Zeiss LSM 880 Bedienungsanleitung

LSM 880 and LSM 880 NLO

Notes on device safety and

installation requirements

November 2014

ZEISS LSM 880

II 000000-2071-466 11/2014 V_05

Knowledge of this manual is required for the operation of the instrument. Would you therefore please

make yourself familiar with the contents of this manual and pay special attention to hints concerning the

safe operation of the instrument.

The specifications are subject to change; the manual is not covered by an update service.

communication of its contents are not permitted. Violations will entail an obligation to pay

compensation.

Issued by Carl Zeiss Microscopy GmbH

Carl-Zeiss-Promenade 10

07745 Jena, Germany

microscopy@zeiss.com

www.zeiss.com/microscopy

CHAPTER 1 - NOTES ON DEVICE SAFETY

LSM 880 Contents ZEISS

11/2014 V_05 000000-2071-466 1

CHAPTER 1 NOTES ON DEVICE SAFETY

CONTENTS

Page

1General ................................................................................................................2

2Regulations..........................................................................................................3

3Power Requirements ..........................................................................................4

4Physical Dimensions............................................................................................8

5Environmental Requirements.............................................................................9

6Notes on Setting up the Microscope System ..................................................10

7Warning and Information Labels .....................................................................12

8Notes on Handling the Laser Components and Illumination Systems ..........23

9Notes on Care, Maintenance and Service........................................................26

9.1 Exchanging Fuses ...........................................................................................................26

Cleaning ........................................................................................................................27

9.2 27

9.3 Maintenance of the Liquid Cooling System .....................................................................27

9.4 Count on Service in the True Sense of the Word .............................................................28

10 User Interface ....................................................................................................29

10.1 Mounting and Dismounting Lamps, T-PMT and Switching Mirror.....................................29

10.2 Mounting and Dismounting the BiG.2.............................................................................31

10.3 Changing the Filter Modules in the BiG.2........................................................................33

10.4 External Connectors on BiG.2 .........................................................................................34

10.5 Using the GaAsP NDD 2 Channels on Axio Examiner Stand for Multiphoton Imaging .......35

CHAPTER 1 - NOTES ON DEVICE SAFETY

ZEISS General LSM 880

2 000000-2071-466 11/2014 V_05

1General

The LSM 880 and LSM 880 NLO laser scanning microscopes, including their original accessories and

compatible accessories from other manufacturers, may only be used for the purpose of microscopic and

spectroscopic techniques.

Laser Scanning Microscopes (LSM) are intended for high resolution imaging of biological or material

samples, whereby in contrast to wide field microscopy the specimen is illuminated raster-fashion with a

focused laser beam and the optical arrangement prevents light from out-of-focus regions of the

specimen contributing to image formation.

Installation and commissioning of the LSM 880 system must be performed by authorized ZEISS

service staff. The system should not be used prior to instruction by a ZEISS representative.

The manufacturer will not assume liability for any malfunction or damage caused by anything

other than the intended use of the LSM 880 system or individual modules or parts of it, nor by

any repair or other service operation performed or attempted by persons other than duly

authorized service staff. Any such action will invalidate any claim

under warranty, including

parts not directly affected by such action. This also includes the modification of the system

computer with new cards, etc. by the user. The use of a camera at the base port of

Axio Observer.Z1 SP stands with motorized beam path switching is not allowed for reasons of

laser safety. Any manipulation will result in the loss of warranty of laser safety.

Please read also the notes on device safety and manuals of the microscope, the HBO, the HAL, the HXP,

and additional optional devices, if ordered, as the UV Laser, the piezo focusing device, the heating

inserts, the Ti:Sa laser and the OPO (optical parametric oscillator).

As the system is largely operated via menus on a computer, you should be familiar with the principles of

the WINDOWS operating system and its graphical user interface. The respective manuals are supplied

together with the programs.

The LSM 880 system is a device that belongs to laser hazard class 3B. The system is equipped

with safety interlocks that comply with laser hazard class 3B and 4. If equipped with a Ti:Sa

laser (see list in section 8), the LSM 880 system is a device that belongs

to laser hazard class 4.

WHO recommendations concerning health and industrial protection when handling laser

devices must be observed. The operator of the unit must also observe all and any relevant

statutory accident prevention regulations.

The user is referred to the safety data sheet provided

together with the manual.

The LSM 880 system meets the EMC requirements for EN 55011 Class A (intended use in industrial

environment). If the LSM is operated in a residential area or in a small trade area other devices may be

influenced by conducted or radiated disturbance. In this case special EMC measures are required.

CHAPTER 1 - NOTES ON DEVICE SAFETY

LSM 880 Regulations ZEISS

11/2014 V_05 000000-2071-466 3

2Regulations

Extensive knowledge of the hardware / the system is indispensable for safe operation of the LSM 880

system.

Read these operating instructions and all device publications belonging to the system

conscientiously before operating the LSM 880 system! You can obtain additional information on

the hardware configuration delivered and on optional system extensions from the manufacturer or

via the service hotline.

The LSM 880 system has been designed, built and tested in conformity with the following regulations

and guidelines:

−DIN EN 61010-1 (IEC 61010-1) "Safety requirements for electrical equipment for measurement,

control and laboratory use", taking relevant CSA and UL specifications into account,

−DIN EN 60825-1 (IEC publication 60825-1) "Safety of laser equipment",

−21 CFR §1040.10: "Performance Standards for light emitting products – laser products",

−DIN EN 61326-1 "Electrical equipment for measurement, control and laboratory use – EMC

requirements",

−Low voltage directive: 2006/95/EC,

−EMC directive: 2004/108/EC, KN 61000-6-2, KN 11.

−EMC User notice for Korean only:

사용자안내문

기종별사용자안내문

A급기기

(업무용 방송통신기자재)

이기기는 업무용(A급) 전자파적합기기로서 판매자 또는

사용자는이 점을 주의하시기 바라며, 가정외의 지역에서 사용하는

것을 목적으로 합니다.

The company works according to a certified Environment Management System according to ISO 14001.

The Product was developed, tested and produced in accordance with the valid regulations and guidelines

for environmental law of the European Union.

The product and its accessories have been classified as instrument category 9 (laboratory equipment or

comparable standard). The product and its accessories agree with the EU-regulations 2011/65/EC (RoHS)

and 2012/19/EC (WEEE), if applicable for the product.

ZEISS has installed a process for taking back and recycling the instruments within the member states of

the European Union, which takes care of the appropriate utilization according to the said EU guidelines.

For details on the disposal and recycling please refer to your relevant ZEISS sales or service organization.

The product must not be disposed in the household waste or through the municipal disposal

organizations. In case of resale the seller is obliged to inform the buyer that the product has to be

disposed according to the said regulations.

CHAPTER 1 - NOTES ON DEVICE SAFETY

ZEISS Power Requirements LSM 880

4 000000-2071-466 11/2014 V_05

3Power Requirements

The LSM 880 system comes with a mains power supply cord and plug, either CEE red

(3/N/PE 400/230 V/16A), or NEMA L 14-30P (2/N/Ground 120/240 V/30 A), and with the matching

mains socket outlet.

The mains socket outlet must be equipped with a fuse having minimum tripping characteristic C

according to IEC/EN 60898.

Line voltage 3/N/PE 400/230 V AC (±10 %) 2/N/Ground 240/120 V AC (±10 %)

Line frequency 50...60 Hz 50...60 Hz

LSM incl. VIS laser:

Max. current

Power

3 phases at 16 A

Phase 1 = 600 VA max.

Phase 2 = 500 VA max.

Phase 3 = 1500 VA max.

2 phases at 25 A

Phase 1 = 800 VA max.

Phase 2 = 1600 VA max.

Power consumption 2100 VA max. 2100 VA max.

Power consumption

Ti:Sa Laser 1

Ti:Sa Laser 2

OPO

800 VA max.

600 VA max.

800 VA max.

600 VA max.

Class of protection I I

Type of protection IP 20 IP 20

Overvoltage category II II

Pollution degree 2 2

Heat emission (without

Ti:Sa or OPO laser) 2000 W max. 2000 W max.

EMC test according to DIN EN 61326-1 (05/2010)

1. Noise emission according to CISPR 11 / DIN EN 55011 (04/2011)

2. Noise immunity according to table 2 (industrial sector)

The LSM contains sensitive electronics. A few seconds of faulty power can damage parts of the

electronics. The system is therefore equipped with a surge guard, a device that protects the

electronics of the LSM from strong electrical power fluctuations like overvoltage, lightning or other

external influences.

The surge guard is located within the electrical connection box. It works once to protect the

system, then it has to be exchanged. Within warranty, service contracts or any other service visit

ZEISS service will check the functionality of the surge guard and provide a new device if necessary.

Nevertheless, in the rare case of a voltage surge on the power supply line (e.g. from indirect

lightning stroke), a momentary interruption of the acquisition system's functionality is possible. This

is no defect. Acquisition can be continued after starting the ZEN software again. Sample position is

maintained and no damage occurs to the system. A possible protective means to minimize the

probability of a temporary disruption is a transient voltage protector in your facility's power system.

CHAPTER 1 - NOTES ON DEVICE SAFETY

LSM 880 Power Requirements ZEISS

11/2014 V_05 000000-2071-466 5

The Ti:Sa laser(s) and OPO need to have a separate electrical connection according to the

instructions of the manufacturer.

When operating the main switch of the LSM 880 NLO the Ti:Sa lasers and the OPO are not

switched on or off.

Fig. 1 Fuses F1/F2 of the external laser module

CHAPTER 1 - NOTES ON DEVICE SAFETY

ZEISS Power Requirements LSM 880

6 000000-2071-466 11/2014 V_05

Fig. 2 Power connector for the LSM 880 system including NLO system and set ups with

laser module UV and components. Free/reserve outlets may be used to supply power to

additional equipment. No more than 1 A can be provided by each outlet.

CHAPTER 1 - NOTES ON DEVICE SAFETY

LSM 880 Power Requirements ZEISS

11/2014 V_05 000000-2071-466 7

The door interlock interface (Fig. 3/

) is covered

with a green plug to bypass a door interlock.

•To use the interface remove the top of the

green plug and the bypass wire.

•Then connect the wires of the door interlock at

the same position.

Two door interlocks can be connected.

The LSM 880 system is controlled by a remote

control. This remote control contains the main

switch for the system and the key switch for the

laser.

•To start the system switch the main switch

(Fig. 4/1) to ON.

•To activate the laser turn the key switch

(Fig. 4/2) to ON position.

Fig. 3 Door interlock interface (1) on the

back of the electronic rack (see

Fig. 2 top)

1Main switch ON/OFF

2Laser key switch

Fig. 4 Remote control of the

LSM 880 system

CHAPTER 1 - NOTES ON DEVICE SAFETY

ZEISS Physical Dimensions LSM 880

8 000000-2071-466 11/2014 V_05

4Physical Dimensions

Length (cm) Width (cm) Height (cm) Weight (kg)

Small passively damped system table 90 75 77 80

Small actively damped system table 90 75 77 90

Large actively damped system table 120 90 77 120

Active anti-vibration table (NLO)

for Mai Tai Laser or Chameleon

180 150 75 200

Active anti-vibration table (NLO)

for two-microscope configuration

250 150 75 400

Periscope for two NLO lasers or

NLO laser with OPO

40 37 70 40

Active anti-vibration table (NLO) for

Laser Chameleon with OPO

180 180 75 410

Scanning Module LSM 880 50 45 22 27

Microscope 50 35 50 20

Electronic Rack with laser units 80 60 65 80

Plug-in unit external laser 70 55 25 10

Laser Module UV 80 60 45 40

Airyscan 40 20 24 12

Fiber optic cable, UV 200

Fiber optic cable, VIS(ible) 250

Cables 250

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