and rules of your

local government.

2. Be careful not to have your hand caught between the bottom of the

revolving nosepiece and the microscope frame.

3. The microscope is not provided with a waterproof mechanism.

Therefore, if culture liquid or water is spilt on the stage, revolving

nosepiece or microscope frame, damage to the equipment or an

electrical shock may result. Immediately wipe the liquid or water off if

it is spilt on them.

4. The microscope is not provided with a dust-proof mechanism.

5. Install the microscope on a sturdy, level table or bench.

6. When moving the microscope, remove the modules that may drop

including the specimen from the microscope in advance, then care-

fully carry the microscope frame by holding the base (front edge) a

and the grasping part below the illumination column b.

7. When carrying the microscope to a different place, it is also recom-

mended to disconnect all cables and modules from the microscope

frame.

When transporting it, also engage the transport lock mechanisms

and package it sufficiently.

Also be careful against slipping of hands during carrying.

8. Damage to the microscope will occur if you grasp it by other parts

including the stage, focusing knob, etc.

9. This microscope is not equipped with laser safety mechanisms. The

user should assume liabilities for any consequence of user modifica-

tion including introduction of the use of a laser beam.

10.

If laser equipment is attached to the product you have purchased,

this Instruction Manual is not effective. Follow the Instruction Manual

provided with the laser equipment to be attached.

11 The surfaces of the lamp housing will become extremely hot dur-

ing operation. When installing the microscope, make sure to allow

ample free space (10 cm or more) around and in particular above

the lamp housing.

12. When installing the microscope, route the power cord away from the

lamp housing. Should the power cord come in contact with a hot

part, the power cord could melt and cause electric shock.

IX73

13. After operation or in case of abnormality, be sure to disconnect the

power cord from the connector on the products or from the outlet.

14. To avoid potential shock hazards and burns when replacing the

lamp bulb, set the main switch to “\” (OFF) then disconnect the

power cord from the wall outlet in advance. Whenever you replace

the bulb during use or right after use, allow the lamp housing c

and bulb to cool before touching.

Designated bulb 12V100WHAL-L (PHILIPS 7724)

15. Do not permit tools or metal fragments to get into air vents, or other

apertures. Doing so could cause failure of the microscope or electric

shock to the user.

16. The standard service life of the lamp housing is eight (8) years of use

or 20,000 hours of total power ON period, whichever is the shorter

period. For details, see the Inspection Sheet on page 65.

17. During the fluorescent observation, be sure to attach the antiglare

plate to the microscope to protect your eyes.

18. If you feel bright during observation through eyepieces, darken the

illumination light by adjusting the brightness of the light source or

using the ND filter. Use the equipment according to the regulations

for workers health and safety, if any.

– Europe: 2006/25/EC Directive for protecting workers from risks

caused by physical factors (artificial optical radiation).

19. Do not look directly at the light from the objective lens or the specu-

lar reflection light of the specimen.

20.Be careful invisible wavelength light (ultraviolet or infrared) may be

emitted depending on illumination methods.

21. If the skin is exposed to the light from the objective lens for a long

time, it may cause burns. Be sure to avoid it.

22.Do not place flammable gas or liquid close to the light from the

objective lens. It may cause fire.

23.This device complies with the emission and immunity requirements

described in IEC61326 series.

24.The electromagnetic environment should be evaluated prior to op-

eration of this device. Do not use this device in close proximity to the

sources of strong electromagnetic radiation to prevent interference

with the proper operation.

25.Always use the power cord provided by Olympus. If no power cord

is provided, please select the proper power cord by referring to the

section “PROPER SELECTION OF THE POWER CORD” at the end

of this instruction manual. If the proper power cord is not used, the

safety and EMC performance of the device can not be assured.

26 Always connect the power cord correctly and ensure that the

grounding terminal of the device and that of the wall outlet are prop-

erly connected. If the device is not grounded, our intended electric

safety and EMC performance of the device can not be assured.

27. It is discommended to stare at the light directly from the LED light

source composed in this product for long hours as this could dam-

age your eye. Use the equipment in accordance with the regulations

for Workers Health and Safety, if any.

This device has been designed to be used to observe magnified images of specimens in various routine work and

research applications.

Do not use this device for any purpose other than its intended use.

This product complies with the requirements of directive 98/79/EC concerning in vitro diagnos-

tic medical devices. CE marking means the conformity to the directive.

EN61326-1 defines two categories according to the location for use.

Class A : Equipment suitable for use in establishments other than domestic, and those directly connected to a low volt-

age power supply network which supplies buildings used for domestic purposes.

Class B : Equipment for use in domestic establishments, and in establishments directly connected to a low voltage

power supply network which supplies buildings used for domestic purposes.

This product is applied Class A. Some interference may occur if this system is used in domestic location.

USA: CAUTION:

Federal law restricts this device to sale by or on the order of an appropriately licensed healthcare practitioner.

Caution labels

Caution labels are placed at parts where special precaution is required when handling and using the microscope.

Always pay attentions to the caution labels.

Positions of caution labels Lamp housing

Back side of microscope frame

If the caution label becomes dirty or is peeled off, contact Olympus for replacement.

Safety Symbols

The following symbols are found on the microscope. Study the meaning of the symbols and always use the equip-

ment in the safest possible manner.

Symbol Explanation

Indicates that the surface becomes hot, and should not be touched with bare hands.

Indicates a non-specific general hazard. Follow the description given after this symbol or

in the instruction manual.

Indicates that the main switch is ON.

Indicates that the main switch is OFF.

1Intended use

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